In-Silico Analysis of Interacting Pathways Through Kim-1 Protein Interaction in Diabetic Nephropathy:
Abstract Human Kidney Injury Molecule-1 also known as HAVCR-1 (Hepatitis A virus cellular receptor 1) belongs to cell-surface protein of immunoglobulin superfamily involved in phagocytosis by acting as scavenger receptor epithelial cells. The study focused to pinpoint the mechanisms and gene that interact with KIM-1.Methods: This in-silico study was done from March, 2019 to December 2019 .First the Enrichment and protein–protein interaction (PPI) network study of the carefully chosen proteins together with diagramed gene data sets was accomplished using FunRich version 3.1.3.it was done to unveil the proteins that may have an effect on the regulation of HAVCR1 or are may be regulated by this protein. These genes were then further considered in pathway analysis to discover the dysregulated pathways in diabetic nephropathy. The long list of differentially expressed genes is meaningless without pathway analysis. Results: Important pathways that are dysregulated in diabetic nephropathy patients are identified including Immune System (Total = 237, P<0.05), Innate Immune System (Total=140, P<0.05), Cytokine Signaling Immune system (Total = 116, P<0.05), Adaptive Immune System (Total =85) and Neutrophil degranulation (Total=78).Conclusion: The top 5 genes that are interacting directly with HIVCR1 include CASP3, CCL2, SPP1, B2M, and TIMP1 with degrees 161, 144, 108, 107, and 105 respectively for Immune system pathways (Innate Immune System, Cytokine Signaling Immune system, Adaptive Immune System and Neutrophil degranulation)