scholarly journals Extracts of Rhodiola rosea L. and Scutellaria galericulata L. in functional dairy products

2020 ◽  
pp. 163-170 ◽  
Author(s):  
Alexandra Zaushintsena ◽  
Evgeny Bruhachev ◽  
Olga Belashova ◽  
Lyudmila Asyakina ◽  
Marina Kurbanova ◽  
...  
Keyword(s):  
Dairy Products ◽  
Dairy Product ◽  
Daily Intake ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Rhodiola Rosea ◽  
Cottage Cheese ◽  
Mountain Areas ◽  
Rock Outcrops ◽  
Active Substances

Introduction. Modern scientific research into the biochemical composition and medicinal value of plants makes it possible to use them as functional ingredients in food technology. The research objective was to test rose root (Rhodiola rosea L.) and scullcap (Scutellaria galericulata L.) for biologically active substances and their potential use in functional dairy products. Study objects and methods. The research featured biologically active substances (BAS) obtained from rose root and scullcap that grow in mountain areas or on rock outcrops along Siberian rivers. The BAS content was determined using high performance liquid chromatography (HPLC). The biologically active substances were screened and identified using HPLC, thin-layer chromatography (TLC), and infra-red identification (IR). The new functional products were based on whey and cottage cheese made from processed whole milk. Results and discussion. The analysis of Rhodiola rosea rhizomes and roots showed the following BAS content (mg/g): rosavin – 16.9, salidroside – 14.3, rosin – 5.04, rosarin – 2.01, and methyl gallate – 6.8. The roots of Scutellaria galericulata had the following BAS content (mg/g): scutellarein – 22.27, baicalin – 34.37, baicalein – 16.30, apigenin – 18.80, chrysin – 6.50, luteolin – 5.40, and vogonin – 3.60. Whey served as a basis for a new functional whey drink fortified with BAS isolated from Rhodiola rosea 100 mL of the drink included 50 mL of whey, 20 mL of apple juice, 0.1 mL of rose root concentrate, 3 g of sugar, 0.5 g of apple pectin, 04 g of citric acid, and 30 mL of ionized water. The content of phytochemical elements ranged from 0.11 ± 0.001 to 0.49 ± 0.08 mg/100 g. Cottage cheese served as a basis for another dairy product fortified with BAS obtained from Scutellaria galericulata. The formulation included 81 g of cottage cheese, 10 mL of cherry jam, 9 g of sugar, and 0.025 mL of scullcap concentrate. The content of biologically active substances in the finished product varied from 0.09 ± 0.02 for luteolin to 0.48 ± 0.11 for baicalin. The whey drink fortified with the BAS extracted from Rhodiola rosea and the cottage cheese product fortified with the BAS isolated from Scutellaria galericulata satisfied 40–45% and 55–60% of the reference daily intake for phenolic compounds, respectively. The obtained data made it possible to recommend the new functional foods for commercial production. Conclusion. A set of experiments was performed to isolate biologically active substances from Rhodiola rosea and Scutellaria galericulata. The research developed and tested formulations of two new functional products based on whey and cottage cheese.

scholarly journals Biologically Active Substances of Siberian Medical Plants in Functional Wgey-Based Drinks

2019 ◽  
Vol 49 (1) ◽  
pp. 14-22
Author(s):  
Светлана Иванова ◽  
Svetlana Ivanova ◽  
Ирина Милентьева ◽  
Irina Milenteva ◽  
Людмила Асякина ◽  
...  
Keyword(s):  
Callus Cultures ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Rhodiola Rosea ◽  
Plant Materials ◽  
Rhaponticum Carthamoides ◽  
Wide Range ◽  
Mountain Ash ◽  
Active Substances ◽  
Mineral Base

The use of infusions and extracts from domestic plant materials is a promising direction in the development of functional beverages since they contain a wide range of substances of various pharmacological properties. Drinks fortified with physiologically active natural components maintain a certain level of this content in human body. They can have a healing or prophylactic effect. However, there is a lack of technologies for the effective production of biologically active substances from plant materials. Moreover, the development of cultivated botanical medicinal plant species remains quite poor. The present study features the qualitative and quantitative composition of biologically active substances of medicinal plants that are endemics of Siberia. They are Rhodiola rosea (Rhodiola rosea L.) and maral root (Rhaponticum carthamoides). The paper introduces a method for increasing their biosynthetic activity. An experiment helped to select a composition of the nutrient medium for the cultivation of callus cultures of Rhodiola rosea (Rhodiola rosea L.) and maral root (Rhaponticum carthamoides) in vitro, which contributed to an increase in the biosynthesis of biologically active substances. For callus cultures of Rhodiola Rosea (line R.r-1k) the following composition was used: mineral base – MS; sucrose – 30 g; inositol – 100 mg; thiamine – 1.0 mg; pyridoxine – 1.0 mg; Ca-panthetonate – 10 mg; kinetin – 0.05 g; naphthyl acetic acid – 0.1 g; 2.4-D – 0.5. For callus cultures of maral root (line R.c -2k): mineral base – SH; sucrose – 30 g; inositol – 100 mg; thiamine – 5.0 mg; pyridoxine – 0.5 mg; nicotinic acid – 5.0 mg; kinetin – 0.1 g; indoleacetic acid – 1.0 g. The authors developed a technology for the production of functional whey-based tonic drink fortified with extract of carotenoids isolated from the fruits of mountain ash and the extract of biologically active substances Rhodiola rosea and maral root. The presence of biologically active substances in the plant and medicinal raw materials gives the drink antioxidant and bactericidal properties, as well as helps to raise the overall state of the organizm and strengthen the immune system.

scholarly journals Photosynthetic pigments and phenolic potential of Rhodiola rosea L. from plant communities of different ecology and geography

2020 ◽  
Vol 50 (3) ◽  
pp. 393-403
Author(s):  
Irina Sergeeva ◽  
Alexandra Zaushintsena ◽  
Evgeniy Bryukhachev
Keyword(s):  
Gallic Acid ◽  
Plant Communities ◽  
Photosynthetic Pigments ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Rhodiola Rosea ◽  
Methyl Gallate ◽  
Gorny Altai ◽  
Maximum Content ◽  
Active Substances

Introduction. Longitudinal studies of human activity and metabolism revealed various anti-inflammatory, immunomodulatory, antistress, antioxidant, and adaptogenic properties of such secondary plant metabolites as phenolic compounds and pigments. Human cells cannot synthesize these compounds. Therefore, food biotechnology requires new data on the photosynthetic potential of plants with good functional prospects. The research objective was to study the qualitative and quantitative profile of biologically active compounds of Rhodiola rosea L. harvested from various plant communities in order to define the potential of their extracts and minor compounds for food technologies. Study objects and methods. The research featured three communities of Rhodiola rosea L. originally located in ecologically and geographically different habitats. They were introduced into Kuzbass from the Kuznetsk Alatau, Gorny Altai, and the Tunka alpine tundra belt in Buryatia. The experiment began in 2018, when the rhizomes were dissected into equal shares of 40–42 g and placed in a medicinal plant nursery. The methods of high-performance liquid (HPLC) and thin-layer (TLC) chromatography were used to study the biologically active substances in the plant biomass. The photosynthetic pigments were detected using the spectrophotometric method. The obtained data underwent a statistical analysis based on Statistica 6.0 software. Results and discussion. The sample from the Gorny Altai community revealed twelve biologically active substances. Its rhizomes appeared rich in gallic acid with the maximum content (mg/g) of 10.26 ± 2.31, rosein (20.45 ± 3.46), daphneticin (13.80 ± 2.30), and salidroside (28.16 ± 2.27). The tops demonstrated the maximum content (mg/g) of astragaline (38.94 ± 2.21), tricine (13.07 ± 0.72), tricine-5-O-β-D-glucopyranoside (35.25 ± 1.66), tricine-7-O-β-D-glucopyranoside (30.23 ± 1.45), and tyrosol (21.80 ± 1.21). The Kuznetsk Alatau sample proved to possess five biologically active substances. Its rhizomes had the maximum content (mg/g) of rosavin (16.89 ± 2.11) and salidroside (14.35 ± 2.52). The sample obtained from the Tunka ridge in Buryatia had six biologically active substances with the maximum content (mg/g) of rosavin (20.72 ± 2.11), methylgalate (39.00 ± 1.05), and cinnamaldehyde (10.15 ± 1.93) in the rhizomes. The top biomass of Rhodiola rosea L. accumulated about 0.333 mg/g of chlorophylls and synthesized 0.109 mg/g of carotenoids on average. The research established the correlation coefficients between the content of photosynthetic components with morphometric characteristics, including two positive dependences between the content of carotenoids and the number of leaves (r = 0.89 ± 0.09) and the content of carotenoids and shoot length (r = 0.96 ± 0.22). Conclusion. The samples of Rhodiola rosea L. demonstrated a good biotechnological potential for medicine and food industry. The Kuznetsk Alatau plant community proved rich in rosavin, salidroside, and methyl gallate. The Gorny Altai samples revealed high content of salidroside, gallic acid, daphnetitsin, and rosein. The Tunka samples appeared to synthesize a lot of methyl gallate, rosavin, and cinnamaldehyde. In addition, the top biomass of the Altai sample proved rich in tricine and its derivatives, astragaline and tyrosol. The research also established the possibility of commercial extraction of photosynthetic pigments from the top biomass of Rhodiola rosea L. for functional food production.

scholarly journals Opportunities for using biologically active substances Rhodiola rosea L. in the production of functional food with consideration for antimicrobial activity

2020 ◽  
Vol 176 ◽  
pp. 01011
Author(s):  
Alexandra Zaushintsena ◽  
Olga Babich ◽  
Evgeny Bryukhachev ◽  
Irina Milenteva ◽  
Anton Starcev
Keyword(s):  
Antimicrobial Activity ◽  
Gallic Acid ◽  
High Performance ◽  
Growth Zone ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Rhodiola Rosea ◽  
Diffusion Method ◽  
Antibiotic Effect ◽  
Active Substances

Biologically active substances extricated by extraction of water and 40% ethyl alcohol from rootstalks of Rhodiola rosea L.: gallic acid, forms of rosovin and salidroside have been studied. Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) were used during the research. Determination of antimicrobial activity was performed by agar diffusion method (OFC.1.2.4.0010.15) [8]. The method is based on the evaluation of inhibition of the growth zone of testing cultures of microorganisms by certain concentrations of biologically active substances (BAS). String formed bacteria (8 strains), cocci bacteria (3) and fungal (4) microorganisms, bifidobacterium (10 strains) were used as testing cultures. Various responses of microorganisms to exposure by biologically active substances have been determined. Gallic acid and salidroside in the aqueous phase have the maximum inhibitory property to rod bacteria and coccoid microorganisms. Post-antibiotic effect is shown in rozovin. Salidroside in the butanol phase is more neutral to most microorganisms, with the exclusion of: Acinetobacter baumannii, Pseudomonas aeruginosa, Bacillus cereus and Staphylococcus aureus. The lack of a clearly expressed reaction of BAS to bifidobacterium, most probably, gives the possibility of their use in the development of functional products of a new generation. However, first of all, it is required to organize a special experiment on the structure of the introduction of the studied components into the lactoserum. Next, determine the content of BAS in the derived product, the reaction of bifidobacterium admitted for use in biotechnology, and a standard set of microbiological strains. Gallic acid is suggested to be introduced in applied studies to explore as a target immunomodulator of resistance to the listed microflora and to develop an effective form of a new product.

scholarly journals Determination of the Qualitative Composition of Biologically-Active Substances of Extracts of In Vitro Callus, Cell Suspension, and Root Cultures of the Medicinal Plant Rhodiola rosea

Biomolecules ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 365 ◽  
Author(s):  
Lyudmila Asyakina ◽  
Stanislav Sukhikh ◽  
Svetlana Ivanova ◽  
Alexander Prosekov ◽  
Elena Ulrikh ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Cell Suspension ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Rhodiola Rosea ◽  
Root Cultures ◽  
Qualitative Composition ◽  
Callus Cell ◽  
Active Substances

The results of the qualitative composition analysis of the dried biomass extracts of in vitro callus, cell suspension, and root cultures show that the main biologically active substances (BAS) in the medicinal plant, Rhodiola rosea, are 6-C-(1-(4-hydroxyphenyl)ethyl)aromadendrin (25 mg, yield 0.21%), 2-(3,7-dihydroxy-2-(2-hydroxypropan-2-yl)-2,3-dihydrobenzofuran-5-yl)-6,7-dihydroxychroman-4-one (23 mg, yield 0.2%), 2-(3,4-dimethoxyphenyl)-5,7-dimethoxychroman-4-one (175 mg, yield 1.5%), 5,7-dihydroxy-2-(4-hydroxy-3-(2-(4-hydroxyphenyl)-4-oxo-4H-chromen-6-yl)phenyl)-4H-chromen-4-one (45 mg, yield 0.5%), 5,6,7,8-tetrahydroxy-4-methoxyflavone (0.35 mg, 0.5%). BAS from the dried biomass extracts of in vitro callus, cell suspension, and root cultures of Rhodiola rosea will be used for the production of pharmaceuticals and dietary supplements with antitumor, antimicrobial, and antioxidant effects.

scholarly journals Development of technology for producing essential fatty acids from hydrolysates of high-fat fish

2021 ◽  
Vol 23 (2) ◽  
pp. 119-130
Author(s):  
Elena E. Kuprina ◽  
Evgeniya S. Grishina ◽  
Anastasia N. Yakkola ◽  
Andrey N. Manuilov ◽  
Pavel I. Demidov ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fish Oil ◽  
Electrochemical Method ◽  
Essential Fatty Acids ◽  
Daily Intake ◽  
Food Products ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Omega 3 ◽  
Active Substances

The technology for obtaining biologically active substances of a lipoid nature, enriched with omega-3 acids, from waste from the processing of hydrobionts by means of electrochemical hydrolysis and cryoconcentration has been developed. A comparative analysis of the composition of wastes from cutting herring and trout is carried out, and the expediency of their use for obtaining biologically active substances of a lipoid nature is shown. A technological scheme has been developed and fat yields have been determined when it is obtained from fish waste by an electrochemical method. The fatty acid composition of the fat obtained by the electrochemical method has been determined. It was found that cryoconcentrated fat obtained from wastes from trout and herring cutting by the electrochemical method has a significantly increased content of omega-3 acids and, accordingly, biological value compared to edible and medical fish oil from the liver of the cod family. It was found that during cryoconcentration, the concentration of polyunsaturated fatty acids increases, reaching values close to 90%, which allows the resulting product to be classified as biologically active additives. It was shown by calculation that to create functional fish-based food products from fish of the salmon family, it is sufficient to introduce 4 g of the obtained biologically active additive per 100 g of the product. There is also an improvement in the organoleptic properties of foods from lean fish species. It has been shown that in order to meet 30% of the recommended daily intake of omega-3 acids in the development of functional food products based on rainbow trout and Atlantic herring, it is necessary to introduce 1.98 g and 1.8 g of cryoconcentrated fish oil. After encapsulation in nanocapsules, the drug will be suitable for enrichment with omega-3 acids in any food products, which is the subject of further research.

BIOTECHNOLOGICAL PROCESS OF CREATING FUNCTIONAL DAIRY PRODUCT ENRICHED WITH THE EXTRACT OF SCUTELLARIA GALERICULATA L.

2020 ◽  
Author(s):  
O.V. Belashova ◽  
◽  
A.V. Zaushintsena ◽  
N.V. Fotina
Keyword(s):  
Dairy Product ◽  
The Body ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Therapeutic Effects ◽  
Scutellaria Baicalensis Georgi ◽  
Physico Chemical ◽  
Functional Product ◽  
Wide Range ◽  
Active Substances

One of the ways to eliminate population diseases and increase the body is resistance to adverse environmental factors is the systematic use of functional food products enriched with a complex of biologically active substances with a wide range of therapeutic effects. The creation of a new, unique functional dairy product was preceded by long-term comparative biochemical studies of Scutellaria baicalensis Georgi and Scutellaria galericulata L. In the grass and roots, biologically active substances of a phenolic nature, such as baicalin, vogonin, scutellarin, etc., were found useful for medicinal purposes. The use of extracts from the of Scutellaria galericulata L. in the development of a functional product based on sour-milk fractions made it possible to obtain curd mass enriched with the biologically active substance of this plant. The functional product meets the organoleptic, physico-chemical, tasting requirements for new functional products and is recommended for introduction into the diet of different population groups by age and functional activity.

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