ANTIBACTERIAL ACTIVITY OF BIOSYNTHESIZED ZnO NANOPARTICLES AGAINST GRAM POSITIVE (STAPHYLOCOCCUS AUREUS) AND GRAM NEGATIVE (PSEUDOMONAS AERUGINOSA) BACTERIA: A COMPARATIVE STUDY

Background. The microbiological risk of the hospital environment, including inert surfaces, medical devices, and equipment, represents a real problem. Objective. This study is aimed at demonstrating and assessing the antibacterial activity of three synthetic disinfectants classified as quaternary ammoniums on different bacterial strains (Gram-negative and Gram-positive like Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Pseudomonas aeruginosa, Acinetobacter baumannii, and Staphylococcus aureus) isolated from the hospital environment. The reference strains included Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, and Pseudomonas aeruginosa ATCC 27853 used as negative control strains. Method. Three quaternary ammonium disinfectants were tested: DDN9® (0.5%) which contains didecylmethylpolyoxyethylammonium propionate as an active substance, spray (0.4%) containing quaternary ammonium compounds, and Phagosurf ND® (0.4%) with didecyldimethylammonium chloride. Their effect was evaluated using the disk diffusion technique and the broth dilution methods, allowing the Minimum Inhibitory Concentration (MIC) and then the Minimum Bactericidal Concentration (MBC). Result. Only the growth of Gram-positive bacteria and some strains of Gram-negative bacteria were inhibited by the three synthetic disinfectants. NDD9® demonstrated an antibacterial effect only against the Gram-positive strains (S. aureus and S. aureus ATCC 29213) with a MIC of 0.25 mg/ml. The disinfectant spray showed effect against all four strains including E. coli (9), S. aureus, E. coli ATCC 25922, and P. aeruginosa ATCC 27853 with an inhibitory concentration of 4 mg/ml, while the growth of S. aureus ATCC 29213 was inhibited at 2 mg/ml. The third disinfectant, Phagosurf ND®, inhibited only the growth of S. aureus ATCC 29213 at a MIC of 4 mg/ml. Conclusion. This study is the first here in Morocco to evaluate the bacterial activity of products intended for the control of the healthcare environment. The results obtained on the three disinfectants tested reveal an ineffectiveness against some isolated strains from the hospital environment.

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UJI DAYA ANTIBAKTERI BAWANG PUTIH (Allium sativum L.) TERHADAP BAKTERI Staphylococcus aureus, Escherichia coli, Salmonella typhimurium DAN Pseudomonas aeruginosa DALAM MENINGKATKAN KEAMANAN PANGAN

Informatika Pertanian ◽

10.21082/ip.v24n1.2015.p53-58 ◽

2015 ◽

Vol 24 (1) ◽

pp. 53 ◽

Cited By ~ 2

Author(s):

Sri Suryatmiati Prihandani

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Food Safety ◽

Salmonella Typhimurium ◽

Diffusion Method ◽

Gram Positive ◽

Gram Negative ◽

Garlic Powder

Garlic is widely used as a herbal medicine. In order to support the empirical use and improve its application in food safety, this study was conducted to test the antibacterial activity of garlic against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli, Salmonella typhimurium and Pseudomonas aeruginosa) bacteria. Garlic was used in the form of fine powder. Garlic powder then dissolved in sterile distilled water and squeezed to obtain garlic solution with a concentration of 50%, 25% and 12.5%. Antibacterial activity of garlic juice was tested by diffusion method using paper disc to determine diameter of bacterial growth inhibition zones. Screening results demonstrated that the chemical constituents of garlic powder were saponins, flavonoids and triterpenoids. Garlic powder has antibacterial activity to Gram-positive S. aureus and Gram-negative bacteria E. coli, S. typhimurium and P. aeruginosa. Garlic has antimicrobial potential and can be used as decontaminant against Escherichia coli to maintain quality of food safety such as meat.

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Chemical Composition and Antibacterial Activity of the Essential Oil of Espeletia nana

Natural Product Communications ◽

10.1177/1934578x1200700530 ◽

2012 ◽

Vol 7 (5) ◽

pp. 1934578X1200700 ◽

Cited By ~ 2

Author(s):

Alexis Peña ◽

Luis Rojas ◽

Rosa Aparicio ◽

Libia Alarcón ◽

José Gregorio Baptista ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Diffusion Method ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Disk Diffusion Method ◽

Gram Negative ◽

Gram Positive Bacteria

The essential oil of the leaves of Espeletia nana Cuatrec, obtained by hydrodistillation, was analyzed by GC-MS, which allowed the identification of 24 components, which made up 99.9% of the oil. The most abundant compounds were α-pinene (38.1%), β-pinene (17.2%), myrcene (15.0%), spathulenol (4.2%), bicyclogermacrene (4.0%), α-zingiberene (4.0%), and γhimachalene (3.7%). Antibacterial activity was tested against Gram-positive and Gram-negative bacteria using the agar disk diffusion method. Activity was observed only against Gram-positive bacteria. MIC values were determined for Staphylococcus aureus ATCC 25923(200 μg/mL) and Enterococcus faecalis ATCC 29212 (600 μg/mL).

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A New Prenylated Flavanoid with Antibacterial Activity from Propolis Collected in Egypt

Natural Product Communications ◽

10.1177/1934578x1000500111 ◽

2010 ◽

Vol 5 (1) ◽

pp. 1934578X1000500 ◽

Cited By ~ 5

Author(s):

Ashraf El-Bassuony ◽

Sameh AbouZid

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bacillus Cereus ◽

Spectroscopic Methods ◽

Gram Positive ◽

Gram Negative

A novel prenylated flavanoid, isonymphaeol-D (1), together with two known compounds, isonymphaeol-B (2) and nymphaeol-B (3), were isolated from Egyptian propolis. The structures of the isolated compounds were determined by various spectroscopic methods. 1 exhibited antibacterial activity against Gram-positive (Bacillus cereus, Staphylococcus aureus) and Gram-negative strains (Serratia sp., Pseudomonos sp., Escherichia coli).

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Antibacterial Activity of Amine-Functionalized Zeolite NaY against Staphylococcus aureus ATCC6538 and Escherichia coli ATCC11229

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.761.402 ◽

2015 ◽

Vol 761 ◽

pp. 402-406 ◽

Cited By ~ 3

Author(s):

Siti Aishah Mohd Hanim ◽

Nik Ahmad Nizam Nik Malek ◽

Zaharah Ibrahim ◽

Mashitah Mad Salim ◽

Nur Isti'anah Ramli ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Inhibition Zone ◽

Antibacterial Activities ◽

Gram Positive ◽

Gram Negative ◽

Zeolite Nay ◽

Amine Functionalized ◽

Diffusion Technique

The antibacterial activity of functionalized zeolite NaY (CBV100) with different concentrations of 3-aminopropyltriethoxysilane (APTES) (0.01, 0.05, 0.20 and 0.40 M) was studied against Staphylococcus aureus ATCC 6538 (Gram positive) and Escherichia coli ATCC 11229 (Gram negative) through disc diffusion technique (DDT). The characterization of functionalized zeolite NaY with fourier transform infrared (FTIR) spectroscopy indicated the attachment of APTES on zeolite NaY. Through DDT, the inhibition zone of functionalized zeolite NaY increased proportionally to the amount of the amine-functional group attached onto zeolite NaY. Functionalized zeolite NaY showed higher antibacterial activity against Gram-positive compared to Gram-negative bacteria. It can be concluded from this study that amine-functionalized zeolite NaY shows evidence of antibacterial activities.

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COMPARATIVE STUDY OF ANTIBACTERIAL ACTIVITY OF LEAF EXTRACT OF GUAVA WITH GENTAMYCIN AGAINST GRAM POSITIVE AND GRAM NEGATIVE BACTERIA

World Journal of Pharmaceutical Research ◽

10.20959/wjpr201710-9319 ◽

2017 ◽

pp. 675-681

Author(s):

S. B. Chaudhari

Keyword(s):

Antibacterial Activity ◽

Comparative Study ◽

Leaf Extract ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Gram Negative

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Studies on the antibacterial properties of some basic derivatives of cholane and norcholane

Proceedings of the Royal Society of London Series B Biological Sciences ◽

10.1098/rspb.1947.0030 ◽

1947 ◽

Vol 134 (877) ◽

pp. 538-543 ◽

Cited By ~ 2

Keyword(s):

Staphylococcus Aureus ◽

Surface Tension ◽

Antibacterial Activity ◽

Antibacterial Properties ◽

Lactobacillus Helveticus ◽

Bacteriostatic Activity ◽

Gram Positive ◽

Gram Negative ◽

Derivatives Of

A series of basic derivatives of cholane and norcholane has been examined for bacteriostatic activity against the Gram-positive Staphylococcus aureus and Lactobacillus helveticus and the Gram-negative Bacillus lactis aerogenes . The basic derivatives, in general, were more highly bacteriostatic against the Gram-positive organisms than against the Gram-negative. Of the compounds studied, the highest antibacterial activity was shown by 3:7:12-trihydroxy-23-guanido-norcholane hydrochloride. No relationship was apparent between the lowering of the surface tension of the medium induced by the compounds and their bacteriostatic activity.

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Antimicrobial Biophotonic Treatment of Ampicillin-Resistant Pseudomonas aeruginosa with Hypericin and Ampicillin Cotreatment Followed by Orange Light

Pharmaceutics ◽

10.3390/pharmaceutics11120641 ◽

2019 ◽

Vol 11 (12) ◽

pp. 641 ◽

Cited By ~ 2

Author(s):

Seemi Tasnim Alam ◽

Tram Anh Ngoc Le ◽

Jin-Soo Park ◽

Hak Cheol Kwon ◽

Kyungsu Kang

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Cell Wall ◽

Gram Positive ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Wall Structures ◽

Global Issue ◽

Life Threatening ◽

Bacteria And Fungi

Bacterial antibiotic resistance is an alarming global issue that requires alternative antimicrobial methods to which there is no resistance. Antimicrobial photodynamic therapy (APDT) is a well-known method to combat this problem for many pathogens, especially Gram-positive bacteria and fungi. Hypericin and orange light APDT efficiently kill Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), and the yeast Candida albicans. Although Gram-positive bacteria and many fungi are readily killed with APDT, Gram-negative bacteria are difficult to kill due to their different cell wall structures. Pseudomonas aeruginosa is one of the most important opportunistic, life-threatening Gram-negative pathogens. However, it cannot be killed successfully by hypericin and orange light APDT. P. aeruginosa is ampicillin resistant, but we hypothesized that ampicillin could still damage the cell wall, which can promote photosensitizer uptake into Gram-negative cells. Using hypericin and ampicillin cotreatment followed by orange light, a significant reduction (3.4 log) in P. aeruginosa PAO1 was achieved. P. aeruginosa PAO1 inactivation and gut permeability improvement by APDT were successfully shown in a Caenorhabditis elegans model.

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Antibacterial Activity of Murrayaquinone A and 6-Methoxy-3,7-dimethyl-2,3-dihydro-1H-carbazole-1,4(9H)-dione

International Journal of Microbiology ◽

10.1155/2014/540208 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 4

Author(s):

Biswanath Chakraborty ◽

Suchandra Chakraborty ◽

Chandan Saha

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bacillus Subtilis ◽

Minimum Inhibitory Concentration ◽

Inhibitory Concentration ◽

Antibacterial Activities ◽

Gram Positive ◽

Gram Negative ◽

Naturally Occurring

The antibacterial activity of Murrayaquinone A (10), a naturally occurring carbazoloquinone alkaloid, and 6-methoxy-3,7-dimethyl-2,3-dihydro-1H-carbazole-1,4(9H)-dione (11), a synthetic carbazoloquinone, both obtained during the development of the synthesis of Carbazomycin G, having unique quinone moiety, was studied against Gram-positive (Bacillus subtilisandStaphylococcus aureus) and Gram-negative (Escherichia coliandPseudomonassp.) bacteria. Compound10showed antibacterial activities against both ofEscherichia coliandStaphylococcus aureuswhereas compound11indicated the activity againstStaphylococcus aureusonly. Both compounds10and11exhibited minimum inhibitory concentration (MIC) of 50 μg mL−1againstStaphylococcus aureus.

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Screening of antimicrobial, anti-quorum sensing activity and cytotoxicity of origanumoil against Gram-positive and Gram-negative bacteria

Biomedicine ◽

10.51248/.v41i3.1200 ◽

2021 ◽

Vol 41 (3) ◽

pp. 599-603

Author(s):

DevendraX Devendra Singh ◽

Vishnu Agarwal

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Quorum Sensing ◽

Klebsiella Pneumoniae ◽

Enterococcus Faecalis ◽

Inhibitory Zone ◽

Gram Positive ◽

Streptococcus Pneumonia ◽

Gram Negative ◽

Origanum Oil

Introduction and Aim: Essential oils have been used from ancient times to treat different gram-positive and gram-negative bacterial-related infections. The study aims to screen the antibacterial, anti-quorum sensing activity of origanum oil against the common infection causing gram-negative and gram-positive bacteria. Material andMethods: The MIC (minimum inhibitory concentration) and antibacterial activity of origanum oil against the eight bacterial species, namely, Pseudomonas aeruginosa, Shigella flexneri, Salmonella enterica, Klebsiella pneumoniae, Streptococcus pneumonia, Streptococcus pyogenes, Staphylococcus aureus, and Enterococcus faecalis, was assessed by broth-dilution method and well diffusion method. The anti-quorum sensing activity was analyzed using bioreporter strain CV026 at sub-MIC concentrations, while the cytotoxicity of the origanum oil was analyzed using the SW480 cell line. Results: The oil's antibacterial activity was analyzed by measuring the clear inhibitory zone diameter, and maximum inhibitory zone of 36.66 mm zone against Staphylococcus aureus (Gram-positive) and 33.33 mm against Salmonella enterica (Gram-negative) of origanum oil was measured. And the minimum inhibitory zone of 16.66 mm zone of Enterococcus faecalis (Gram-positive) and 19.66 mm against Klebsiella pneumoniae (Gram-negative) of oil was noted. And the lowest MIC (0.1 mg/ml) of oilwas found against Streptococcus pneumonia. The oil significantly inhibited the violacein pigment production (30.29 %) at 0.02 mg/ml concentration (this oil concentration did not significantly affect the growth curve). The in vitro cytotoxicity assay shows that the oil inhibited the SW480 cells growth with increasing concentration. Conclusion: The origanum oil possesses antibacterial and anti-quorum sensing activity and can be used as an alternative for treating tested bacterial infection.

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