Influence of phenylacetic acid pulses on anaerobic digestion performance and archaeal community structure in WWTP sewage sludge digesters

2015 ◽  
Vol 71 (12) ◽  
pp. 1790-1799 ◽  
Author(s):  
Léa Cabrol ◽  
Johana Urra ◽  
Francisca Rosenkranz ◽  
Pablo Araya Kroff ◽  
Caroline M. Plugge ◽  
...  
Keyword(s):  
Community Structure ◽  
Anaerobic Digestion ◽  
Sewage Sludge ◽  
Phenylacetic Acid ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Functional Performance ◽  
Biogas Production ◽  
Treatment Plant ◽  
Archaeal Community ◽  
Methanogenic Activity

The effect of phenylacetic acid (PAA) pulses on anaerobic digestion (AD) performance and archaeal community structure was evaluated in anaerobic digesters treating sewage sludge from a wastewater treatment plant (WWTP). Four pilot-scale continuous stirred tank reactors were set up at a full-scale municipal WWTP in Santiago de Chile, and fed with either primary or mixed sewage sludge. AD performance was evaluated by volatile fatty acid (VFA) and biogas production monitoring. Archaeal community structure was characterized by 16S rRNA denaturing gradient gel electrophoresis and band sequencing. In the primary sludge digester, a single PAA pulse at 200 mg L−1 was sufficient to affect AD performance and archaeal community structure, resulting in long-term VFA accumulation, reduced biogas production and community shift from dominant acetoclastic (Methanosaeta concilii) to hydrogenotrophic (Methanospirillum hungatei) methanogens. By contrast, AD performance and archaeal community structure in the mixed sludge digester were stable and resistant to repeated PAA pulses at 200 and 600 mg L−1. This work demonstrated that the effect of PAA pulses on methanogenic activity and archaeal community structure differed according to AD substrate, and suggests that better insights of the correlations between archaeal population dynamics and functional performance could help to better face toxic shocks in AD.

Potentials and limits of anaerobic digestion of sewage sludge: Energy self-sufficient municipal wastewater treatment plant?

2012 ◽  
Vol 66 (6) ◽  
pp. 1277-1281 ◽  
Author(s):  
P. Jenicek ◽  
J. Bartacek ◽  
J. Kutil ◽  
J. Zabranska ◽  
M. Dohanyos
Keyword(s):  
Wastewater Treatment ◽  
Energy Consumption ◽  
Anaerobic Digestion ◽  
Sewage Sludge ◽  
Wastewater Treatment Plant ◽  
Municipal Wastewater ◽  
Biogas Production ◽  
Treatment Plant ◽  
Self Sufficiency ◽  
Digestion Efficiency

Anaerobic digestion is the only energy-positive technology widely used in wastewater treatment. Full-scale data prove that the anaerobic digestion of sewage sludge can produce biogas that covers a substantial amount of the energy consumption of a wastewater treatment plant (WWTP). In this paper, we discuss possibilities for improving the digestion efficiency and biogas production from sewage sludge. Typical specific energy consumptions of municipal WWTPs per population equivalent are compared with the potential specific production of biogas to find the required/optimal digestion efficiency. Examples of technological measures to achieve such efficiency are presented. Our findings show that even a municipal WWTP with secondary biological treatment located in a moderate climate can come close to energy self-sufficiency. However, they also show that such self-sufficiency is dependent on: (i) the strict optimization of the total energy consumption of the plant, and (ii) an increase in the specific biogas production from sewage sludge to values around 600 L per kg of supplied volatile solids.

scholarly journals Effect of Clinoptilolite and Halloysite Addition on Biogas Production and Microbial Community Structure during Anaerobic Digestion

Materials ◽  
2020 ◽  
Vol 13 (18) ◽  
pp. 4127
Author(s):  
Martyna Ciezkowska ◽  
Tomasz Bajda ◽  
Przemyslaw Decewicz ◽  
Lukasz Dziewit ◽  
Lukasz Drewniak
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Anaerobic Digestion ◽  
Sewage Sludge ◽  
Microbial Community Structure ◽  
Biogas Production ◽  
Oxygen Demand ◽  
Maize Silage ◽  
Gas Treatment ◽  
Significant Difference

The study presents a comparison of the influence of a clinoptilolite-rich rock—zeolite (commonly used for improving anaerobic digestion processes)—and a highly porous clay mineral, halloysite (mainly used for gas purification), on the biogas production process. Batch experiments showed that the addition of each mineral increased the efficiency of mesophilic anaerobic digestion of both sewage sludge and maize silage. However, halloysite generated 15% higher biogas production during maize silage transformation. Halloysite also contributed to a much higher reduction of chemical oxygen demand for both substrates (by ~8% for maize silage and ~14% for sewage sludge) and a higher reduction of volatile solids and total ammonia for maize silage (by ~8% and ~4%, respectively). Metagenomic analysis of the microbial community structure showed that the addition of both mineral sorbents influenced the presence of key members of archaea and bacteria occurring in a well-operated biogas reactor. The significant difference between zeolite and halloysite is that the latter promoted the immobilization of key methanogenic archaea Methanolinea (belong to Methanomicrobia class). Based on this result, we postulate that halloysite could be useful not only as a sorbent for (bio)gas treatment methodologies but also as an agent for improving biogas production.

scholarly journals Archaeal and Bacterial Community Structure in an Anaerobic Digestion Reactor (Lagoon Type) Used for Biogas Production at a Pig Farm

2017 ◽  
Vol 27 (5) ◽  
pp. 306-317 ◽  
Author(s):  
Liliana Pampillón-González ◽  
Nadia L. Ortiz-Cornejo ◽  
Marco Luna-Guido ◽  
Luc Dendooven ◽  
Yendi E. Navarro-Noya
Keyword(s):  
Community Structure ◽  
Anaerobic Digestion ◽  
Biogas Production ◽  
Waste Product ◽  
Archaeal Community ◽  
Pig Manure ◽  
Rrna Gene ◽  
Operational Taxonomic Units ◽  
Sampling Locations ◽  
Pig Farm

Biogas production from animal waste is an economically viable way to reduce environmental pollution and produce valuable products, i.e<i>.</i>, methane and a nutrient-rich organic waste product. An anaerobic digestion reactor for biogas production from pig waste was sampled at the entrance, middle (digestion chamber), and exit of a digester, while the bacterial and archaeal community structure was studied by 16S rRNA gene metagenomics. The number of bacterial operational taxonomic units (OTU)-97% was 3-7 times larger than that of archaeal ones. Bacteria and Archaea found in feces of animals (e.g., Clostridiaceae, Lachnospiraceae, Ruminococcaceae, <i>Methanosarcina</i>, <i>Methanolobus</i>, <i>Methanosaeta</i>, and <i>Methanospirillum</i>) dominated the entrance of the digester. The digestion chamber was dominated by anaerobic sugar-fermenting OP9 bacteria and the syntrophic bacteria <i>Candidatus</i> Cloacamonas (Waste Water of Evry 1; WWE1). The methanogens dominant in the digestion chamber were the acetoclastic <i>Methanosaeta</i> and the hydrogenothrophic <i>Methanoculleus</i> and <i>Methanospirillum</i>. Similar bacterial and archaeal groups that dominated in the middle of the digestion chamber were found in the waste that left the digester. Predicted functions associated with degradation of xenobiotic compounds were significantly different between the sampling locations. The microbial community found in an anaerobic digestion reactor loaded with pig manure contained microorganisms with biochemical capacities related to the 4 phases of methane production.

scholarly journals Inoculum Source Determines Acetate and Lactate Production during Anaerobic Digestion of Sewage Sludge and Food Waste

2019 ◽  
Vol 7 (1) ◽  
pp. 3 ◽  
Author(s):  
Jan Moestedt ◽  
Maria Westerholm ◽  
Simon Isaksson ◽  
Anna Schnürer
Keyword(s):  
Anaerobic Digestion ◽  
Sewage Sludge ◽  
Food Waste ◽  
Methane Production ◽  
Acid Production ◽  
Biogas Production ◽  
Treatment Plant ◽  
Lactate Production ◽  
Total Acid ◽  
Acid Yield

Acetate production from food waste or sewage sludge was evaluated in four semi-continuous anaerobic digestion processes. To examine the importance of inoculum and substrate for acid production, two different inoculum sources (a wastewater treatment plant (WWTP) and a co-digestion plant treating food and industry waste) and two common substrates (sewage sludge and food waste) were used in process operations. The processes were evaluated with regard to the efficiency of hydrolysis, acidogenesis, acetogenesis, and methanogenesis and the microbial community structure was determined. Feeding sewage sludge led to mixed acid fermentation and low total acid yield, whereas feeding food waste resulted in the production of high acetate and lactate yields. Inoculum from WWTP with sewage sludge substrate resulted in maintained methane production, despite a low hydraulic retention time. For food waste, the process using inoculum from WWTP produced high levels of lactate (30 g/L) and acetate (10 g/L), while the process initiated with inoculum from the co-digestion plant had higher acetate (25 g/L) and lower lactate (15 g/L) levels. The microbial communities developed during acid production consisted of the major genera Lactobacillus (92–100%) with food waste substrate, and Roseburia (44–45%) and Fastidiosipila (16–36%) with sewage sludge substrate. Use of the outgoing material (hydrolysates) in a biogas production system resulted in a non-significant increase in bio-methane production (+5–20%) compared with direct biogas production from food waste and sewage sludge.

scholarly journals Enhancement of Methanogenic Activity in Volumetrically Undersized Reactor by Mesophilic Co-Digestion of Sewage Sludge and Aqueous Residue

2021 ◽  
Vol 13 (14) ◽  
pp. 7728
Author(s):  
Vincenzo Torretta ◽  
Athanasia K. Tolkou ◽  
Ioannis A. Katsoyiannis ◽  
Francesca Maria Caccamo ◽  
Marco Carnevale Miino ◽  
...  
Keyword(s):  
Sewage Sludge ◽  
Energy Recovery ◽  
Hydraulic Retention Time ◽  
Biogas Production ◽  
Treatment Plant ◽  
Pilot Scale ◽  
Methanogenic Activity ◽  
Anaerobic Reactors ◽  
Aqueous Residue ◽  
First Time

To date, energy recovery from biological sewage sludge (BSS) by anaerobic digestion has been very popular. However, it can often happen that anaerobic reactors are volumetrically undersized, thus reducing performance in terms of biogas production. A continuous-flow pilot-scale plant was used to investigate, for the first time, the effects of mesophilic anaerobic co-digestion (MACoD) of sewage sludge and aqueous residue (AR) from a biosolids treatment plant (BTP) on methanogenic activity under low hydraulic retention time (HRT) conditions (to simulate the undersizing of the reactor). The results showed that the digestate is always more rapidly biodegradable than the matrices fed, while particulate COD hydrolyzed (12 ± 1.3%) is independent of the quantity of AR dosed. Feeding over 35% of soluble OLR, the total VFAs in the system strongly decreased, despite the low HRT. In correspondence with higher dosages of AR, the percentage of CH4 increased up to 77–78% and the CO2 CH4−1 ratio decreased to 0.25 ± 0.2. Specific methane production increased from 0.09 ± 0.01 m3CH4 kgCODremoved−1 with BSS alone to 0.28 ± 0.01 m3CH4CH4 kgCODremoved−1 in the case of BSS co-digested with AR. Moreover, co-digestion with AR from a BTP allowed continuous specific methanogenic activity to be enhanced from 1.76 ± 0.02 m3CH4 tVSS−1 d−1 to 6.48 ± 0.88 m3CH4 tVSS−1 d−1. Therefore, the MACoD of BSS and AR from a BTP could be a good solution to enhance methanogenic activity in a volumetrically undersized anaerobic digester with reduced HRT.

Sludge minimization by disintegration at different wastewater treatment plants

2008 ◽  
Vol 3 (1) ◽  
Author(s):  
Luchien Luning ◽  
Paul Roeleveld ◽  
Victor W.M. Claessen
Keyword(s):  
Organic Matter ◽  
Wastewater Treatment ◽  
Anaerobic Digestion ◽  
Sewage Sludge ◽  
New Technologies ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Biological Degradation ◽  
Ongoing Research ◽  
Sludge Minimization

In recent years new technologies have been developed to improve the biological degradation of sewage sludge by anaerobic digestion. The paper describes the results of a demonstration of ultrasonic disintegration on the Dutch Wastewater Treatment Plant (WWTP) Land van Cuijk. The effect on the degradation of organic matter is presented, together with the effect on the dewatering characteristics. Recommendations are presented for establishing research conditions in which the effect of sludge disintegration can be determined in a more direct way that is less sensitive to changing conditions in the operation of the WWTP. These recommendations have been implemented in the ongoing research in the Netherlands supported by the National Institute for wastewater research (STOWA).

Simulation of the Impact of Higher Ammonia Recycle Loads Caused by Upgrading Anaerobic Sludge Digesters

2005 ◽  
Vol 40 (4) ◽  
pp. 491-499 ◽  
Author(s):  
Jeremy T. Kraemer ◽  
David M. Bagley
Keyword(s):  
Anaerobic Digestion ◽  
Biogas Production ◽  
Treatment Plant ◽  
Anaerobic Sludge ◽  
Content Increase ◽  
Limited Information ◽  
Dynamic Simulations ◽  
Treatment Train ◽  
Temperature Liquid ◽  
The Impact

Abstract Upgrading conventional single-stage mesophilic anaerobic digestion to an advanced digestion technology can increase sludge stability, reduce pathogen content, increase biogas production, and also increase ammonia concentrations recycled back to the liquid treatment train. Limited information is available to assess whether the higher ammonia recycle loads from an anaerobic sludge digestion upgrade would lead to higher discharge effluent ammonia concentrations. Biowin, a commercially available wastewater treatment plant simulation package, was used to predict the effects of anaerobic digestion upgrades on the liquid train performance, especially effluent ammonia concentrations. A factorial analysis indicated that the influent total Kjeldahl nitrogen (TKN) and influent alkalinity each had a 50-fold larger influence on the effluent NH3 concentration than either the ambient temperature, liquid train SRT or anaerobic digestion efficiency. Dynamic simulations indicated that the diurnal variation in effluent NH3 concentration was 9 times higher than the increase due to higher digester VSR. Higher recycle NH3 loads caused by upgrades to advanced digestion techniques can likely be adequately managed by scheduling dewatering to coincide with periods of low influent TKN load and ensuring sufficient alkalinity for nitrification.

scholarly journals Biochemical Methane Potential of Cork Boiling Wastewater at Different Inoculum to Substrate Ratios

2021 ◽  
Vol 11 (7) ◽  
pp. 3064
Author(s):  
Roberta Mota-Panizio ◽  
Manuel Jesús Hermoso-Orzáez ◽  
Luis Carmo-Calado ◽  
Gonçalo Lourinho ◽  
Paulo Sérgio Duque de Brito
Keyword(s):  
Anaerobic Digestion ◽  
Biogas Production ◽  
Treatment Plant ◽  
Organic Substrates ◽  
Biochemical Methane Potential ◽  
Volatile Solids ◽  
Working Volume ◽  
Methane Potential ◽  
Bmp Test ◽  
Cork Industry

The present study evaluates the digestion of cork boiling wastewater (CBW) through a biochemical methane potential (BMP) test. BMP assays were carried out with a working volume of 600 mL at a constant mesophilic temperature (35 °C). The experiment bottles contained CBW and inoculum (digested sludge from a wastewater treatment plant (WWTP)), with a ratio of inoculum/substrate (Ino/CBW) of 1:1 and 2:1 on the basis of volatile solids (VSs); the codigestion with food waste (FW) had a ratio of 2/0.7:0.3 (Ino/CBW:FW) and the codigestion with cow manure (CM) had a ratio of 2/0.5:0.5 (Ino/CBW:CM). Biogas and methane production was proportional to the inoculum substrate ratio (ISR) used. BMP tests have proved to be valuable for inferring the adequacy of anaerobic digestion to treat wastewater from the cork industry. The results indicate that the biomethane potential of CBWs for Ino/CBW ratios 1:1 and 2:1 is very low compared to other organic substrates. For the codigestion tests, the test with the Ino/CBW:CM ratio of 2/0.7:0.3 showed better biomethane yields, being in the expected values. This demonstrated that it is possible to perform the anaerobic digestion (AD) of CBW using a cosubstrate to increase biogas production and biomethane and to improve the quality of the final digestate.

scholarly journals Biogas potential of organic waste onboard cruise ships — a yet untapped energy source

2021 ◽  
Author(s):  
Kai Schumüller ◽  
Dirk Weichgrebe ◽  
Stephan Köster
Keyword(s):  
Energy Source ◽  
Anaerobic Digestion ◽  
Sewage Sludge ◽  
Food Waste ◽  
Energy Demand ◽  
Organic Waste ◽  
Biogas Production ◽  
Biogas Yield ◽  
Cruise Ships ◽  
Detailed Presentation

AbstractTo tap the organic waste generated onboard cruise ships is a very promising approach to reduce their adverse impact on the maritime environment. Biogas produced by means of onboard anaerobic digestion offers a complementary energy source for ships’ operation. This report comprises a detailed presentation of the results gained from comprehensive investigations on the gas yield from onboard substrates such as food waste, sewage sludge and screening solids. Each person onboard generates a total average of about 9 kg of organic waste per day. The performed analyses of substrates and anaerobic digestion tests revealed an accumulated methane yield of around 159 L per person per day. The anaerobic co-digestion of sewage sludge and food waste (50:50 VS) emerged as particularly effective and led to an increased biogas yield by 24%, compared to the mono-fermentation. In the best case, onboard biogas production can provide an energetic output of 82 W/P, on average covering 3.3 to 4.1% of the total energy demand of a cruise ship.

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