scholarly journals Day and Night Temperature Differential (DIF) or the Absence of Far-red Light Alters Cell Elongation in `Celebrity White' Petunia

2006 ◽  
Vol 131 (3) ◽  
pp. 309-312 ◽  
Author(s):  
Gary R. Bachman ◽  
Margaret J. McMahon
Keyword(s):  
Environmental Control ◽  
Red Light ◽  
Cortical Cell ◽  
Internode Length ◽  
Cell Length ◽  
Cell Diameter ◽  
Growth Responses ◽  
Night Temperature ◽  
Spectral Filters ◽  
Pith Tissue

`Celebrity White' hybrid petunia plants (Petunia ×hybrida Hort. Vilm-Andr.) were grown either in chambers constructed of CuSO4-filled panels acting as spectral filters removing the far-red light (-FR) or in environmental control chambers under temperature treatments of 24 °C day/18 °C night (+DIF) or 18 °C day/24 °C night (-DIF). Growth responses for plants grown under CuSO4 filter (-FR) or -DIF temperatures were similar in that both treatments resulted in decreased internode length, increased stem diameter, and decreased cell length and cell diameter in epidermal, cortical, and pith tissues. Reduced cortical cell length contributed the largest percentage to internode length reductions compared to epidermal and pith tissue for the -FR treatment while reductions in cell length of all three tissues contributed to internode reduction of -DIF-treated plants. Chlorophyll a increased for plants grown under -FR, but decreased for plants grown in -DIF when compared to the appropriate controls.

scholarly journals INFLUENCE OF END OF DAY FAR-RED LIGHT ON HEIGHT OF CHRYSANTHEMUM PLANTS UNDER CuSO4 SPECTRAL FILTERS

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 649a-649
Author(s):  
Nihal C. Rajapakse ◽  
Margaret J. McMahon ◽  
John W. Kelly
Keyword(s):  
Weight Reduction ◽  
Red Light ◽  
Plant Morphology ◽  
Dry Weight ◽  
Internode Length ◽  
Internode Elongation ◽  
Additional Mechanism ◽  
Spectral Filters ◽  
Reduced Height ◽  
Stem Internode

The response of `Bright Golden Anne' and `Spears' chrysanthemum plants to EOD-R or FR light was evaluated to determine the involvement of phytochrome in regulation of plant morphology under CuSO4 filters. Light transmitted through the CuSO4 filter significantly reduced height, internode length and stem dry weight of `BGA' and `Spears' chrysanthemum plants. However, the degree of response varied with the cultivar. Exposure to EOD-FR reversed the reduction of plant height, internode length and the stem dry weight caused by the light transmitted through CuSO4 filters to a level comparable with control plants. Exposure to EOD-FR did not significantly alter height and stem dry weight under control filter Exposure to EOD-R light reduced the height and stem dry weight of `BGA' plants grown under control filter but EOD-R had no effect under CuSO4 filters. In `Spears' plants, EOD-R caused stem dry weight reduction under control filters, but did not reduce stem or internode elongation. The results suggest phytochrome may be involved in controlling plant response under CuSO4 filters. However, there are evidence to indicate that an additional mechanism may be acting on stem/internode elongation.

Growth Response and Carbohydrate Status of Gibberellin-treated Spectral Filter-grown Chrysanthemum Plants

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 522c-522
Author(s):  
Anuradha Tatineni ◽  
Sonja L. Maki ◽  
Nihal C. Rajapakse
Keyword(s):  
Plant Height ◽  
Growth Regulation ◽  
Red Light ◽  
Sucrose Phosphate Synthase ◽  
Spectral Filter ◽  
Spectral Filters ◽  
Wide Range ◽  
Ornamental Crops ◽  
Carbohydrate Levels ◽  
Carbohydrate Status

Interest in the use of non- (or less) chemical methods to reduce the height of ornamental crops has increased tremendously. Manipulation of greenhouse light quality is one alternative for plant growth regulation. We have shown that eliminating far-red light from the greenhouse environment with liquid CuSO4 spectral filters is effective in reducing the height of a wide range of plants though plant carbohydrate status is also altered under CuSO4 filter. In previous studies, application of GA3 reversed both the reduction of plant height and carbohydrate status of CuSO4 spectral filter grown plants. It has been proposed that GAs enhance the activity of the enzyme sucrose phosphate synthase to regulate carbohydrate levels. In the present study the role of exogenously applied GA19, GA1, and GA3 in overcoming the reduction of plant height and carbohydrate levels was investigated. Chrysanthemum plants were treated weekly for 4 weeks with saturating doses of GA19, GA1 and GA3 (25 μg) or the growth retardants paclobutrazol and prohexadione. GA1 was also applied with paclobutrazol and prohexadione to assess whether response to GAs is altered under CuSO4 filter. GA1 and GA3 promoted growth similarly under control or CuSO4 filter. GA19 was least effective in promoting growth under CuSO4 filter. In summary, these results suggest that gibberellin physiology is altered under spectral filters with the conversion of GA19 a possible point of regulation. The correlation between the carbohydrate status and the growth of the plants will be discussed.

INTERACTION OF TEMPERATURE AND CO2 ENRICHMENT ON SOYBEAN: GROWTH AND DRY MATTER PARTITIONING

1987 ◽  
Vol 67 (1) ◽  
pp. 59-67 ◽  
Author(s):  
NASSER SIONIT ◽  
B. R. STRAIN ◽  
E. P. FLINT
Keyword(s):  
Low Temperature ◽  
Environmental Control ◽  
Weight Ratio ◽  
Controlled Environment ◽  
Plant Responses ◽  
Growth Responses ◽  
Dry Matter Partitioning ◽  
Leaf Weight ◽  
Temperature Regimes ◽  
Increasing Temperature

Projected increases in atmospheric CO2 concentration will affect growth and productivity of many plant species under various environmental conditions. Since these increases in CO2 may also increase mean annual temperatures, it is important to determine how the soybean (Glycine max (L.) Merr.) will respond to changes in temperature regimes associated with atmospheric CO2 enrichment. Morphology and growth responses of the Ransom cultivar, which is adapted to a southern U.S.A. climate, to day/night temperature regimes of 18/12, 22/16, and 26/20 °C and atmospheric CO2 concentrations of 350, 675 and 1000 μL L−1 were studied in controlled environment chambers. Plant responses were determined at 20, 40, 67 and 115 (late senescence to maturity) days after planting. Plant height and number of branches increased slightly with CO2 enrichment and more significantly with increasing temperature. Root to shoot ratio remained unchanged at different CO2 concentrations but decreased as temperature increased. Leaf weight ratio and specific leaf weight decreased with increasing temperature. Low temperature reduced dry weight of all plant parts, but the reduction was ameliorated by increasing atmospheric CO2 concentration. The results show that increasing the atmospheric CO2 level causes soybean to grow more vigorously at low temperatures. Although controlled environment experiments have their drawbacks in regard to natural field conditions, the present data indicate that soybean will have enhanced growth even at moderately cool temperatures in the future global CO2 concentrations.Key words: Soybean growth, low temperature, CO2 × temperature interaction, environmental control

Environmental Control of Flowering of Boronia megastigma (Rutaceae) and Hypocalymma angustifolium (Myrtaceae)

1994 ◽  
Vol 42 (2) ◽  
pp. 219 ◽  
Author(s):  
JS Day ◽  
BR Loveys ◽  
D Aspinall
Keyword(s):  
Temperature Regime ◽  
Flower Development ◽  
Vegetative Growth ◽  
Environmental Control ◽  
Night Temperature ◽  
Root Temperature ◽  
Aerial Parts ◽  
Temperature Regimes ◽  
Controlled Flowering

The flowering responses of Boronia megastigma Nees (Rutacae) and Hypocalymma angustifolium Endl. (Myrtaceae) to different photoperiod and temperature regimes were similar despite the fact that these species are from different families. No flowers reached anthesis in a temperature regime of 25°C day/17°C night but flowering of both species occurred in a cool temperatures (17°C day/9°C night). Photoperiod had no effect on flowering at the temperatures tested. Ten weeks of cool temperatures (17°C day/9°C night) were required for a maximum number of flowers to reach anthesis on H. angustifolium plants whereas B. megastigma plants required 15 weeks. Flower development in both species was inhibited by a large difference between day and night temperature (21°C day/5°C night) and promoted if the day/night difference was reversed (9°C day/17°C night). The temperature of the aerial parts of the plant controlled flowering, whereas vegetative growth was controlled by root temperature. Therefore, while a reduction in vegetative growth naturally coincides with the production of flowers, these events are not necessarily linked.

End of day far-red light reverses height reduction of chrysanthemum induced by CuSO4 spectral filters

1993 ◽  
Vol 53 (3) ◽  
pp. 249-259 ◽  
Author(s):  
Nihal C. Rajapakse ◽  
Margaret J. McMahon ◽  
John W. Kelly
Keyword(s):  
Red Light ◽  
Spectral Filters ◽  
Height Reduction

scholarly journals CARBOHYDRATE STATUS OF CUSO4 SPECTRAL FILTER-GROWN CHRYSANTHEMUM PLANTS

HortScience ◽  
1996 ◽  
Vol 31 (5) ◽  
pp. 745d-745
Author(s):  
Venkat K. Reddy ◽  
Nihal C. Rajapakse
Keyword(s):  
Plant Height ◽  
Transmitted Light ◽  
Internode Length ◽  
Spectral Filter ◽  
Water Control ◽  
Spectral Filters ◽  
Light Passing ◽  
Carbohydrate Levels ◽  
Carbohydrate Status ◽  
The Relationship

The response of `Bright Golden Anne' chrysanthemum plants grown under CuSO4 spectral filters to exogenous GA3 application was evaluated to determine the relationship between gibberellins (GAs) and carbohydrate levels. The CuSO4 filters removed far red (FR) wavelengths of light and increased red: far red (R:FR), blue: far red (B:FR), blue: red (B:R) ratios, and phytochrome photoequilibrium (Ø) values of transmitted light compared to water (control) filter. Plant height, internode length, and leaf and stem dry weights were significantly reduced by light passing through CuSO4 filters in spring and summer seasons. Weekly applications of exogenous GA3 reversed the reduction in height and internode length induced by CuSO4 filters. Plants grown under CuSO4 filters responded more to exogenous GA3 application with respect to height and internode length, suggesting that the sensitivity to GA was not lowered. Light passing through CuSO4 filters reduced the carbohydrate levels, but the response varied with the season. Weekly GA3 application increased the carbohydrate levels, but did not totally reverse the reduction in carbohydrate levels under the CuSO4 filters. Although GA3 application increased the carbohydrate levels partially in CuSO4 filter-grown plants, the inhibition of GAs may not be solely responsible for reduction of carbohydrate levels under CuSO4 filters, showing that exogenous GAs and carbohydrate levels are not well correlated under CuSO4 spectral filters.

scholarly journals INFLUENCE OF SPECTRAL FILTERS ON LEAF PIGMENTS AND ANATOMY OF `SPEARS' CHRYSANTHEMUM

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 648g-648
Author(s):  
Margaret J. McMahon ◽  
John W. Kelly
Keyword(s):  
Chlorophyll A ◽  
Unit Area ◽  
Red Light ◽  
Leaf Size ◽  
Blue Dye ◽  
Textile Dye ◽  
Chlorophyll B ◽  
Spectral Filtering ◽  
Spectral Filters ◽  
Β Carotene

`Spears' chrysanthemums were grown in chambers fitted with double-walled exolite filled with spectral filtering solutions: a blue textile dye that absorbed red light, CuSO4·5H2O that absorbed far-red light, and H2O that was spectrally non-selective (control). Leaves of `Spears' grown under CuSO4-filters had increased chlorophyll a (23%), chlorophyll b (26%), xanthophyll (22%), and β-carotene (24%) compared to plants grown under H2O or blue-dye filters. Ratios of total carotenoid: chlorophyll and chlorophyll a: chlorophyll b were not affected by filter. Individual leaf area was reduced 25% under CuSO4 filters compared to other filters. Stomates per unit area were not affected by filters, however stomates per leaf were reduced 25% under CuSO4 filters because of leaf size reduction. Stomate length and width were not affected by filter. Leaves from plants grown under CuSO4-filters had an internal structure resembling that of sun-type leaves. Other filters induced a shade-type leaf.

scholarly journals Controlling Height with Temperature

1998 ◽  
Vol 8 (4) ◽  
pp. 535-539 ◽  
Author(s):  
Robert Berghage
Keyword(s):  
Plant Height ◽  
Production Systems ◽  
Formation Rate ◽  
Internode Length ◽  
Temperature Management ◽  
Night Temperature ◽  
Greenhouse Production ◽  
Node Number ◽  
Height Control ◽  
Average Temperature

Temperature management has emerged as an important tool for plant height control in greenhouse production systems. This is particularly important in vegetable transplant production where chemical controls for plant height are limited or not legal. Plant height is a function of the number of nodes and the length of each internode, and both are strongly influenced by greenhouse temperatures. Node number, or formation rate, is primarily a function of the average greenhouse temperature, increasing as the average temperature increases. Internode length is strongly influenced by the relationship between the day and night temperature, commonly referred to as DIF (day temperature - night temperature). As DIF increases, so does internode length in most plant species studied. Although the nature and magnitude of temperature effects vary with species, cultivar, and environmental conditions, these two basic responses can be used to modify transplant growth. Although data are limited, controlling transplant height with temperature does not appear to adversely influence plant establishment or subsequent yield.

scholarly journals 263 Photoselective Films Influence Flowering of Photoperiodic Species

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 436E-436
Author(s):  
Teresa A. Cerny ◽  
Nihal C. Rajapakse ◽  
Ryu Oi
Keyword(s):  
Plant Species ◽  
Light Quality ◽  
Red Light ◽  
Photon Flux ◽  
Crop Species ◽  
Plastic Films ◽  
Spectral Filters ◽  
Short Day ◽  
Long Day ◽  
Air Temperatures

Growth chambers constructed from photoselective plastic films were used to investigate the effects of light quality on height manipulation and flowering of photoperiodic plant species. Three types of treatment films were used; control, a far-red light intercepting film (YXE-10) and a red light intercepting film (SXE-4). The red (600-700 nm):far-red (700-800 nm) ratios and phytochrome photoequilibrium estimates for the control, YXE-10 and SXE-4 films were 1.0 and 0.71, 1.5 and 0.77, and 0.71 and 0.67, respectively. The photosynthetic photon flux was adjusted to uniformity among chambers using neutral density filters. Spectral filters did not effect minimum and maximum air temperatures. Experiments were conducted using quantitative long day (Antirrhinum majus and Petunia × hybrida), quantitative short day (Zinnia elegans and Dendranthema × grandiflorum) and day-neutral (Rosa × hydrida) plant species under natural short-day conditions. Plants produced under the YXE-10 filters were significantly shorter than the control plants, while plants produced under the SXE-4 films had similar or increased height compared to the control plants. However, both height response and flowering times varied with the crop species. Flowering time of Rosa × hybrida plants was uniform among all treatments. Flowering of quantitative long-day plants was delayed by at least 10 days under the YXE-10 film and was most responsive to the filtered light. Flowering of quantitative short-day plants was delayed by 2 days under the YXE-10. Days to flower for plants produced under the SXE-4 film were similar to the control plants for all species tested.

Simultaneous measurement of Ca2+, contraction, and potential in cardiac myocytes

1990 ◽  
Vol 258 (2) ◽  
pp. H574-H586 ◽  
Author(s):  
H. A. Spurgeon ◽  
M. D. Stern ◽  
G. Baartz ◽  
S. Raffaeli ◽  
R. G. Hansford ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Cardiac Myocytes ◽  
Free Acid ◽  
Red Light ◽  
Cell Length ◽  
Bright Field Image ◽  
Acetoxymethyl Ester ◽  
Bright Field ◽  
On Line ◽  
Subcellular Compartmentation

A system is described that can simultaneously record cytosolic Ca2+ concentration ([Ca2+]i), cell length, and either membrane potential or current in single cardiac myocytes loaded with the fluorescent Ca2+ indicator indo-1. Fluorescence is excited by epi-illumination with 3.8-microsecond flashes of 350 +/- 5 nm light from a xenon arc. Indo-1 fluoresence is measured simultaneously in spectral windows of 391-434 nm and 457-507 nm, and the ratio of indo-1 emission in the two bands is computed as a measure of [Ca2+]i for each flash. With cells loaded with the permeant acetoxymethyl ester of indo-1, quantitation of [Ca2+]i is not precise, owing to subcellular compartmentation of indo-1; however, the instrument would allow full quantitation if indo-1 free acid was introduced by microinjection. Simultaneously, cell length is measured on-line from the bright-field image of the cell. Because fluorescence collection is time gated during the brief flash, and red light (650-750 nm) is used for the bright-field image, cell length and [Ca2+]i measurements are obtained simultaneously without cross talk. Membrane potential or current can be recorded simultaneously with indo-1 fluorescence and cell length via standard patch-clamping techniques.

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