Post-denitrification using alginate beads containing organic carbon and activated sludge microorganisms

2016 ◽  
Vol 74 (7) ◽  
pp. 1626-1635 ◽  
Author(s):  
Dilawar Farhan Shams ◽  
Alexandre Rubio ◽  
Panagiotis Elefsiniotis ◽  
Naresh Singhal
Keyword(s):  
Organic Carbon ◽  
Activated Sludge ◽  
Canola Oil ◽  
Nitrate Removal ◽  
Carbon Sources ◽  
Dairy Farm ◽  
Alginate Beads ◽  
Nitrite Accumulation ◽  
Calcium Tartrate ◽  
Final Effluent

Nitrate concentration in the final effluent is a key issue in pre-denitrification biological treatment systems. This study investigated post-denitrification with alginate beads containing immobilized activated sludge microorganisms and organic carbon source. A batch study was first performed to identify suitable carbon sources among acetate, glucose, calcium tartrate, starch and canola oil on the basis of nitrate removal and bead stability. Canola oil and starch beads exhibited significantly higher denitrification rates, greater bead stability and lower nitrite accumulation (6 mg/L and 10 mg/L, respectively). Glucose and acetate beads showed longer acclimation phases and degraded faster whereas tartrate beads had higher nitrite build-up (39 mg/L) and degraded due to brittleness. Post-denitrification with canola oil and starch beads was investigated in the final clarifier of a coupled upflow bioreactor and aerobic system treating synthetic dairy farm wastewater, and showed a denitrification efficiency of >90%. Beads faded in 12 days due to alginate degradation. Therefore, enhancement in bead strength or use of more stable nontoxic gel would be required to further prolong the treatment. Moreover, this study was conducted at laboratory scale and further research is needed for application in real systems.

Nitrate removal from groundwater using an anoxic-aerobic rotating biological contactor

1996 ◽  
Vol 34 (1-2) ◽  
pp. 323-330 ◽  
Author(s):  
A. Mohseni-Bandpi ◽  
D. J. Elliott
Keyword(s):  
Organic Carbon ◽  
Nitrate Nitrogen ◽  
Nitrate Removal ◽  
Carbon Sources ◽  
Optimum Conditions ◽  
Rotating Biological Contactor ◽  
Nitrite Nitrogen ◽  
In Series ◽  
Rotating Biological Contactors ◽  
Final Effluent

The removal of nitrate-nitrogen from groundwater was investigated using two rotating biological contactors (RBC) in series. The first pilot plant RBC reactor was operated under anoxic condition to remove nitrate-nitrogen. A fraction of effluent of the anoxic RBC was fed to a bench scale aerobic RBC to study the degradation of residual organic carbon and oxidation of nitrite-nitrogen. The first reactor achieved a nitrate removal efficiency of 90 percent for a loading rate of 76 mg/m2.h. The corresponding effluent nitrate, nitrite and residual carbon sources concentrations amounted to 3.3, 0.34 and 3.9 mg/l, respectively. The optimum ethanol to nitrate-nitrogen (E/N) ratio was found to be 2.35. No residual ethanol and nitrite-nitrogen were observed in the final effluent under optimum conditions. Dissolved oxygen in the final effluent was found to be greater than 7 mg/l. The results of the study suggest that using a continuous anoxic-aerobic RBC is a convenient and reliable process for removal of nitrate, residual organic carbon and nitrite.

Increasing the capacity for treatment of chemical plant wastewater by replacing existing suspended carrier media with Kaldnes Moving Bed(tm) media at a plant in Singapore

2004 ◽  
Vol 49 (11-12) ◽  
pp. 199-205 ◽  
Author(s):  
F.G. Wessman ◽  
E. Yan Yuegen ◽  
Q. Zheng ◽  
G. He ◽  
T. Welander ◽  
...  
Keyword(s):  
Organic Carbon ◽  
Activated Sludge ◽  
Normal Operation ◽  
Organic Load ◽  
Moving Bed ◽  
Biofilm Carrier ◽  
L 51 ◽  
Biofilm Process ◽  
Suspended Carrier ◽  
Final Effluent

The Kaldnes biomedia K1, which is used in the patented Kaldnes Moving Bed(tm) biofilm process, has been tested along with other types of biofilm carriers for biological pretreatment of a complex chemical industry wastewater. The main objective of the test was to find a biofilm carrier that could replace the existing suspended carrier media and at the same time increase the capacity of the existing roughing filter-activated sludge plant by 20% or more. At volumetric organic loads of 7.1 kg COD/m3/d the Kaldnes Moving Bed™ process achieved much higher removal rates and much lower effluent concentrations than roughing filters using other carriers. The Kaldnes roughing stage achieved more than 85% removal of organic carbon and more than 90% removal of BOD5 at the tested organic load, which was equivalent to a specific biofilm surface area load of 24 g COD/m2/d. Even for the combined roughing filter-activated sludge process, the Kaldnes carriers outperformed the other carriers, with 98% removal of organic carbon and 99.6% removal of BOD5. The Kaldnes train final effluent concentrations were only 22 mg FOC/L and 7 mg BOD5/L. Based on the successful pilot testing, the full-scale plant was upgraded with Kaldnes Moving Bed™ roughing filters. During normal operation the upgraded plant has easily met the discharge limits of 100 mg COD/L and 50 mg SS/L. For the month of September 2002, with organic loads between 100 and 115% of the design load for the second half of the month, average effluent concentrations were as low as 9 mg FOC/L, 51 mg COD/L and 12 mg SS/L.

scholarly journals Glycerol-driven Denitratation: Process Kinetics, Microbial Ecology, and Operational Controls

2021 ◽  
Author(s):  
Matthew P. Baideme ◽  
Chenghua Long ◽  
Luke T. Plante ◽  
Jeffrey A. Starke ◽  
Michael A. Butkus ◽  
...  
Keyword(s):  
Organic Carbon ◽  
Microbial Ecology ◽  
Nitrate Reduction ◽  
Carbon Sources ◽  
Selective Reduction ◽  
Nitrite Reduction ◽  
Nitrite Accumulation ◽  
Ammonium Oxidation ◽  
Order Of Magnitude ◽  
Nitrate And Nitrite

ABSTRACTDenitratation, the selective reduction of nitrate to nitrite, is a novel process when coupled with anaerobic ammonium oxidation (anammox) could achieve resource-efficient biological nitrogen removal of ammonium- and nitrate-laden waste streams. Using a fundamentally-based, first principles approach, this study optimized a stoichiometrically-limited, glycerol-driven denitratation process and characterized mechanisms supporting nitrite accumulation with results that aligned with expectations. Glycerol supported selective nitrate reduction to nitrite and near-complete nitrate conversion, indicating its viability in a denitratation system. Glycerol-supported specific rates of nitrate reduction (135.3 mg-N/g-VSS/h) were at least one order of magnitude greater than specific rates of nitrite reduction (14.9 mg-N/g-VSS/h), potentially resulting in transient nitrite accumulation and indicating glycerol’s superiority over other organic carbon sources in denitratation systems. pH and ORP inflection points in nitrogen transformation assays corresponded to maximum nitrite accumulation, indicating operational setpoints to prevent further nitrite reduction. Denitratation conditions supported enrichment of Thauera sp. as the dominant genus. Stoichiometric limitation of influent organic carbon, coupled with differential nitrate and nitrite reduction kinetics, optimized operational controls, and a distinctively enriched microbial ecology, was identified as causal in glycerol-driven denitratation.

scholarly journals Post-Treatment of the Effluent from Anaerobic Digestion of the Leachate in Two-Stage SBR System Using Alternative Carbon Sources

2021 ◽  
Vol 13 (11) ◽  
pp. 6297
Author(s):  
Katarzyna Bernat ◽  
Dorota Kulikowska ◽  
Magdalena Zielińska ◽  
Magdalena Zaborowska ◽  
Irena Wojnowska-Baryła ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Nitrate Removal ◽  
Carbon Sources ◽  
Post Treatment ◽  
Effluent Treatment ◽  
Electricity Production ◽  
Nitrite Accumulation ◽  
Ammonium Oxidation ◽  
Two Stage ◽  
Removal Rates

Although anaerobic digestion (AD) enables biogas production and facilitates renewable electricity production, its effluent must be post-treated before discarding it into the environment. However, during AD designing, the post-treatment step is often overlooked. This paper presents the kinetics and efficiency of nitrogen removal from effluent after AD of leachate from the aerobic stabilization of the organic fraction of municipal solid waste. A two-stage SBR system was used. An ammonium oxidation rate of 15.5 mg N-NH4/(L·h) ensured a 98% nitrification efficiency (I stage). For denitrification (II stage), alternative carbon sources (ACS) (molasses, crude glycerine, or distillery stillage) were used. Two volumetric exchange rates (n) were tested: 0.35 1/d (COD/N-NO3 ratio of 8) and 0.5 1/d (COD/N-NO3 of 7). With all ACS and COD/N-NO3 ratios, almost 100% of nitrate was denitrified; at the COD/N-NO3 of 8, biodegradable organics remained in the effluents. At the COD/N-NO3 of 7, the denitrification removal rates were lower (29.6-45.1 mg N-NOx/(L·h)) than at the ratio of 8 (72.1–159.5 mg N-NOx/(L·h)), because of temporal nitrite accumulation. The highest nitrate removal rates were obtained with molasses, the lowest with a distillery stillage. Considering the nitrate removal rate and the effluent COD concentration, molasses was recommended as the most effective carbon source for AD effluent treatment at the COD/N-NO3 of 7.

Pyridine Degradation by Suspensions and Biofilms of Achromobacter pulmonis PNOS and Burkholderia dolosa BOS Strains Isolated from Activated Sludge of Sewage Treatment Plants

2020 ◽  
Vol 36 (2) ◽  
pp. 86-98
Author(s):  
A.A. Sergeeva ◽  
G.V. Ovechkina ◽  
A.Yu. Maksimov
Keyword(s):  
Activated Sludge ◽  
Sewage Treatment ◽  
Local Treatment ◽  
Carbon Sources ◽  
Mining Institute ◽  
Bacterial Strains ◽  
Federal Research ◽  
Registration Number ◽  
Treatment Facilities ◽  
Pyridine Degradation

Bacterial strains capable of degradation of 0.8-15.8 g/1 pyridine hydrochloride have been isolated from activated sludge of municipal biological treatment plants in Perm (BOS) and local treatment facilities of the LUKOIL-Permnefteorgsintez enterprise (PNOS). The strains were identified as Achromobacter pulmonis and Burkholderia dolosa. The optimal pyridine concentration for the growth of the isolated strains was 4.0 g/1. The pyridine degradation during the A. pulmonis PNOS and B. dolosa BOS cultivation on a medium with ammonium chloride and glucose and without additional nitrogen or carbon sources was studied. It was shown that the strains are able to accumulate biomass in a medium with pyridine as the sole carbon and nitrogen source; the addition of glucose to the medium (1 g/L) accelerated the pyridine degradation by A. pulmonis PNOS, but inhibited the process carried out by B. dolosa BOS. B. dolosa BOS and A. pulmonis PNOS biofilms efficiently utilized pyridine during growth on basalt and carbon fibers; the highest rate of pyridine utilization (1.8 g /(L day)) was observed in A. pulmonis PNOS biofilms on basalt fibers. pyridine, biodegradation, activated sludge, biofilms, Achromobacter pulmonis, Burkholderia dolosa The authors grateful to Dr. I.I. Tchaikovsky, Head of the Laboratory of Geology of Mineral Deposits of the Mining Institute, a branch of the Perm Federal Research Center, for help with electron microscopy of the samples. This work was carried out as part of a state assignment on the topic « Study of the Functional and Species Diversity of Microorganisms Useful for Ecocenoses and Human Practical Activity», registration number R&D AAAA-A19-119112290008-4.

Parameters for Measuring the Activity and Viability of Activated Sludge

1974 ◽  
Vol 9 (1) ◽  
pp. 235-249 ◽  
Author(s):  
S.G. Nutt ◽  
K.L. Murphy
Keyword(s):  
Organic Carbon ◽  
Activated Sludge ◽  
Biological System ◽  
Analytical Methods ◽  
Heterogeneous System ◽  
Removal Rate ◽  
Bacterial Species ◽  
Atp Content ◽  
Culture Studies ◽  
Activated Sludge System

Abstract Conventional wastewater parameters are accepted as inadequate estimates of the condition of activated sludge but numerous other indices have been suggested as specific measurements of the activity and viability of the biomass. Literature in the related fields of microbiology and biochemistry were reviewed in order to select the most appropriate activity parameters for application to a heterogeneous biological material. Modified analytical methods were applied to a well-controlled biological system containing a single predominant bacterial species to evaluate the relative merit of each as an indicator of viability and activity. The potential of each parameter in a complex heterogeneous system was determined by monitoring each index in a bench activated sludge system. The predominant culture studies indicated that the ATP content of the biomass and the dehydrogenase activity were potential indicators of cell viability in a simple system. However, in the complex activated sludge system, only the ATP content showed significant correlation to the organic carbon removal rate.

Assimilation of various organic carbon sources by Haematococcus strains

2009 ◽  
Vol 57 (2) ◽  
pp. 231-237
Author(s):  
M. Zych ◽  
A. Stolarczyk ◽  
K. Maca ◽  
A. Banaś ◽  
K. Termińska-Pabis ◽  
...  
Keyword(s):  
Organic Carbon ◽  
Organic Compounds ◽  
Present Experiment ◽  
Carbon Sources ◽  
Growth Conditions ◽  
Mixotrophic Growth ◽  
Naturally Occurring ◽  
Organic Carbon Sources ◽  
Secondary Carotenoids ◽  
Colour Changes

Differences in the assimilation of individual organic compounds (5 mM sugars and L-asparagine) under mixotrophic growth conditions were described for three naturally occurring Haematococcus strains.The effects of assimilation were measured by the growth intensity and size of algal cells, and the effect of colour changes in the cultures was observed. Some compounds caused the cell colouration to change from green to yellow, being the result of chlorophyll disappearance and the accumulation of yellow secondary carotenoids. In the present experiment none of the cultures turned red, thus excluding the intense accumulation of the commercially interesting carotenoid, astaxanthin.

The vulnerability of Arctic shelf sediments to climate change

2015 ◽  
Vol 23 (4) ◽  
pp. 461-479 ◽  
Author(s):  
Robie W. Macdonald ◽  
Zou Zou A. Kuzyk ◽  
Sophia C. Johannessen
Keyword(s):  
Organic Carbon ◽  
Carbon Sources ◽  
The Arctic ◽  
Sedimentary Environments ◽  
Carbon Supply ◽  
Shelf Sediment ◽  
Wide Range ◽  
Metabolic Conditions ◽  
Shelf Sediments ◽  
Ocean Ecosystem

The sediments of the pan-Arctic shelves contribute an important component to the Arctic Ocean ecosystem by providing a habitat for biota (benthos), a repository for organic and inorganic non-conservative substances entering or produced within the ocean, a reactor and source of transformed substances back to the water column, and a mechanism of burial. Sediments interact with ice, ocean, and the surrounding land over a wide range of space and time scales. We discuss the vulnerability of shelf sediment to changes in (i) organic carbon sources, (ii) pathways of sediment and organic carbon supply, and (iii) physical and biogeochemical alteration (diagenesis). Sedimentary environments of the shelves and basins are likely to exhibit a wide variance in their response to global change because of their wide variation in sediment sources, processes, and metabolic conditions. In particular, the Chukchi and Barents shelves are dominated by inflowing waters from oceans to the south, whereas the interior shelves are more closely tied to terrigenous sources due to river inflow and coastal erosion.

Evaluation of hybrid processes for nitrification by comparing MBBR/AS and IFAS configurations

2007 ◽  
Vol 55 (8-9) ◽  
pp. 43-49 ◽  
Author(s):  
E. Germain ◽  
L. Bancroft ◽  
A. Dawson ◽  
C. Hinrichs ◽  
L. Fricker ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Biofilm Reactor ◽  
Nitrite Accumulation ◽  
Removal Rates ◽  
Solids Retention ◽  
Different Temperatures ◽  
Suspended Biomass ◽  
The Media ◽  
Population Counts ◽  
Ammonia Oxidising Bacteria

An integrated fixed-film activated sludge (IFAS) pilot plant and a moving bed biofilm reactor coupled with an activated sludge process (MBBR/AS) were operated under different temperatures, carbon loadings and solids retention times (SRTs). These two types of hybrid systems were compared, focusing on the nitrification capacity and the nitrifiers population of the media and suspended biomass alongside other process performances such as carbonaceous and total nitrogen (TN) removal rates. At high temperatures and loadings rates, both processes were fully nitrifying and achieved similarly high carbonaceous removal rates. However, under these conditions, the IFAS configuration performed better in terms of TN removal. Lower temperatures and carbon loadings led to lower carbonaceous removal rates for the MBBR/AS configuration, whereas the IFAS configuration was not affected. However, the nitrification capacity of the IFAS process decreased significantly under these conditions and the MBBR/AS process was more robust in terms of nitrification. Ammonia oxidising bacteria (AOB) and nitrite oxidising bacteria (NOB) population counts accurately reflected the changes in nitrification capacity. However, significantly less NOBs than AOBs were observed, without noticeable nitrite accumulation, suggesting that the characterisation method used was not as sensitive for NOBs and/or that the NOBs had a higher activity than the AOBs.

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