scholarly journals The determination of high retention flag marking methods with yellowfin bream (Acanthopagrus latus) fry

2021 ◽  
Author(s):  
Po-Cheng Chen ◽  
CHUN-HAN SHIH ◽  
Jia-Jiao Wang ◽  
Liang-Ming Huang
Keyword(s):  
Retention Rate ◽  
Hard Tissue ◽  
Fishery Resources ◽  
Live Fish ◽  
Acanthopagrus Latus

Abstract With the decline in coastal fishery resources, hatchery-reared fry of yellowfin bream (Acanthopagrus latus) have been commonly mass released to the surrounding waters in China in recent years. Although the release plan has been underway in China for many years, the releasing effects were not often assessed. Therefore, it is necessary to study several suitable mass marking methods that have a high mark retention rate for fry. From October to November 2020, tetracycline was immersed to mark calcified hard tissue (MI), with tagging cut tail fin (TC) and the control, and the species of yellowfin bream by live fish were examined. Moreover, a double marking method, i.e., mark calcified hard tissue (MI) after tag fish for two weeks, was examined to determine any increase in the mark retention rate for yellowfin bream in November 2020. The dual marking and tagging method combining MI and TC is effective and is suitable for yellowfin bream. Both MI of 0.25% treatment and MI of 0.5% treatment also have lower costs, and more individuals can be marked.

scholarly journals KONDISI DAN PRIORITAS UNTUK MENGENDALIKAN PEMANFAATAN IKAN NAPOLEON (Cheilinus undulatus, RÜPPELL, 1835) DI KABUPATEN RAJA AMPAT

2018 ◽  
Vol 23 (3) ◽  
pp. 181
Author(s):  
Hendrik Sombo ◽  
Mohammad Mukhlis Kamal ◽  
Yusli Wardiatno
Keyword(s):  
Analytical Hierarchy Process ◽  
Management Decisions ◽  
Ahp Model ◽  
Fish Resources ◽  
Resource Sustainability ◽  
Live Fish ◽  
Priority Order ◽  
Control And Management ◽  
Hierarchy Process

Hasil survei potensi yang dilakukan IUCN dan LPSPL Sorong menunjukkan nilai kelimpahan ikan napoleon (Cheilinus undulatus) di Kabupaten Raja Ampat termasuk dalam kategori rendah. Walaupun demikian, nelayan tetap menginginkan ada kuota untuk pemanfaatannya karena merupakan salah satu sumber mata pencaharian utama. Aktivitas pemanfaatan harus disertai dengan upaya pengendalian untuk menjaga kelestarian sumberdaya. Penerapan upaya fishing control dan manajemen kebijakan belum dilakukan secara optimal, sehingga diperlukan penetapan berdasarkan skala prioritas. Tujuan penelitian ini adalah untuk mengidentifikasi kondisi pemanfaatan dan menentukan skala prioritas upaya untuk mengendalikan pemanfaatan ikan napoleon di Kabupaten Raja Ampat. Penentuan skala prioritas dianalisis dengan model Analytical Hierarchy Process (AHP) dengan melibatkan pakar di bidang konservasi dalam penilaian. Berdasarkan hasil identifikasi, kondisi pemanfaatan ikan napoleon di Kabupaten Raja Ampat dilakukan belum sesuai dengan ketentuan regulasi yang telah ditetapkan. Hasil penilaian dengan model AHP menunjukkan bahwa kriteria yang paling penting untuk mengendalikan pemanfaatan ikan napoleon adalah sumberdaya ikan lestari, dan urutan prioritas alternatif adalah sosialisasi kepada nelayan/pengusaha (bobot 0,2935), pengawasan (bobot 0,2715), survei kelimpahan secara berkala (bobot 0,2181) dan penerapan lalu lintas perdagangan melalui bandara (bobot 0,2169).The results of potential survey by IUCN and LPSPL Sorong showed the abundance value of napoleon fish (Cheilinus undulatus) in Raja Ampat Regency was low category. Nonetheless, the fisher quota for its utilization because it is one of the main sources of livelihood. The utilization activities should be followed by control of effort to maintain resource sustainability. Implementation of fishing control and management decisions has not been done optimally, therefore it need to be set based on priority scale. The purpose of this study were to identify conditions of utilization and to determine the priority scale to control utilization of napoleon wrasse fishery in Raja Ampat Regency. Determination of priority scale were analyzed with Analytical Hierarchy Process (AHP) model by involving experts in the field of conservation in the assessment. Based on the results of identification, the condition of the utilization of napoleon fish in Raja Ampat Regency was not with the regulation. The results of the assessment with AHP model showed that the most important criteria to control utilization of napoleon wrasse fishery is sustainable fish resources, and alternatives priority order are socialization/awareness program to fishers/live fish buyer (value 0.2935), surveillance (value 0.2715), periodic density surveys (value 0.2181) and implementation of trade air-only (value 0.2169).

Determination of downs hard tissue cephalometric norms for Himachali Mongoloid tribes

2019 ◽  
Vol 78 (1) ◽  
pp. 11-17
Author(s):  
Sanjeev Vaid ◽  
Santosh Verma ◽  
K.S. Negi ◽  
J.R Kaundal ◽  
Sankalp Sood ◽  
...  
Keyword(s):  
Hard Tissue

Hard-tissue Substrates for Evaluation of Cariogenic and Anti-cariogenic Activity in situ

1992 ◽  
Vol 71 (3_suppl) ◽  
pp. 913-919 ◽  
Author(s):  
J.R. Mellberg
Keyword(s):  
Root Surface ◽  
Hard Tissue ◽  
Suitable Alternative ◽  
Human Enamel ◽  
Human Dentin ◽  
Bovine Enamel ◽  
Root Surface Caries

Hard-tissue substrates include primarily human and bovine enamel and human dentin. They have been used for in situ studies in a natural or sound condition, as well as flattened or containing an in vitro-formed caries-like lesion. Human enamel and dentin are generally the substrates of choice for studies of coronal and root-surface caries, respectively, but bovine enamel appears to offer a suitable alternative for many studies of enamel caries. Substrates with caries-like lesions will respond more rapidly to changes in the intra-oral mineral equilibrium and will allow both demineralization and remineralization to be determined. Findings from some studies suggest that caries-like lesions may respond somewhat differently, depending upon the degree of mineralization of the surface layer. Because in vitro findings with dentin show it to be significantly more soluble in acid than enamel, results from a study that used dentin may not be directly applicable to enamel. Both enamel and dentin substrates can be used in thin-section models. Hard-tissue substrates can also differ, depending upon their intra-oral location. Locations that result in the accumulation of plaque will behave differently from those that are plaque-free. So that plaque would accumulate, substrates have been placed approximally, beneath a fabric or steel mesh, in a protected trough, beneath a metal band or within a depression on the buccal surface. For studies requiring a determination of both demineralization and remineralization, human enamel or dentin containing a surface-softened caries-like lesion and covered with a uniform natural plaque are the substrates of choice.

scholarly journals Non-invasive ploidy determination in live fish by measuring erythrocyte size in capillaries

2021 ◽  
Vol 51 (3) ◽  
pp. 275-280
Author(s):  
Karel Halačka ◽  
Karel Janko ◽  
Lukáš Vetešník
Keyword(s):  
Flow Cytometry ◽  
Rapid Determination ◽  
Minimum Size ◽  
Gibel Carp ◽  
Small Fish ◽  
Reliable Determination ◽  
Live Fish ◽  
Blood Smears ◽  
The Individual

Information about ploidy is important in both commercial and conservation aquaculture and fish research. Unfortunately, methods for its determination, such as karyology, determination of the amount of DNA in a cell using microdensitometry or flow cytometry and/or measuring erythrocytes in a blood smear can be stressful or even destructive. Some of these methods are also limited by the relatively large minimum size of the individual being measured. The aim of this study was to test a new low-stress method of determining ploidy by measuring the size of erythrocytes in the capillaries of a fish, including small individuals. First, we examined diploid and triploid loach (Cobitis sp.) and gibel carp, Carassius gibelio (Bloch, 1782), using flow cytometry and blood smears, with these results being used as a control. Subsequently, we measured the size of erythrocytes in the caudal fin capillaries of anesthetized fishes of known ploidy under a light microscope. For both the loaches and gibel carp, direct observation of the mean erythrocyte size in epithelial fin capillaries provided a consistent and reliable determination of ploidy when compared with the controls based on flow cytometry and blood smears. This new method allows for rapid determination of ploidy in living small fish, where collection of tissue using other methods may cause excessive stress or damage. The method outlined here simply requires the measurement of erythrocytes directly in the bloodstream of a live fish, thereby making it possible to determine ploidy without the need for blood sampling. The method described is sufficiently efficient, less demanding on equipment than many other procedures, can be used by relatively inexperienced personnel and has benefits as regards animal welfare, which is especially important for fish production facilities or when dealing with rare or endangered species.

scholarly journals Clinical And Laboratory Results Of Intrauterine Device Cu T 380 A Intrauterine System Intraoperative Injection

2020 ◽  
Vol 2 (09) ◽  
pp. 89-95
Author(s):  
Nasirova Zebiniso Azizovna ◽  
Negmadjanov Bahodur Boltaevich
Keyword(s):  
Caesarean Section ◽  
Clinical Outcome ◽  
Retention Rate ◽  
Intrauterine Device ◽  
Maternity Hospital ◽  
Contraceptive Methods ◽  
Unplanned Pregnancies ◽  
Laboratory Results ◽  
Post Placental

Effective and safe contraceptive methods not only prevent unplanned pregnancies and their termination, but also make it possible to fully restore the mother's body after surgery to carry out the necessary pre-pregnancy preparation. The goal of the study was to evaluate the clinical outcome of postplacental administration of Cu T 380 A in women during a caesarean section. The study was conducted at Samarkand Maternity Hospital No. 2 to assess the clinical outcome of postplacental administration of Cu T 380 A in women during caesarean section. The present study showed that there were no serious complications and only minor side effects such as pain, fever, bleeding and irregular bleeding, which were observed only in some women during the hospital stay and the control visit in the first 6 months. The determination of vaginal BMW tendrils was made possible in 72.12% of women during the control visit after 6 weeks. The visibility of the tendrils increased over time and after 6 months they were seen in 90.81% of cases. Exposure frequency was 2 cases (4%) and another 2 women (4%) required removal of the PMTCT for various reasons, resulting in a 92.3% retention rate six months after contraception. No cases of pregnancy were registered. Post-placental introduction of BIC Cu T 380 A during a C-section is a practical, convenient, safe, effective and acceptable contraceptive method. 

scholarly journals Quantification of Bacterial Colonization in Dental Hard Tissues Using Optimized Molecular Biological Methods

2020 ◽  
Vol 11 ◽  
Author(s):  
Torsten Sterzenbach ◽  
Anne Pioch ◽  
Martin Dannemann ◽  
Christian Hannig ◽  
Marie-Theres Weber
Keyword(s):  
Biofilm Formation ◽  
Bacterial Colonization ◽  
Hard Tissue ◽  
Bacterial Dna ◽  
Root Canals ◽  
Dental Hard Tissue ◽  
Dental Hard Tissues ◽  
Hard Tissues ◽  
Biological Methods

Bacterial infections of root canals and the surrounding dental hard tissue are still a challenge due to biofilm formation as well as the complex root canal anatomy. However, current methods for analyzing biofilm formation, bacterial colonization of root canals and dental hard tissue [e.g., scanning electron microscopy, confocal laser scanning microscopy (CLSM) or determination of colony forming units (CFU)] are time-consuming and only offer a selective qualitative or semi-quantitative analysis. The aim of the present study is the establishment of optimized molecular biological methods for DNA-isolation and quantification of bacterial colonization via quantitative PCR (qPCR) from dental hard tissue. Root canals of human premolars were colonized with Enterococcus faecalis. For isolation of DNA, teeth were then grinded with a cryo mill. Since the hard tissues dentin and especially enamel belong to the hardest materials in the human organism, the isolation of bacterial DNA from root dentin is very challenging. Therefore, treatment steps for the isolation of DNA from grinded teeth were systematically analyzed to allow improved recovery of bacterial DNA from dental hard tissues. Starting with the disintegration of the peptidoglycan-layer of bacterial cells, different lysozyme solutions were tested for efficacy. Furthermore, incubation times and concentrations of chelating agents such as EDTA were optimized. These solutions are crucial for the disintegration of teeth and hence improve the accessibility of bacterial DNA. The final step was the determination of prior bacterial colonization of each root canal as determined by qPCR and comparing the results to alternative methods such as CFU. As a result of this study, optimized procedures for bacterial DNA-isolation from teeth were established, which result in an increased recovery rate of bacterial DNA. This method allows a non-selective and straightforward procedure to quantify bacterial colonization from dental hard tissue. It can be easily adapted for other study types such as microbiome studies and for comparable tissues like bones.

Preparation and Characterization of Broad-Spectrum Sunscreens Loaded Nanostructured Lipid Carrier

2013 ◽  
Vol 669 ◽  
pp. 67-71
Author(s):  
Yan Ling Chen ◽  
Qiang Xia
Keyword(s):  
Particle Size ◽  
Physical State ◽  
Retention Rate ◽  
Nanostructured Lipid Carrier ◽  
High Pressure Homogenization ◽  
Spectrophotometric Detection ◽  
Ultraviolet Absorbance ◽  
Organic Uv Filters

A comprehensive photoprotective formulation was developed to enhance photoprotection ,compared to a conventional nanoemulsion ,having the concentration of two new molecular sunscreens, namely Diethylamino hydyoxybenzoyl hexyl benzoate(Uvinul ® A Plus) and Ethylhexyl triazone(Uvinul® T 150). In the present paper, the sunscreen mixture was incorporated into nanostructured lipid carriers (NLCs). After the production by hot high pressure homogenization, the NLC were analyzed in terms of particle size, physical state, particle shape, ultraviolet absorbance and stability. The particle size for all NLC was around 100-900 nm after production. The determination of 2 organic UV filters was performed by HPLC with UV spectrophotometric detection. The loading capacities for molecular sunscreens was up to 30%. In the sunlight, the retention rate of Uvinul ® A Plus and Uvinul ® T150 was still up to 80% after three months .

Indicator’s Weight Determination of Evaluating Reclamation’s Impact on Marine Resources

2012 ◽  
Vol 610-613 ◽  
pp. 900-903
Author(s):  
Cong Hu ◽  
Ding Yong Yu
Keyword(s):  
Delphi Method ◽  
Coastal Wetland ◽  
Marine Resources ◽  
Reliability Test ◽  
Test Results ◽  
Land Area ◽  
Statistical Software ◽  
Fishery Resources ◽  
Expert Opinions

Although reclamation can increase land area, it decreases natural shoreline, coastal wetland and fishery resources. It remains a contradiction between the utilization of reclamation and protection of marine resources. The effective method to judge reclamation impact on marine resources is of great importance. Based on results of three rounds expert investigation, this paper established a system to make the judgement. Two steps were taken: using Delphi method to obtain the weight of qualitative and quantitive evaluation indicators, using SPSS statistical software to get reliability test results, which show that expert opinions are in well agreement. The indicator weights were finally gived.

scholarly journals Molecular Recognition at the Protein-Hydroxyapatite Interface

2003 ◽  
Vol 14 (5) ◽  
pp. 370-376 ◽  
Author(s):  
Patrick S. Stayton ◽  
Gary P. Drobny ◽  
Wendy J. Shaw ◽  
Joanna R. Long ◽  
Michele Gilbert
Keyword(s):  
Stone Formation ◽  
Arterial Calcification ◽  
Fusion Peptides ◽  
Hard Tissue ◽  
Recognition Mechanism ◽  
Biologically Relevant ◽  
Pathway Activation ◽  
Mineralized Tissues ◽  
Hard Tissue Engineering

Proteins found in mineralized tissues act as nature’s crystal engineers, where they play a key role in promoting or inhibiting the growth of minerals such as hydroxyapatite (bones/teeth) and calcium oxalate (kidney stones). Despite their importance in hard-tissue formation and remodeling, and in pathological processes such as stone formation and arterial calcification, there is little known of the protein structure-function relationships that govern hard-tissue engineering. Here we review early studies that have utilized solid-state NMR (ssNMR) techniques to provide in situ secondary-structure determination of statherin and statherin peptides on their biologically relevant hydroxyapatite (HAP) surfaces. In addition to direct structural study, molecular dynamics studies have provided considerable insight into the protein-binding footprint on hydroxyapatite. The molecular insight provided by these studies has also led to the design of biomimetic fusion peptides that utilize nature’s crystal-recognition mechanism to display accessible and dynamic bioactive sequences from the HAP surface. These peptides selectively engage adhesion receptors and direct specific outside-in signaling pathway activation in osteoblast-like cells.

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