scholarly journals Association Analysis and Estimation of Breeding Values of Iranian Table Grapevine Germplasm by Integrating Pomological and Molecular Data

2021 ◽  
Author(s):  
Mitra Razi ◽  
Reza Darvishzadeh ◽  
Hamed Baneh ◽  
Hadi Alipour
Keyword(s):  
Association Analysis ◽  
Fruit Set ◽  
Molecular Data ◽  
Table Grape ◽  
Breeding Value ◽  
Molecular Profile ◽  
Economic Traits ◽  
Breeding Values ◽  
Cluster Length ◽  
Grape Cultivars

Abstract Genetic resources in each country are valuable assets for sustainable development. An accurate knowledge of genetic behavior and identification of genomic loci associated with important economic traits will help breeders to run efficiently their breeding programs. Fourteen important pomological traits were measured on 45 Iranian table grape cultivars during three successive years. Molecular profile of studied cultivars was prepared with 39 (Simple sequence Repeat) SSR primers pairs. Genetic structure analysis based on the SSR markers revealed two sub-populations (K = 2) in the association panel. In association analysis using mixed linear model seven loci were found to be significantly associated with the studied traits at 5% probability level. Breeding values were also estimated for the pomological traits using the best linear unbiased prediction (BLUP). “Saghal Solian”, “At Ouzum”, “Garmian”, “Rishbaba Qermez”, “Taifi”, “Shahroudi”, “Sahebi Qermez”, “Lal Qermez”, “Alhaghi”, “Sarghola”, “Chava Ga”, “Qzl Ouzum” and “Agh Shani” table grape cultivars showed high and positive breeding value for cluster length, width and weight. Whereas “Garmian”, “Rishbaba Qermez”, “Fakhri”, “Agh Shani”, “Lal Sefid” and “Shirazi” had positive and high breeding value for pollen germination, fruit set in open pollination and fruit set under controlled pollination. Finally, Sarghola” and “Qzl Ouzum” showed the positive and highest breeding value for berry weight, flesh weight, cluster length, cluster width and cluster weight. The cultivar with high and positive breeding value can be used as a good parent for breeding of traits in hybridization programs because they can better transfer the desirable characteristics to the progeny in each case.

Selection of parents and estimation of genetic parameters using BLUP and molecular methods for lentil (Lens culinaris Medik.) breeding program in Argentina

2019 ◽  
Vol 56 (1) ◽  
pp. 12-25
Author(s):  
Carolina Bermejo ◽  
Federico Cazzola ◽  
Fernando Maglia ◽  
Enrique Cointry
Keyword(s):  
Quantitative Traits ◽  
Genetic Parameters ◽  
Selection Process ◽  
Recombinant Inbred Lines ◽  
Correlation Coefficients ◽  
Cost Effective ◽  
Molecular Data ◽  
Breeding Value ◽  
Breeding Values ◽  
High Yields

AbstractThe most important objective of lentil breeding programs is to develop new genotypes that are genetically more productive. Besides, it is necessary that the varieties obtained have short flowering cycles to allow the later sowing of summer crops. Selection is based through phenotypic means; however, we argue it should be based on genetic or breeding values because quantitative traits are often influenced by environments and genotype–environment interactions. The objectives of this study were to: (i) identify genotypes with the highest merit; (ii) estimate genetic parameters to know the genetic control of morphological traits in macrosperma and microsperma lentil types using best linear unbiased prediction (BLUP). Twenty-five recombinant inbred lines (RILs) from six F4 families selected on the basis of precocity and high yields were tested in four environments for important quantitative traits. The analysis of variance showed significant differences between genotypes, environments, and genotype–environment interactions for all the traits. Seven macrosperma- and two microsperma-type RILs were selected. Based on average ranking from breeding values and molecular data obtained with sequence-related amplified polymorphism (SRAP), the same genotypes were selected. Genotypic coefficients of variation, heritability across and by environment, and genetic correlation coefficients using BLUP were obtained. According to our results BLUP could replace molecular analysis methods because the selection process was simpler, more cost-effective, and more accurate. The breeding value of parents would give a better ranking of their genetic value than would their phenotypic value; therefore, the selection efficiency would be enhanced and the genetic gain would be more predictable. The selected genotypes could become potential commercial varieties or be used as parental lines in future hybridization programs.

scholarly journals 9 A comparison of different genomic prediction methods in the Canadian Landrace swine population

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 10-10
Author(s):  
Siavash Salek Ardestani ◽  
Mohsen Jafarikia ◽  
Brian Sullivan ◽  
Mehdi Sargolzaei ◽  
Younes Miar
Keyword(s):  
Genomic Prediction ◽  
Performance Test ◽  
Average Daily Gain ◽  
Single Step ◽  
Breeding Value ◽  
Prediction Methods ◽  
Nucleotide Polymorphisms ◽  
Economic Traits ◽  
Genetic Progress ◽  
Breeding Values

Abstract Increasing the accuracy of breeding value prediction can lead to more profitability through accelerating genetic progress for economic traits. The objective of this study was to assess the predictive abilities and unbiasedness of best linear unbiased prediction (BLUP) and popular genomic prediction methods of BayesC, BayesC(π = 0.99), genomic BLUP (GBLUP), and single-step GBLUP (ssGBLUP). Genotypic information (50K and 60K) of 4,890 performance tested Landrace pigs before February 2019 and 471 validation Landrace pigs that both had phenotypic information on backfat thickness (BFT), average daily gain (ADG), and loin muscle depth (LMD) from two Canadian pig breeding companies (AlphaGene and Alliance Genetics Canada) were used. The de-regressed breeding values (DEBV) were employed in GBLUP and Bayesian methods. A total number of 48,580 single nucleotide polymorphisms remained after quality control and imputation steps. The prediction accuracies were calculated using the correlation between predicted breeding values before performance test and DEBVs after performance test. All employed genomic prediction methods showed higher prediction accuracies for BFT (50.80–52.68%), ADG (26.61–34.47%), and LMD (18.25–25.08%) compared to BLUP method (BFT = 28.54%, ADG = 16.41%, LMD = 17.15%). The highest prediction accuracies for BFT and ADG were obtained using ssGBLUP method, and for LMD it was obtained using BayesC(π = 0.99). The BayesC(π = 0.99) showed also the lowest prediction biases across the studied traits (+0.05 for BFT, 0.00 for AGD, and -0.10 for LMD). In conclusion, our results revealed the superiority of ssGBLUP (for BFT and ADG) and BayesC(π = 0.99) (for LMD) over other tested methods in this study. However, the prediction accuracies from the tested genomic prediction methods were not significantly different from each other. Thus, employing these methods can be helpful for accelerating the genetic improvement of BFT, ADG, and LMD in the moderate population size of Canadian Landrace.

Sweet cherry collection composition and breeding value of Melitopol experimental station of horticulture

2019 ◽  
pp. 108-120
Author(s):  
L.M. Tolstolik
Keyword(s):  
Fruit Quality ◽  
Gene Pool ◽  
Sweet Cherry ◽  
Breeding Value ◽  
Economic Traits ◽  
Experimental Station ◽  
Parent Stock ◽  
Western European ◽  
One Year ◽  
Morphogenic Process

Aim. To evaluate the composition and breeding potential of a gene poolcollection of sweet cherry varieties of M.F. SydorenkoMelitopolResearch Station of Fruit Growing of the Institute of Horticulture, NAAS of Ukraine. Result and Discussion.The results of studying the morphological, economic and biological features of sweet cherry (Cerasusavium L. Moench) varieties from the gene pool collection of MelitopolExperimental Station of Horticulturenamed after M.F. Sidorenko of the Institute of Horticulture of NAAS are presented. The history of the collection creation and the genealogy of modern Ukrainian varieties were analyzed. We found that they were the 1st and 2nd generations of traditional Western European varieties. Major sweet cherry varieties that had been most often used in developing modern Ukrainian assortment, were identified. Analysis of the collection showed that 111 accessions of 129 were varieties bred in Melitopol, 40 of which are officially registered.The other accessions are elite forms, which failed the state variety trials or are being tested according to the competitive design. Among the latter, promising forms (candidates for varieties and pre-breeding forms) were distinguished, as they are sources of valuable economic traits. It was determined that the morphogenic process had contributed to the diversity in fruit quality features, which enabled building up a trait collection of sweet cherries for 20 traits with 82 levels of their expression. However, morphogenesis almost did not affect the growth power, fructification nature, generative organs, fructification time and resistance to diseases, therefore there are no significant differences in these features between the collection accessions bred at Melitopol Experimental Station of Horticulture named after M.F. Sidorenko of the Institute of Horticulture of NAAS. Most of the varieties grown on Magalebian cherry plantlet have extensive crowns, bear fruit on "May bouquets" and, after one-year growth, are winter hardy, tolerant to drought, moderately and highly resistant to spur blight and leaf spot as well as self-infertile. On this parent stock, most of the varieties come into fruition after 5-6 years and quickly increase their yields. It was established that enrichment of the gene pool collection with valuable introduced accessions and their wide involvement in hybridization were currently the key objectives aimed at expansion of morphogenesis limits to obtain competitive varieties with high adaptability, productivity and marketability. Conclusions. Modern Ukrainian varieties are the 1st and 2nd generations of traditional Western European varieties. German variety Drogansgelbeknorpelkirsche (DroganaZhyoltaya) with its clone Napoleon Belaya and Russian-Ukrainian variety Valeriy Chkalov were the most actively used in their development. 86% of the gene pool collection of MelitopolExperimental Stationof Horticulture named after M.F. Sidorenkoof the Institute of Horticulture of NAAS are varieties bred in Melitopol differing mainly in ripening time and fruit quality. The collection contains released varieties, a number of sources of valuable traits and, as a tool for the effective implementation of modern breeding programs, requires enrichment with varieties of other eco-geographical groups.

Influence of Light on Fruit Set of French Hybrid Grapes

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 510f-511 ◽  
Author(s):  
D.C. Ferree ◽  
S.J. McArtney ◽  
D.M. Scurlock
Keyword(s):  
Light Intensity ◽  
Linear Relationship ◽  
Fruit Set ◽  
Light Level ◽  
Fruit Color ◽  
Soluble Solids ◽  
Hue Angle ◽  
Reduced Light ◽  
Negative Linear Relationship ◽  
Grape Cultivars

Four French–American hybrid grape cultivars grown in a greenhouse were subjected to 5 days of 80% shade at four different times around bloom. Fruit set of `Seyval' was reduced by shade imposed before, during, or immediately after bloom. `Vidal' and `Chambourcin' were less sensitive, with fruit set reduced only by shade at bloom. Shade had little effect on fruit set of `DeChaunac'. In a second study, `Chambourcin' vines were exposed to ambient, ambient plus supplemental lights, and 30%, 50%, or 80% shade for 5 weeks beginning just prior to bloom. Fruit set was positively related to light intensity. At harvest, soluble solids, pH, and hue angle had a negative linear relationship to light level. Fruit color developed earliest and most rapidly with the reduced light treatments applied at bloom. Cluster weight was positively related to light intensity.

scholarly journals Explore the Rare—Molecular Identification and Wine Evaluation of Two Autochthonous Greek Varieties: “Karnachalades” and “Bogialamades”

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1556
Author(s):  
Dimitrios Evangelos Miliordos ◽  
Georgios Merkouropoulos ◽  
Charikleia Kogkou ◽  
Spyridon Arseniou ◽  
Anastasios Alatzas ◽  
...  
Keyword(s):  
Molecular Data ◽  
Molecular Profiling ◽  
Scientific Data ◽  
Financial Impact ◽  
Molecular Profile ◽  
Red Wines ◽  
Grape Berries ◽  
Quality Aspects ◽  
Commercial Vineyard ◽  
Of Greece

Wines produced from autochthonous Vitis vinifera varieties have an essential financial impact on the national economy of Greece. However, scientific data regarding characteristics and quality aspects of these wines is extremely limited. The aim of the current study is to define the molecular profile and to describe chemical and sensory characteristics of the wines produced by two autochthonous red grapevine varieties—“Karnachalades” and “Bogialamades”—grown in the wider area of Soufli (Thrace, Greece). We used seven microsatellites to define the molecular profile of the two varieties, and then we compared their profile to similar molecular data from other autochthonous as well as international varieties. Grape berries were harvested at optimum technological maturity from a commercial vineyard for two consecutive vintages (2017–2018) and vilification was performed using a common vinification protocol: the 2017 vintage provided wines, from both varieties, with greater rates of phenolics and anthocyanins than 2018, whereas regarding the sensory analysis, “Bogialamades” wine provided a richer profile than “Karnachalades”. To our knowledge, this is the first study that couples both molecular profiling and exploration of the enological potential of the rare Greek varieties “Karnachalades” and “Bogialamades”; they represent two promising varieties for the production of red wines in the historic region of Thrace.

scholarly journals A note on genetic parameters and accuracy of estimated breeding values in honey bees

2019 ◽  
Vol 51 (1) ◽  
Author(s):  
Evert W. Brascamp ◽  
Piter Bijma
Keyword(s):  
Honey Bees ◽  
Variance Components ◽  
Genetic Parameters ◽  
Additive Genetic Variance ◽  
Breeding Value ◽  
Phenotypic Variance ◽  
Breeding Values ◽  
Estimated Breeding Values ◽  
Additive Genetic Relationship ◽  
The Relationship

Abstract Background In honey bees, observations are usually made on colonies. The phenotype of a colony is affected by the average breeding value for the worker effect of the thousands of workers in the colony (the worker group) and by the breeding value for the queen effect of the queen of the colony. Because the worker group consists of multiple individuals, interpretation of the variance components and heritabilities of phenotypes observed on the colony and of the accuracy of selection is not straightforward. The additive genetic variance among worker groups depends on the additive genetic relationship between the drone-producing queens (DPQ) that produce the drones that mate with the queen. Results Here, we clarify how the relatedness between DPQ affects phenotypic variance, heritability and accuracy of the estimated breeding values of replacement queens. Second, we use simulation to investigate the effect of assumptions about the relatedness between DPQ in the base population on estimates of genetic parameters. Relatedness between DPQ in the base generation may differ considerably between populations because of their history. Conclusions Our results show that estimates of (co)variance components and derived genetic parameters were seriously biased (25% too high or too low) when assumptions on the relationship between DPQ in the statistical analysis did not agree with reality.

scholarly journals Genetic diversity of table grape based on morphoagronomic traits

2011 ◽  
Vol 68 (1) ◽  
pp. 42-49 ◽  
Author(s):  
Patrícia Coelho de Souza Leão ◽  
Cosme Damião Cruz ◽  
Sérgio Yoshimitsu Motoike
Keyword(s):  
Genetic Diversity ◽  
Clustering Analysis ◽  
Germplasm Collection ◽  
Table Grape ◽  
Cluster Method ◽  
Breeding Programs ◽  
Method Analysis ◽  
Vitis Spp ◽  
Grape Cultivars

The conservation and characterization of grape (Vitis spp) genetic resources in germplasm banks have been the basis of its use in breeding programs that result in development of new cultivars. There are at least 10,000 grape cultivars kept in germplasm collection. The genetic diversity in 136 table grape accessions from the state of Bahia, Brazil, was evaluated. Continuous and discrete morphoagronomic traits were assessed. The clustering analysis by the Tocher otimization method resulted in 30 clusters (considering continuous morphoagronomic traits), and 9 clusters (taking into consideration multicategorical traits). There was no agreement between clusters obtained by both, continuous or discrete phenotypic descriptors, independent of the cluster method analysis used. A satisfactory genetic variability among the table grape accessions was observed.

scholarly journals Six new cases confirm the clinical molecular profile of complete combined 17α-hydroxylase/ 17,20-lyase deficiency in Brazil

2010 ◽  
Vol 54 (8) ◽  
pp. 711-716 ◽  
Author(s):  
Daiane Rodrigues Barbosa Belgini ◽  
Maricilda Palandi de Mello ◽  
Maria Tereza Matias Baptista ◽  
Daniel Minutti de Oliveira ◽  
Fernanda Canova Denardi ◽  
...  
Keyword(s):  
Present Report ◽  
Molecular Data ◽  
The Other ◽  
Renin Activity ◽  
Inguinal Region ◽  
Molecular Profile ◽  
Hydroxylase Deficiency ◽  
Lyase Deficiency ◽  
Frequent Mutations ◽  
Low Levels

In 2004, Costa-Santos and cols. reported 24 patients from 19 Brazilian families with 17α-hydroxylase deficiency and showed that p.W406R and p.R362C corresponded to 50% and 32% of CYP17A1 mutant alleles, respectively. The present report describes clinical and molecular data of six patients from three inbred Brazilian families with 17α-hydroxlyse deficiency. All patients had hypogonadism, amenorrhea and hypertension at diagnosis. Two sisters were found to be 46,XY with both gonads palpable in the inguinal region. All patients presented hypergonadotrophic hypogonadism, with high levels of ACTH (> 104 ng/mL), suppressed plasmatic renin activity, low levels of potassium (< 2.8 mEq/L) and elevated progesterone levels (> 4.4 ng/mL). Three of them, including two sisters, were homozygous for p.W406R mutation and the other three (two sisters and one cousin) were homozygous for p.R362C. The finding of p.W406R and p.R362C in the CYP17A1 gene here reported in additional families, confirms them as the most frequent mutations causing complete combined 17α-hydroxylase/17,20-lyase deficiency in Brazilian patients.

scholarly journals Brassinosteroids and gibberellic acid: effects on in vitro pollen germination in grapevine

OENO One ◽  
2017 ◽  
Vol 51 (3) ◽  
pp. 303
Author(s):  
Zeliha Gokbayrak ◽  
Hakan Engin
Keyword(s):  
Gibberellic Acid ◽  
Growth And Development ◽  
Pollen Germination ◽  
Table Grape ◽  
Basic Medium ◽  
Control Group ◽  
Plant Growth And Development ◽  
Physiological Processes ◽  
Grape Cultivars

<p style="text-align: justify;">Many physiological processes related to plant growth and development are under the influence of growth regulators, which also have an impact on pollen germination. In this study, the effects of two brassinosteroid compounds, epibrassinolide and 22S,23S-homobrassinolide, and gibberellic acid (GA<sub>3</sub>) on <em>in vitro</em> pollen germination of two table grape cultivars, ‘Italia’ and ‘Cardinal’ (<em>Vitis vinifera</em> L.), were determined. A total of 28 treatments, alone and in combination, were applied to freshly collected pollens which were sown on a basic medium with 1% agar and 20% sucrose. Petri dishes were kept at 26±1°C for 24 hours. Counting of the germinated pollens revealed that the effects of these plant hormones were cultivar- and substance-specific. The cultivar ‘Italia’ was not influenced by the treatments (the highest germination ratio being 44.4% from 0.001 mg L<sup>-1</sup> epibrassinolide) as opposed to the cultivar ‘Cardinal’. The highest germination ratio in ‘Cardinal’ was about 50% in pollens treated with 25 mg L<sup>-1</sup> GA<sub>3</sub> + 0.01 mg L<sup>-1</sup> epibrassinolide. The control group resulted in 32.38% germination. Combining GA<sub>3</sub> with epibrassinolide provided slightly higher germination ratios compared to combining GA<sub>3</sub> with 22S,23S-homobrassinolide. </p>

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