Type 2 Diabetes and its Effects on MicroRNA-155 and MicroRNA-133a Expression in Lung Tissue of Male Rats
Abstract Background: The presence of an extensive vascular extent and large amounts of collagen and elastin in lung tissue make the lung parenchyma vulnerable to damage in diabetes. However, there are few studies on the pathophysiological effects of diabetes on lung tissue. In this study, we investigated the effects of type 2 diabetes on lung tissue pathology and the expression of miRNA-155 and miRNA-133a in lung tissue of male rats.Methods: In this study, 14 male rats were divided into a control group and a group with diabetes. The group with diabetes received a high-fat diet and a single dose of streptozotocin for one month. After induction of T2D, the rats received a normal diet for the next four weeks. At week 8, the rats were euthanized, and lung tissue was collected to measure microRNAs and examine tissue changes.Results: When lung tissue sections from diabetic rats were examined, the normal structure of the alveoli and alveolar sacs and bronchioles was disturbed. The thickness of interalveolar septa was increased due to the infiltration of inflammatory cells. The extensive alveolar collapse was the main cause of lung tissue structure disruption, and the accumulation of inflammatory cells and exudate secretions resulted in an interstitial pneumonia-like appearance. The expression of miRNA-155 was increased, and the expression of miRNA-133a was decreased in the lungs of diabetic rats compared with control rats.Conclusion: We found significant changes in the lung tissue of diabetic rats. By studying changes in the expression of microRNAs in diabetes, they can be a diagnostic and therapeutic biomarker in the lungs of patients with diabetes.