scholarly journals Proliferative Effects of Nitrogen Nanobubbles on Fibroblasts

2021 ◽  
Author(s):  
Hansol Heo ◽  
Junseon Park ◽  
Jeong Il Lee ◽  
Jungho Kim ◽  
Joong Yull Park ◽  
...  
Keyword(s):  
Cell Culture ◽  
Flow Cytometry ◽  
Cell Proliferation ◽  
Image Analysis ◽  
Culture Medium ◽  
Fibroblast Cells ◽  
High Concentration ◽  
Successful Attempt ◽  
Cell Assays ◽  
Mixing Method

Abstract In recent years, the potential of nanobubbles (NBs) for biological activation has been actively investigated. In this study, we investigated the proliferative effects of nitrogen nanobubbles (N-NBs) on fibroblast cells using cell assays with image analysis and flow cytometry. A high concentration of N-NBs (more than 4 × 108 NBs/mL) was generated in Dulbecco’s modified Eagle’s medium (DMEM) using a gas-liquid mixing method. In image analysis, the cells were counted and compared, which showed an 11% improvement in proliferation in the culture medium with N-NBs. In flow cytometry, the decrease in the fluorescence intensity was analyzed, which revealed a 1.5% improvement in proliferation in the culture medium with N-NBs. This study represents the first successful attempt of directly generating quantified NBs in a culture medium for cell culture. The findings suggest that the N-NBs in the culture medium can facilitate cell proliferation.

Study of Cells Attachment Using Impedance Spectroscopy Technique

2011 ◽  
Author(s):  
Anis N. Nordin ◽  
S. M. Arifuzzaman ◽  
Maizirwan Mel ◽  
David Spray ◽  
Ioana Voiculescu
Keyword(s):  
Cell Culture ◽  
Endothelial Cells ◽  
Impedance Spectroscopy ◽  
Culture Medium ◽  
Equivalent Circuit Model ◽  
Electrical Impedance Spectroscopy ◽  
Fibroblast Cells ◽  
Impedance Sensing ◽  
Impedance Measurements ◽  
Mammalian Cell Lines

In this paper we report on the electrical impedance spectroscopy characterization of the attachment of two mammalian cell lines: endothelial cells (RFPEC) and fibroblast cells (V79). For electrical cell–substrate impedance sensing (ECIS) of the endothelial cells a commercially available eight-well cell culture impedance array (ECIS-8W1E) was used. The impedance measurements were recorded with cell culture medium (without cells) and with endothelial cells or fibroblast cells in the culture medium over a frequency range from 100 Hz to 100 kHz. The impedance measurements were compared to the equivalent circuit model. The impedance measurements were also simulated using COMSOL Multiphysics™, a commercially available modeling package.

scholarly journals Time-Dependent Effects of Pre-Aging 3D Polymer Scaffolds in Cell Culture Medium on Cell Proliferation

2012 ◽  
Vol 3 (2) ◽  
pp. 372-381 ◽  
Author(s):  
Kaushik Chatterjee ◽  
Stevephen Hung ◽  
Girish Kumar ◽  
Carl G. Simon
Keyword(s):  
Cell Culture ◽  
Cell Proliferation ◽  
Culture Medium ◽  
Time Dependent ◽  
Cell Culture Medium ◽  
Polymer Scaffolds

scholarly journals Fabrication of a Novel Culture Dish Adapter with a Small Recess Structure for Flow Control in a Closed Environment

2019 ◽  
Vol 9 (2) ◽  
pp. 269 ◽  
Author(s):  
Reiko Yasuda ◽  
Shungo Adachi ◽  
Atsuhito Okonogi ◽  
Youhei Anzai ◽  
Tadataka Kamiyama ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Proliferation ◽  
Flow Control ◽  
Liquid Flow ◽  
Culture Medium ◽  
Control Cell ◽  
Culture Dish ◽  
Flow Area ◽  
Waste Products ◽  
Medium Flow

Cell culture medium replacement is necessary to replenish nutrients and remove waste products, and perfusion and batch media exchange methods are available. The former can establish an environment similar to that in vivo, and microfluidic devices are frequently used. However, these methods are hampered by incompatibility with commercially available circular culture dishes and the difficulty in controlling liquid flow. Here, we fabricated a culture dish adapter using polydimethylsiloxane that has a small recess structure for flow control compatible with commercially available culture dishes. We designed U-shaped and I-shaped recess structure adapters and we examined the effects of groove structure on medium flow using simulation. We found that the U-shaped and I-shaped structures allowed a uniform and uneven flow of medium, respectively. We then applied these adaptors to 293T cell culture and examined the effects of recess structures on cell proliferation. As expected, cell proliferation was similar in each area of a dish in the U-shaped structure adapter, whereas in the early flow area in the I-shaped structure adapter, it was significantly higher. In summary, we succeeded in controlling liquid flow in culture dishes with the fabricated adapter, as well as in applying the modulation of culture medium flow to control cell culture.

scholarly journals The Effect of miR-138 on the Function of Follicular Helper T Cells and the Differentiation of B Cells in Osteosarcoma

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Zhitao Wang ◽  
Jianxiao Liang ◽  
Shanyong Jiang ◽  
Gang Zhao ◽  
Jianshu Lu ◽  
...  
Keyword(s):  
Cell Culture ◽  
Flow Cytometry ◽  
B Cells ◽  
Culture Medium ◽  
Membrane Surface ◽  
Surface Antigens ◽  
Cell Culture Medium ◽  
Tfh Cells ◽  
Follicular Helper ◽  
Cell Membrane Surface

Objective. To explore the effect of miR-138 on the function of follicular helper T (Tfh) cells and the differentiation of B cells in osteosarcoma. Methods. Clinically collect peripheral blood from osteosarcoma (OS) patients and healthy volunteers (HC), as well as OS tumor tissues (OS tumor) and adjacent tissues with normal histology (normal group). The CD4+CXCR5+Tfh cells of OS patients were screened and isolated by flow cytometry, and the expression of Tfh cell membrane surface antigens PD-1 and CTLA-4 was detected. In addition, qRT-PCR was used to detect the expression of miR-138 in tissues and Tfh cells, and the correlation relationship between miR-138 and PD-1 and CTLA-4 was analyzed. After interference or overexpression of miR-138 in Tfh cells, coculture with untreated B cells was done, and the levels of IL-10, IL-12, IL-21, and INF-γ in Tfh cell culture medium and the levels of IgM, IgG, and IgA in B cell culture medium after coculture were measured by ELISA. Flow cytometry was used to detect the expression of B cell membrane surface antigens CD27 and CD38 after coculture. Results. The rate of PD-1- and CTLA-4 positive cells in the peripheral blood and tissues of the OS group was significantly increased, the expression of miR-138 was significantly reduced, and the expression of miR-138 was negatively correlated with the expression of PD-1 and CTLA-4. In addition, upregulation of miR-138 can lead to a significant increase in the level of IL-10 in the supernatant of Tfh cells and a significant decrease in the levels of IL-12, IL-21, and INF-γ, which in turn leads to increased levels of IgM, IgG, and IgA released by B cells. At the same time, it significantly increases the rate of CD27- and CD38-positive cells and promotes the maturation of B cells. Downregulating miR-138 has the opposite effect. Conclusion. Downregulating the expression of miR-138 in osteosarcoma can improve the dysfunction of CD4+CXCR5+Tfh cells and promote the differentiation of B cells.

scholarly journals Effects of Environmental pH on the Growth of Gastric Cancer Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Wenjie Li ◽  
Ying Zhou ◽  
Chunyu Shang ◽  
Hui Sang ◽  
Hong Zhu
Keyword(s):  
Gastric Cancer ◽  
Cell Culture ◽  
Flow Cytometry ◽  
Cancer Cells ◽  
Culture Medium ◽  
Ph Value ◽  
Acid Suppression ◽  
Biological Behavior ◽  
Cell Culture Medium ◽  
Gastric Cancer Cells

Background. Proton pump inhibitor (PPI) and other acid-suppressing drugs are widely used in the treatment of gastrointestinal ulcer, upper gastrointestinal bleeding, gastritis, and gastric cancer (GC). About 80% of GC patients receive acid suppression treatment. PPI suppresses the production of gastric acid by inhibiting the function of H+/K+-ATPase in gastric parietal cells and raises the pH value to achieve therapeutic purposes. Some studies have found that PPI had a certain antitumor effect in the proliferation and apoptosis of tumor cells. But the effects of environmental pH on the growth of GC cells and its mechanism are unknown. Therefore, we hoped to find the effects of culture medium pH on the biological behavior of GC cells by in vitro experiments and provide guidance for the use of acid-suppressing drugs in GC patients. Aims. We aimed to observe the effects of pH changes in GC cell culture medium on the cell biological behavior of cancer cells and to analyze the potential mechanisms. We hoped to find out the effect of acid suppression on the growth of GC cells. Methods. The GC cell lines (SGC-7901 and MKN45) were used as the research object. We adjusted the pH value in the cell culture medium to observe the changes in cell viability (MTT), apoptosis (flow cytometry), and invasion (Transwell) at pH 6, pH 7, and pH 8. qRT-PCR and western blot (WB) assays were used to determine the expression changes of genes and proteins (mTOR, AKT, Wnt, Glut, and HIF-1α) at pH 6, pH 7, and pH 8. Results. The results of MTT showed that the viability of SGC-7901 and MKN45 in the pH 8.0 group was significantly weaker than that in the pH 6.0 or pH 7.0 group (P<0.001). Flow cytometry results showed that the apoptosis of SGC-7901 and MKN45 in the pH 8.0 group was more obvious than that in the pH 6.0 or pH 7.0 group (P<0.001). The results of Transwell showed that the invasion ability of SGC-7901 and MKN45 in the pH 8.0 group was significantly weaker than that in the pH 6.0 or pH 7.0 group (P<0.001). As shown by PCR and WB results, with the increase of pH, the expression of mTOR, AKT, Wnt, Glut, and HIF-1α in SGC-7901 and MKN45 was downregulated (P<0.05). Conclusions. Compared with the microacid environment, the microalkaline environment inhibited the viability, invasion, and expression of genes and proteins (mTOR, AKT, Wnt, Glut, and HIF-1α) but promoted the apoptosis of GC cells and thus inhibited the growth of GC.

3-iodothyronamine (T1AM) is taken up and rapidly metabolized in cell culture medium only at low concentration

2020 ◽  
Author(s):  
Federica Saponaro ◽  
Marco Borsò ◽  
Sara Verlotta ◽  
Lavinia Bandini ◽  
Alessandro Saba ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Low Concentration

Effect of Atmospheric-Pressure Helium Plasma Jet on Cell Culture Medium

2013 ◽  
Vol 133 (5) ◽  
pp. 278-285
Author(s):  
Norimitsu Takamura ◽  
Douyan Wang ◽  
Takao Satoh ◽  
Takao Namihira ◽  
Hisato Saitoh ◽  
...  
Keyword(s):  
Cell Culture ◽  
Atmospheric Pressure ◽  
Culture Medium ◽  
Plasma Jet ◽  
Helium Plasma ◽  
Cell Culture Medium

Analysis of efficiency of application of media with hyaluronic acid in cryoprotocols

GYNECOLOGY ◽  
2020 ◽  
Vol 22 (2) ◽  
pp. 26-29
Author(s):  
Natalia V. Protopopova ◽  
Elena B. Druzhinina ◽  
Kseniia V. Krylova ◽  
Iuliia V. Mylnikova ◽  
Jan A. Dvoryanov ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Culture Medium ◽  
Genetic Diagnosis ◽  
World Health ◽  
Practical Significance ◽  
Pelvic Surgery ◽  
High Concentration ◽  
Tubal Infertility

According to the World Health Organization, about 2 million new couples experience infertility annually, and their number is growing. An effective way to overcome infertility is assisted reproductive technology (ART). Cryopreservation will rationally solve the issue of preservation and further use of embryos: to delay pregnancy for some time considering womans desire and to prevent ovarian hyperstimulation syndrome. Embryo freezing allows to reduce the rate of repeated ovarian stimulation and perform preimplantation genetic diagnosis. Over the past decades, various cryotransfer options have been proposed to increase ART treatment efficacy, including the use of a culture medium with a high concentration of hyaluronic acid, but there are conflicting data on the use of such a medium in ART programs. Aim. Evaluation of thawed embryo transfers efficacy using the hyaluronic acid-containing culture medium. To achieve the goal, the following tasks were set: to evaluate clinical and medical history data of patients with tubal infertility in cryoprotocols, to analyze the previous cycle of in vitro fertilization and embryological stage, to evaluate the effectiveness of the culture medium with a high content of hyaluronic acid. Materials and methods. A detailed description of the patient sample, inclusion and exclusion criteria, embryological stage, embryo grading, devitrified embryo transfer technique. The article includes 3 tables which present the groups general clinical characteristics, the embryological stage, the rate of pregnancy, depending on the cultivation day. Results. The authors established that in patients with a history of pelvic surgery and sexually transmitted infections, it is advisable to use the culture medium with a high content of hyaluronic acid to transfer the thawed embryo. It was shown that pregnancy rate is 1.5 times higher when transferring devitrified embryos on the 5th day of development with the use of hyaluronic acid-containing culture medium. The conclusion about the pregnancy rate in obese patients is not indisputable, which requires further study. The authors also provide practical recommendations on the use of the culture medium with hyaluronic acid in cryoprotocols. Conclusion. The study allows to optimize the devitrified embryo transfer in patients with tubal infertility using a culture medium with a high content of hyaluronan. This work has undoubted scientific and practical significance.

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