scholarly journals 947 SCREENING MARIGOLD CULTIVARS FOR SUSCEPTIBILITY TO IRON TOXICITY

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 569d-569 ◽  
Author(s):  
Nancy K. Todd

Marigolds are susceptible to a specific nutritional disorder known as “bronze speckling”. It has been reported that the disorder is caused by excessive uptake of iron by the plant, which may be due to high levels of Fe in the soil solution or low soil pH. In this experiment, 12 cultivars of marigold (Tagetes erecta and T. patula) were grown using increasing levels of Fe (0, 5, 15, and 20 mg/l) from Fe DTPA. In the susceptible cultivars, symptoms were observed within 5 days of initial treatment and appeared as a chlorotic mottling. Initial symptoms resembled spider mite damage on older leaves, which gradually became bronze colored in appearance, and finally became necrotic. Downward cupping of leaves was observed in severely affected plants. Severity of necrosis and percent of plant leaves affected (dry weight basis) were evaluated to determine susceptibility of the different cultivars to the disorder. There was a direct correlation between increasing concentration of Fe and occurrence and severity of symptoms. The most susceptible to least susceptible cultivars were determined to be: First Lady, Inca, Discovery, Galore, Pineapple Crush, Perfection Excel, Voyager All Seasons, Nugget, Zenith, Voyager F1 and Diamond Jubilee.

1980 ◽  
Vol 60 (2) ◽  
pp. 275-284 ◽  
Author(s):  
J. L. DIONNE

Aflalfa (Medicago sativa L. ’Saranac’) was grown under greenhouse conditions in order to determine changes in magnesium (Mg) response and availability following variation in soil pH. The rates of Mg applications were 0, 25, 50, 100 and 200 ppm of Mg calculated on soil dry weight basis. Soil pH was adjusted to 5.0 (or original) 6.0, 6.5 and 7.5. A 40% yield increase was recorded on Greensboro loam following Mg fertilization. The increase in yields due to Mg was 23% on the two others soil types. The Mg contents of plant and soil increased with rate of Mg applied to soil. In general, the response of alfalfa was increased by liming soils and the Mg content of alfalfa was decreased. The availability of Mg in soils was also lowered by liming. The Mg requirement of soils varied from 25 to 100 ppm depending upon pH and soil types.


Author(s):  
I.M. Ritchie ◽  
C.C. Boswell ◽  
A.M. Badland

HERBACE DISSECTION is the process in which samples of herbage cut from trials are separated by hand into component species. Heavy reliance is placed on herbage dissection as an analytical tool ,in New Zealand, and in the four botanical analysis laboratories in the Research Division of the Ministry of Agriculture and Fisheries about 20 000 samples are analysed each year. In the laboratory a representative subsample is taken by a rigorous quartering procedure until approximately 400 pieces of herbage remain. Each leaf fragment is then identified to species level or groups of these as appropriate. The fractions are then dried and the composition calculated on a percentage dry weight basis. The accuracy of the analyses of these laboratories has been monitored by a system of interchanging herbage dissection samples between them. From this, the need to separate subsampling errors from problems of plant identification was, appreciated and some of this work is described here.


1989 ◽  
Vol 21 (2) ◽  
pp. 161-165 ◽  
Author(s):  
S. I. Kayal ◽  
D. W. Connell

Results of the analysis of twenty-three composite sediment samples revealed that PAHs are widely distributed in the Brisbane River estuary. Mean concentrations for individual compounds, on a dry weight basis, ranged from 0.03 µg/g for dibenz [ah] anthracene to 2.34 µg/g for fluoranthene. Observed PAH assemblages were rich in compounds having pyrolytic origins. However, the presence of petroleum derived compounds was indicative of the importance of petroleum as a PAH source in the estuary. Petroleum refineries, a coal loading terminal and a major treated sewage outfall located at the mouth were not indicated as major contributing sources of PAH pollution in the estuary.


1985 ◽  
Vol 15 (5) ◽  
pp. 855-861 ◽  
Author(s):  
G. Prégent ◽  
C. Camiré

Invitro cultures of Alnuscrispa (Ait.) Pursh and Alnusglutinosa (L.) Gaertn. were used to estimate critical foliage levels of selected nutrients for optimal growth and dinitrogen (N2) fixation. For A. crispa to obtain 90% of maximum growth and N2 fixation, foliar levels of 0.12% P, 0.13% Mg, <0.31% K, and <0.04% Ca on a dry weight basis were needed. For A. glutinosa, the critical levels were 0.138% P, 0.10% Mg, 0.29% Ca, and ~0.20% K. From all the deficiencies observed, P had the more pronounced effects on N status of both species.


1983 ◽  
Vol 63 (2) ◽  
pp. 415-420 ◽  
Author(s):  
D. G. GREEN

Alfa, a relatively nonhardy alfalfa cultivar continued to accumulate, on a dry weight basis, fructose, α- and β-D-glucose, sucrose and maltose during the latter stages of cold hardening. Rambler, a hardier alfalfa cultivar conversely showed a decrease for these soluble sugars with hardening. Frontier rye, a very hardy winter habit cereal showed decreases in these soluble sugars plus melibiose during the same hardening period. These results support the hypothesis that hardy cereals and alfalfa undergo a decrease in soluble sugars with hardening, while less hardy cereals and alfalfa continue to increase in content of soluble sugars. Manitou wheat appeared not to fit this hypothesis and showed the decreased soluble sugars usually associated with hardy cultivars. Although Manitou is a spring type wheat, one of its parents, Thatcher, does contain gene(s) for the winter habit.Key words: Sugar, cold hardening, wheat, rye, alfalfa


1954 ◽  
Vol 37 (3) ◽  
pp. 381-399 ◽  
Author(s):  
Ruth Hubbard

The sedimentation behavior of aqueous solutions of digitonin and of cattle rhodopsin in digitonin has been examined in the ultracentrifuge. In confirmation of earlier work, digitonin was found to sediment as a micelle (D-1) with an s20 of about 6.35 Svedberg units, and containing at least 60 molecules. The rhodopsin solutions sediment as a stoichiometric complex of rhodopsin with digitonin (RD-1) with an s20 of about 9.77 Svedberg units. The s20 of the RD-1 micelle is constant between pH 6.3 and 9.6, and in the presence of excess digitonin. RD-1 travels as a single boundary also in the electrophoresis apparatus at pH 8.5, and on filter paper at pH 8.0. The molecular weight of the RD-1 micelle lies between 260,000 and 290,000. Of this, only about 40,000 gm. are due to rhodopsin; the rest is digitonin (180 to 200 moles). Comparison of the relative concentrations of RD-1 and retinene in solutions of rhodopsin-digitonin shows that RD-1 contains only one retinene equivalent. It can therefore contain only one molecule of rhodopsin with a molecular weight of about 40,000. Cattle rhodopsin therefore contains only one chromophore consisting of a single molecule of retinene. It is likely that frog rhodopsin has a similar molecular weight and also contains only one chromophore per molecule. The molar extinction coefficient of rhodopsin is therefore identical with the extinction coefficient per mole of retinene (40,600 cm.2 per mole) and the E(1 per cent, 1 cm., 500 mµ) has a value of about 10. Rhodopsin constitutes about 14 per cent of the dry weight, and 3.7 per cent of the wet weight of cattle outer limbs. This corresponds to about 4.2 x 106 molecules of rhodopsin per outer limb. The rhodopsin content of frog outer limbs is considerably higher: about 35 per cent of the dry weight, and 10 per cent of the wet weight, corresponding to about 2.1 x 109 molecules per outer limb. Thus the frog outer limb contains about five hundred times as much rhodopsin as the cattle outer limb. But the relative volumes of these structures are such that the ratio of concentrations is only about 2.5 to 1 on a weight basis. Rhodopsin accounts for at least one-fifth of the total protein of the cattle outer limb; for the frog, this value must be higher. The extinction (K500) along its axis is about 0.037 cm.2 for the cattle outer limb, and about 0.50 cm.2 for the frog outer limb.


1985 ◽  
Vol 48 (12) ◽  
pp. 1040-1043 ◽  
Author(s):  
P. E. KOEHLER ◽  
L. R. BEUCHAT ◽  
M. S. CHHINNAN

Experiments were done to determine the influence of temperature (21, 30 and 37°C) and aw (0.76 to 0.98) on aflatoxin production by Aspergillus flavus on cowpea (Vigna unguiculata) seeds, meal and meal supplemented with onion. Larger quantities of aflatoxin were produced at 21 and 30°C than at 37°C. The highest amount of aflatoxin (2777 μg/20 g, dry weight basis) was observed in meal containing onion at aw 0.98 after 20 d of incubation at 21°C. A level of 870 |μg/20 g was detected in seeds at aw 0.95 after 14 d of incubation at 30°C. Meal at aw 0.96 supported production of 551 μg of aflatoxin per 20 g after 20 d at 30° C. Temperature had little influence on the optimal aw for aflatoxin production in cowpea meal. However, an increase in temperature resulted in a decreased optimal aw for aflatoxin production on whole cowpeas. When known quantities of aflatoxin were added to cowpea meal which was subsequently steamed for 5 min, only 29% was extractable using a variety of procedures, indicating that the toxin may be bound in some manner to cowpea constituents as a result of heat treatment.


1977 ◽  
Vol 55 (4) ◽  
pp. 489-495 ◽  
Author(s):  
Sherman D. Nelson ◽  
James M. Mayo

Abaxial epidermal strips from leaves of Paphiopedilum leeanum were analyzed via sodium cobaltinitrite staining and atomic absorption spectrophotometry for the presence and location of potassium. On a dry weight basis K content of the abaxial epidermis was found to be 103 times less than has been reported in other species, and unlike other species no localization of K+ in guard cells of open stomata could be detected via the sodium cobaltinitrite stain for potassium.Flame photometric analysis of the mesophyll indicated that it contained normal amounts of K+ (about 1.87% on a dry weight basis). Analysis showed that the K+ content of the abaxial epidermis (0.032%) was considerably less than that of the mesophyll, a situation unlike previous reports for other species in which the epidermal concentration was found to be greater than the mesophyll. A process for exclusion of K+ from the abaxial epidermis is suggested, as is the lack of involvement of K+ as the major osmoticum in the stomatal mechanism of this species.


2016 ◽  
Author(s):  
Abdulaha-Al Baquy ◽  
Jiu-Yu Li ◽  
Chen-Yang Xu ◽  
Khalid Mehmood ◽  
Ren-Kou Xu

Abstract. Soil acidity has become a serious constraint in dry land crop production systems of acidic Ultisols in tropical and subtropical regions of southern China, where winter wheat and canola are cultivated as important rotational crops. Regardless of other common existing concerns in acidic Ultisols of southern China, it needs to be investigated whether soil acidity has any effect on wheat and canola growth. There is little information on the determination of critical soil pH as well as aluminium (Al) concentration for wheat and canola crops. The objective of this study was to determine the critical soil pH and exchangeable aluminium concentration (AlKCl) for wheat and canola production. Two pot cultures with two Ultisols from Hunan and Anhui were conducted for wheat and canola crops in a controlled growth chamber, with a completely randomized design. A soil pH gradient ranging from 3.7 (Hunan) and 3.97 (Anhui) to 6.5, with three replications, was used as a treatment. Aluminium sulfate (Al2(SO4)3) and hydrated lime (Ca(OH)2) were used to obtain the target soil pH levels. Plant height, shoot dry weight, root dry weight, and chlorophyll content (SPAD value) of wheat and canola were adversely affected by soil acidity in both locations. The critical soil pH and AlKCl of the Ultisol from Hunan for wheat were 5.29 and 0.56 cmol kg−1, respectively. At Anhui, the threshold soil pH and AlKCl for wheat were 4.66 and 2.36 cmol kg−1, respectively. On the other hand, the critical soil pH for canola was 5.65 and 4.87 for the Ultisols from Hunan and Anhui, respectively. The critical soil exchangeable Al for canola cannot be determined from the experiment of this study. The results suggested that the critical soil pH and AlKCl varied between different locations for the same variety of crop, due to the different soil types and their other soil chemical properties. The critical soil pH for canola was higher than that for wheat for both Ultisols, thus canola was more sensitive to soil acidity. Therefore, we recommend that liming should be undertaken to increase soil pH if it falls below these critical soil pH levels for wheat and canola production.


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