scholarly journals Identification and antimicrobial susceptibility of microbial agents of otitis externa in dogs

2022 ◽  
Vol 74 (10) ◽  
pp. 6136-2022
Author(s):  
SERPIL KAHYA DEMIRBILEK ◽  
ÖZGE YILMAZ
Keyword(s):  
Otitis Externa ◽  
Susceptibility Testing ◽  
Acinetobacter Calcoaceticus ◽  
Trichophyton Mentagrophytes ◽  
Polymyxin B ◽  
Malassezia Pachydermatis ◽  
Antimicrobial Sensitivity ◽  
Amoxicillin Clavulanic Acid ◽  
Staphylococcus Spp ◽  
Microbial Agents

In this study, a total of 277 unmedicated dogs with otitis externa were used. Overall, 413 agents were isolated from 277 ear swab samples; 52.7% of the cases were mono-infections (146 cases), and 37.1% of the cases were poly-infections (103 cases). In 10.1% (28) of the cases, neither bacteria nor yeasts were isolated. Coagulase-positive Staphylococcus spp. were the most frequently isolated bacteria and were found in 90 (21.8%) of the samples. Fifty-eight samples, (14%) were positive for Staphylococcus aureus, 51 (12.3%) for Pseudomonas aeruginosa, 27 (6.5%) for Proteus mirabilis, 27 (6.5%) for Malassezia pachydermatis, 21 (5%) for Corynebacterium spp., 21 (5%) for β-haemolytic Streptococcus spp., 15 (3.6%) for Staphylococcus pseudointermedius, 12 (2.9%) for Proteus spp., 12 (2.9%) for Escherichia coli, 9 (2.1%) for Acinetobacter calcoaceticus, 7 (1.6%) for Trichophyton mentagrophytes, 5 (1.2%) for Staphylococcus auricularis, and 46 (11.1%) for different bacteria and yeasts. A total of 14 different bacteria and yeasts were isolated and identified. Kirby-Bauer antibiotic susceptibility testing was carried out for 10 different antibiotics. The bacterial isolates were found to be resistant to amoxicillin-clavulanic acid (45%), gentamycin (28%), ampicillin/cloxacillin (69%), tobramycin (28%), amikacin (23%), enrofloxacin (47%), chloramphenicol (58%), doxycycline (65%), lincomycin/spectinomycin (58%) and polymyxin B (62%). In conclusion, it is important to test the antimicrobial sensitivity of aetiological agents of otitis externa before treatment so as to prevent the development of antibiotic resistance in bacteria and yeasts.

scholarly journals Microbiological and antibacterial resistance profile in canine otitis externa – a comparative analysis

2019 ◽  
Vol 22 (4) ◽  
pp. 447-456
Author(s):  
V. Petrov ◽  
G. Zhelev ◽  
P. Marutsov ◽  
K. Koev ◽  
S. Georgieva ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Otitis Externa ◽  
Bacterial Species ◽  
Antibacterial Resistance ◽  
Clear Trend ◽  
Malassezia Pachydermatis ◽  
Resistance Patterns ◽  
Amoxicillin Clavulanic Acid ◽  
Microbial Agents ◽  
Coagulase Positive Staphylococci

The aim of the present study was to compare the prevalence of microbial agents involved in canine otitis externa and their sensitivity to antibacterial drugs in two periods: 2007–2011 and 2013–2017. For 2013–2017, coagulase-positive staphylococci were the dominating bacterial species (186 isolates), followed by Pseudomonas aeruginosa (82 strains). The rate of isolated yeasts (mainly Malassezia pachydermatis) was substantially high (152 isolates). Compared to the earlier period (2007–2011) a tendency to more frequent occurrence of co-infections was noted – 61.7% and more than 80% of co-infections involved yeasts. Antibacterial resistance patterns showed a clear trend to increased resistance of coagulase-positive staphylococci and β-haemolytic streptococci to amoxicillin/clavulanic acid (42% and 50% respectively) and gentamicin (29%, 40%). Increased resistance of P. aeruginosa was established to gentamicin (16%) and amikacin (18%). The prevalence of pseudomonads resistant to enrofloxacin was lower (27%).

scholarly journals Phenotype characterization of Staphylococcus species strains isolated from buffalo (Bubalus bubalis) milk

2011 ◽  
Vol 23 (6) ◽  
pp. 1208-1211 ◽  
Author(s):  
Andrea A. F. Oliveira ◽  
José W. Pinheiro ◽  
Rinaldo A. Mota ◽  
Maria L. R. S. Cunha ◽  
Carlos A. M. Lopes ◽  
...  
Keyword(s):  
Bubalus Bubalis ◽  
Latex Agglutination ◽  
Buffalo Milk ◽  
Coagulase Negative Staphylococcus ◽  
Defibrinated Sheep Blood ◽  
Antimicrobial Sensitivity ◽  
Diffusion Technique ◽  
Amoxicillin Clavulanic Acid ◽  
Toxic Shock Syndrome Toxin ◽  
Staphylococcus Spp

The objective of the current study was to isolate and identify phenotypes of Staphylococcus spp. strains derived from buffalo ( Bubalus bubalis) milk. A total of 548 milk samples from 137 buffalo were cultured in Columbia agar enriched with 5% defibrinated sheep blood. Determination of the capacity of Staphylococcus aureus to produce enterotoxins A–D and toxic shock syndrome toxin-1 (TSST-1) was achieved by reverse passive latex agglutination (RPLA). Antimicrobial sensitivity of S. aureus strains was evaluated using the disk diffusion technique, and β-lactamase detection was achieved using the chromogenic test with paper discs impregnated with nitrocefin. From all the mammary quarters examined, 36 (10.8%) were positive for Staphylococcus spp., 83.3% were coagulase-negative staphylococcus (CNS), 11.1% were coagulase-positive staphylococcus (CPS), and 5.6% were of CPS+CNS positive. All isolates of S. aureus produced at least 1 toxin and 5 out of 6 isolates (83.0%) produced β-lactamase. One hundred percent of S. aureus isolates were sensitive to methicillin and amoxicillin + clavulanic acid, and resistant to ampicillin, penicillin, and oxacillin. Analysis of the results obtained in the current study highlight the epidemiologic importance of buffalo milk regarding the production of enterotoxins and TSST-1 and the potential risk to public health.

Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69

Chemotherapy ◽  
2021 ◽  
pp. 1-7
Author(s):  
Carla Adriana dos Santos ◽  
Rodrigo Tavanelli Hernandes ◽  
Marcos Paulo Vieira Cunha ◽  
Filipe Onishi Nagamori ◽  
Claudia Regina Gonçalves ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Susceptibility Testing ◽  
Polymyxin B ◽  
Antimicrobial Treatment ◽  
Sequence Type ◽  
Broth Microdilution ◽  
Economic Costs ◽  
Novel Mutations ◽  
E Coli ◽  
Total Inhibition

<b><i>Background:</i></b> Uropathogenic <i>Escherichia coli</i> (UPEC) are frequent pathogens worldwide, impacting on the morbidity and economic costs associated with antimicrobial treatment. <b><i>Objectives:</i></b> We report two novel mutations associated with polymyxin-B resistance in an UPEC isolate collected in 2019. <b><i>Methods:</i></b> Isolate was submitted to antimicrobial susceptibility testing including broth microdilution for polymyxin B. Whole genome was sequenced and analyzed. <b><i>Results:</i></b> Polymyxin-B total inhibition occurred at 16 mg/L (resistant). UPEC isolate was assigned to the phylogroup D, serotype O117:H4, and Sequence Type 69. <i>mcr</i> genes were not detected, but two novel mutations in the <i>pmrA/basS</i> (A80S) and <i>pmrB/</i>basR (D149N) genes were identified. <b><i>Conclusions:</i></b> The occurrence of non-<i>mcr</i> polymyxin resistance in <i>E. coli</i> from extraintestinal infections underscores the need of a continuous surveillance of this evolving pathogen.

Declining Susceptibility to Neomycin and Polymyxin B of Pathogens Recovered in Otitis Externa Clinical Trials

2004 ◽  
Vol 97 (5) ◽  
pp. 465-471 ◽  
Author(s):  
H. Furman Cantrell ◽  
Elyane E. Lombardy ◽  
Frederick P. Duncanson ◽  
Ephraim Katz ◽  
Joseph S. Barone
Keyword(s):  
Clinical Trials ◽  
Otitis Externa ◽  
Polymyxin B

scholarly journals Tetracycline Susceptibility Testing and Resistance Genes in Isolates of Acinetobacter baumannii-Acinetobacter calcoaceticus Complex from a U.S. Military Hospital

2009 ◽  
Vol 53 (6) ◽  
pp. 2693-2695 ◽  
Author(s):  
Kevin S. Akers ◽  
Katrin Mende ◽  
Heather C. Yun ◽  
Duane R. Hospenthal ◽  
Miriam L. Beckius ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Resistance Genes ◽  
Susceptibility Testing ◽  
Tetracycline Resistance ◽  
Acinetobacter Calcoaceticus ◽  
Multidrug Resistant ◽  
Military Hospital ◽  
Tetracycline Resistance Genes ◽  
Testing Method

ABSTRACT Infections with multidrug-resistant Acinetobacter baumannii-Acinetobacter calcoaceticus complex bacteria complicate the care of U.S. military personnel and civilians worldwide. One hundred thirty-three isolates from 89 patients at our facility during 2006 and 2007 were tested by disk diffusion, Etest, and broth microdilution for susceptibility to tetracycline, doxycycline, minocycline, and tigecycline. Minocycline was the most active in vitro, with 90% of the isolates tested susceptible. Susceptibilities varied significantly with the testing method. The acquired tetracycline resistance genes tetA, tetB, and tetA(39) were present in the isolates.

scholarly journals Otodectic and bacterial etiology of feline otitis externa in Tripoli, Libya

2021 ◽  
Vol 10 (4) ◽  
pp. 377-383
Author(s):  
Murad A. Hiblu ◽  
Omar M. Ellraiss ◽  
Elfurgani S. Karim ◽  
Rabia A. Elmishri ◽  
Enass M. Duro ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Otitis Externa ◽  
Diffusion Method ◽  
Bacterial Isolates ◽  
Disk Diffusion Method ◽  
Parasite Detection ◽  
Common Etiology ◽  
Etiological Agents ◽  
Staphylococcus Spp ◽  
Otodectes Cynotis

Background: Feline otitis externa is a dermatological disorder with a multifactorial complex etiology.Aim: This study aimed to investigate the prevalence of different etiological agents, particularly the parasitic and bacterial, responsible for the cases of feline otitis externa in Tripoli, Libya, and to assess the antimicrobial susceptibility of the bacterial isolates from those cases.Methods: Cerumen and otic discharges of the suspected cats were collected for parasite detection and bacterial culture. Kirby–Bauer’s disk diffusion method was used for antimicrobial susceptibility testing.Results: The results showed that otodectic mites and bacterial causes were equally the most prevalent in those cases, with a prevalence of 47.1% each. Otodectes cynotis infestation was more frequently bilateral and severe. Staphylococcus spp. were the most prevalent among bacterial causes (75%), followed by Proteus spp. (16.6%) and Pseudomonas spp. (8.4%). Norfloxacin and gentamicin were the most effective antimicrobials against bacterial isolates, as they were effective against 83.3% and 70.8% of isolates, respectively.Conclusion: Otodectes cynotis infestation and staphylococcal infections constituted the most common etiology of feline otitis externa in Tripoli, Libya, and norfloxacin represented a cogent antibacterial for the treatment of otitis externa.

scholarly journals Unusual Occurrence of Salmonella Mikawasima in 2012–2013 in the Czech Republic: Part of a Multistate Outbreak?

2014 ◽  
Vol 63 (3) ◽  
pp. 355-357 ◽  
Author(s):  
PETRA MYŠKOVÁ ◽  
RENÁTA KARPÍŠKOVÁ
Keyword(s):  
Czech Republic ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
European Countries ◽  
Quinolone Resistance ◽  
Environmental Isolates ◽  
The Czech Republic ◽  
Unusual Occurrence ◽  
Microbial Agents

An increased number of cases of salmonellosis caused by the rare serotype Mikawasima was noted in the years 2012 and 2013 in the Czech Republic. A potential on-going outbreak caused by this serotype in European countries has also been reported. Altogether 14 human and 1 environmental isolates from the year 2012 and 11 human isolates from the year 2013 from different locations of the Czech Republic were sent to our laboratory for typing. Macrorestriction analysis together with antimicrobial susceptibility testing and PCR for ESBL and plasmid-mediated quinolone resistance detection were performed to compare our isolates. Twenty-one isolates created two very similar clusters and 5 isolates had different profiles. Twenty-five isolates were fully susceptible to all agents used. One isolate showed resistance to 12 microbial agents and possessed blaTEM, blaSHV, blaOXA, blaCTX-M and qnrB genes.

Efficacy and safety of topical ciprofloxacin/dexamethasone versus neomycin/polymyxin B/ hydrocortisone for otitis externa

2004 ◽  
Vol 20 (8) ◽  
pp. 1175-1183 ◽  
Author(s):  
Peter S. Roland ◽  
Francis D. Pien ◽  
Craig C. Schultz ◽  
Dan C. Henry ◽  
Peter J. Conroy ◽  
...  
Keyword(s):  
Otitis Externa ◽  
Polymyxin B ◽  
Efficacy And Safety

scholarly journals Antimicrobial susceptibility and molecular characterization of Salmonella serovar Ndolo isolated from outbreaks in cattle and horses

2018 ◽  
Vol 48 (12) ◽  
Author(s):  
Carolina Pantuzza Ramos ◽  
Rafael Gariglio Clark Xavier ◽  
Carlos Augusto Gomes Leal ◽  
Elias Jorge Facury Filho ◽  
Antonio Ultimo de Carvalho ◽  
...  
Keyword(s):  
Clavulanic Acid ◽  
Antimicrobial Susceptibility ◽  
Virulence Genes ◽  
Susceptibility Testing ◽  
Domestic Animals ◽  
Amoxicillin Clavulanic Acid ◽  
Resistant Strains ◽  
Human Infections ◽  
First Time

ABSTRACT: The present study aimed to describe and characterize, for the first time, two outbreaks of salmonellosis caused by Salmonella Ndolo in foals and calves in Brazil and compare the isolated strains with S. Ndolo previously identified in asymptomatic reptiles. The affected calves and foals presented fever, lethargy, and profuse diarrhea. Isolated strains were subjected to antimicrobial susceptibility testing, characterized according to virulence genes, and fingerprinted by ERIC-PCR. Salmonella Ndolo was identified in fecal samples from two foals and four calves. One isolate from a calf was resistant to amoxicillin/clavulanic acid, trimethoprim/sulfamethoxazole, and florfenicol. Strains from two other calves were resistant to oxytetracycline. All virulence genes tested were present in the isolates, and two major clusters of closely related strains were identified by ERIC-PCR, each per outbreak. This is the first report of Salmonella Ndolo infection in domestic and symptomatic animals. Previously, this serovar had been identified only in human infections. The presence of relevant virulence genes in all Salmonella Ndolo isolates and the detection of antimicrobial multi-resistant strains highlighted the importance of monitoring serovars associated with salmonellosis in domestic animals.

Sign in / Sign up

Export Citation Format

Share Document