scholarly journals GIBBERELLIC ACID DURING PRIMING OF PURPLE CONEFLOWER [ECHINACEA PURPUREA (L) MOENCH.] SEEDS IMPROVES GERMINATION AND SEEDLING EMERGENCE

HortScience ◽  
1995 ◽  
Vol 30 (2) ◽  
pp. 186c-186
Author(s):  
Janine O. Haynes ◽  
Wallace G. Pill
Keyword(s):  
Gibberellic Acid ◽  
Germination Rate ◽  
Seedling Emergence ◽  
Dry Weight ◽  
Echinacea Purpurea ◽  
Germination Percentage ◽  
Shoot Dry Weight ◽  
Greenhouse Study ◽  
Purple Coneflower ◽  
Percentage Points

Purple coneflower seeds following priming (-0.04 MPa, 10 days, 15C, darkness) osmotically in polyethylene glycol 8000 (PEG) or matrically in expanded no. 5 vermiculite had greater germination rate and synchrony at continuous 20C or 30C than untreated seeds, but germination percentage was unaffected. Inclusion of 5.5 × 10-2 M gibberellic acid (GA3 as ProGibb Plus 2X, Abbott Laboratories, N. Chicago, Ill.) further improved germination rate and synchrony at 20C, but not at 30C. In a greenhouse study (30C day/27C night, July-August natural light), seeds primed in PEG or vermiculite containing G A3 compared to untreated seeds had 6 percentage points higher maximum emergence (ME), 3.3 fewer days to 50% ME, 1.9 fewer days between 10% and 90% ME, 116% greater shoot dry weight, and 125% longer leaves at 16 days after planting in peat-lite. Inclusion of ethephon (0.01 m, as Florel) either alone or with GA3 during priming provided no benefit to seed germination or seedling emergence. Moistened vermiculite substituted for PEG solution as a priming medium for purple coneflower seeds, the priming benefit on seedling emergence and growth being enhanced by 5.5 × 10-2m G A3 inclusion in the priming media.

scholarly journals Germination and Emergence of Parsley in Response to Osmotic or Matric Seed Priming and Treatment with Gibberellin

HortScience ◽  
2000 ◽  
Vol 35 (5) ◽  
pp. 907-909 ◽  
Author(s):  
Wallace G. Pill ◽  
Elizabeth A. Kilian
Keyword(s):  
Polyethylene Glycol ◽  
Gibberellic Acid ◽  
Seedling Emergence ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Petroselinum Crispum ◽  
Hypocotyl Length ◽  
Shoot Dry Weight ◽  
Emergence Percentage

`Moss Curled' seeds of parsley (Petroselinum crispum L.) were primed osmotically in polyethylene glycol or matrically in fine, exfoliated vermiculite at –0.5 MPa for 4 or 7 days at 20 or 30 °C with 0 or 1 mm GA3. All priming treatments stimulated and hastened germination. Matric priming resulted in greater germination (89%) than osmotic priming (83%) when seeds were primed for 7 days at 30 °C, but priming agent had no effect on germination percentage following priming at 20 °C or for 4 days. In seeds primed for 4 days at 20 or 30 °C, matric priming hastened germination more than did osmotic priming. Germination was generally less synchronous with matric than with osmotic priming. Increasing priming time from 4 to 7 days increased the rate of germination, but increased germination synchrony only when seeds were primed at 20 °C. Inclusion of 1 mm GA3 during priming had little or no effect on germination. All matric priming treatments (other than 4-day priming) were repeated to assess seedling emergence in a greenhouse (25°C day/22 °C night). Priming increased the percentage, rate and synchrony of emergence, and increased hypocotyl length at 3 weeks after planting. Priming at 30 °C with 1 mm GA3 resulted in the greatest emergence percentage, hypocotyl length, and shoot dry weight. We conclude that matric priming is a satisfactory alternative to osmotic priming of parsley seeds. Chemical name used: gibberellic acid (GA3).

scholarly journals Germination and Emergence of Parsley in Response to Osmotic or Matric Seed Priming and Treatment with Gibberellin

2001 ◽  
Vol 11 (1) ◽  
pp. 152
Author(s):  
Wallace G. Pill ◽  
Elizabeth A. Kilian
Keyword(s):  
Polyethylene Glycol ◽  
Gibberellic Acid ◽  
Seedling Emergence ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Petroselinum Crispum ◽  
Hypocotyl Length ◽  
Shoot Dry Weight ◽  
Emergence Percentage

`Moss Curled' seeds of parsley (Petroselinum crispum L.) were primed osmotically in polyethylene glycol or matrically in fine, exfoliated vermiculite at -0.5 MPa for 4 or 7 days at 20 or 30 °C with 0 or 1 mm GA3. All priming treatments stimulated and hastened germination. Matric priming resulted in greater germination (89%) than osmotic priming (83%) when seeds were primed for 7 days at 30 °C, but priming agent had no effect on germination percentage following priming at 20 °C or for 4 days. In seeds primed for 4 days at 20 or 30 °C, matric priming hastened germination more than did osmotic priming. Germination was generally less synchronous with matric than with osmotic priming. Increasing priming time from 4 to 7 days increased the rate of germination, but increased germination synchrony only when seeds were primed a t 20 °C. Inclusion of 1 mm GA3 during priming had little or no effect on germination. All matric priming treatments (other than 4-day priming) were repeated to assess seedling emergence in a greenhouse (25°C day/22 °C night). Priming increased the percentage, rate and synchrony of emergence, and increased hypocotyl length at 3 weeks after planting. Priming at 30 °C with 1 mm GA3 resulted in the greatest emergence percentage, hypocotyl length, and shoot dry weight. We conclude that matric priming is a satisfactory alternative to osmotic priming of parsley seeds. Chemical name used: gibberellic acid (GA3).

scholarly journals Seed Source and Quality Influence Germination in Purple Coneflower [Echinacea purpurea (L.) Moench.]

HortScience ◽  
1994 ◽  
Vol 29 (12) ◽  
pp. 1443-1444 ◽  
Author(s):  
N. Wartidiningsih ◽  
R.L. Geneve
Keyword(s):  
Seed Germination ◽  
Dry Weight ◽  
Echinacea Purpurea ◽  
Germination Percentage ◽  
Physiological Maturity ◽  
Seed Sources ◽  
Purple Coneflower ◽  
Temperature Regimes ◽  
Two Temperature ◽  
Effect Of Light

Six seed lots of purple coneflower were purchased from four commercial seed sources and evaluated for germination in either light or darkness in combination with two temperature regimes (constant 27C vs. alternating 30C for 8 hours and 20C for 16 hours). Seed lot differences accounted for the majority of variation, with two seed lots exhibiting high (81% to 91%) germination and the remaining seed lots having 39% to 66% germination. There was no effect of light on germination, regardless of the seed lot. However, alternating temperatures improved germination in one of the low-germination seed lots. Seed size and inflorescence position did not affect seed germination. Seed harvested at physiological maturity (maximum seed dry weight), but before drying had occurred, had a higher germination percentage than seeds harvested after desiccation, and they maintained a higher germination percentage even after 1 year in storage.

scholarly journals Matric Priming of Kentucky Bluegrass and Tall Fescue Seeds Benefits Seedling Emergence

HortScience ◽  
1997 ◽  
Vol 32 (6) ◽  
pp. 1061-1063 ◽  
Author(s):  
Wallace G. Pill ◽  
John J. Frett ◽  
Ian H. Williams
Keyword(s):  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Poa Pratensis ◽  
Germination Rate ◽  
Seedling Emergence ◽  
Dry Weight ◽  
Kentucky Bluegrass ◽  
Germination Percentage ◽  
Weight Basis

Matrically priming seeds of common Kentucky bluegrass (Poa pratensis L.) and `SR8300' tall fescue (Festuca arundinacea Schreb.) in fine, exfoliated vermiculite (-1.5 MPa, 20 °C, 4 days) increased subsequent germination rate but did not increase germination percentage or synchrony. The lowest seed: vermiculite ratio (dry weight basis) to provide full priming benefit for seeds of Kentucky bluegrass and tall fescue was 1:10 and 1:20, respectively. Storing Kentucky bluegrass seeds primed in 1:10 (seed:vermiculite) in moist vermiculite for 10 days at either 5 or 20 °C did not reduce germination rate in comparison to primed seeds that were not stored. Primed tall fescue seeds could be stored in moist vermiculite (1:20, seed:vermiculite) for up to 10 days at 5 °C with no loss of priming benefit, but storage for only 2 days at 20 °C resulted in germination. Primed seeds of Kentucky bluegrass (stored for 0 or 10 days at 5 or 20 °C) or tall fescue (stored 0 or 10 days at 5 °C or 2 days at 20 °C) resulted in more rapid germination and seedling emergence, and greater seedling shoot fresh and dry masses than was the case for nonprimed seeds.

scholarly journals MATRIC AND OSMOTIC PRIMING OF PURPLE CONEFLOWER

HortScience ◽  
1993 ◽  
Vol 28 (4) ◽  
pp. 256F-256
Author(s):  
Cynthia Crossan ◽  
Wallace Pill
Keyword(s):  
Polyethylene Glycol ◽  
Growth Regulators ◽  
Seedling Emergence ◽  
Echinacea Purpurea ◽  
Germination Percentage ◽  
Solid Matrix ◽  
Greenhouse Trial ◽  
Purple Coneflower ◽  
Expanded Vermiculite ◽  
Emergence Rate

Seeds of purple coneflower (Echinacea purpurea (L.) Moench were osmotically primed (OSP) in polyethylene glycol (PEG) or matrically primed in expanded vermiculite No. 5 (solid matrix priming, SMP). With both OSP or SHP at 15C, 10-day exposure to -0.4 MPa resulted in lowered time to 50% germination (T,) and higher germination percentage than shorter exposure (5-day) or lower water potential (-1.5 MPa). SMP- and OSP-seeds performed similarly in a greenhouse trial, resulting in 80% and 34% seedling emergence at 23C and 37C, respectively, compared to 58% and 27% for non-primed seeds. Seedling emergence rate and synchrony from primed seeds were greater than from non-primed seeds at both temperatures. An incubator study established that adding 10-4M GA3 and 10 mN ethephon (2-chloroethylphosphonic acid) to the PEG or vermiculite resulted in lower T50 and higher germination percentage than priming without these growth regulators. A further incubator study established that less-expensive trade products (Pro-Gibb Pius 2X) and Florel could substitute for the reagent-grade growth regulators. Seeds primed in PEG or vermiculite containing 10-4M GA3 from Pro-Gibb Plus 2X and 10 mM ethephon from Floral had lower T50 and higher percentage emergence in a greenhouse trial than seeds primed without growth regulators. Compared to the non-primed seeds, these treated seeds had 29% greater seedling emergence and 61% less time to 50% emergence.

Salinity Tolerance of Black Gram Cultivars During Germination and Early Seedling Growth

2018 ◽  
Vol 51 (3) ◽  
pp. 51-68 ◽  
Author(s):  
M.K. Hasan ◽  
M.S. Islam ◽  
M.R. Islam ◽  
H.N. Ismaan ◽  
A. El Sabagh
Keyword(s):  
Salt Stress ◽  
Salinity Tolerance ◽  
Seedling Growth ◽  
Negative Impact ◽  
Germination Rate ◽  
Dry Weight ◽  
Germination Percentage ◽  
Shoot Length ◽  
Sand Culture ◽  
Black Gram

Abstract A laboratory experiment regarding germination and seedling growth test was conducted with three black gram genotypes tested under three salinity levels (0, 75 and 150 mM), for 10 days, in sand culture within small plastic pot, to investigate the germination and seedling growth characteristics. Different germination traits of all black gram genotypes, like germination percentage (GP), germination rate (GR), coefficient of velocity of germination (CVG) greatly reduced, as well as mean germination time (MGT) increased with increasing salt stress. At high salt stress, BARI Mash-3 provided the highest GP reduction (28.58%), while the lowest was recorded (15.79% to control) in BARI Mash-1. Salinity have the negative impact on shoot and root lengths, fresh and dry weights. The highest (50.32% to control) and lowest reduction (36.39%) of shoot length were recorded in BARI Mash-2 and BARI Mash-1, respectively, under 150 mM NaCl saline conditions. There were significant reduction of root lengths, root fresh and dry weight, shoot length, shoot fresh and dry weight in all genotypes under saline condition. The genotypes were arranged as BARI Mash-1 > BARI Mash-3 > BARI Mash-2, with respect to salinity tolerance.

scholarly journals Phytotoxicity of Chitosan and SiO2 Nanoparticles to Seed Germination of Wheat (Triticum aestivum L.) and Barley (Hordeum vulgare L.) Plants

2017 ◽  
Vol 9 (2) ◽  
pp. 242-249 ◽  
Author(s):  
Faride BEHBOUDI ◽  
Zeinalabedin TAHMASEBI SARVESTANI ◽  
Mohamad Zaman KASSAEE ◽  
Seyed Ali Mohamad MODARES SANAVI ◽  
Ali SOROOSHZADEH
Keyword(s):  
Germination Rate ◽  
Dry Weight ◽  
Sio2 Nanoparticles ◽  
Triticum Aestivum L ◽  
Germination Percentage ◽  
Hordeum Vulgare L ◽  
Randomized Design ◽  
Direct Exposure ◽  
Contrast Exposure ◽  
Mean Germination Time

Plants such as wheat and barley that are strategically important crops need to be considered to develop a comprehensive toxicity profile for nanoparticles (NPs). The present study was aimed to investigate the effects of chitosan and SiO2 NPs on wheat and barley plants. Two factorial experiments (seeds priming and direct exposure) were performed based on a completely randomized design in four replications. Results showed that the seeds priming with the NPs had not significant effect on germination parameters such as Germination Percentage (GP), Germination Rate (GR), Germination Value (GV), Mean Germination Time (MGT), Pick Value (PV) and Mean Daily Germination (MDG). In contrast, exposure of the seeds to the NPs had significant effects on these parameters. In both experiments, treatments had significant effects on shoot, seedling, root length, fresh and dry weight, as well as vigor indexes as compared to the control. In most traits, the best concentration of NPs was 30 ppm, whereas applications of the NPs with 90 ppm displayed adverse effects on majority of the studied traits. According to these results, selectivity in applications of NPs with suitable concentration and method is essential for different plant species.  

scholarly journals Exogenous Antioxidants Enhance Seedling Growth and Yield of Artificially Aged Cabbage and Lettuce Seeds

Horticulturae ◽  
2021 ◽  
Vol 7 (9) ◽  
pp. 274
Author(s):  
Ademola Adetunji ◽  
Sershen ◽  
Boby Varghese ◽  
Norman Pammenter
Keyword(s):  
Leaf Area ◽  
Seedling Growth ◽  
Seedling Emergence ◽  
Dry Weight ◽  
Growth And Yield ◽  
Seed Vigour ◽  
Beneficial Effects ◽  
Shoot Dry Weight ◽  
Antioxidant Defence System ◽  
Aged Seeds

Aged seeds exhibit compromised vigour in terms of germination, seedling emergence and growth, but this can to some extent be alleviated by invigoration treatments before sowing. This study aimed to investigate ageing rates and patterns in cabbage (Brassica oleraceae) and lettuce (Lactuca sativa) seeds and whether the beneficial effects of invigorating aged seeds with exogenous antioxidants translate to enhanced seedling performance. Seeds were artificially aged to 25% viability before soaking in 0.4 mM glycerol, 0.6 mM GSH and 0.2 mM trolox for cabbage, and 0.6 mM glycerol, GSH and trolox for lettuce; deionised water served as a control. After 14 days of sowing, seedling emergence percentage, mean emergence time, mean daily emergence, and time taken to 25% emergence were computed. Seedling vigour index, root and shoot dry weight, root:shoot ratio, leaf area, leaf area ratio, and leaf chlorophyll content were assessed 6 weeks after sowing. Furthermore, the photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (E), and chlorophyll fluorescence were measured 6 weeks after sowing. Notably, ageing resulted in the loss of seed vigour and viability at higher rates in lettuce than cabbage. Seed pretreatment with glycerol promoted seedling growth in both species and shoot dry weight in lettuce, while glycerol and GSH enhanced Pn, Gs and E in lettuce. Trolox also enhanced Pn and E in lettuce. The beneficial effects of the antioxidant treatments are thought to be associated with the protection of photosystems from oxidative stress and/or stimulation of enzymes involved in photosynthesis, possibly through an enhanced antioxidant defence system during the early development stages when seedlings are particularly vulnerable to stress.

Confirmation of Glyphosate-Resistant Horseweed (Conyza canadensis) in Montana Cereal Production and Response to POST Herbicides

2017 ◽  
Vol 31 (6) ◽  
pp. 799-810 ◽  
Author(s):  
Vipan Kumar ◽  
Prashant Jha ◽  
Amit J. Jhala
Keyword(s):  
Great Plains ◽  
Dose Response ◽  
Best Management Practices ◽  
Management Practices ◽  
Dry Weight ◽  
Shoot Dry Weight ◽  
Greenhouse Study ◽  
Whole Plant ◽  
Cereal Production ◽  
Sites Of Action

In recent years, horseweed has become an increasing problem in Montana. To confirm and characterize the level of glyphosate resistance, seeds were collected from putative glyphosate-resistant (GR) horseweed (GR-MT) plants in a wheat–fallow field in McCone County, MT. Known GR (GR-NE) and glyphosate-susceptible (GS-NE) horseweed accessions from Lincoln, NE, were included for comparison in dose–response and shikimate accumulation studies. Whole-plant glyphosate dose–response experiments conducted at the early- (5- to 8-cm diameter) and late- (12- to 15-cm diameter) rosette stages of horseweed indicated that GR-MT accessions had a 2.5- to 4.0-fold level of resistance to glyphosate relative to the GS-NE accession, on the basis of shoot dry weight (GR50values). The level of resistance was 3.1- to 7.9-fold on the basis of visually assessed injury estimates (I50values). At the whole-plant level, about 2.1- to 4.5-fold higher shikimate accumulation was observed in the GS-NE accession compared with the GR-MT and GR-NE accessions over a 10-d period after glyphosate was applied at 1,260 g ae ha−1. In a separate greenhouse study, all three horseweed accessions were also screened with alternate POST herbicides registered for use in wheat–fallow rotations. The majority of the tested herbicides provided ≥90% injury at the field-use rates for all three horseweed accessions 3 wk after treatment. This is the first published report on the occurrence of GR horseweed in Montana cereal production. Increased awareness and adoption of best management practices, including the use of diversified (based on multiple sites of action) herbicide programs highlighted in this study, would aid in mitigating the further spread of GR horseweed in the cereal production fields of the U.S. Great Plains.

scholarly journals Effect of Seed Priming on Germination Properties and Seedling Establishment of Cowpea (Vigna sinensis)

2011 ◽  
Vol 3 (4) ◽  
pp. 113-116 ◽  
Author(s):  
Hamdollah ESKANDARI ◽  
Kamyar KAZEMI
Keyword(s):  
Laboratory Tests ◽  
Low Cost ◽  
Germination Rate ◽  
Seedling Emergence ◽  
Laboratory Data ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Seedling Vigor ◽  
Contributing Factors

Early emergence and stand establishment of cowpea are considered to be the most important yield-contributing factors in rainfed areas. Laboratory tests and afield experiment were conducted in RCB design in 2011 at a research farm in Ramhormoz, Iran, to evaluate the effects of hydropriming (8, 12 and 16 hours duration) and halo priming (solutions of 1.5% KNO3 and 0.8% NaCl) on seedling vigor and field establishment of cowpea. Analysis of variance of laboratory data showed that hydropriming significantly improved germination rate, seed vigor index, and seedling dry weights. However, germination percentage for seeds primed with KNO3 and non-primed seeds were statistically similar, but higher than those for NaCl priming. Overall, hydropriming treatment was comparatively superior in the laboratory tests. Invigoration of cowpea seeds by hydropriming and NaCl priming resulted in higher seedling emergence and establishment in the field, compared to control and seed priming with KNO3. Seedling emergence rate was also enhanced by priming seeds with water, suggesting that hydropriming is a simple, low cost and environmentally friendly technique for improving seed and seedling vigor of cowpea.

Sign in / Sign up

Export Citation Format

Share Document