scholarly journals Bokashi leachate as a biopriming on Basella rubra L. seed germination and root development

Author(s):  
Phooi Chooi Lin ◽  
Elisa Azura Azman ◽  
Roslan Ismail

Abstract Basella rubra L. is a type of spinach, which is edible with high nutrient composition. It is also known to be an antioxidant. However, initial germination and root growth remain an issue due to hard exterior seed coating. Thus, some may germinate within 10 to 21 days, and some may not work at all. Inhibited growth may lead to vegetative propagation and micropropagation, which fundamentally reduce the growth and yield. Basella seed treated with Bokashi leachate was found to improve seed germination and root growth. A study was conducted using food waste EM Bokashi leachate (0:1, 1:1500, 1:1000, 1:500) with biopriming duration (6 and 12 hours). The experiment was conducted in a completely random design (CRD) with 3 replications of 100 seeds, with 24 experimental units. Based on the results, a short biopriming duration (6 hours) significantly enhanced the mean germination rate, germination speed accumulated, and coefficient of germination velocity. However, germination percentage had no significant improvement by leachate. Long priming duration significantly reduced the root development due to the seed may loss of desiccation tolerance. The concentration of leachate and priming duration had no significant interaction. In order to improve the germination and root growth performance, 6 hours of seeds priming duration or 1:500 (0.2%) of food waste Bokashi leachate was recommended to soak the Basella rubra seeds.

1996 ◽  
Vol 121 (3) ◽  
pp. 423-429 ◽  
Author(s):  
Lewis W. Jett ◽  
Gregory E. Welbaum ◽  
Ronald D. Morse

Priming, a controlled-hydration treatment followed by redrying, improves the germination and emergence of seeds from many species. We compared osmotic and matric priming to determine which was the most effective treatment for improving broccoli seed germination and to gain a greater understanding of how seed vigor is enhanced by priming. Broccoli (Brassica oleracea L. var. italica) seeds were osmotically primed in polyethylene glycol (PEG 8000) at -1.1 MPa or matrically primed in a ratio of 1.0 g seed:0.8 g synthetic calcium silicate (Micro-Cel E):1.8 ml water at -1.2 MPa. In the laboratory, germination rates and root lengths were recorded from 5 to 42C and 10 to 35C, respectively. Broccoli seeds germinated poorly at >35C. Root growth after germination was more sensitive to temperatures >30C and <15C than radicle emergence. Matric and osmotic priming increased germination rate in the laboratory, greenhouse, and field. However, matric priming had a greater effect on germination and root growth rates from 15 to 30C. Neither priming treatment affected minimum or maximum germination or root growth temperatures. Both priming treatments decreased the mean thermal time for germination by >35%. The greater germination performance of matrically primed seeds was most likely the result of increased oxygen availability during priming, increased seed Ca content, or improved membrane integrity.


2014 ◽  
Vol 94 (4) ◽  
pp. 723-726
Author(s):  
D. J. Thompson

Thompson, D. J. 2014. Relating germination requirements of timber milkvetch to seral stage in the Interior Douglas-fir zone. Can. J. Plant Sci. 94: 723–726. Germination of timber milkvetch (Astragalus miser var. serotinus) seeds was studied to determine if it is an early or late seral species. Seeds were collected from a native plant population and those having a dark seed coat were selected. Germination tests were run in the light or dark and under three temperature regimes: low (constant 10°C), high (constant 25°C), and alternating (25°C day and 10°C night). Germination occurred over a protracted period with new germinants up to 90 d at the lowest temperature. Germination was not affected by light. Germination rate was greater at 25°C than 10°C, while fluctuating temperatures (25 and 10°C alternating every 12 h) reduced germination rate to that at the lower temperature. Days to 50% germination was a more sensitive indicator, with the mean for the fluctuating temperatures falling between those continuous to the high and low temperature. Final germination percentage was reduced at 10°C compared with 25°C, but not with fluctuating temperatures. Timber milkvetch seed germination did not respond to light or fluctuating temperatures, adding to a body of evidence that it is a late seral species.


2016 ◽  
Vol 74 (1) ◽  
pp. 85-92
Author(s):  
Mohammad Eteghadi Pour ◽  
Mahsa Hobbi ◽  
Hadi Ghasemi ◽  
Meisam Nazari

Abstract To study the effect of sonication on the seed germination percentage and rate as well as the cell area of barley (Hordeum vulgare L.) a laboratorial experiment was performed as Completely Randomized Design (CRD) with 3 replications. The results indicated that the ultrasonic waves affect the seed germination, germination rate and cell area significantly (at 0.05). The results of the mean comparison tests (LSD, 0.05) showed that the highest germination percentage (100 %), germination rate and cell area (1370.71 μ2) is achieved through 15 minutes exposure to ultrasonic waves. It is concluded that weakening the seed’s cell wall rigidity by sonication results in more and faster water imbibition by the cells and improved germination.


HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 577e-577 ◽  
Author(s):  
Monica Ozores-Hampton ◽  
Thomas A. Bewick ◽  
Peter Stoffella ◽  
Daniel J. Cantliffe ◽  
Thomas A. Obreza

The influence of compost (derived from MSW and biosolids) maturity on seed germination of several weed species was evaluated. A bioassay was developed by extracting 20 g of compost of different maturities with various volumes of water, then measuring germination percentage of ivyleaf morningglory (Ipomoea hederacea) seeds placed on extract-saturated filter paper in a petri dish. A 20 g (dry weight) compost: 50 mL of water generated an extract that produced the widest percentage seed germination variation in response to composts of different maturity. Ivyleaf morningglory, barnyardgrass (Echinochloa crus-galli L.), purslane (Potulaca oleracea L.), and corn (Zea mays L) were selected as plant indicators to determine the compost maturity stage with maximum germination inhibition. Compost 8-week-old decreased percent germination, root growth, and germination index (combines germination rate and root growth), and increased mean days to germination (MDG) of each plant indicator. Immature 8 week-old compost extract effect on MDG and germination percent of 15 weed species was evaluated. Extract from 8-week-old compost inhibited germination in most weed species, except yellow nutsedge (Cyperus esculentus). Compost extracts derided from immature (3-day, 4-, and 8-week-old) compost resulted in delayed and reduced germination percent of important economic weed species.


1975 ◽  
Vol 5 (3) ◽  
pp. 419-423 ◽  
Author(s):  
Carey Borno ◽  
Iain E. P. Taylor

Stratified, imbibed Douglas fir (Pseudotsugamenziesii (Mirb.) Franco) seeds were exposed to 100% ethylene for times between 0 and 366 h. Germination rate and germination percentage were increased by treatments up to 48 h. The 12-h treatment gave largest stimulation; 30% enhancement of final germination percentage over control. Treatment for 96 h caused increased germination rate for the first 5 days but reduced the germination percentage. Germinants were subject to continuous exposure to atmospheres containing 0.1 – 200 000 ppm ethylene in air, but it did not stimulate growth, and the gas was inhibitory above 100 ppm. Although some effects of high concentrations of ethylene may have been due to the lowering of oxygen supplies, this alone was insufficient to account for the full inhibitory effect. The mechanism of stimulation by short-term exposure to ethylene is discussed.


2021 ◽  
Vol 43 ◽  
Author(s):  
Nancy Araceli Godínez-Garrido ◽  
Juan Gabriel Ramírez-Pimentel ◽  
Jorge Covarrubias-Prieto ◽  
Francisco Cervantes-Ortiz ◽  
Artemio Pérez-López ◽  
...  

Abstract: Chitosan is a biopolymer obtained from deacetylation of chitin; it has multiple applications in agriculture as an antifungal, soil conditioner, inducer of defense mechanisms, fruits postharvest coating, leaves and seeds, among others. The objective in this research was to evaluate the effect of chitosan coatings mixed with fungicide (dithiocarbamate) on the germination and germination speed of bean and maize seeds in storage and to determine the retention capacity of the fungicide in the coated seeds under different times of imbibition. Two coating treatments at concentrations of 0.1 and 0.5% chitosan in water, two coatings treatments at 0.1 and 0.5% chitosan supplemented with 0.5% fungicide and a coating without chitosan using only 0.5% fungicide in water were used in bean and maize seed; and as control seeds imbibed in distilled water were used; after treatments, germination percentage and germination speed were determined, also fungicide release were determined at 0, 1, 2 and 6 h of imbibition, and the effect of storage time on germination and germination speed was determined at 30, 60, 90, 120, 150 and 180 days of storage at 4 °C and 45% relative humidity. The fungicide release effect was determined by inhibiting Fusarium oxysporum conidia germination. There were no negative effects of coatings on seed germination after storage. The treatment that provided both greater retention of the fungicidal agent and released it gradually, was 0.5% chitosan mixed with fungicide concentration. Chitosan coating seeds mixed with fungicide do not cause negative changes in seed germination or germination rate.


2021 ◽  
Vol 1 (01) ◽  
pp. 27-30
Author(s):  
IRANI KHATUN ◽  
RIYAD HOSSEN

Seed germination performance test of Taherpuri onion (a local variety of onion) under six different temperatures (15, 20, 25, 30, 35 and 40°C) was the main goal of this experiment. Germination percentage (GP) was calculated at highest 60.25% at 25°C, and the highest germination rate 20.08 was observed in the same temperature condition. The lowest germination performance (13.25 % germi-nation and 3.32 seeds per day as germination rate) was found at 40°C temperature. Finally, the authors mentioned the temperature 20 to 30°C as optimum range, and suggested the temperature 25°C as best suited for obtaining highest results in case of both germination percentage and germination rate of these seeds. To produce maximum seedlings of the local variety of onion, the mentioned temperature should be followed by the local farmers.


2010 ◽  
Vol 46 (2) ◽  
pp. 231-242 ◽  
Author(s):  
S. J. GOUSSOUS ◽  
N. H. SAMARAH ◽  
A. M. ALQUDAH ◽  
M. O. OTHMAN

SUMMARYA laboratory experiment was conducted to determine the effect of ultrasound (US) treatment on seed germination of chickpea, wheat, pepper and watermelon. All tests were carried out at 40 kHz in a water bath ultrasonic device varying two factors, treatment duration (5, 10, 15, 30, 45 or 60 min) and germination temperature (15 or 20 °C). Parallel tests were run in which seeds were soaked in water without sonication in order to eliminate the effect of water from US test results. The effects of US on seed germination varied between crops and were more obvious on germination speed, expressed as germination rate index (GRI), rather than on germination percentage (GP). In particular, US treatment significantly increased the GRI of chickpeas, wheat and watermelon, resulting in a maximum increase of 133% (at 45 min), 95% (30 min) and 45% (5 min), respectively, above control seeds. The beneficial effects of US on the GRI of these crops were observed at both 15 and 20 °C, suggesting that US treatment offers a practical priming method to overcome the slow germination that may occur at low temperatures. Water-soaking treatment improved the GP of both chickpea and pepper seeds by 59 and 24%, respectively, compared to the control but neither water nor US had any positive effect on pepper GRI. Post-treatment measurement of moisture content of these seeds produced variable results depending on crop species and US treatment duration. Results of this research indicated that US treatment effectively enhanced speed of germination of chickpea, wheat and watermelon seeds. This increase in speed of germination may improve early field establishment of these crops in the semiarid Mediterranean region and thus needs further investigation. The US technique may also be very useful for plant propagators in nurseries to achieve fast seedling establishment of watermelon.


2013 ◽  
Vol 5 (3) ◽  
pp. 325-331 ◽  
Author(s):  
Reyhaneh AZIMI ◽  
Hassan FEIZI ◽  
Mohammad KHAJEH HOSSEINI

The goal of this study was to evaluate concentrations of nanosized TiO2 at 0, 5, 20, 40, 60 and 80 mg L-1 with bulk TiO2 for possible stimulatory effects on wheatgrass seed germination and early growth stage. After 14 days of seed incubation, germination percentage improved by 9% following exposure to 5 ppm nanosized TiO2 treatment comparing to control. Similar positive effects occurred in terms of germination value and mean daily germination. Application of bulk TiO2 particles in 80 ppm concentration greatly decreased the majority of studied traits. Therefore phytotoxicity effect observed on wheatgrass seedling by application of bulk TiO2 particles in 80 ppm concentration. Exposure of wheatgrass seeds to 5 ppm nanosized TiO2 and bulk and nanosized TiO2 at 60 ppm obtained the lowest mean germination time but higher concentrations did not improve mean germination time. In general, there was a positive response by wheatgrass seed to some concentrations of nanosized TiO2. Usage of nanoparicles in order to improve germination and establishment of range plant in adverse environments similar to rangeland could be possible.


2013 ◽  
Vol 55 (2) ◽  
pp. 75-80 ◽  
Author(s):  
Ertan Yildirim ◽  
Atilla Dursun ◽  
Metin A. Kumlay ◽  
Ísmail Güvenç

This research was conducted to determine the effects of two biostimulants (humic acid and biozyme) or three different salt (NaCl) concentrations at the temperature 10, 15, 20 and 25°C on parsley, leek, celery, tomato, onion, lettuce, basil, radish and garden cress seed germination. Two applications of both biostimulants increased seed germination of parsley, celery and leek at all temperature treatments. Germination rate decreased depending on high salt concentrations. At different salt and temperature levels garden cress was characterised by the highest germination percentage compared to other vegetable species.Interactions between NaCl concentrations and temperatures, as welI as biostimulants and temperatures were significant at p=0.001 in for all vegetable species except onion in NaCl concentrations and temperatures compared to that of the control.


Sign in / Sign up

Export Citation Format

Share Document