scholarly journals Germination of Osmotically Primed Asparagus and Tomato Seeds after Storage up to Three Months

1994 ◽  
Vol 119 (3) ◽  
pp. 636-641 ◽  
Author(s):  
Paul L. Owen ◽  
Wallace G. Pill

The influence of two drying regimes and two storage temperatures of primed asparagus (Asparagus officinalis L.) and tomato (Lycopersicon esculentum Mill.) seeds on germination after storage up to 3 months was examined. Seeds of `Mary Washington' asparagus and `Ace 55' tomato primed in synthetic seawater (-1.0 MPa, 20C, 1 week, dark) were surface-dried at 20C and 50% relative humidity (RH) for 2 h (42% to 49% moisture) or dried-back at 20C and 32.5% RH for 48 h (moisture = 13% tomato and 22% asparagus). These and nonprimed seeds were stored in tight-lidded metal cans and heat-sealed plastic pouches at 4 or 20C for up to 3 months before germination at 20C. After 3-month storage, primed surface-dried asparagus seeds stored at 4C had greater germination percentage and rate than nonprimed seeds, surface-dried seeds stored at 20C, or primed dried-back seeds. Dried-back primed tomato seeds had higher germination percentage than surface-dried primed seeds after 2 or 3 months of storage, with storage temperature having no effect on germination perecentage or rate. In a further study, primed surface-dried and primed dried-back seeds stored at 4 or 20C for 1.5 months in sealed containers were germinated at 15, 25, or 35C under low (-0.05 MPa) or high osmotic stress (-0.4 MPa). Primed surface-dried asparagus seeds stored at 4C, compared to nonprimed seeds, surface-dried seed stored at 20C, or primed dried-back seeds, had greater germination percentage at 15 and 35C and low osmotic stress, and higher germination rate at 15 or 25C. Primed tomato seeds had greater germination percentage than nonprimed seeds only at 35C and low osmotic stress, and higher germination rate at 15 or 25C. Storage of primed tomato seeds at 4C rather than 20C increased germination rate at 15 or 25C, and increased germination percentage at 35C and low osmotic stress. For maximal seed viability and germination rate after 1.5 to 3 months of storage, primed asparagus and tomato seeds should be stored at 4C rather than 20C; however, asparagus seeds should be surface-dried, and tomato seeds should be dried-back.

HortScience ◽  
1991 ◽  
Vol 26 (9) ◽  
pp. 1160-1162 ◽  
Author(s):  
W.G. Pill ◽  
J.J. Frett ◽  
D.C. Morneau

Seeds of `Ace 55' tomato (Lycopersicon esculentum Mill.) and `Mary Washington' asparagus (Asparagus officinalis L.) were primed in -0.8 MPa (20C, 1 week, dark) polyethylene glycol 8000 (PEG), synthetic seawater (INO), or NaNO3. Primed seeds of both species had a higher percentage of germination than untreated seeds only at 10C in nonsaline (– 0.05 MPa) medium, while in saline medium (– 0.6 MPa) priming increased the percentage of germination of tomato seeds at 10 and 30C, and of primed asparagus seeds at 10 and 20C. Sodium nitrate was superior to PEG or IN0 for priming tomato seeds since it resulted in fewer days to 50% germination and higher final germination percentage in saline media at all temperatures. IN0 was a satisfactory alternative to PEG or NaNO3 for priming asparagus seeds since priming agent had little or no effect on germination. Seedling emergence from NaNO3-primed seeds of both species sown in a seedbed provided saline (– 0.39 MPa) irrigation was faster than from untreated dry-sown seeds. In the saline seedbed, priming increased final emergence percentage (FEP) from asparagus seeds, provided they were not subsequently dried, but had no effect on the percentage emergence of tomato seeds. Fluid-drilling primed or germinated seeds of either species enhanced seedling establishment in the saline seedbed by reducing time to 50% emergence and/or increasing FEP relative to primed, dried-b&k or untreated seeds.


1992 ◽  
Vol 2 (3) ◽  
pp. 378-381 ◽  
Author(s):  
Luis Hernández-Rivera ◽  
Robert Mullen ◽  
Marita Cantwell

Asparagus (Asparagus officinalis L.) spears (cv. UC 157) trimmed to 20 cm were cooled 0, 4, 8, 12, 16, and 20 hours after harvest and evaluated for resistance to shear at 5 and 10 cm above the cut end immediately after cooling and during storage at 0.5, 2.5, and 5C. Resistance to shear increased during cooling delays and with time in storage. Initial cooling delays and simulated marketing conditions (transfer to 15C for 1 day) were the principal causes of increased resistance to shear. A 4-hour cooling delay resulted in an average 40% increase in resistance to shear. Transfer to 15C for 1 day resulted in a greater increase in shear force in the rapidly cooled than in the delayed-cooled spears. Increases in resistance to shear during storage varied from 0% to 50% and depended on the storage temperature, time, and the initial cooling delay. Spears trimmed to a green base showed less increase in shear force after storage than did spears trimmed to a white base. The visual quality of asparagus stored for 14 days was similar (excellent) among spears from different storage temperatures and between green- and white-base spears. Storage quality after 24 days had decreased more in spears stored at 5C than at 2.5 or 0.5C, and more in the green-base than the white-base spears.


HortScience ◽  
1991 ◽  
Vol 26 (8) ◽  
pp. 1054-1057 ◽  
Author(s):  
W.J. Carpenter ◽  
G.J. Wilfret ◽  
J.A. Cornell

Gladiolus (G. grandiflorus) seed germination was light-independent, but temperature influenced the germination rate. Constant 20C promoted higher total germination (97%), fewer days (4.3) to 50% of final germination, and shorter span of days (4.8) between 10% and 90% germination than other constant temperatures, although similar results were achieved by alternating 12-h cycles of 20 to 25C. Total germination was unchanged after seed treatment for 7 days at 10 to -20C, but longer germination periods were required after treatments below -10C. Reducing seed moisture contents from 11.8% to 4.2% caused no reduction in total germination, but moisture contents below 6.6% delayed achieving 50% of final germination and extended the periods from 10% to 90% of germination. Temperature and relative humidity (RH) during storage were important in retaining seed viability, with RH having a larger effect. Smallest declines in total germination during 12 months of storage occurred at 11% and 33% RH at 15C. The statistical analysis estimated the optimum seed storage at 14C and 26% RH.


HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 782-785 ◽  
Author(s):  
Wayne A. Mackay

Seeds of Lupinus havardii Wats. and L. texensis Hook. were subjected to scarification, storage temperature (4 or 22 °C), and relative humidity (RH) treatments (11%, 23%, 52%, 75%, or 97% RH) for 12 months. Seed moisture increased as relative humidity increased with scarified seed having the greatest increase in seed moisture content regardless of storage temperature. For both species, the combination of seed scarification before storage, 75% RH, and 22 °C storage temperature resulted in a significant and rapid decline in germinability beginning at 4 months. Scarified L. texensis seed stored at 52% RH and 22 °C also exhibited a significant decline in germinability following 6 months storage. Seed of both species stored under all other conditions germinated similar to or higher than the initial germination rate after 12 months. These results clearly show that scarification can be performed before seed packaging as long as the seed packets are stored at ≤23% RH under 4 or 22 °C with no loss in germinability for at least 1 year.


2012 ◽  
Vol 76 (1) ◽  
pp. 119-126 ◽  
Author(s):  
Maryam Haghighi ◽  
Zahra Afifipour ◽  
Maryam Mozafarian

The Alleviation Effect of Silicon on Seed Germination and Seedling Growth of Tomato Under Salinity StressThis study was conducted to evaluate the effectiveness of silicon (Si) application under salinity levels on seed germination and growth characteristics of tomato seeds. A laboratory experiment was performed on completely randomized design with two levels of salinity (25 and 50 mM NaCl) and 2 concentration of Si (1 and 2 mM) with 4 replications. Germination percentage, germination rate, seedling shoot and root length, fresh and dry weight of seedling and mean germination time was measured. Seed germination ofLycopersicon esculentumL. was significantly affected by salinity levels, Si and their interaction. Germination characteristics of tomato seeds decreased drastically by increasing NaCl concentrations. However, 1 mM Si had positive effects on seed germination characteristics and improved germination percentage, germination rate and mean germination time. Si alleviated the harmful effect of salinity stress on tomato seed germination at almost all germination characteristics.


Jurnal Wasian ◽  
2016 ◽  
Vol 3 (2) ◽  
pp. 69
Author(s):  
Yulianti Bramasto ◽  
Nurheni Wijayanto ◽  
Iskandar Z Siregar ◽  
I.G.K. Tapa Darma

The procurement of good quality seeds for the development of mindi (Melia azedarach L.) in the community forest is absolutely necessary. The purpose of this research is to investigate the diversity of the viability and seedling vigor of mindi from different populations with various treatments of dormancy breaking. The sample materials were mindi seeds that were collected from 6 locations (6 seed lots) or seed sources which were located in the community forest of West Java. There are 10 treatments of dormancy breaking that were applied in this study. The experiment design was Randomized Complete Design (RAL). The breaking dormancy  was focused to the delignification of hard seed coat.  Results showed that the most effective dormancy breaking for mindi seed was soaking in consentrated Sulfuric Acid (H2SO4) as long as 30 to 45 minutes. The first count for germination of mindi was on the 16 day and the final count was on the 31 day.The average value of germination percentage (DB) for all seed sources was 40 %, while the highest which is 60% was mindi from Sumedang seed source. The highest value of germination rate that is 6,543%/etmal was seed  from Sumedangand seed from Gambung was the lowest (1,400 %/etmal). Keywords : community forest, Melia azedarach L., variation, viability, vigor.


2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Ekosari Roektiningroem ◽  
Purwanti Widhy

This research aims to to know the seed viability of the two dates on the market. It has been done from July to November 2014, in the laboratory of  Mathematics and Natural Science Faculty, Yogyakarta State University. The type of Dates seeds are Sayer (oval-cilindrical, ±3cm, dark orange brown/more light than another),  and Lulu (round, ±2cm, dark maroon reddish /more dark than Sayer’s). Viability test by germination testing; conducted on plastic tray with cotton sheet and fabric covered; fill with 100 grains of seeds. Repeated 4 times. Incubation at room temperature (±30oC). The parameters were seed fresh weight, seed dry weight, seed moisture, germination percentage and germination rate. The results were analyzed by compare mean - independent samples t test of SPSS 18’th. The results showed that the viability of both of Date’s seeds from local’s market (Sayer and Lulu) is high. Sayer’s have better viability than Lulu’s (due to the germination percentage and germination rate). Keywords: date seed, viability test, germination test


1996 ◽  
Vol 121 (5) ◽  
pp. 804-809 ◽  
Author(s):  
I. David van der Walt ◽  
Gail M. Littlejohn

The influence of storage temperature and humidity on pollen viability was studied in four Protea species. Pollen was stored at a range of temperatures and relative humidities for up to 1 year and tested for ability to germinate in vitro. Pollen of P. repens (L.) L. `Sneyd', P. eximia (Salisb. ex Knight) Fourcade `Fiery Duchess' and P. magnifica Link. clone T 84 07 05 stored at -196 °C and -14 to -18 °C retained a germination percentage as high as that of fresh pollen regardless of humidity. Humidity control became increasingly important at storage temperatures above 0 °C. The study showed that long-term storage of Protea pollen is not feasible at temperatures above 0 °C. The relationship between germinability and fluorochromasia (FCR) was studied during storage of `Sneyd' pollen. The correlations between FCR and germinability were found to be low and nonsignificant. Fifteen-month-old cryopreserved `Sneyd' pollen functioned in fertilization and seed set as effectively as fresh pollen.


HortScience ◽  
1991 ◽  
Vol 26 (9) ◽  
pp. 1158-1159 ◽  
Author(s):  
J.J. Frett ◽  
W.G. Pill ◽  
D.C. Morneau

Tomato (Lycopersicon esculentum Mill.) and asparagus (Asparagus officinalis L.) seeds were primed for 1 week in –0.8 MPa (20C, dark) polyethylene glycol 8000 (PEG), synthetic seawater (INO), or combinations of Ca+2, K+, or Na+ with Cl–, \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{NO}_{3}^{-}\) \end{document}, \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{H}_{2}\mathrm{PO}_{4}^{-}\) \end{document}, or \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{SO}_{4}^{-2}\) \end{document}) and then were placed in 0.5-fold Hoagland solution at 20C for germination. Priming did not affect percentage germination of tomato seeds; but asparagus germination increased from 85% in untreated seeds to 90% due to priming. All priming treatments reduced the time to 50% germination (G50) of both species but reduced the time between 10% and 90% germination (G10-90) of only tomato seeds. Salt solutions gave lower G50 values than PEG for tomato seeds but did not provide this advantage for asparagus seeds. The G50 and G10-90 of tomato seeds were lower after priming with nitrate salts than with other salts, but the reductions were greatest with sodium nitrate. The G50 of asparagus seeds was lowered with nitrate salts, but the G10-90 was unaffected.


HortScience ◽  
1992 ◽  
Vol 27 (9) ◽  
pp. 993-996 ◽  
Author(s):  
William J. Carpenter ◽  
Joseph F. Boucher

Light, temperature, relative humidity (RH), and GA3 affect vinca [Catharanthus roseus (L.) G. Don] seed storage and/or germination. GA3 failed to increase the germination percentage in darkness but significantly increased the percentage in continuous light. Similarly, GA3 treatment reduced both the number of days required to achieve 50% of the final germination percentage (T50) and the span between 10% and 90% germination (T90 — T10) for seeds in light, but not in darkness. Germination percentages were maximal and about equal at 25, 30, or 35C in darkness; germination was lowest below 25C. Germination T50 and T90 — T10 required the fewest days between 25 and 35C. Reducing seed moisture from 9.9% to 3.9% increased the T50 from 2.4 to 3.0 days but failed to change germination percentages. Germination percentage declined linearly as seed storage temperatures were reduced from 5 to — 20C, whereas days to T50 increased. Seed storage for 12 months without reduction in germination percentage was possible at 5C and 11%, 33%, or 52% RH, but storage at 75% or 95% RH for periods exceeding 1 month reduced germination. Seeds stored at 33% or 52% RH required fewer days to T50 than did seeds stored at 11%, 75%, or 95% RH. Chemical name used: gibberellic acid (GA3).


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