scholarly journals Microbiological and antibacterial resistance profile in canine otitis externa – a comparative analysis

2019 ◽  
Vol 22 (4) ◽  
pp. 447-456
Author(s):  
V. Petrov ◽  
G. Zhelev ◽  
P. Marutsov ◽  
K. Koev ◽  
S. Georgieva ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Otitis Externa ◽  
Bacterial Species ◽  
Antibacterial Resistance ◽  
Clear Trend ◽  
Malassezia Pachydermatis ◽  
Resistance Patterns ◽  
Amoxicillin Clavulanic Acid ◽  
Microbial Agents ◽  
Coagulase Positive Staphylococci

The aim of the present study was to compare the prevalence of microbial agents involved in canine otitis externa and their sensitivity to antibacterial drugs in two periods: 2007–2011 and 2013–2017. For 2013–2017, coagulase-positive staphylococci were the dominating bacterial species (186 isolates), followed by Pseudomonas aeruginosa (82 strains). The rate of isolated yeasts (mainly Malassezia pachydermatis) was substantially high (152 isolates). Compared to the earlier period (2007–2011) a tendency to more frequent occurrence of co-infections was noted – 61.7% and more than 80% of co-infections involved yeasts. Antibacterial resistance patterns showed a clear trend to increased resistance of coagulase-positive staphylococci and β-haemolytic streptococci to amoxicillin/clavulanic acid (42% and 50% respectively) and gentamicin (29%, 40%). Increased resistance of P. aeruginosa was established to gentamicin (16%) and amikacin (18%). The prevalence of pseudomonads resistant to enrofloxacin was lower (27%).

scholarly journals Identification and antimicrobial susceptibility of microbial agents of otitis externa in dogs

2022 ◽  
Vol 74 (10) ◽  
pp. 6136-2022
Author(s):  
SERPIL KAHYA DEMIRBILEK ◽  
ÖZGE YILMAZ
Keyword(s):  
Otitis Externa ◽  
Susceptibility Testing ◽  
Acinetobacter Calcoaceticus ◽  
Trichophyton Mentagrophytes ◽  
Polymyxin B ◽  
Malassezia Pachydermatis ◽  
Antimicrobial Sensitivity ◽  
Amoxicillin Clavulanic Acid ◽  
Staphylococcus Spp ◽  
Microbial Agents

In this study, a total of 277 unmedicated dogs with otitis externa were used. Overall, 413 agents were isolated from 277 ear swab samples; 52.7% of the cases were mono-infections (146 cases), and 37.1% of the cases were poly-infections (103 cases). In 10.1% (28) of the cases, neither bacteria nor yeasts were isolated. Coagulase-positive Staphylococcus spp. were the most frequently isolated bacteria and were found in 90 (21.8%) of the samples. Fifty-eight samples, (14%) were positive for Staphylococcus aureus, 51 (12.3%) for Pseudomonas aeruginosa, 27 (6.5%) for Proteus mirabilis, 27 (6.5%) for Malassezia pachydermatis, 21 (5%) for Corynebacterium spp., 21 (5%) for β-haemolytic Streptococcus spp., 15 (3.6%) for Staphylococcus pseudointermedius, 12 (2.9%) for Proteus spp., 12 (2.9%) for Escherichia coli, 9 (2.1%) for Acinetobacter calcoaceticus, 7 (1.6%) for Trichophyton mentagrophytes, 5 (1.2%) for Staphylococcus auricularis, and 46 (11.1%) for different bacteria and yeasts. A total of 14 different bacteria and yeasts were isolated and identified. Kirby-Bauer antibiotic susceptibility testing was carried out for 10 different antibiotics. The bacterial isolates were found to be resistant to amoxicillin-clavulanic acid (45%), gentamycin (28%), ampicillin/cloxacillin (69%), tobramycin (28%), amikacin (23%), enrofloxacin (47%), chloramphenicol (58%), doxycycline (65%), lincomycin/spectinomycin (58%) and polymyxin B (62%). In conclusion, it is important to test the antimicrobial sensitivity of aetiological agents of otitis externa before treatment so as to prevent the development of antibiotic resistance in bacteria and yeasts.

scholarly journals Frequency and Antimicrobial Resistance Patterns of Bacterial Species Isolated from the Body Surface of the Housefly (Musca domestica) in Akure, Ondo State, Nigeria

2020 ◽  
Author(s):  
Babatunde Odetoyin ◽  
Babatunde Adeola ◽  
Olarinde Olaniran
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacterial Species ◽  
Bacterial Load ◽  
The Body ◽  
Resistant Bacteria ◽  
Salmonella Spp ◽  
Isolation Technique ◽  
Garbage Dump ◽  
Antibiotic Susceptibility Test ◽  
Resistance Patterns

Background: The emergence and spread of antibiotic resistant bacteria has become a serious problem worldwide. House­flies are potential carriers of pathogenic and resistant bacteria and could be contributing to the global spread of these strains in the environments.We investigated the prevalence and antimicrobial resistant profiles of bacteria isolated from houseflies in Akure. Methods: Twenty-five houseflies were captured by a sterile nylon net from the slaughterhouse, garbage dump, human house, hospital, and eatery from 9:00am to 1:00pm when the flies were active and transported immediately to the la­bor­atory in sterile containers for processing. Bacterial loads were enumerated by serial dilution and plating on nutrient agar and selective media. Bacteria species were isolated by conventional isolation technique. Antibiotic susceptibility test was determined by the Kirby-Bauer disc diffusion technique. Results: Sixty-seven bacterial species were isolated from 25 samples that were collected. The predominant bacterial species was Escherichia coli (n= 31, 45%), followed by Klebsiella pneumoniae (n= 17, 25%), Staphylococcus aureus (n= 11, 16%) and Pseudomonas aeruginosa (n= 3, 4.3%). The bacterial load of the samples ranged from 9.7×105CFU/mL to 1.65×106CFU/mL. The results revealed that all isolates of Pseudomonas aeruginosa, Salmonella spp, and Proteus mi­rabilis were resistant to streptomycin and cotrimoxazole, augmentin and amoxicillin respectively. None of the S. aureus iso­lates was resistant to cotrimoxazole, chloramphenicol, sparfloxacin, augmentin, and ofloxacin. All isolates were multi-drug resistant. Conclusion: House flies that were collected from the slaughterhouse, garbage dump, human house, hospital, and eatery may participate in the dispersal of pathogenic and resistant bacteria in the study environment.

scholarly journals Antimicrobial Resistance Patterns in Diabetic Foot Infections, an Epidemiological Study in Northeastern Italy

Antibiotics ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1241
Author(s):  
Giovanni Boschetti ◽  
Dino Sgarabotto ◽  
Marco Meloni ◽  
Marino Bruseghin ◽  
Christine Whisstock ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Diabetic Foot ◽  
Staphylococcus Epidermidis ◽  
Bacterial Species ◽  
Morganella Morganii ◽  
Diabetic Foot Infections ◽  
Resistance Patterns ◽  
Northeastern Italy ◽  
Additional Resistance ◽  
One Year

This study is a retrospective epidemiological assessment of bacterial species isolated from a cohort of out-patients with diabetic foot infections referred to our “Diabetic Foot Unit” over one year, with particular attention to index pathogens, as identified by the EARS Network. Staphylococcus aureus and Pseudomonas aeruginosa accounted for 33.5% and 11.9% of cases, respectively. MRSA was isolated in 27.1% of patients, with 14.06% showing additional resistance to three antimicrobial classes. Pseudomonas aeruginosa presented extensive resistance to fluoroquinolones (57.3%), which was associated with resistance to piperacillin in 17.6% or to carbapenems in 23.5% of cases. Other pathogens, such as methicillin resistant Staphylococcus epidermidis, Escherichia coli and Morganella morganii ESBL and Enterococcus faecium VRE, were also found.

scholarly journals Detection of extended spectrum beta-lactamase genes in Pseudomonas aeruginosa isolated from patients in rural Eastern Cape Province, South Africa

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mojisola C. Hosu ◽  
Sandeep D. Vasaikar ◽  
Grace E. Okuthe ◽  
Teke Apalata
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Resistance ◽  
Multidrug Resistant ◽  
High Rate ◽  
Infection Prevention And Control ◽  
Beta Lactamase ◽  
Eastern Cape ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Resistance Patterns

AbstractThe proliferation of extended spectrum beta-lactamase (ESBL) producing Pseudomonas aeruginosa represent a major public health threat. In this study, we evaluated the antimicrobial resistance patterns of P. aeruginosa strains and characterized the ESBLs and Metallo- β-lactamases (MBL) produced. Strains of P. aeruginosa cultured from patients who attended Nelson Mandela Academic Hospital and other clinics in the four district municipalities of the Eastern Cape between August 2017 and May 2019 were identified; antimicrobial susceptibility testing was carried out against thirteen clinically relevant antibiotics using the BioMérieux VITEK 2 and confirmed by Beckman autoSCAN-4 System. Real-time PCR was done using Roche Light Cycler 2.0 to detect the presence of ESBLs; blaSHV, blaTEM and blaCTX-M genes; and MBLs; blaIMP, blaVIM. Strains of P. aeruginosa demonstrated resistance to wide-ranging clinically relevant antibiotics including piperacillin (64.2%), followed by aztreonam (57.8%), cefepime (51.5%), ceftazidime (51.0%), piperacillin/tazobactam (50.5%), and imipenem (46.6%). A total of 75 (36.8%) multidrug-resistant (MDR) strains were observed of the total pool of isolates. The blaTEM, blaSHV and blaCTX-M was detected in 79.3%, 69.5% and 31.7% isolates (n = 82), respectively. The blaIMP was detected in 1.25% while no blaVIM was detected in any of the strains tested. The study showed a high rate of MDR P. aeruginosa in our setting. The vast majority of these resistant strains carried blaTEM and blaSHV genes. Continuous monitoring of antimicrobial resistance and strict compliance towards infection prevention and control practices are the best defence against spread of MDR P. aeruginosa.

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