Metabolite Comparison between Serum and Follicular Fluid of Dairy Cows with Inactive Ovaries Postpartum
Abstract Inactive ovaries (IO) is a kind of postpartum ovarian disease in dairy cows, which sometimes accounts for 50% of ovarian disease, which seriously affects the dairy cows’s reproductive efficiency. To explore the metabolic changes in the serum and follicular fluid of dairy cows with IO during lactation, in this study six estrus (E) cows and six IO cows at 50 to 55 days in milk were selected according to B ultrasonic detection and clinical manifestations. The differential metabolites in serum and follicular fluid between the E cows and IO cows were identified by ultra-high-pressure liquid chromatography quadrupole time-of-flight mass spectrometry, combined with multidimensional statistical methods. The result shows that dairy cows with IO were in a subclinical ketosis status (BHBA > 1.20 mmol/L), 14 differential metabolites in the serum of IO cows included 10 increased metabolites and 4 decreased metabolites, while 14 differential metabolites in the follicular fluid of IO cows included 8 increased metabolites and 6 decreased metabolites. These differential metabolites mainly involve 9 metabolic pathways. Among them, the common enrichment pathway of different metabolites in serum and follicular fluid are glycerophospholipid metabolism, pentose and glucuronate interconversions. In conclusion, there are significant differences in the differential metabolites and enrichment pathways between serum and follicular fluid of IO cows. It implies that there are the complex changes in blood metabolism and local follicular metabolism of IO cows with subclinical ketosis, which their interaction relationship needs to be further confirmed.