scholarly journals Gibberellic Acid and Presowing Chilling Increase Seed Germination of Indiangrass [Sorghastrum nutans (L.) Nash.]

HortScience ◽  
1998 ◽  
Vol 33 (5) ◽  
pp. 849-851 ◽  
Author(s):  
Jonathon I. Watkinson ◽  
Wallace G. Pill
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Sodium Hypochlorite ◽  
Seedling Emergence ◽  
Dry Mass ◽  
Sorghastrum Nutans ◽  
Dormancy Breaking ◽  
Germination Test ◽  
Dry Storage

Following dry storage for 5 or 11 months (new and old seeds, respectively) at 5 °C, less than 10% of the seeds of Indiangrass germinated as determined by a standard germination test. We attempted to increase germination by subjecting seeds to dormancy-breaking treatments, including sodium hypochlorite soak (5.25% v/v NaOCl; 20 or 60 min), prechilling (5 °C for 2 weeks), gibberellic acid during germination (GA3, 1000 mg·L-1), and combinations thereof. Treatment with NaOCl increased the germination of non-prechilled seeds only when they were germinated in GA3; a 60-min soak in NaOCl increased germination to 53% and 65% in new and old seeds, respectively. Prechilling increased germination to 65% and 47% in new and old seeds, respectively. Germination of new, prechilled seeds was increased further to 86% by either a 20-min soak in NaOCl or germination in GA3. Germination of old, prechilled seeds was not promoted further by treatment with NaOCl, but was increased to 67% by germination in GA3. Since NaOCl treatment alone failed to promote germination, we examined the effects on seedling emergence and growth of providing GA3 at 1000 mg·L-1 during the 2-week prechilling period. While prechilling alone increased emergence to an average 34% for new and old seeds, prechilling with GA3 increased emergence to 75% and 50% for new and old seeds, respectively. These treatments did not influence seedling shoot dry mass. Seed exposure to GA3 during rather than after prechilling was more effective in promoting Indiangrass establishment.

scholarly journals Seed germination and antioxidant enzyme activity in seedlings of diploid and tetraploid bahiagrass under water restriction

2020 ◽  
Vol 50 (5) ◽  
Author(s):  
Gabriel Streck Bortolin ◽  
Alfredo José Salinas-Arcienega ◽  
Yutcelia Carolina Galviz-Fajardo ◽  
Luciano do Amarante ◽  
Carlos Eduardo da Silva Pedroso ◽  
...  
Keyword(s):  
Seed Germination ◽  
Physiological Responses ◽  
Dry Mass ◽  
Germination Percentage ◽  
Early Growth ◽  
Paspalum Notatum ◽  
Water Restriction ◽  
Germination Test ◽  
Growth Activity ◽  
Higher Temperature

ABSTRACT: The objective of the following research was to investigate the physiological responses of two bahiagrass genotypes (Paspalum notatum Flügge) submitted to water restriction, induced by polyethylene glycol (PEG6000), during seed germination. Seeds of cv. Pensacola (diploid) and Bagual ecotype (tetraploid) were placed on paper moistened with osmotic solutions at potentials of 0.0; -0.1; -0.2 and -0.3 MPa. Composed of two subsamples of 100 seeds and four experimental units per treatment, the germination test was conducted in a germination chamber under alternating temperatures of 30 °C (8 hours) and 20 °C (16 hours), with presence of light during the higher temperature. Final germination percentage and germination speed index of the seeds, as well as early growth, activity and expression of antioxidant enzymes of seedlings were evaluated. The water restriction induced by PEG6000 reduced the percentage of germination in Pensacola (from 74% to 59%) and Bagual (34% to 10%). For the latter, the evaluation of seedlings showed higher dry mass in relation to cv. Pensacola (1.7 times higher in root and 1.6 times in shoot). In response to moderate water restriction, the genotypes showed increases in root growth (1.7 times higher in -0.1 compared to 0.0 MPa), both in expression and enzymatic activity (superoxide dismutase and catalase).

scholarly journals Factors Affecting Seed Dormancy and Germination of Greater Bur-Parsley (Turgenia latifolia)

2018 ◽  
Vol 36 ◽  
Author(s):  
M. REZVANI ◽  
S.A. SADATIAN ◽  
H. NIKKHAHKOUCHAKSARAEI
Keyword(s):  
Seed Germination ◽  
Environmental Factors ◽  
Seed Dormancy ◽  
Seedling Emergence ◽  
Potassium Nitrate ◽  
Germination Percentage ◽  
Dormancy Breaking ◽  
Planting Depth ◽  
Factors Affecting ◽  
Drought And Salt Stresses

ABSTRACT: Our knowledge about seed dormancy breaking and environmental factors affecting seed germination of greater bur-parsley (Turgenia latifolia) is restricted. This study has addressed some seed dormancy breaking techniques, including different concentrations of gibberellic acid (GA3) and potassium nitrate (KNO3), leaching duration, physical scarification as well as some environmental factors effective on seed germination such as salt and drought stresses, pH and seed planting depth. Seed germination was promoted with lower concentrations of KNO3 (0.01 to 0.02 g L-1), while higher concentrations reduced germination percentage. Seed dormancy was declined by low concentrations of GA3 up to 100 ppm. Seeds of greater bur-parsley germinated in a range of pH from 3 to 7. With enhancement of drought and salt stresses, seed germination decreased. Also, there was no seed germination in a high level of stresses. Seedling emergence reduced as planting depth increased. Use of GA3, KNO3, leaching and physical scarification had a positive effect on seed dormancy breaking of greater bur-parsley. The information from the study increases our knowledge about seed dormancy breaking techniques, response of germination to drought and salt stresses and also determination of distribution regions of greater bur-parsley in the future.

The effect of sodium hypochlorite, gibberellic acid, and light on seed dormancy and germination of wild buckwheat (Polygonum convolvulus) and cow cockle (Saponaria vaccaria)

1979 ◽  
Vol 57 (16) ◽  
pp. 1735-1739 ◽  
Author(s):  
A. I. Hsiao
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Sodium Hypochlorite ◽  
Seed Coat ◽  
Inhibitory Effect ◽  
Promoting Effect ◽  
Wild Buckwheat ◽  
Polygonum Convolvulus ◽  
Effect Of Light ◽  
Main Factor

Seed germination of wild buckwheat (Polygonum convolvulus L.) and cow cockle (Saponaria vaccaria L.) increased with increasing time of immersion in 6% sodium hypochlorite (NaOCl). Maximum germination was obtained at 6 to 8 h for wild buckwheat and at 2 h for cow cockle. The effect of NaOCl treatment of wild buckwheat seeds mimics the effect of acid scarification. Wild buckwheat germination was not influenced by light and (or) gibberellic acid (GA3). To induce 50% germination (t½) of cow cockle NaOCl treatments of 0.5 and 1 h were required for seeds incubating in the dark and light, respectively. Once the seed coat was made more permeable by NaOCl, both the promoting effect of GA3 and the inhibitory effect of light were increased. When the optimum effect of NaOCl occurred, all the seeds germinated. However, prolonged NaOCl treatment resulted in either poor germination or seed disintegration.The hard coat seems to be the main factor in regulating wild buckwheat seed germination. Cow cockle, however, is regulated by at least two other factors, light and hormones, in addition to seed coat.

scholarly journals Improving Dormancy and Germination of Piquín Chili Pepper (Capsicum annuum var. glabriusculum) by Priming Techniques

2018 ◽  
Author(s):  
Maria Fernanda Quintero Castellanos ◽  
Oscar Guillen Castillo ◽  
Pablo Delgado Sánchez ◽  
José Marín Sánchez ◽  
Ana Isabel Guzmán Carrasco ◽  
...  
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Seedling Establishment ◽  
Osmotic Potential ◽  
Seedling Emergence ◽  
Chili Pepper ◽  
Basic Information ◽  
Negative Effect

The effects of different priming techniques were evaluated to improve the dormancy and germination of wild seeds of “Piquín” chili pepper. Three experiments were designed for pre-sowing treatment of seeds: a) chemical seeds digestion; b) halopriming (with K+ or NH4+ of NO3-, SO42- or Cl-) at different priming times (24, 48 or 72 h) and osmotic potential (-5, -10 or -15 atm) and c) previously selected halopriming (KNO3 and NH4NO3) + Gibberellic acid (GA3, at 100 or 200 ppm) were tested. Digestion treatments did show a negative effect on seed germination. Recommended values of osmotic potential (Ψs), to improve Piquín chili seed germination, must be between -10 and -15 atm (-1.0 and -1.5 MPa) and the priming time must be between 48 and 72 hours. Priming techniques can considerably reduce Capsaicinoids content on seeds, improve dormancy, seed germination performance, and increase the rate and uniformity of seedling establishment. KNO3 and secondly GA3 treatments may improve rapid and uniform germination and seedling emergence. The results provide basic information to develop guidelines for commercial establishment of Piquín pepper crops.

Germination, and Seedling and Rosette Development of Flodman Thistle (Cirsium flodmanii)

Weed Science ◽  
1984 ◽  
Vol 32 (6) ◽  
pp. 768-773 ◽  
Author(s):  
Robert G. Wilson ◽  
Melvin K. McCarty
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Water Potential ◽  
Seedling Emergence ◽  
Soil Surface ◽  
Plant Water ◽  
Germination Medium ◽  
Water Potentials ◽  
Average Plant ◽  
Osmotic Potentials

Maximum germination of flodman thistle [Cirsium flodmanii(Rydb.) Arthur ♯3CIRFL] occurred at alternating temperatures of 10 to 20 and 20 to 30 C. Seeds germinated (20%) in 1.7 M NaCl. Light was important in seed germination; however, its absence could be overcome by adding 8.6 × 10-5M GA3(gibberellic acid) to the germination medium. The greatest seedling emergence occurred when seeds were planted on the soil surface and was lower when seeds were buried at any depth in the soil. Osmotic potentials at −0.5 MPa and greater reduced germination, but 3% of the seeds germinated at −1.3 MPa. Flodman thistle seedlings tolerated average plant water potentials of −0.9 and −1.1 MPa, but they died when the water potential was decreased to −1.5 MPa. Flodman thistle seedlings 43 days old and with four true leaves resprouted after topgrowth removal. Regrowth was evident on 75% of the clipped plants 13 days after initial topgrowth removal. Flodman thistle rosettes spread more rapidly and rosette survival was enhanced more in a weed-free nursery than in a weedy pasture.

scholarly journals Seed germination of Paederotella pontica (Rupr. ex Boiss.) Kem-Nath.- rare endemic species of the Caucasus

2020 ◽  
Vol 24 ◽  
pp. 00077
Author(s):  
Nana Shakarishvili
Keyword(s):  
Seed Germination ◽  
Tetrazolium Salt ◽  
Cold Stratification ◽  
Dormancy Breaking ◽  
Treatment Results ◽  
Relict Species ◽  
The Caucasus ◽  
Narrow Endemic ◽  
Dry Storage ◽  
Rare Endemic

Paederotella pontica is a Colchis tertiary relict species, regional narrow endemic to the Caucasus, representative of an oligotypic genus. The aim of this study was to determine dormancy-breaking requirements and develop seed germination protocol for P. pontica. Freshly matured seeds of P. pontica are morphophysiologically dormant (MPD). Mean length of seed is 820 μm, linear embryo is fully differentiated, on average 625 μm long. Penetration of tetrazolium salt indicates the permeability of seed coat and high percentage of vital seeds in capsules. Prior to root emergence, the E:S ratio increased from 0.76 to 0.9. Effects of warm and cold stratification and gibberellic acid (GA3) on embryo growth and seed germination were studied under laboratory conditions. Since cold stratification is the only requirement for the loss of MPD, the longest embryo growth occurred during this treatment and GA3 promoted MPD loss, we concluded that P. pontica seeds have intermediate complex MPD. Based on the treatment results a germination protocol is proposed: 1. Dry storage at 20°C, 2 months; 2. Cold-wet stratification at 3°C, 3 months; 3. Germination at 20/15°C day/night. Under developed conditions germination is fast, synchronous and yields to 80%.

scholarly journals Seed germination and seedling growth of two Pseudobombax species (Malvaceae) with contrasting habitats from Brazilian Cerrado

2018 ◽  
pp. 1915-1925
Author(s):  
Clesnan Mendes-Rodrigues ◽  
Paulo Eugênio Oliveira ◽  
Marli Aparecida Ranal
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Underground Structure ◽  
Seedling Emergence ◽  
Dry Mass ◽  
Underground Structures ◽  
Germination Time ◽  
Central Brazil ◽  
Mean Germination Time ◽  
Contrasting Habitats

Pseudobombax tomentosum and P. longiflorum are common trees in the Cerrado region, but the former species is more common in forest edges while the later is present in open cerrado areas. This work aimed to investigate differences in seed germination and seedling growth in these species, from seed collected from Cerrado areas in Central Brazil. For this, a seed germination experiment was designed and included four replicates with 25 seeds per species; seeds were randomly distributed in the germination chamber. To evaluate initial seedling growth, seedlings height was measured up to 67 days after seedling emergence; besides, some of these seedlings were grown for biomass evaluation during nine months. Results showed that seeds of the two species had the same germinability (near 100%) and mean germination time (ca. 12 days). However, P. longiflorum showed a more spread seed germination through time, with higher values of coefficient of variation in germination time and uncertainty index; and lower values of synchronization than P. tomentosum. The two species showed basically the same growth pattern, but lower values for height of apical meristem, diameter of underground structures (mostly roots), dry mass of shoots, underground structure and total mass of seedlings in P. tomentosum were obtained, compared to P. longiflorum. Both species allocated more dry mass to underground structures in detriment of shoot. This probably allows resprouting behavior which prevents hydric stress and detrimental fire action typical of the open Cerrado areas.

Factors affecting germination of Myricagale seeds

1989 ◽  
Vol 19 (9) ◽  
pp. 1105-1109 ◽  
Author(s):  
Christa R. Schwintzer ◽  
Andrea Ostrofsky
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Nitrogen Fixing ◽  
Factors Affecting ◽  
Dry Storage ◽  
Wet Storage ◽  
Absolute Requirement ◽  
Percent Germination ◽  
Extended Exposure

Several factors affecting seed germination were investigated in Myricagale L., a nitrogen fixing shrub common on wet soils. Light was essential: seeds failed to germinate in the dark and required four 16-h photoperiods (one per day) for maximum germination. There was no absolute requirement for prechilling, but prechilling increased germination by 75% in 2-month-old seeds and by 164% in 4.5 year-old seeds. Leaching did not affect germination and 500 ppm gibberellic acid enhanced germination only 20%. The seeds were long-lived: there was no decrease in percent germination during 6 years of dry storage at 5 °C and germination remained high after 1 year of wet storage at 5 °C. Because M. gale seeds often germinate on water-worked substrates, the requirement for extended exposure to light for germination may serve as a mechanism to prevent unsuccessful germination of seeds that are reburied after brief exposure to light as sediments are worked by water.

Seed afterripening and germination photoinhibition in the genusCrocus(Iridaceae)

2015 ◽  
Vol 25 (3) ◽  
pp. 306-320 ◽  
Author(s):  
Evangelia Skourti ◽  
Costas A. Thanos
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Low Temperatures ◽  
Temperate Climate ◽  
Optimal Temperature ◽  
Continuous Darkness ◽  
Dry Storage ◽  
The Mediterranean ◽  
Germination Experiments ◽  
First Time

AbstractMediterranean characteristics are attributed to the genusCrocus, which is inadequately studied in terms of seed germination. An afterripening requirement is very common in environments with warm and dry periods, and photoinhibition has been detected in many angiosperms inhabiting dry and open areas. The effects of afterripening and light on seed germination were investigated for the first time in 23 native GreekCrocustaxa, collected from various localities with either a Mediterranean or a temperate climate. Germination experiments were conducted in continuous darkness and in light at the optimal temperature for each taxon, with both freshly collected and afterripened seeds; warm stratification (20°C, darkness) was also examined in 22 taxa. A number of selected taxa were additionally investigated with respect to afterripening outdoors, afterripening and warm stratification at higher temperatures (35 and 25°C, respectively), stratification at 20/10°C, dry storage at low temperatures, response to gibberellic acid and phenology of embryo growth. It was postulated that an afterripening requirement is a characteristic of the genusCrocus, and we found that it can be fulfilled in nature during the Mediterranean dry summer. Also, for the vast majority of the taxa, warm stratification and stratification at 20/10°C can both meet the afterripening requirement. Embryos of the taxa studied are underdeveloped and have to grow prior to germination. Intrageneric differences of seed germination were observed only towards light, with photoinhibition being predominant in taxa from drier environments.

scholarly journals Effect of storage conditions and sodium hypochlorite treatment on germination of Cucumis prophetarum (Cucurbitaceae) seeds from arid Arabian deserts

2021 ◽  
Author(s):  
Hatem Ahmed Shabana ◽  
Tamer Mahmoud ◽  
Sanjay Gairola ◽  
Asma Al Ketbi ◽  
Mariam Aljasmi ◽  
...  
Keyword(s):  
Seed Germination ◽  
United Arab Emirates ◽  
Sodium Hypochlorite ◽  
Room Temperature ◽  
Seedling Emergence ◽  
Seed Storage ◽  
Storage Conditions ◽  
Germination Percentage ◽  
Continuous Darkness ◽  
Temperature Requirements

Abstract Background This is the first study on seed germination of Cucumis prophetarum in the Arabian Peninsula. Light and temperature as the main environmental factors and seed storage conditions greatly affect the germination of many Cucurbitaceae species. C. prophetarum is the perennial prostrate with woody rootstocks that grow throughout the year in the arid Arabian deserts. We examined the effects of seed storage conditions and sterilization by sodium hypochlorite (NaOCL) on germination of C. prophetarum seeds. Matured, fresh and field stored seeds were collected in March of 2016 and 2017 from a population in the United Arab Emirates. Fresh and the stored seeds (field, freeze, and room temperature) were germinated at three temperature regimes (15/25, 20/30 and 25/35°C) in both continuous darkness and alternating 12 h light/12 h darkness. Results The fresh and freeze stored seeds had higher germination percentage than the field and room temperature stored seeds. High germination was recorded in both lower and moderate temperatures (15/25 and 20/30°C) in light conditions. Sterilization of the seeds by NaOCl had a negative effect on the speed and germination percentage under all storage conditions, and it changes light and temperature requirements of seeds for germination. Conclusions Germination of C. prophetarum is sensitive to incubation light and temperature as well as to the seed storage conditions. The germination ability of stored seeds indicates that seed storage behaviour of C. prophetarum is orthodox. Light and temperature requirements for seed germination reflects a suitable place, habitat, and time for seedling emergence of C. prophetarum. The effect of NaOCl treatment on the germination may be associated with concentration and exposure periods of seeds. Therefore, we suggest that when using NaOCl for seed sterilization or to stimulate the germination, caution must be taken for using its concentration and exposure time, especially for species with thin and soft seed coats like C. prophetarum. Further studies on seed germination ecology would help to understand better the adaptive strategy of the C. prophetarum in the arid desert environments.

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