scholarly journals Ascertaining the Bacteriological Quality of Water Drawn from Cast and Non-cast Wells in Zaria, Nigeria

Author(s):  
Danjuma, Florence Yachim ◽  
Onaji, Ajegba Innocent ◽  
Ocheme Julius Okojokwu ◽  
Danladi Yakubu Papi

Lack of access to safe drinking water is a threat to public health and wellbeing of the people as it exposes them to risk of waterborne diseases. This study assessed the bacteriological quality of well water samples obtained from Zaria communities and compared the level of contamination between wells with internal wall casting (cast wells) and wells without internal wall casting (non-cast wells). Water samples from 142 wells were collected for a period of six months encompassing both rainy and dry seasons (July to September for rainy season and November to January for dry season). The water samples were collected and analysed for faecal contamination using membrane filtration technique. The water samples contained high density of bacterial counts ranging from 1.73x103±2.73x102 – 2.02x102±1.85x102cfu/100ml during the raining season and 4.17x102±3.07x101 – 1.39x103±3.27x102cfu/100ml during the dry season. Coliforms and E. coli were present in all the samples with  total coliform counts ranging from 1.06 x 103 – 2.12 x 104 cfu/100 ml and E. coli counts ranging from 5.10 X 102 – 5.0 X 104 cfu/100 ml Counts were higher during the raining season in all the locations compared to the dry season. Faecal coliform counts were higher in wells without casting (91.9%) compare to wells with casting (91.2%) while E. coli counts were higher in wells with casting (77.9%) compared to wells without casting (66.2%). There was no significant difference in the bacterial counts between cast and non-cast wells (p-values were all greater than 0.05).A total of 168 organisms were isolated from this study and they include; E. coli(48.3%), Klebsiella pnuemonaie(11.8%), Klebsiella ozanae(13.2%), Enterobacter agglomerans(11.8%), Enterobacter gergoviae(14.7%), Enterobacter aerogenes (8.8%), Citrobacter freundii(8.8%), Proteus mirabilis (5.9%), Serratia liquefaciens(10.3%) and Acinetobacter lwoffii (2.9%). E. coliwas isolated more frequently (48.3%) compared to other species and was more frequent in non-cast wells (50.0%) compared to cast wells (47.1%). Other species were more frequent in cast wells. The bacteria isolated from this study are mostly of enteric origin; these organisms acquire public health significance under certain conditions, therefore, their removal in drinking water should be given moderate priority. The wells in this study fail to meet the minimum standard for safe drinking water and are therefore not safe for domestic purposes especially for drinking purpose without adequate treatment.

2019 ◽  
Vol 13 ◽  
pp. 117863021983698 ◽  
Author(s):  
Mercy Mandere Osiemo ◽  
George Morara Ogendi ◽  
Charles M’Erimba

Background: Accessibility to potable water is a fundamental right for dignity and well-being. Despite this observation, more than 1.1 billion people lack access to safe drinking water. This is particularly true in the Sub-Saharan Africa and South East Asia regions. Objective: The main aim of this study was to assess microbial quality of drinking water and prevalence of water-related diseases in Marigat town, Baringo County, Kenya. Methods: Samples of drinking water were collected from water sources (boreholes, rivers, and wells) and at the point of use (households) and analyzed for Escherichia coli and total coliform (TC) bacteria using the most probable number method. In situ measurements of pH and temperature were performed using a Wagtech International portable meter. Clinical health records from the local health centers were also reviewed to assess the prevalence rates of some of the water-related diseases. Results: There were significant differences among water sources during dry season for E coli (F2,21 = 3.629, P < .05) and TC (F2,21 = 4.041, P < .05). Similar observations were made during wet season for E coli (F2,21 = 4.090, P < .05) and TC (F2,21 = 1.893, P < .05). Furthermore, there were significant interactions between the water sources and season for E coli (F2,42 = 7.66, P < .01) and TC (F2,42 = 5.494, P < .05). Drinking water in large plastic storage containers (herein referred to as sky-plast) had the highest E coli and TC concentrations. Typhoid was the most prevalent water-related disease during the dry season (10%), whereas diarrhea (3%) was the most prevalent during the wet season. Conclusions and recommendations: All drinking water at abstraction and point of use for Marigat residents are microbiologically contaminated and therefore pose serious health risks to consumers of such water. Thus, there is need for public health awareness campaigns on household water management to curb incidences of water-related diseases. Public health practitioners at county and national levels need to ensure that households have adequate access to potable water and improved sanitation.


2021 ◽  
Vol 36 (3) ◽  
pp. 47-57
Author(s):  
Waqas Shafiq ◽  
Hadayat Ullah ◽  
Muhammad Zaheer ◽  
Mubashir Mehmood ◽  
Umer Farooq ◽  
...  

The study area is located in the arid section of Punjab province, Pakistan. The accessibility of freshwater is the main problem in this area. The area is mainly flat and alluvial deposits are present down to 800 m. The groundwater is not fit for drinking because of its salinity. Hence, it is essential to mark the transition between fresh water and saltwater. Resistivity models were used to locate freshwater areas. The saltwater areas are between 10 and 30 m deep. Salt flats and clayrich deposits are visible on resistivity maps and were identified by low resistance values. After linking resistivity data and maps, it is determined that the south portion of the area has a freshwater aquifer, especially at a depth of 200 m. At this depth, the quality of water is very good, regardless of its mild salinity in some areas. The margin between salty and freshwater is set to no degree. This is dependent upon the recharge and usage of an aquifer. Groundwater pollution poses a severe threat to the city’s residents. Results from the physio-chemical analysis of the water samples confirm the presence of arsenic in excessive amounts in water samples and are outside the limits set by the WHO and NDWQS. Just two out of 10 samples are within the limits for safe drinking water. The results of this research are valued for groundwater management and the installation of tubular wells in freshwater areas for households and irrigation purposes.


2021 ◽  
Vol 10 (3) ◽  
pp. 214-219

This study was designed to investigate the effect of different thawing methods on the bacteriological quality of frozen broiler chicken meat. Forty-five samples of whole frozen broiler chicken carcasses were collected from different supermarkets in Cairo and Giza governorates. These samples were divided into three groups (fifteen samples for each) as follows: the first group was thawed in a household refrigerator at 7ºC for 20hrs., the 2nd group was thawed over the counter-top at ambient temperature (27-29ºC) for 5-6hrs. while, the 3rd group was thawed in microwave oven for 22 to 24mins. All thawed samples (core temperature arrived at 0ºC) were subjected to bacteriological examination immediately after thawing for enumeration of total aerobic mesophilic bacterial count, Staph. aureus count, total Coliforms count and isolation of food borne pathogens such as E. coli, Salmonella and Staph. aureus. In addition, all isolated bacterial strains were exposed to antimicrobial sensitivity test. The results revealed that the lowest bacterial counts were observed in samples thawed in microwave oven, while the highest bacterial counts were recorded in samples thawed over counter-top. Moreover, Staph. aureus, E. coli and S. kentucky were isolated only from samples thawed over the counter-top and these strains were resistant to amoxicillin and sensitive to fosfomycin. Such results indicated that defrosting in microwave oven is quick and easy method for thawing frozen broiler chicken carcasses and provides an appropriate level of security for the consumers and could achieve the microbiological safety objectives while, thawing over counter-top not recommended due to food safety and quality aspect.


2008 ◽  
Vol 54 (6) ◽  
pp. 495-500 ◽  
Author(s):  
B. Lévesque ◽  
D. Pereg ◽  
E. Watkinson ◽  
J.S. Maguire ◽  
L. Bissonnette ◽  
...  

Bermuda residents collect rainwater from rooftops to fulfil their freshwater needs. The objective of this study was to assess the microbiological quality of drinking water in household tanks throughout Bermuda. The tanks surveyed were selected randomly from the electoral register. Governmental officers visited the selected household (n = 102) to collect water samples and administer a short questionnaire about the tank characteristics, the residents’ habits in terms of water use, and general information on the water collecting system and its maintenance. At the same time, water samples were collected for analysis and total coliforms and Escherichia coli were determined by 2 methods (membrane filtration and culture on chromogenic media, Colilert kit). Results from the 2 methods were highly correlated and showed that approximately 90% of the samples analysed were contaminated with total coliforms in concentrations exceeding 10 CFU/100 mL, and approximately 66% of samples showed contamination with E. coli. Tank cleaning in the year prior to sampling seems to protect against water contamination. If rainwater collection from roofs is the most efficient mean for providing freshwater to Bermudians, it must not be considered a source of high quality drinking water because of the high levels of microbial contamination.


Author(s):  
UBA Uchechukwu Promise ◽  
Dr. Ezekwe Clinton

This study examines the effect of industrial effluents on the water quality of the Aba River. In order to achieve this, a total of six water samples were collected each season and was analysed for different wastewater characteristics of temperature, water pH, Conductivity, Alkaline, Chloride, BOD5, COD, DO, TS, THC, TDS, Zn, Cd, Fe, Pb, Cr, Total Coli-form and E-Coli. Using APHA Standard Analytical Method, the results shows that during dry season, temperature, pH, Chloride and Zn values were found to be lower than NESREA Standard. While TS, THC and Total Coli-form values were found to be higher than NESREA standard in dry season


2016 ◽  
Vol 5 (3) ◽  
Author(s):  
Rani Afrisetiawati ◽  
Erly Erly ◽  
Endrinaldi Endrinaldi

AbstrakAir merupakan  komponen penting bagi tubuh.  Menurut Permenkes No.492 Tahun 2010 tentang syarat mikrobiologi air minum adalah tidak ditemukannya bakteri Coliform dan Escherichia coli dalam 100 ml air. Tujuan penelitian ini adalah mengetahui kualitas air minum yang diproduksi depot air minum isi ulang di Kelurahan Lubuk Buaya. Penelitian bersifat deskriptif dengan melakukan pemeriksaan bakteriologi sampel air di Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas dari Februari  sampai Oktober 2014. Sampel adalah air dari masing-masing depot air minum isi ulang di Kelurahan Lubuk Buaya yang sudah dimasukkan ke dalam galon. Penelitian ini terdiri dari empat tes, yaitu tes presumtif, konfirmatif, lengkap dan identifikasi. Hasil pemeriksaan laboratorium menunjukkan empat dari dua belas sampel (33,3%) mengandung bakteri Coliform dan satu dari empat sampel tersebut mengandung Escherichia coli. Pengawasan terhadap depot air minum isi ulang di Kelurahan Lubuk Buaya harus lebih ditingkatkan. Kader Kesehatan setempat diharapkan dapat memberikan penyuluhan kepada pemilik atau operator depot agar memperhatikan kebersihan depot serta peralatan yang digunakan.Kata kunci: air minum isi ulang, Coliform, Escherichia  coli AbstractWater is an essential component for the human body. According to Permenkes No. 492 Tahun 2010 about microbiological standards for drinking water is the absence of coliform and Escherichia coli in 100 ml of water. The objective of this study was to find the quality of drinking water produced by drinking water refill depots at Lubuk Buaya Padang based on microbiological standards of drinking water.The research was conducted using by bacteriological test on 12 water samples at the Laboratory of Microbiology, Facultyof Medicine, University of Andalas from February until Oktober 2014. This research consisted  four tests: the presumptive test, confirmative test, completetest and identification test. Based on the result of laboratory test, there were four samples (33,3%) from twelve samples contain Coliform bacteria and one of them contain Escherichia coli. Controlling of drinking water refill depots at Lubuk Buaya should have been improved. The officers of Public Health at Lubuk Buaya must give an advice to the owners or depot operators about hygiene of depot and tools that they used.Keywords: drinking water refill, Coliform, Escherichia coli


2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Motuma Tessema Abegaz ◽  
Mulissa Jida Midekssa

The quality of drinking water has always been a major public health concern, especially in developing countries where access to improved water supply and sanitation is very low. This study aimed to assess the bacteriological and physicochemical quality of rural community drinking water sources in the Guto Gida district. A cross-sectional study was conducted in selected rural areas of the district from January to June 2016. Water samples were collected from four types of sources (protected dug well, open dug well, protected spring, and open spring) found in 8 locations of the study area. The membrane filtration technique was employed to determine the total coliform and faecal coliform load of the samples. The physicochemical characteristics such as total dissolved solid (TDS), pH, electrical conductivity (EC), turbidity, temperature, color, iron, manganese, lead, fluoride, zinc, sulphate, nitrate, and phosphate were analyzed following the American Public Health Association and WHO standard protocols. Our results revealed that 90.6% and 87.5% of water samples were positive for total coliform and faecal coliform, respectively. Thus, the majority of the studied water sources could be classified as polluted with respect to coliform load. Our results also have shown that most of the water sources showed marginally tolerable quality with respect to color, EC, TDS, turbidity, nitrate, sulphate, and phosphate. However, the protected sources had poor quality in zinc, lead, iron, manganese, and pH with values above the permissible levels. Thus, the drinking water source quality of the study areas requires appropriate interventions such as improving the existing water source infrastructure and access to sanitation services.


Author(s):  
M. Umar ◽  
J. Kambai ◽  
I. B. Mohammed ◽  
J. O. Oko ◽  
A. A. Obafemi ◽  
...  

Aims: Bacteriological quality assessment and antibiogram profile of bacteria associated with sachet drinking water was carried out with view to determine the bacterial load and fitness of the water sold in the study area for human consumption. Materials and Methods: Ten samples of different brands were aseptically collected and analyzed using heterotrophic count and most probable number technique. The isolated bacteria were microscopically and biochemically characterized and finally confirmed using Box™ Easygel® E. coli Quantitube™ Test Kit (Micrology Laboratories manufactures, Easygel®, USA) biotyping. Physicochemical analysis of the samples was done using standard methods. The isolated bacteria were screened for antimicrobial susceptibility using Kirby-Bauer disc diffusion technique. Results: The total heterotrophic bacterial count ranged from 1.0×102 to 3.0×102 cfu/ml, with MPN/100ml values ranged from <0.03 to 1.2. The temperature ranged from 8 to 18°C, at the pH range of 5.0 to 7.6. The findings showed that, high temperatures and pH was associated with high bacterial counts. There was no statistical relationship between microbial loads and the temperature (p= 0.454) of the analyzed sachet water. Also, there was no statistical relationship between microbial loads and pH (p= 0.446) of the sachet water. The bacteria isolated were Escherichia coli, Enterobacter aerogenes, Salmonella sp, Citrobacter freundii and Proteus vulgaris. Most 9 (90%) of the water analyzed fell within the statutory limits. While, the remaining 1 (10%) fell within the contamination level, which recorded high bacterial counts beyond the standard of safe drinking water set by water and food regulatory bodies. Conclusion: The isolation of E. coli and Salmonella sp. in sample brand “A” in this study is an indication that if not promptly checked, an outbreak could occur in the near future. All the isolates are susceptible to variety of antibiotics used, except Enterobacter aerogenes, Salmonella sp. and Citrobacter freundii which recorded resistance to amoxicillin, and Proteus vulgaris which recorded multidrug resistance.


1970 ◽  
Vol 25 (1) ◽  
pp. 69-72 ◽  
Author(s):  
Abdul Hussain Shar ◽  
Yasmeen Faiz Kazi ◽  
Irshad Hussain Soomro

To assess the bacteriological quality 768 drinking water samples were collected from different locations in Khairpur City, Sindh, Pakistan over a period of two years from January 2006 to December 2007. The study reveals that out of 768 drinking water samples 567 (73.83%) samples were found to be contaminated with total coliform among them 85 (11.06%) found in the January-March period, 182 (23.70%) in April-June period, 188 (24.47%) in July-September, 112 (14.58%) in October-December period. Faecal coliform was found in 351 (45.70%) water sample, in which 49 (15.80%) occurred during January-March, 137 (17.83%) during April-June, 136 (17.71%) during July-September and 69 (8.98%) during September-December. It can be concluded from the results of the present study that bacteriological quality of two-third drinking water in Khairpur City is not safe as the water is the potential source of diarrhoeal disease agents. Keywords: Drinking water; Khairpur City; Faecal pollution; DiarrhoeaDOI: http://dx.doi.org/10.3329/bjm.v25i1.4862 Bangladesh J Microbiol, Volume 25, Number 1, June 2008, pp 69-72


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