scholarly journals Accelerated aging test and image analysis for barley seed

2019 ◽  
pp. 1546-1551
Author(s):  
Luciano Antônio Ebone ◽  
Inri Mateus Gonçalves ◽  
Nadia Canali Lângaro
Keyword(s):  
Digital Images ◽  
Seed Viability ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Barley Seed ◽  
High Yield ◽  
Germination Test ◽  
Hordeum Vulgare L ◽  
Barley Cultivars ◽  
Analysis System

Barley (Hordeum vulgare L.) planted area has increased in Brazil after a decline from 2005‒2009, but the country continues to import malt for the brewing industry. The physiological quality of the seed lot is essential for plant establishment and for screening for high yield grain. However, a uniform accelerated aging (AA) procedure has not been developed for testing barley seeds. Thus, this study aimed to determine the best AA method for measuring barley seed vigor. Seeds of three barley cultivars (BRS Cauê, BRS Brau, and MN 6021) were subjected to four AA treatment (T1: 41 °C for 72 h; T2: 42 °C for 48 h; T3: 42 °C for 60 h; and T4: 43 °C for 48 h). The following variables were evaluated using traditional vigor tests: seed viability in the first and last count of the germination test, number of seminal roots, shoot length, and radicle length. In addition, digital images of seedlings were acquired with the Seed Analysis System (SAS®) to compute seedling vigor, growth, and uniformity. AA methods T2 and T3 were effective in separating barley cultivars into vigor categories at day four of the germination test. Computer analysis of digital images of barley seedlings is a valuable tool for testing barley seed vigor. Accelerated aging methods T2 and T3 also gave the best separation in seed lot vigor and seedling growth using the SAS® index, respectively.

scholarly journals Digital images of seedling for evaluating coffee seed vigor

2019 ◽  
Vol 41 (1) ◽  
pp. 60-68 ◽  
Author(s):  
Heiber Andres Trujillo ◽  
Francisco Guilhien Gomes-Junior ◽  
Silvio Moure Cicero
Keyword(s):  
Seed Quality ◽  
Digital Images ◽  
Imaging System ◽  
Primary Root ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Control Programs ◽  
Scanned Images ◽  
Coffea Arabica L ◽  
Coffee Seed

Abstract: The digital image analysis of seedlings has become largely employed in seed quality-control programs due to its feasibility, objectivity and fast results. Despite these advantages, no studies have yet demonstrated the efficiency of this technique for analyzing coffee seed vigor. The present study aimed at evaluating the efficiency of the Seed Vigor Imaging System (SVIS®) in determining the vigor of coffee seeds (Coffea arabica L.) from digital images of seedlings. Also, the results of these analyses were contrasted with conventional vigor tests. Six seed lots from each cultivar, Bourbon and Catucaí 20/15, were used. The research was conducted in two experimental times, and the seed vigor was determined by the tests of first germination count, accelerated aging, electrical conductivity, primary root protrusion speed, as well as by the analysis of scanned images of seedlings, made possible by the SVIS® software. For the Bourbon and Catucaí 20/15 cultivars, the SVIS® analysis was able to discriminate lots of both high and low vigor (vigor indexes of 317 to 752, and 181 to 703, respectively). A similar outcome was obtained from the conventional tests appraised in this research. Eventually, it was possible to conclude that the digital analysis of 20-day-old seedlings by SVIS® is an efficient method to evaluate coffee seed vigor.

scholarly journals Tests for evaluating the physiological quality of pitaya seeds

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4047
Author(s):  
Thiago Alberto Ortiz ◽  
Aline Moritz ◽  
Lúcia Sadayo Assari Takahashi ◽  
Mariana Ragassi Urbano
Keyword(s):  
Electrical Conductivity ◽  
Pearson Correlation ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Water Volume ◽  
Germination Test ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.

scholarly journals 573 Seed Vigor Testing for Small-seeded Flower Species using Computer-aided Image Analysis

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 545C-545
Author(s):  
Kay Oakley ◽  
Robert Geneve ◽  
Sharon Kester ◽  
Patchara Wonprasaid
Keyword(s):  
Seed Quality ◽  
Digital Images ◽  
Accelerated Aging ◽  
Petri Dish ◽  
Seed Vigor ◽  
Radicle Growth ◽  
Agronomic Crops ◽  
Computer Aided ◽  
Initial Seed ◽  
Radicle Length

Standardized seed vigor tests must be developed for greenhouse-grown flower species. Current vigor tests used to evaluate large-seeded agronomic crops are generally not useful for evaluating smaller-seeded flower species. One alternative is to use radicle length in seedlings grown under controlled environments as an indicator of seed vigor. For that purpose, a seed vigor test was developed that uses digital images taken using a flat bed scanner to measure radicle length in small-seeded flower species. A novel, cellulose substrate was used for germinating seeds. It provided similar moisture-holding properties to standard germination blotters used by commercial seed analysts, but is clear. This has allowed for quick image acquisition without removing seedlings from the petri dish. Correlations were made between seedling growth (radicle length, total seedling length, and total seedling area) with other vigor tests (saturated salts accelerated aging) and greenhouse plug flat emergence. For several seed lots of impatiens that varied in initial seed quality, radicle length after 4 days showed good correlations (>R2 = 0.79) with other measures of seed vigor for describing seed quality. This system is an improvement over other attempts to use computer-aided assessment of digital images because it provides digital images that do not vary due to external lighting; it uses software that can evaluate radicle length in a petri dish assay that does not require a slant-board for straight radicle growth; it relies on standard germination technics used by every seed lab; it uses a clear substrate to replace the opaque blotter to allow digital images to be taken within the petri dish; and accurate measurements of seedling parts is performed in under 2 min per petri dish.

scholarly journals ELECTRICAL CONDUCTIVITY AND ETHANOL RELEASE TO ASSESS RED RICE SEED VIGOR1

2021 ◽  
Vol 34 (4) ◽  
pp. 791-798
Author(s):  
RAFAEL MARANI BARBOSA ◽  
MATHEUS ANDRÉ DE JESUS ◽  
RAFAELA ALVES PEREIRA ◽  
GEDEON ALMEIDA GOMES JUNIOR
Keyword(s):  
Electrical Conductivity ◽  
Efficient Method ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Rice Seed ◽  
Seed Sample ◽  
Red Rice ◽  
Germination Test ◽  
Rice Seeds

ABSTRACT To evaluate seed vigor, electrical conductivity and ethanol tests are fast and efficient methodologies. They have the potential to be used in several species, such as red rice. However, there are no protocols or information about their efficiency. Thus, the objective was to evaluate the efficiency, and define parameters of execution for electrical conductivity and ethanol tests, to evaluate the vigor of red rice seeds. The study was conducted using four lots of ‘BRS 901’ red rice, which was subjected to a germination test, as well as first count, accelerated aging, and field seedling emergence tests. The electrical conductivity test was performed with 25, 50, and 100 seeds soaked in 50 mL and 75 mL of water, at 25 °C and 30 °C, for 3, 6, 20, and 24 hours, respectively. The ethanol test was performed with 50 and 100 seeds soaked in a volume of water equivalent to 1.0, 1.5, 2.0, 2.5, and 3.0× the mass of the seed sample. To assess the vigor of red rice seeds, the electrical conductivity test is an efficient method when conducted with 50 seeds soaked in 50 mL of water at 25 °C for 20 hours. Meanwhile, the ethanol test is most effective when performed with 50 seeds, in a volume of water that is 2.5× the mass of the sample, at 40 °C for 24 hours.

scholarly journals Physiologic Quality and Biochemical Characters of Barley Seeds Produced Under Nitrogen Doses and Growing Environments

2019 ◽  
Vol 11 (12) ◽  
pp. 65
Author(s):  
Lanes B. A. Jaques ◽  
Ivan R. Carvalho ◽  
Vinícius J. Szareski ◽  
Henrique E. Rodrigues ◽  
Ítala T. P. Dubal ◽  
...  
Keyword(s):  
Block Design ◽  
Accelerated Aging ◽  
Dry Mass ◽  
Human Consumption ◽  
Limiting Factor ◽  
Hordeum Vulgare L ◽  
Barley Cultivars ◽  
Randomized Block Design ◽  
Physiological Quality

Barley (Hordeum vulgare L.) is considered one of the most important cereals, such as maize, rice and wheat, due to its multiplicity of usage, as much for human consumption as for feed. The Nitrogen is one of the main nutrients that influences the quality and productivity of barley seeds because it participates in numerous metabolic routes. It is a limiting factor of the production, the lack or the excess can bring consequence to the culture. In this manner, the present study aims to evaluate the influence of different nitrogen doses on the physiologic quality and biochemical characters of brewing barley seeds produced in two growing environments. The experiment was conducted in the 2017 crop season, in a randomized block design, organized in a factorial scheme, being 2 cultivation environment &times; 2 brewing barley cultivars &times; 4 nitrogen doses, disposed in four replicates. The physiological quality of the seeds was evaluated by germination, first counting of germination, accelerated aging, field emergence, shoot and root length, dry mass of shoot and root of seedlings, electrical conductivity (3, 6 and 24 hours) and isoenzymes. The growing environment promotes differences in the barley seeds physiologic quality. The nitrogen fertilizing, at dose of 120 kg ha-1, results in superiority in the physiologic quality of seeds. The cultivar BRS Cau&ecirc; in both environments presented higher vigor. The nitrogen fertilizing alters the isoenzymatic expression of barley seedlings, creating variation in the bands intensity, at different nitrogen doses.

scholarly journals Viability of soybean seed produced under different agro-meteorological conditions in Vojvodina

Genetika ◽  
2011 ◽  
Vol 43 (3) ◽  
pp. 625-638 ◽  
Author(s):  
Milka Vujakovic ◽  
Svetlana Balesevic-Tubic ◽  
Dusica Jovicic ◽  
Ksenija Taski-Ajdukovic ◽  
Dragana Petrovic ◽  
...  
Keyword(s):  
Laboratory Test ◽  
Seed Viability ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Cold Test ◽  
Seed Vigor ◽  
High Soil Moisture ◽  
Air Temperatures ◽  
Accelerated Aging Test ◽  
High Germination

At the time of soybean seed sowing in the field, a high soil moisture, low soil and air temperatures, and crasts formation may occur, which can lead to slow germination, poor seedling establishment, and in some cases to loss of seed vigor. Due to the importance and prevalence of soybean the aim of this study was to determine the quality and seed viability of different genotypes produced at three locations in Vojvodina during 2009 and 2010. Eight soybean varieties (Afrodita, Valjevka, Balkan, Novosadjanka, Ravnica, Ana, Vojvodjanka and Venera) produced in Vrbas, Senta and Indjija during 2009 and 2010 were tested. Seed germination was determined using Standard laboratory test, and vigor tests (cold test, and accelerated aging test). Studied genotypes baheved differently in different years and at different localities. Genotype Venera achieved high germination values in all applied tests in 2009, while genotype Afrodita had high values of the tested parameter when conventional laboratory test was applied, and the lowest values were recorded when vigor tests were applied. Values obtained in 2010 when all tests were applied were above the prescribed minimum. Locality of Vrbas proved to be more favorable for seed production in relation to localities of Indjija and Senta due to better rainfall distribution.

scholarly journals Controlled deterioration test for evaluation of sunn hemp seed vigor

2015 ◽  
Vol 37 (4) ◽  
pp. 249-253 ◽  
Author(s):  
Arthur Batoqui Costa e Silva ◽  
Ícaro Monteiro Galvão ◽  
Rafael Marani Barbosa ◽  
Clíssia Barboza da Silva ◽  
Roberval Daiton Vieira
Keyword(s):  
Moisture Content ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Germination Test ◽  
Seed Moisture Content ◽  
Hemp Seed ◽  
Seed Moisture ◽  
Sunn Hemp ◽  
Controlled Deterioration ◽  
Initial Seed

The controlled deterioration test is efficient for seed vigor evaluation of several species. However, the procedures conditions are incipient, especially in respect to tropical forages such as sunn hemp, which still does not have a standardized procedure. The objective of this research was to adjust the procedures for a controlled deterioration test in Crotalaria juncea L. seeds. For this, five commercial seed lots were evaluated for water content and initial physiological potential (germination test, first germination count, germination speed index, saturated salt accelerated aging with NaCl, electrical conductivity and seedling field emergence). For controlled deterioration test, the initial seed moisture content was adjusted to 18, 21 and 24% and, subsequently, the samples were exposed at 45 °C during 24 hours. Seed germination test was conducted with the use of sand as substrate. Evaluations were performed on the fourth, fifth and sixth day after sowing. The controlled deterioration test for sunn hemp seeds should be performed with 24% seed moisture content, at 45 °C during 24 hours, and the test evaluation on the fourth day after sowing.

scholarly journals O GRAU DE UMIDADE NA COLHEITA E O SISTEMA DE SECAGEM SÃO DETERMINANTES PARA O VIGOR DE SEMENTES DE ARROZ

2021 ◽  
Vol 36 (1) ◽  
pp. 28-40
Author(s):  
Jaquelini Garcia ◽  
Cileide Maria Medeiros Coelho
Keyword(s):  
Moisture Content ◽  
Seed Viability ◽  
Mechanical Damage ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Rice Seed ◽  
Randomized Design ◽  
Physiological Quality ◽  
Drying System

O GRAU DE UMIDADE NA COLHEITA E O SISTEMA DE SECAGEM SÃO DETERMINANTES PARA O VIGOR DE SEMENTES DE ARROZ   JAQUELINI GARCIA1, CILEIDE MARIA MEDEIROS COELHO1   1Programa de Pós-graduação em Produção vegetal, Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, 2090, Conta Dinheiro, 88520-000, Lages, SC, Brasil, [email protected], [email protected]   RESUMO: O grau de umidade na colheita e o sistema de secagem podem ser determinantes na qualidade das sementes produzidas. Neste trabalho foi avaliado se o sistema de secagem e o grau de umidade na colheita interferem na qualidade fisiológica das sementes de arroz. Foi realizado em delineamento inteiramente casualizado em esquema fatorial triplo. Foi utilizada seis cultivares produzidas no Alto Vale do Itajaí/SC na safra 2016/17 com grau de umidade na colheita igual ou superior a 17% (19,30 - 17,30%) e inferior a 17% (16,40 - 15,03%) (base úmida). As sementes foram submetidas aos sistemas de secagem estacionário e intermitente. Realizou-se testes de germinação, viabilidade, vigor pelo envelhecimento acelerado e frio. Sementes colhidas com grau de umidade ≥ 17% apresentaram maior vigor. A cultivar foi um fator de grande relevância na sensibilidade ao dano térmico e mecânico. O sistema de secagem não afetou a viabilidade das sementes, com exceção da cultivar SCS122 Miura que foi sensível a dano térmico e mecânico. A secagem estacionária comprometeu a qualidade fisiológica. Conclui-se que a secagem intermitente e a colheita com grau de umidade igual ou superior a 17%, mantém a qualidade fisiológica das cultivares SCSBRS Tio Taka, SCS122 Miura, SCS116 Satoru e SCS121 CL.   Palavras-chaves: Secagem estacionária, secagem intermitente, qualidade fisiológica.   THE DEGREE OF MOISTURE IN THE HARVEST AND THE DRYING SYSTEM ARE DETERMINANT FOR THE RICE SEED VIGOR   ABSTRACT: The degree of moisture at harvest and the drying system can determine the quality of the seeds produced. In this work, it was evaluated whether the drying system and the degree of moisture at harvest affect the physiological quality of rice seeds. It was conducted in a completely randomized design in a triple factorial scheme. Six cultivars produced in Alto Vale do Itajaí/SC in the 2016/17 crop were used, with moisture content at harvest equal or greater than 17% (19.30 – 17.30%) and less than 17% (16,40 - 15,03%) (wet basis). Seeds were submitted to stationary and intermittent drying systems. Tests of germination, viability, vigor by accelerated aging and cold were performed. Seeds harvested with moisture content ≥ 17% showed greater vigor. The cultivar was a factor of great relevance in the sensitivity to thermal and mechanical damage. The drying system did not affect seed viability, except for the cultivar SCS122 Miura, which was sensitive to thermal and mechanical damage. Stationary drying compromised physiological quality. It was concluded that intermittent drying and harvesting with moisture content equal or greater than 17% maintain the physiological quality of the SCSBRS Tio Taka, SCS122 Miura, SCS116 Satoru and SCS121 CL cultivars.   Keywords: Stationary drying, intermittent drying, physiological quality.

scholarly journals SAPL®: a free software for determining the physiological potential in soybean seeds1

2018 ◽  
Vol 48 (3) ◽  
pp. 222-228 ◽  
Author(s):  
André Dantas de Medeiros ◽  
Márcio Dias Pereira
Keyword(s):  
Seedling Emergence ◽  
Soybean Seed ◽  
Cold Test ◽  
Seed Vigor ◽  
Soybean Seeds ◽  
Germination Test ◽  
Vigor Index ◽  
Seedling Length ◽  
Powerful Approach ◽  
Analysis System

ABSTRACT Obtaining image-based information is a powerful approach to capture and quantify seed vigor data. However, commercial systems that facilitate the processing and acquisition of images are often cost prohibitive. This study aimed to evaluate the efficiency of the Seedling Analysis System (Sistema de Análise de Plântulas - SAPL®), in order to analyze the physiological potential of soybean seeds, in comparison with the information provided by vigor tests which are traditionally recommended for this species. Nine lots of soybean seeds were submitted to germination, germination speed, seedling emergence, cold test and electrical conductivity tests. In the SAPL® analyzes, seedlings of four and six days, counted from the beginning of the germination test, were evaluated, resulting in values for seedling length, growth, development uniformity, vigor index and corrected vigor index. The evaluated lots with emergence greater than 90 % showed a vigor index higher than 600 and 800, respectively in the fourth and sixth days. The indexes generated by SAPL®, except for the uniformity index, presented positive and high correlations with the traditional tests (> 0.80). SAPL® is efficient in identifying differences in the vigor of soybean seed lots.

scholarly journals Parameters of the Accelerated Aging Test for the Determination of Tobacco (Nicotiana tabacum L.) Seed Vigor

2020 ◽  
Vol 8 (1) ◽  
pp. 247
Author(s):  
Luis Henrique Konzen ◽  
Daniele Brandstetter Rodrigues ◽  
Alcimar Spindola Mazon ◽  
William Aldrei Guareschi ◽  
Jerffeson Araujo Cavalcante ◽  
...  
Keyword(s):  
Saline Solution ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Exposure Period ◽  
Seed Vigor ◽  
Germination Test ◽  
Accelerated Aging Test ◽  
Tobacco Seeds ◽  
Aging Test

Vigor tests are very important to obtain additional information to the standard germination test and can assist in decision-making. The aim of this work was to evaluate accelerated aging test methodologies for the determination of vigor in tobacco seeds. For the study, ten seed lots of tobacco were used. The initial quality of the tobacco seeds was determined through the germination test, first count of germination, germination speed index, seedling emergence at 7, 14 and 21 days after sowing, emergence speed index and the accelerated aging with water performed as proposed by the Association of Official Seed Analysts. After the determination of seed initial quality, the accelerated aging test was studied in the following methods: accelerated aging with water, saturated saline solution (40g NaCl 100mL-1 water) and unsaturated saline solution (11g NaCl 100mL-1 water), submitted to the temperatures of 45 and 41°C, for the exposure periods of 24, 48 and 72 hours. According to the results obtained, the accelerated aging test with water conducted under a temperature of 45°C combined with a 24-hour exposure period is shown as adequate for evaluating the vigor of tobacco seeds.

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