scholarly journals Detection of enteric bacteria in two groundwater sources and associated microbial health risks

2021 ◽  
Vol 19 (2) ◽  
pp. 322-335
Author(s):  
Adewale O. Olalemi ◽  
Oluwasegun M. Ige ◽  
Grace A. James ◽  
Favour I. Obasoro ◽  
Favour O. Okoko ◽  
...  
Keyword(s):  
Water Samples ◽  
Membrane Filter ◽  
Negative Relationship ◽  
Enteric Bacteria ◽  
Gastrointestinal Infections ◽  
E Coli ◽  
Microbiological Methods ◽  
Potential Risks ◽  
Pump Pressure

Abstract The microbial quality of two groundwater sources (well and borehole) and associated risks were quantitatively assessed. Water samples from the selected borehole and well were collected over a period of 12 weeks (n = 48). The concentrations of Escherichia coli, faecal coliforms, Salmonella, Shigella, Clostridium, Bifidobacterium and Campylobacter were determined using standard microbiological methods, which involve the use of a membrane filter technique. The water samples were filtered through a 0.45 μm membrane filter using vacuum pump pressure and plated on selective agar for the bacteria under test. The number of colonies of the bacterial growth observed after the incubation period was counted and recorded. The physicochemical properties of the water were determined using standard methods. The risk of Salmonella, Shigella, Clostridium and Campylobacter infections resulting from the ingestion of water from the borehole and well was estimated. The results showed that the levels of enteric bacteria in the borehole were higher than those in the well. The mean levels of E. coli in water from the borehole and well were 3.3 and 1.7 log10 cfu/100 ml, respectively, and exhibited a negative relationship with salinity (r = −0.53). The estimated risks of infection associated with the pathogens in water from the borehole and well were greater than the acceptable risk limit of 10−4 and followed this order Clostridium<Salmonella<Campylobacter<Shigella. The findings of this study suggest recent and continuous faecal contamination of the two groundwater sources, thus exposing the residents relying on the water for drinking to potential risks of gastrointestinal infections.

Microbiological Status and Food Safety Compliance of Commercial Basil Production Systems

2016 ◽  
Vol 79 (1) ◽  
pp. 43-50 ◽  
Author(s):  
WILLEKE de BRUIN ◽  
DENISE OTTO ◽  
LISE KORSTEN
Keyword(s):  
Food Safety ◽  
Salmonella Typhimurium ◽  
Water Samples ◽  
Large Scale ◽  
Safety Management ◽  
Membrane Filter ◽  
Microbiological Quality ◽  
E Coli ◽  
Study Results

ABSTRACT Basil has been implicated in a number of microbe-associated foodborne illnesses across the world, and the source of contamination has often been traced back to the production and/or processing stages of the supply chain. The aim of this study was to evaluate the microbiological quality of fresh basil from the point of production to the retail outlet in the Gauteng and Northwest Provinces of South Africa. A total of 463 samples were collected over a 3-month period from two large-scale commercial herb producing and processing companies and three retail outlets. The microbiological quality of the samples was assessed based on the presence or absence of Escherichia coli O157:H7 and Salmonella Typhimurium and the levels of the indicator bacteria E. coli and total coliforms. Salmonella Typhimurium was detected on four basil samples (0.9%) arriving at the processing facility and at dispatch, but no E. coli O157:H7 was detected throughout the study. Total coliform counts were 0.4 to 4.1 CFU/g for basil, 1.9 to 3.4 log CFU/ml for water, and 0.2 to 1.7 log CFU/cm2 for contact surfaces, whereas E. coli was detected in the water samples and only once on basil. The Colilert-18 and membrane filter methods were used to analyze water samples, and a comparison of results revealed that the Colilert-18 method was more sensitive. Strong evidence suggests that high numbers of coliforms do not necessarily indicate the presence of Salmonella Typhimurium. The study results highlight the importance of effective implementation of food safety management systems in the fresh produce industry.

scholarly journals Analisis Kualitas Fisik dan Bakteriologi (E-Coli) Air Sumur Gali Di Jorong Koto Kaciak Kanagrian Magek Kecamatan Magek

2021 ◽  
Vol 16 (2) ◽  
pp. 69-78
Author(s):  
Syukra Alhamda ◽  
Mila Sari ◽  
Nova Herawati
Keyword(s):  
Water Samples ◽  
Health Workers ◽  
Univariate Analysis ◽  
Well Water ◽  
Gastrointestinal Infections ◽  
Physical Quality ◽  
E Coli ◽  
Dug Wells ◽  
Dug Well

Jorong Koto Kaciak is a fairly high incidence of diarrhea contributed 36% of the incidence of diarrhea in Magek District. This study aims to analyze the Physical Quality and Bacteriology (E-Coli) of Dug Well Water in Jorong Koto Kaciak. This is a descriptive describing the physical quality and bacteriology of community dug well water. The populations were all community dug wells amount 153 units. By using cluster sampling, the sample was determined as much as 5 dug wells. Data collection used observation sheets and data analysis included univariate analysis which was carried out computerized. The results showed that 80% dug well water samples met the physical parameter requirements based on temperature, overall 100% community dug well water met the turbidity requirements <25 NTU / 100 ml, 60% dug well water samples met the physical requirements. odorless and tasteless and overall 100% dug well water samples do not meet the quality requirements of microbiology (e-coli)> 50 MPN / 100 ml It can be concluded that the community dug well water physically meets the quality standard requirements drinking water, but microbiologically it does not meet the health standard requirements, especially the quality of bacteriology, which can increase the risk of gastrointestinal infections for the community (diarrhea). For this reason, it is recommended that the community and health workers in Jorong Koto Kaciak pay more attention to the quality of dug well water.

scholarly journals Microbial quality of beef and hygiene practices in small and medium slaughterhouses and butcheries in Uganda

2020 ◽  
Author(s):  
Juliet Kyayesimira ◽  
Wangalwa Rapheal ◽  
Grace Kagoro Rugunda ◽  
Lejju Julius Bunny ◽  
Morgan Andama ◽  
...  
Keyword(s):  
Microbial Contamination ◽  
Microbiological Quality ◽  
Microbial Load ◽  
Microbial Quality ◽  
E Coli ◽  
Hygiene Practices ◽  
Safe Level ◽  
Microbiological Methods ◽  
Beef Carcass

Abstract Background If hygiene practices along the beef processing nodes at small and medium enterprise (SME) slaughter houses and butcheries are not observed, they may pose a health risk due to microbial contamination. In SME slaughterhouses and butcheries, the risk may be higher due to transmission of foodborne pathogens. This study determined the hygienic practices and microbial quality risk among meat handlers (MH) in SME slaughterhouses and butcheries. Methods Assessment of microbiological quality of beef was carried out at slaughter houses and butcher shops in the districts of Western, Central and Eastern regions of Uganda. A cross sectional study was conducted from June 2017 to January 2018 using observation checklists to record unhygienic practices among the various actors. Microbial load at slaughter and butchery was determined from a total of 317 swab samples collected from carcass, tools, protective clothing and hands of meat handlers. The microbiological quality of beef was evaluated using standard microbiological methods. The samples were inoculated into differential and selective media. Results Butcheries had the highest microbial load on beef carcass ranging from 4.76 log 10 cfu/cm 2 to 7.90 log 10 cfu/cm 2 Total Viable Counts (TVC) while Total Coliform Counts (TCC) ranged from 1.42 log 10 cfu/cm 2 to 3.05 log 10 cfu/cm 2 , E. coli ranged from 0.68 log 10 cfu/cm 2 to 1.06 log 10 cfu/cm 2 and Staphylococcus aureus ranged from 3.25 log 10 cfu/cm 2 to 4.84 log 10 cfu/cm 2 . Salmonella was absent in all the samples analysed. Results of overall microbial quality of beef in Uganda indicated that only TCC (1.60±0.26 log 10 cfu/cm 2 ) of the beef carcass samples at slaughter houses was not significantly above the safe level (p = 0.693). Overall microbial load (TVC, TCC, E. coli and S. aureus ) at butcheries were significantly (p < 0.05) above the safe level. Butcheries of Mbale district had the highest percentage (70%) of beef carcass samples above the TCC safe levels whereas butcheries of Mbarara district had the highest percentage (40%) of beef carcass samples above the E. coli safe levels. TVC from hands and clothes at butchery across the three study districts varied significantly (p=0.007) with the highest counts (7.23 log 10 cfu/cm 2 ) recorded from personnel clothes and lowest (5.46 log 10 cfu/cm 2 ) recorded from hands. On the other hand, swab samples picked from chopping board and working table at the butchery did not show significant variation in TVC, TCC, E. coli and S. aureus microbial loads across the three study districts. Conclusion Hygienic handling of carcasses after slaughter is critical in preventing contamination and ensuring meat safety in informal meat trading sectors in Uganda. Handling practices of beef at Ugandan slaughterhouses and butcheries are not hygienic hence not up to standard and they contribute to microbial contamination of beef posing a risk to consumers. The distribution stage is the most critical period, during which the quality of meat can easily be compromised.

scholarly journals Isolation and Enumeration of Escherichia coli from Soil and Water

2020 ◽  
Vol 36 (2) ◽  
pp. 75-77
Author(s):  
Anindita Bhowmik ◽  
Sunjukta Ahsan
Keyword(s):  
Escherichia Coli ◽  
Water Samples ◽  
Gas Production ◽  
Soil Samples ◽  
Most Probable Number ◽  
Biochemical Tests ◽  
Probable Number ◽  
E Coli ◽  
Dilution Series

Majority of the population of Bangladesh depend on tap or surface water as their source of water supply. This study was carried out to examine the microbial quality of both water and soil collected from different places using the multiple tube fermentation technique to determine coliform count by the most probable number (MPN) method in brilliant green lactose broth (BGLB) media.Inoculum from positive tubes of the presumptive test were further transferred on eosinemethylene blue (EMB) and MacConkey agar.The organisms isolated were further characterized using biochemical tests. Out of 93 water samples, 30 (32.26%) indicated the presence of lactose fermenter and gas producer in all 3 tubes of dilution series using inoculum quantities of 1.0, 0.1 and 0.01 ml, whereas out of 85 soil samples, 45 (52.94%) showed acid and gas production in all 3 tubes of dilution series.Among 85 soil samples, 40 samples that contained at least one positive in each dilution series and among 93 water samples, 31 samples that contained at least one positive in each dilution series were further re-identified with biochemical tests.This study showed 30.59% soil isolates and 26.88% water isolates were Escherichia coli which highlighted the fact that both water and soil act as a major reservoir of E.coli, which indicates possible fecal contamination as well as presence of potentially pathogenic E. coli. Bangladesh J Microbiol, Volume 36 Number 2 December 2019, pp 75-77

COMPARISON OF ESCHERICHIA COLI AND STREPTOCOCCUS FAECALIS AS A TEST ORGANISM TO DETERMINE THE SANITARY QUALITY OF FOOD1 PART I

1954 ◽  
Vol 17 (7) ◽  
pp. 204-218 ◽  
Author(s):  
C. H. Allen ◽  
F. W. Fabian
Keyword(s):  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Intestinal Tract ◽  
Bacterial Contamination ◽  
Test Organism ◽  
Enteric Bacteria ◽  
Streptococcus Faecalis ◽  
E Coli ◽  
Suitable Organism

Since the discovery of the role that bacteria play in disease, a search has been made for a suitable organism that would be indicative of dangerous bacterial contamination in food. Early work with pathogenic bacteria indicated that they would not be suitoble to serve this purpose since they were hard to isolate, were too fastidious, and died off quickly under conditions favorable for keeping food. For these and other reasons the search turned to other bacteria associated with pathogenic bacteria but which were hardier and easier to work with and identify. Fullfilling these requirements were two organisms, E. coli and Strept. faecalis, normal inhabitants of the intestinal tract and associated with many pathogenic bacteria especiallv the enteric bacteria which ore responsible for many food outbreaks. Today the presence of moderate numbers of these organisms is not considered a sure sign of dangerous contamination but rather of possible contamination. Their presence indicates that a search for the cause of the contamination should be made.

scholarly journals Assessment of Drinking Water Quality of Madhyapur-Thimi and Study of Antibiotic Sensitivity against Bacterial Isolates

1970 ◽  
Vol 10 ◽  
pp. 167-172 ◽  
Author(s):  
Bina Laxmi Jayana ◽  
Tista Prasai ◽  
Anjana Singh ◽  
Kayo Devi Yami
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Water Samples ◽  
Enteric Bacteria ◽  
Drinking Water Quality ◽  
National Standard ◽  
Guideline Value ◽  
Guideline Values ◽  
Who Guideline

The study was undertaken to assess the existing status of drinking water quality of Madhyapur-Thimi. A total of 105 water samples comprising 50 (47.61%) wells, 45 (42.82%) tap water and 10 (9.52%) stone spouts were randomly collected. The physicochemical and microbiological parameters were analyzed following standard technique. The pH (1.9%), conductivity (34.28%) and turbidity (16.19%) of water samples had crossed the permissible guideline values as prescribed by WHO and national standard. All samples contained nitrate values within the WHO permissible value as well as national standard but hardness (2%), chloride (2.85%), iron (26.66%), ammonia (11.42%), and arsenic content (1.90%) crossed the WHO guideline value but none of the water samples crossed the national standard for arsenic. Arsenic having 0.025mg/l (25ppb) was detected from two well water samples, Makanani well and Ajimanani well. Total coliform count showed 64.76% of samples crossed the WHO guideline values. Eleven different kinds of enteric bacteria were isolated from different sources, Enterobacter spp. being the predominant. Isolates were subjected for antibiotic susceptibility pattern using ten antibiotics. Among total isolates, resistance was directed to 79.5% Erythromycin, 62.67% Penicillin G, 61.9% Amoxycillin, 34.5% Ampicillin, 21.1% Tetracycline, 15.4% Ceftriaxone, 14.7% Amikacin, 14.7% Cephotaxine, 5.6% Chlorophenical and 5.6% Ofloxacin respectively.Key words: Coliform; Enteric bacteria; Permissible value; Physicochemical; Antibiotic resistanceDOI: 10.3126/njst.v10i0.2955Nepal Journal of Science and Technology Vol. 10, 2009 Page: 167-172

Assessment of microbiological quality of drinking water from household tanks in Bermuda

2008 ◽  
Vol 54 (6) ◽  
pp. 495-500 ◽  
Author(s):  
B. Lévesque ◽  
D. Pereg ◽  
E. Watkinson ◽  
J.S. Maguire ◽  
L. Bissonnette ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Membrane Filtration ◽  
Microbiological Quality ◽  
General Information ◽  
Total Coliforms ◽  
E Coli ◽  
Electoral Register ◽  
Highly Correlated

Bermuda residents collect rainwater from rooftops to fulfil their freshwater needs. The objective of this study was to assess the microbiological quality of drinking water in household tanks throughout Bermuda. The tanks surveyed were selected randomly from the electoral register. Governmental officers visited the selected household (n = 102) to collect water samples and administer a short questionnaire about the tank characteristics, the residents’ habits in terms of water use, and general information on the water collecting system and its maintenance. At the same time, water samples were collected for analysis and total coliforms and Escherichia coli were determined by 2 methods (membrane filtration and culture on chromogenic media, Colilert kit). Results from the 2 methods were highly correlated and showed that approximately 90% of the samples analysed were contaminated with total coliforms in concentrations exceeding 10 CFU/100 mL, and approximately 66% of samples showed contamination with E. coli. Tank cleaning in the year prior to sampling seems to protect against water contamination. If rainwater collection from roofs is the most efficient mean for providing freshwater to Bermudians, it must not be considered a source of high quality drinking water because of the high levels of microbial contamination.

scholarly journals Microbiological quality evaluation of ready-to-eat mixed vegetable salad, food ingredients and some water samples from a restaurant in Accra: A case study

2020 ◽  
Vol 20 (06) ◽  
pp. 16669-16688
Author(s):  
Nii Korley Kortei ◽  
◽  
T Annan ◽  
L Quansah ◽  
G Aboagye ◽  
...  
Keyword(s):  
Public Health ◽  
Water Samples ◽  
Microbial Contamination ◽  
Microbiological Quality ◽  
Food Microbiology ◽  
Plate Count ◽  
Food Ingredients ◽  
Economic Implications ◽  
Microbiological Methods

One serious threat to public health in both developed and developing countries is the microbial contamination of food. This problem poses a great challenge and consequently has economic implications. Causes of microbial contamination are diverse and these may be natural, environmental, or technological. The microbiological quality of most ready-to-eat foods is of great significance to human health because they require minimal or no processing when consumed.The aim of this research was to investigate the microbiological quality of some ready-to-eat mixed vegetable salad foods, ingredients as well as the wash water samples of an urban restaurant located in Accra, Ghana. A total of thirty (30) samples categorized into mixed vegetable salads, foods and water obtained from an urban restaurant in the national capital of Ghana, Accra. They were analyzed at the microbiology laboratory and food microbiology laboratories of School of Allied Health Sciences(UHAS)and Council for Scientific and Industrial Research-Food Research Institute (CSIR-FRI), Ghana,respectively. Standard microbiological methods that are per International Organization for Standardization(ISO) Methods and Nordic Committee on Food Analysis Methods (NMKL) were used in determining the presence and levels of bacteria and fungi.Data obtained were transformed from standard to logarithmic forms and reported as mean+standard deviations. The aerobic plate count samples ranged from 0-4.73 log 10CFU/g. E-coli counts also ranged between 0-2.53 log 10CFU, while Bacillus cereus counts were very low at0-<10log 10CFU/g. Clostridium perfringens and Staphylococcus aureus counts were also very low ranging from0-1.0log10CFU/g.Enterobacteriaceae counts also ranged from 0-1.90 log10CFU/g. Molds and yeasts counts were generally low and ranged from 0-2.48 log 10CFU/g and 0-1.0 log10CFU/g,respectively. None of the samples tested contained Listeria monocytogenes and Salmonella spp. Fungal microbial loads were minimal given the quantities, and were deleterious to the health of consumers. The study revealed that the bacterial loads on mixed vegetable salads, ingredients and water samples used and served by an urban restaurant in Accra were within safe limits according to American Public Health Association (APHA) and International Commission for Microbiological Specifications for Foods (ICMSF) guidelines and,therefore,good for human consumption.

scholarly journals THE EFFECTS OF INDUSTRIAL EFFLUENTS ON THE WATER QUALITY OF ABA RIVER IN SOUTH-EASTERN NIGERIA

2021 ◽  
pp. 121-129
Author(s):  
UBA Uchechukwu Promise ◽  
Dr. Ezekwe Clinton
Keyword(s):  
Water Quality ◽  
Water Samples ◽  
Analytical Method ◽  
Industrial Effluents ◽  
Dry Season ◽  
E Coli ◽  
Water Ph ◽  
Standard Analytical Method ◽  
Temperature Water

This study examines the effect of industrial effluents on the water quality of the Aba River. In order to achieve this, a total of six water samples were collected each season and was analysed for different wastewater characteristics of temperature, water pH, Conductivity, Alkaline, Chloride, BOD5, COD, DO, TS, THC, TDS, Zn, Cd, Fe, Pb, Cr, Total Coli-form and E-Coli. Using APHA Standard Analytical Method, the results shows that during dry season, temperature, pH, Chloride and Zn values were found to be lower than NESREA Standard. While TS, THC and Total Coli-form values were found to be higher than NESREA standard in dry season

scholarly journals Ascertaining the Bacteriological Quality of Water Drawn from Cast and Non-cast Wells in Zaria, Nigeria

2020 ◽  
pp. 34-43
Author(s):  
Danjuma, Florence Yachim ◽  
Onaji, Ajegba Innocent ◽  
Ocheme Julius Okojokwu ◽  
Danladi Yakubu Papi
Keyword(s):  
Public Health ◽  
Drinking Water ◽  
Water Samples ◽  
Dry Season ◽  
Safe Drinking Water ◽  
Internal Wall ◽  
Bacterial Counts ◽  
E Coli ◽  
Bacteriological Quality

Lack of access to safe drinking water is a threat to public health and wellbeing of the people as it exposes them to risk of waterborne diseases. This study assessed the bacteriological quality of well water samples obtained from Zaria communities and compared the level of contamination between wells with internal wall casting (cast wells) and wells without internal wall casting (non-cast wells). Water samples from 142 wells were collected for a period of six months encompassing both rainy and dry seasons (July to September for rainy season and November to January for dry season). The water samples were collected and analysed for faecal contamination using membrane filtration technique. The water samples contained high density of bacterial counts ranging from 1.73x103±2.73x102 – 2.02x102±1.85x102cfu/100ml during the raining season and 4.17x102±3.07x101 – 1.39x103±3.27x102cfu/100ml during the dry season. Coliforms and E. coli were present in all the samples with  total coliform counts ranging from 1.06 x 103 – 2.12 x 104 cfu/100 ml and E. coli counts ranging from 5.10 X 102 – 5.0 X 104 cfu/100 ml Counts were higher during the raining season in all the locations compared to the dry season. Faecal coliform counts were higher in wells without casting (91.9%) compare to wells with casting (91.2%) while E. coli counts were higher in wells with casting (77.9%) compared to wells without casting (66.2%). There was no significant difference in the bacterial counts between cast and non-cast wells (p-values were all greater than 0.05).A total of 168 organisms were isolated from this study and they include; E. coli(48.3%), Klebsiella pnuemonaie(11.8%), Klebsiella ozanae(13.2%), Enterobacter agglomerans(11.8%), Enterobacter gergoviae(14.7%), Enterobacter aerogenes (8.8%), Citrobacter freundii(8.8%), Proteus mirabilis (5.9%), Serratia liquefaciens(10.3%) and Acinetobacter lwoffii (2.9%). E. coliwas isolated more frequently (48.3%) compared to other species and was more frequent in non-cast wells (50.0%) compared to cast wells (47.1%). Other species were more frequent in cast wells. The bacteria isolated from this study are mostly of enteric origin; these organisms acquire public health significance under certain conditions, therefore, their removal in drinking water should be given moderate priority. The wells in this study fail to meet the minimum standard for safe drinking water and are therefore not safe for domestic purposes especially for drinking purpose without adequate treatment.

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