scholarly journals Detection of Kita-Kyushu Lung Cancer Antigen-1, a Cancer/testis Antigen, in the stomach closer to the cancerous condition.

2020 ◽  
Author(s):  
Takashi Fukuyama ◽  
Toshikazu Otsuka ◽  
Nobue Futawatari ◽  
Kumiko Tahara ◽  
Masaaki Watanabe ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Lung Cancer ◽  
Helicobacter Pylori ◽  
Cancer Testis Antigen ◽  
Cancer Antigen ◽  
Exact Test ◽  
Cancer Prediction ◽  
Precancerous Condition ◽  
Cancer Testis

Abstract Background: Kita-Kyushu lung cancer antigen-1 (KK-LC-1) is a cancer/testis antigen (CTA) and is an attractive target for immunotherapy. An earlier study from our group demonstrated frequent KK-LC-1 expression in gastric cancers (GC) and non-tumor sites of the stomach carrying a tumor. Additionally, there was a correlation to Helicobacter pylori (Hp) infection. Currently it remains unclear whether KK-LC-1 is expressed in stomachs without gastric cancer.Methods: In the present study, we investigated differences in KK-LC-1 gene expression at non-tumor sites of stomachs with or without a tumor from 118 GC patients and 115 non-GC patients. Fisher’s exact test was used for the statistical analyses.Results: Our results show that KK-LC-1 gene expression was detected in 77% of non-tumor sites in stomachs with a tumor. Such findings were significantly higher than in stomachs without a tumor (7%, P <0.0001). All patients with KK-LC-1 expression at non-tumor sites of their stomachs without tumors carried Hp.Conclusions: KK-LC-1 appears to be detected in the stomach’s precancerous condition, but not in an atrophic stomach with Hp. KK-LC-1 may be a useful marker for gastric cancer prediction.

scholarly journals Cancer/testis antigen, Kita-Kyushu lung cancer antigen-1 and ABCD stratification for diagnosing gastric cancers

2020 ◽  
Vol 26 (4) ◽  
pp. 424-432
Author(s):  
Akiko Shida ◽  
Takashi Fukuyama ◽  
Nobue Futawatari ◽  
Haruki Omiya ◽  
Yoshinobu Ichiki ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Testis Antigen ◽  
Cancer Antigen ◽  
Gastric Cancers ◽  
Cancer Testis

scholarly journals Correlation Between Expression of the Cancer/Testis Antigen KK-LC-1 and Helicobacter pylori Infection in Gastric Cancer

In Vivo ◽  
2017 ◽  
Vol 31 (3) ◽  
pp. 403-407 ◽  
Author(s):  
TAKASHI FUKUYAMA ◽  
NOBUE FUTAWATARI ◽  
YOSHINOBU ICHIKI ◽  
AKIKO SHIDA ◽  
TAIGA YAMAZAKI ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Helicobacter Pylori Infection ◽  
Cancer Testis Antigen ◽  
Cancer Testis

scholarly journals Characteristics of Helicobacter pylori infection in a National Referral Hospital in Bhutan

2020 ◽  
Vol 6 (1) ◽  
pp. 6-10
Author(s):  
Chhabi Lal Adhikari ◽  
Guru Prasad Dhakal ◽  
Nongluck Suwisith ◽  
Sonam Dargay ◽  
Krishna P Sharma
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Age Groups ◽  
Referral Hospital ◽  
Upper Gastrointestinal Endoscopy ◽  
Age Group ◽  
Exact Test ◽  
Significant Difference ◽  
H Pylori ◽  
National Referral Hospital

Introduction: Helicobacter pylori (H. pylori) is a bacterium causing chronic gastric infection and may cause gastric cancer. It was necessary to see the trend of infection, especially in symptomatic patients. This retrospective descriptive study was aimed to describe the characteristics of H. pylori infection in Bhutanese patients referred for an endoscopy to the National Referral Hospital, Thimphu. Methods: The sample of the study was randomized 380 medical records of the patients who underwent upper gastrointestinal endoscopy and Rapid Urea Test for symptomatic dyspepsia and peptic ulcer. Data was collected using a survey form designed by the researchers. Data analysis was done using descriptive statistics and either Chi-square or Fisher’s exact test. Results: The prevalence of H. pylori infection was very high (76.6%). The mean age of the infection was 42 with a range from 15 to 84 years. The highest prevalence of infection was observed in the age group 20-29 years (82.7%) and lowest in the oldest age group 70-84 years (66.7%). The analysis showed no significant difference in infection amongst age groups, gender, and endoscopic findings to the positive results at 5% significant level except for monthly prevalence (p<0.001). Gastritis was the commonest endoscopy finding (153/380) and gastro-duodenitis had the highest positivity rate (88.9%). Conclusion: The prevalence of infection was relatively high compared with previous studies. Young and middle-aged adults had a high prevalence and this group needs to be given priority for screening and eradication treatment considering limited resources to prevent associated gastric cancer in Bhutan.

scholarly journals Genetic Polymorphisms of CXCL8 (−251) Are Associated with the Susceptibility of Helicobacter pylori Infection Increased the Risk of Inflammation and Gastric Cancer in Thai Gastroduodenal Patients

2019 ◽  
Author(s):  
Wongwarut Boonyanugomol ◽  
Kamolchanok Rukseree ◽  
Worrarat Kongkasame ◽  
Prasit Palittapongarnpim ◽  
Seung-Chul Baik ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Cancer Progression ◽  
Chemokine Receptor ◽  
Gene Polymorphisms ◽  
Inflammatory Responses ◽  
Increased Risk ◽  
Cxc Chemokine ◽  
H Pylori

CXC Chemokine Ligand 8 (CXCL8) plays an important role in gastric inflammation and in the progression of gastric cancer induced by Helicobacter pylori (H. pylori) infection. The association of CXCL8, CXC Chemokine Receptor 1 (CXCR1), and CXC Chemokine Receptor 2 (CXCR2) polymorphisms with H. pylori infection and gastric cancer progression needs to be investigated in a population within an enigma area consisting of multiple ethnicities, such as Thailand. To analyze the relative risk of H. pylori infection and gastric cancer among Thai gastroduodenal patients, gene polymorphisms in CXCL8 (promoter region -251) and in CXCR1 and CXCR2 (receptors for CXCL8) were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and allele specific-PCR (AS-PCR). We also determined the presence of cytotoxin-associated gene A (cagA) in Thai patients with H. pylori infection. Correlation between the CXCL8 (-251) polymorphism and CXCL8 gene expression was evaluated by quantitative reverse transcriptase-PCR (qRT-PCR). We found a significant association between the T/A and A/A genotypes of CXCL8 (-251) with H. pylori infection. However, no significant correlation was found between the CXCR1 (+2607) and CXCR2 (+1208) gene polymorphisms with H. pylori infection among Thai gastroduodenal subjects. Within the H. pylori-infected group of Thai gastroduodenal patients, no significant differences in cagA were observed. In addition, the A/A genotype of CXCL8 (-251) significantly correlated with the risk of gastric cancer and correlated with higher CXCL8 gene expression levels in Thai gastroduodenal patients. These results suggest that CXCL8 (-251) polymorphisms are associated with H. pylori infection, an increased risk of stronger inflammatory responses, and gastric cancer in Thai gastroduodenal patients.  

scholarly journals HLA-A2-restricted CTL epitopes of a novel lung cancer-associated cancer testis antigen, cell division cycle associated 1, can induce tumor-reactive CTL

2008 ◽  
Vol 123 (11) ◽  
pp. 2616-2625 ◽  
Author(s):  
Michiko Harao ◽  
Shinya Hirata ◽  
Atsushi Irie ◽  
Satoru Senju ◽  
Tetsuya Nakatsura ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Division ◽  
Cell Division Cycle ◽  
Cancer Testis Antigen ◽  
Ctl Epitopes ◽  
Cancer Testis

scholarly journals Inhibitory Effect of β-Carotene on Helicobacter pylori-Induced TRAF Expression and Hyper-Proliferation in Gastric Epithelial Cells

Antioxidants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 637 ◽  
Author(s):  
Yongchae Park ◽  
Hanbit Lee ◽  
Joo Weon Lim ◽  
Hyeyoung Kim
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Epithelial Cells ◽  
Nadph Oxidase ◽  
Gastric Epithelial Cells ◽  
Gastric Cancer Cells ◽  
Ags Cells ◽  
H Pylori ◽  
Β Carotene

Helicobacter pylori infection causes the hyper-proliferation of gastric epithelial cells that leads to the development of gastric cancer. Overexpression of tumor necrosis factor receptor associated factor (TRAF) is shown in gastric cancer cells. The dietary antioxidant β-carotene has been shown to counter hyper-proliferation in H. pylori-infected gastric epithelial cells. The present study was carried out to examine the β-carotene mechanism of action. We first showed that H. pylori infection decreases cellular IκBα levels while increasing cell viability, NADPH oxidase activity, reactive oxygen species production, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation, and TRAF1 and TRAF2 gene expression, as well as protein–protein interaction in gastric epithelial AGS cells. We then demonstrated that pretreatment of cells with β-carotene significantly attenuates these effects. Our findings support the proposal that β-carotene has anti-cancer activity by reducing NADPH oxidase-mediated production of ROS, NF-κB activation and NF-κB-regulated TRAF1 and TRAF2 gene expression, and hyper-proliferation in AGS cells. We suggest that the consumption of β-carotene-enriched foods could decrease the incidence of H. pylori-associated gastric disorders.

scholarly journals Phylogeographic Origin of Helicobacter pylori Determines Host-Adaptive Responses upon Coculture with Gastric Epithelial Cells

2013 ◽  
Vol 81 (7) ◽  
pp. 2468-2477 ◽  
Author(s):  
Alexander Sheh ◽  
Rupesh Chaturvedi ◽  
D. Scott Merrell ◽  
Pelayo Correa ◽  
Keith T. Wilson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Epithelial Cells ◽  
Gastric Disease ◽  
Gastric Epithelial Cells ◽  
Gastric Cancer Risk ◽  
Content Type ◽  
H Pylori ◽  
Induced Apoptosis

ABSTRACTWhileHelicobacter pyloriinfects over 50% of the world's population, the mechanisms involved in the development of gastric disease are not fully understood. Bacterial, host, and environmental factors play a role in disease outcome. To investigate the role of bacterial factors inH. pyloripathogenesis, global gene expression of sixH. pyloriisolates was analyzed during coculture with gastric epithelial cells. Clustering analysis of six Colombian clinical isolates from a region with low gastric cancer risk and a region with high gastric cancer risk segregated strains based on their phylogeographic origin. One hundred forty-six genes had increased expression in European strains, while 350 genes had increased expression in African strains. Differential expression was observed in genes associated with motility, pathogenicity, and other adaptations to the host environment. European strains had greater expression of the virulence factorscagA,vacA, andbabBand were associated with increased gastric histologic lesions in patients. In AGS cells, European strains promoted significantly higher interleukin-8 (IL-8) expression than did African strains. African strains significantly induced apoptosis, whereas only one European strain significantly induced apoptosis. Our data suggest that gene expression profiles of clinical isolates can discriminate strains by phylogeographic origin and that these profiles are associated with changes in expression of the proinflammatory and protumorigenic cytokine IL-8 and levels of apoptosis in host epithelial cells. These findings support the hypothesis that bacterial factors determined by the phylogeographic origin ofH. pyloristrains may promote increased gastric disease.

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