scholarly journals Antifungal potential of purified 3-(4-isopropylstyryl)-5-methylcyclohex-2-enone from marine actinobacteria Streptomyces albus A18

2020 ◽  
Vol 7 (16) ◽  
pp. 153-163
Author(s):  
Mizaeir Mehtha Abdul Kader ◽  
Murugan Sambantham ◽  
Jothiprakasam Vinoth
Keyword(s):  
1H Nmr ◽  
Solvent System ◽  
Molecular Formula ◽  
Marine Actinobacteria ◽  
Mass Spectral ◽  
Drug Molecules ◽  
Streptomyces Albus ◽  
Isolated Compound ◽  
Novel Drug ◽  
Chloroform Methanol

Actinomycetes are known to produce potential secondary metabolites which comprise biological activity. The present work endeavor is to assess the fungicidal property of novel marine actinobacterial compound 3-(4-isopropylstyryl)-5-methylcyclohex-2-enone extracted and isolated from Streptomyces albus AC18. The crude compound was loaded on silica gel column and eluted with chloroform - methanol - water. The purity of isolated compound were analyzed by TLC using chloroform and methanol as the solvent system and verified by GC-MS. The purified compound structure was established from infrared, ultraviolet, 1H-NMR, 13C-NMR and mass spectral data. The chemical shift assignments for the aliphatic compound from 1H-NMR corresponds to molecular formula as C18H22O. The Bioassay-guided fraction leads to the isolation of compound, was identified as 3-(4-isopropylstyryl)-5-methylcyclohex-2-enone. Hence, this marine isolated S. albus AC18 actino-bacterial compound seem to be more efficient in its antifungal activity and acts as prominent reservoir for novel drug molecules en route for answering several fungal diseases.

ISOLATION, CHARACTERIZATION AND ANTIFERTILITY ACTIVITY OF THE ACTIVE MOIETY FROM THE STEM OF MUSA PARADISIACA L.

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (09) ◽  
pp. 49-54
Author(s):  
P Soni ◽  
◽  
A. A. Siddiqui ◽  
J Dwivedi ◽  
V Soni
Keyword(s):  
Estrogenic Activity ◽  
Solvent System ◽  
Albino Rats ◽  
Alcoholic Extract ◽  
Traditional Use ◽  
Mass Spectral ◽  
Musa Paradisiaca ◽  
Antiestrogenic Activity ◽  
Isolated Compound ◽  
Oral Acute Toxicity

Bioactive principles from the hyroalcoholic (50%) extract of Musa paradisiaca L. stem were isolated and characterized to evaluate antifertility activity in female albino rats. Oral acute toxicity study was done with crude extract for 24h. The hydro alcoholic extract of M. paradisiaca stem was subjected to silica gel column chromatography using gradient solvent system DCM: Eth, Mth: Eth and Mth., ten different fraction were collected. The yield of fraction Mu-HA- Mps was 580 mg, further fractionated for purification by using solvent DCM-MeOH (3:2) to yield compound Mu-HA-Mps (20.22 %w/w). Isolated compound Mu-HA-Mps was subjected to evaluation of its antifertility potential by antiovulatory and estrogenic activity. The isolated compound Mu-HA-Mps was found to exhibit significant antiovulatory and antiestrogenic activity at doses of 100 and 200 mg/kg body weight. Isolated compound Mu-HAMps was subjected to structure elucidation by UV, IR, NMR and MASS spectral analytical methods. The results of the present study provide evidence of anti-fertility activity of isolated compound Mu-HA-Mps as claimed in the traditional use. The hydroalcoholic extract of Musa paradisiaca L. furnished a compound whose structure was established as 4’-methoxy-7-hydroxyisoflavone on the basis of physical and spectral basis and could be a good source of drug for birth control.

ISOLATION, CHARACTERIZATION AND ANTIFERTILITY ACTIVITY OF THE ACTIVE MOIETY FROM THE BARK OF FICUS RACEMOSA L.

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (05) ◽  
pp. 25-30
Author(s):  
V. Soni ◽  
◽  
A. K. Jha ◽  
J. Dwivedi ◽  
P. Soni
Keyword(s):  
Estrogenic Activity ◽  
Solvent System ◽  
Albino Rats ◽  
Alcoholic Extract ◽  
Traditional Use ◽  
Mass Spectral ◽  
Antiestrogenic Activity ◽  
Ficus Racemosa ◽  
Isolated Compound ◽  
Oral Acute Toxicity

Bioactive principles from the hyroalcoholic (50%) extract of Ficus racemosa L. bark were isolated and characterized to evaluate antifertility activity in female albino rats. Oral acute toxicity study was done with crude extract for 24h. The hydro alcoholic extract of F. racemosa bark was subjected to silica gel column chromatography using gradient solvent system Hex:EA, EA: Eth, Eth:Mth and Mth., eight different fractions were collected. The yield of fraction Fr-HA-F5 was 490 mg, further fractionation for purification by using solvent ethyl acetate-methanol (3:2) yielded compound Fr-HA-F5a (20.22 %w/w). Isolated compound Fr-HA-F5a was subjected to evaluation of its antifertility potential by antiovulatory and estrogenic activity. The isolated compound Fr-HA-F5a was found to exhibit significant antiovulatory and antiestrogenic activity at doses of 100 and 200 mg/kg body weight. Isolated compound Fr-HAF5a was subjected to structure elucidation by UV, IR, NMR and MASS spectral analytical methods. The results of the present study provide the evidence of the anti-fertility activity of isolated compound Fr-HA-F5a as claimed in the traditional use. The hyroalcoholic extract of Ficus racemosa L. furnished a compound with structure established as 2, 2’,4’-trimethoxy-5,7,8-trihydroxy-isoflavone on the basis of physical and spectral basis and could be a good source of drug for birth control.

Computational and Experimental Binding Mechanism of DNA-drug Interactions

2019 ◽  
Vol 24 (32) ◽  
pp. 3739-3757 ◽  
Author(s):  
Chandrabose Selvaraj ◽  
Sanjeev K. Singh
Keyword(s):  
Small Molecules ◽  
Self Assembly ◽  
Genetic Material ◽  
Dna Interaction ◽  
Significant Feature ◽  
Drug Molecules ◽  
Potential Applications ◽  
Novel Drug ◽  
Groove Binders ◽  
Molecular Docking And Dynamics

Nucleic acid is the key unit and a predominant genetic material for interpreting the fundamental basis of genetic information in an organism and now it is used for the evolution of a novel group of therapeutics. To identify the potential impact on the biological science, it receives high recognition in therapeutic applications. Due to its selective recognition of molecular targets and pathways, DNA significantly imparts tremendous specificity of action. Examining the properties of DNA holds numerous advantages in assembly, interconnects, computational elements, along with potential applications of DNA self-assembly and scaffolding include nanoelectronics, biosensors, and programmable/autonomous molecular machines. The interaction of low molecular weight, small molecules with DNA is a significant feature in pharmacology. Based on the mode of binding mechanisms, small molecules are categorized as intercalators and groove binders having a significant role in target-based drug development. The understanding mechanism of drug-DNA interaction plays an important role in the development of novel drug molecules with more effective and lesser side effects. This article attempts to outline those interactions of drug-DNA with both experimental and computational advances, including ultraviolet (UV) -visible spectroscopy, fluorescent spectroscopy, circular dichroism, nuclear magnetic resonance (NMR), molecular docking and dynamics, and quantum mechanical applications.

scholarly journals Green Synthetic Protocol for (E)-1-Aryl-3-(2-morpholinoquinolin- 3-yl)prop-2-en-1-ones and Their Antimicrobial Activity

2019 ◽  
Vol 31 (9) ◽  
pp. 1895-1898
Author(s):  
Relangi Siva Subrahmanyam ◽  
Venkateswara Rao Anna
Keyword(s):  
Antimicrobial Activity ◽  
Spectral Data ◽  
1H Nmr ◽  
13C Nmr ◽  
Mass Spectral Data ◽  
In Vitro Antimicrobial Activity ◽  
Mass Spectral ◽  
Fungal Organisms

We report here an easy, efficient and green synthetic protocol for the (E)-1-aryl-3-(2-morpholinoquinolin-3-yl)prop-2-en-1-ones by the Claisen-Schmidt condensation of 2-morpholinoquinoline-3-carbaldehyde and different substituted acetophenones by using 1-butyl-3-methylimidazolium tetrafluoroborate (Bmim)BF4. The compounds were characterized by using 1H NMR, 13C NMR and mass spectral data and screened there in vitro antimicrobial activity against different bacterial and fungal organisms.

scholarly journals Application of Cryo-Electron Microscopy on Drug Discovery

2021 ◽  
Vol 27 (S1) ◽  
pp. 3250-3250
Author(s):  
Viswanath Vittaladevaram ◽  
Kranthi Kuruti
Keyword(s):  
Electron Microscopy ◽  
Protein Complexes ◽  
Lead Discovery ◽  
Biologically Relevant ◽  
Biological Targets ◽  
3 Dimensional ◽  
Drug Molecules ◽  
Cryo Electron Microscopy ◽  
Therapeutic Molecules ◽  
Novel Drug

AbstractThe key aspect for development of novel drug molecules is to perform structural determination of target molecule associated with its ligand. One such tool that provides insights towards structure of molecule is Cryo-electron microscopy which covers biological targets that are intractable. Examination of proteins can be carried out in native state, as the samples are frozen at -175 degree Celsius i.e. cryogenic temperatures. In addition to this, there were no limits for molecular and functional structures of proteins that can be imagined in 3-dimensional form. This includes ligands which unravel mechanisms that are biologically relevant. This will enable to better understand the mechanisms that are used for development of new therapeutics. Application of Cryo-electron microscopy is not limited to protein complexes and is considered as non-specific. Intervention of Cryo-EM would allow to analyse the structures and also able to dissect the interaction with therapeutic molecules. The study determines the usage of cryo-EM for providing resolutions that are acceptable for lead discovery. It also provides support for lead optimization and also for discovery of vaccines and therapeutics.

Synthesis and Luminescence Properties of Tris (8-Hydroxyquinoline) Iron Spindle-Like Structures at Room Temperature

2011 ◽  
Vol 391-392 ◽  
pp. 225-229 ◽  
Author(s):  
Qing Hong Kong ◽  
Hong Liu ◽  
Yun Long Zhang ◽  
Yong Sheng Yan
Keyword(s):  
Electron Microscopy ◽  
Room Temperature ◽  
Blue Shift ◽  
Solvent System ◽  
Iron Complex ◽  
Molecular Formula ◽  
Gravimetric Analysis ◽  
Element Analysis ◽  
Transmission Electron ◽  
Fourier Transformation Infrared Spectroscopy

Spindle-like bis (8-hydroxyquinoline) iron (FeQ3) complex has been synthesized with a facile method in a mixed solvent system at room temperature for 12 h. The molecular formula of the products is speculated by the C, H and N element analysis and thermal gravimetric analysis, and Fourier-transformation infrared spectroscopy was also utilized to measure its structure, which further confirm the molecular formula of the products. The observation of field emission scanning electron microscopy and transmission electron microscopy shows that the morphology of tris (8-hydroxyquinoline) iron complex is spindle-like structure. The photoluminescence of the products were also investigated. The results indicate that the photoluminescence emission of FeQ3spindles shows obvious blue shift contrasted with that of 8-hydroxyquinoline.

Nanoparticulate Drug Delivery Systems: An update

2021 ◽  
pp. 312-316
Author(s):  
Pandey Swarnima ◽  
Sushant Kumar
Keyword(s):  
Drug Delivery ◽  
Carrying Capacity ◽  
Drug Delivery Systems ◽  
High Specificity ◽  
Delivery Systems ◽  
Classification Methods ◽  
Hydrophobic Drug ◽  
Drug Molecules ◽  
Conventional Drug ◽  
Novel Drug

The paper is aimed to provide a comprehensive review on nanoparticles, methods of preparation, applications in drug delivery. In recent years, there has been an exponential interest within the development of novel drug delivery systems using nanoparticles. Nanoparticles offers significant advantages over the conventional drug delivery in terms of high stability, high specificity, high drug carrying capacity, ability for controlled release, possibility to use in several route of administration and therefore the capability to deliver both hydrophilic and hydrophobic drug molecules. This review focuses on classification, methods of preparation, characterization, application, advantages of nanoparticles and health perspectives.

Phytopharmacognostical and Chromatographic Evaluation of Leaves of Launaea procumbens

2021 ◽  
pp. 4291-4299
Author(s):  
Brijesh Charadva ◽  
Urvashi Ghataliya ◽  
Pooja Meena ◽  
Krishna Karia ◽  
Tixa Lakhlani ◽  
...  
Keyword(s):  
Solvent System ◽  
Acid Anhydride ◽  
Phase System ◽  
Phytochemical Screening ◽  
Calcium Oxalate Crystal ◽  
Quantitative Microscopy ◽  
Qualitative And Quantitative ◽  
Anomocytic Stomata ◽  
Chloroform Methanol ◽  
Launaea Procumbens

Launaea procumbens leaves are galactagogue, diuretic, antifungal, anorexic, anti-arthritic and hepatoprotective according to Ayurvedic texts as well as modern research. Launaea species, particularly L. pinnatifida, is mentioned as one of the sources of Gojihva, regarding which there is controversy with respect to its true botanical identity. However, no detailed anatomical, phytochemical or chromatographic investigation is available for leaf of L. procumbens which can differentiate it from L. pinnatifida. The aim of this work is to develop standardization parameters of L. procumbens leaves by performing its pharmacognostical evaluation, preliminary phytochemical screening, HPTLC and GC-MS fingerprints. Pharmacognostic investigation of the leaves was performed by its morphological study, qualitative and quantitative microscopy as well as powder microscopy. Extraction of leaves was done by maceration using methanol. This extract was used for preliminary phytochemical screening and chemoprofiling by GC-MS, as well as for developing its HPTLC fingerprint. A mobile phase system was developed by pilot TLC, following which an HPTLC fingerprint was performed using the solvent system chloroform: methanol: ethyl acetate (3:7:6). Diagnostic microscopic characters identified in powder include unicellular covering trichome, xylem vessels, anomocytic stomata, and prisms of calcium oxalate crystal. Phytochemical screening revealed the presence of phytoconstituents classes like phenolics, anthraquinones, saponin glycosides, carbohydrates, sterols, triterpenoids and flavonoids. HPTLC fingerprinting detected 6 peaks with Rf 0.25, 0.37, 0.41, 0.50, 0.65, 0.78 at 254nm and 4 peaks with Rf 0.32, 0.37, 0.50, 0.66 at 366nm. GC-MS fingerprint revealed presence of propanoic acid anhydride, valeric anhydride, 2- Pyrrolidine acetic acid, phthalan, 5- (Hydroxy methyl)-2-(dimethoxy methyl) furan, vanillin, methyl β-l-Arabino pyranoside, 1,6-anhydro-β-D-Gluco pyranose and 6-Methyl-2-Heptanone, 6-(3,5-dimethyl-2-furanyl). Present study will be very useful for herbal industry in differentiating Launaea procumbens from other species of the genus Launaea, particularly L. pinnatifida (Gojihva) as well as for authentication, standardization and detection of adulteration in the leaf formulations of Launaea procumbens.

scholarly journals ISOLATION OF 2-CHLOROBENZIMIDAZOLE FROM MELIA DUBIA LEAF EXTRACT AND ITS STRUCTURAL CHARACTERISATION

2017 ◽  
Vol 9 (10) ◽  
pp. 67 ◽  
Author(s):  
G. Dayana Jeyaleela ◽  
S. Irudaya Monisha ◽  
J. Rosaline Vimala ◽  
A. Anitha Immaculate
Keyword(s):  
Mass Spectrometry ◽  
1H Nmr ◽  
Research Work ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Ft Ir ◽  
Uv Visible ◽  
Isolated Compound ◽  
Promising Source

Objective: Natural products from medicinal plants, either as isolated compounds or as standardized plant extracts exhibit promising source of medicinal activity against various diseases. The aim of the present work was to make an attempt of isolation of bioactive principle and characterization of the isolated compound, from the medicinal plant Melia dubaiMethods: The extraction was done by a cold percolation method and the compound was separated and isolated by chromatography technique such as a thin layer chromatography (TLC), column chromatography and high-performance liquid chromatography (HPLC). The isolated compound was crystallized and the structural characterization of the isolated compound was made using UV-Visible, FT-IR, 1H-NMR, GC-MS and MS techniques which confirmed the structure of the isolated compound.Results: The separated and isolated compound was characterized by both physical and spectral methods like Ultraviolet-Visible spectroscopy (UV-Visible), Fourier transform infrared spectroscopy (FT-IR), Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR), Gas chromatography-mass spectrometry (GC-MS), and Mass spectrometry(MS). Based on the studies, organizational characteristics of one bioactive principle were deciphered. The results revealed that the isolated species is 2-chlorobenzimidazole and it agreed well with the reported value and spectra for 2-chlorobenzimidazole.Conclusion: The above results obtained in this research work clearly indicated the promising occurrence of 2-chlorobenzimidazole in Media dubia plant leaves. The future scope of these studies may guide us to view the biological activity of the isolated compound.

scholarly journals Screening for antiparasitic activity of crude extracts of Pleurotus highking, a Bangladeshi edible mushroom

2015 ◽  
Vol 18 (1) ◽  
pp. 38-41 ◽  
Author(s):  
Md Anwarul Haque ◽  
Ashish Kumar Sarker ◽  
Ratan Kumar Paul ◽  
Sultana Shakila Khan ◽  
Md Anwar Ul Islam
Keyword(s):  
Methanolic Extract ◽  
Edible Mushroom ◽  
Pharmaceutical Journal ◽  
Dependent Manner ◽  
Reference Standard ◽  
Antiparasitic Activity ◽  
Drug Molecules ◽  
Percolation Method ◽  
And Control ◽  
Novel Drug

Compounds obtained from natural sources play significant role to identify various novel drug molecules. This study was designed to investigate parasitic susceptibility of methanolic extract from the Pleurotus highking, an edible mushroom commercially cultivated in Bangladesh against Pheretima posthuma. Extraction was carried out by continuous hot percolation method using methanol as a solvent. Four concentrations (10, 20, 40 and 80 mg/ml) of the extract were used for screening and results were expressed in terms of the time paralysis and death of worms. The extract exhibited promising antiparasitic activity at the concentration of 80 mg/ml. Albendazole and distilled water were used as reference standard and control, respectively. The extract showed its activity in the dose and time dependent manner. This is the first report of the antiparasitic activity of methanolic extract of P. highking.Bangladesh Pharmaceutical Journal 18(1): 38-41, 2015

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