scholarly journals Fluid-transport evaluation of lateral condensation, GuttaCore™ and continuous wave of obturation techniques

RSBO ◽  
2017 ◽  
Vol 13 (3) ◽  
pp. 171
Author(s):  
Daniel Silva-Herzog ◽  
Ezequiel Monroy ◽  
Verónica Méndez ◽  
Sandra Maria Alves Sayão Maia ◽  
Paulo Melo Júnior ◽  
...  
Keyword(s):  
Transport System ◽  
Fluid Transport ◽  
Continuous Wave ◽  
Positive Control ◽  
Control Group ◽  
Suitable Material ◽  
Root Canals ◽  
Nail Polish ◽  
Working Length ◽  
Apical Leakage

To achieve success in endodontic treatment, the root canal system should be as sealed as possible with suitable material such as gutta-percha and sealer. Objective: This study compare the apical leakage of roots obturated with GuttaCore™, lateral condensation, and continuous wave obturation through a computerized fluid-transport system. Material and methods: Fifty-two freshlyextracted upper molars were used. The disto-buccal roots were cut and standardized to 10 mm long. The root canals were prepared at working length with WaveOne primary reciprocating files. The specimens were randomly divided and filled by one of the following obturation techniques: GuttaCore™, lateral condensation or continuous wave obturation, using SILCO sealer. The positive controls were left unfilled and the negative controls were totally coated with cyanoacrylate and three layers of nail polish. The roots were stored in relative humidity for 72 h at 37°C, allowing the sealer to set. After this period, the roots were connected to a computerized fluid-transport system, and the apical leakage was analyzed. Results: The results were expressed in µL.cmH2O-1.min-1 x10-4 1.36 atm. The data were statistically analyzed using one-way ANOVA and Tukey´s tests. The specimens of the positive control group showed extreme amounts of apical leakage. There was no leakage in the negative ontrols. The statistical analysis indicated that continuous wave obturation and GuttaCore™ showed lower leakage than the lateral condensation technique (p<0.05). No difference was found among the continuous wave obturation and GuttaCore™ (p>0.05). Conclusion: Continuous wave obturation and GuttaCore™ showed lower leakage than the lateral condensation technique. There was no difference between the continuous wave obturation and GuttaCore™. The fluid-transport system used in this study allowed an accurate quantitative measurement of leakage using simpler equipment.

scholarly journals Evaluation of the Sealing Ability of Three Obturation Techniques Using a Glucose Leakage Test

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Katarzyna Olczak ◽  
Halina Pawlicka
Keyword(s):  
Continuous Wave ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Thermal Methods ◽  
Root Canals ◽  
Anterior Teeth ◽  
Sealing Ability ◽  
Significant Difference

The aim of this study was to evaluate the sealing ability of three different canal filling techniques. Sixty-four roots of extracted human maxillary anterior teeth were prepared using ProTaper® rotary instruments. The specimens were then randomly divided into 3 experimental groups (n=16) and 2 control groups (n=8). The root canals were filled using cold lateral compaction (CLC group), continuous wave condensation technique using the Elements Obturation Unit® (EOU group), and ProTaper obturators (PT group). For the negative control group, 8 roots were filled using lateral compaction as in the CLC group, and the teeth were covered twice with a layer of nail varnish (NCG group). Another 8 roots were filled using lateral compaction, but without sealer, and these were used as the positive control (PCG group). A glucose leakage model was used for quantitative evaluation of microleakage for 24 hours and 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12 weeks. No significant difference in the cumulative amount of leakage was found between the three experimental groups at all observation times. The lateral condensation of cold gutta-percha can guarantee a similar seal of canal fillings as can be achieved by using thermal methods, in the round canals.

scholarly journals Evaluation of Apical Leakage of Root Canals Obturated with Nd: YAG Laser Softened Gutta-Percha, System-B, and Lateral Condensation Techniques

2002 ◽  
Vol 3 (1) ◽  
pp. 27-42 ◽  
Author(s):  
Murat Maden ◽  
Güliz Görgül ◽  
A. Cemal Tinaz
Keyword(s):  
Heat Source ◽  
Nd:Yag Laser ◽  
Continuous Wave ◽  
Root Canals ◽  
Single Root ◽  
Gutta Percha ◽  
Working Length ◽  
Significant Difference ◽  
Length Determination ◽  
Apical Leakage

Abstract In endodontics, a laser can be used as a heat source and gutta-percha can be softened by a laser and used in the root canals. The System-B endodontic heat source unit was designed for the obturation of the root canal system with a single continuous wave of thermoplasticized gutta-percha. The aim of this study was to compare the apical leakage of lateral condensation; Nd:YAG laser-softened gutta-percha and System-B techniques. Fifty-five extracted, single root human maxillary anterior and premolar teeth were used in this study. After working length determination, the “step-back technique” was used to prepare the root canals. The teeth were randomly divided into three experimental groups of 15 each and two control groups of 5 specimens each. The first group was obturated by lateral condensation, the second group by gutta-percha softened with an Nd:YAG laser, and the third group was obturated using the system-B technique. Apical leakage of the roots was evaluated by dye penetration using a stereomicroscope after sectioning the roots. The results of the first and third groups were similar, and the results of the second group showed differences when compared with the other groups. However, variance analysis revealed there was no statistically significant difference among the results. Citation Maden M, Görgül G, Tinaz AC. Evaluation of Apical Leakage of Root Canals Obturated with Nd:Yag laser-softened Gutta-percha, System-B, and Lateral Condensation Techniques. J Contemp Dent Pract 2002 Feb;(3)1: 016-026.

scholarly journals A Digital Microscopic Inspection of Dentinal Defects after Using Endodontic Retreatment Files

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Ammar AbuMostafa ◽  
Hala Almoqayyad ◽  
Al-Omari Mohammad
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Filling Material ◽  
Root Canals ◽  
Root Canal Retreatment ◽  
Significant Difference ◽  
Digital Microscope ◽  
Microscopic Inspection

Aim. The study aimed at evaluating the incidence of dentinal defects after root canal retreatment with ProTaper Universal retreatment (PTUR) and XP-endo Shaper and Finisher R (XP). Materials and Methods. Sixty extracted single-rooted human premolars were selected and divided into 4 groups of 15 teeth each. In the negative control group, the teeth were left unprepared. In the positive control group, the teeth were prepared with ProTaper Next and obturated with no further retreatment. In the PTUR and XP groups, the teeth were prepared and obturated followed by removal of the filling material at body temperature using PTUR and XP instruments, respectively. The roots were then sectioned at 3, 6, and 9 mm from the apex and observed under a digital microscope to detect defects. Results. PTUR group showed significantly higher ( p value <0.05) incidence of defects than the other groups. Comparison of no defects versus defects between groups in different areas of root canals demonstrated significant difference among the groups in the apical and cervical regions. Conclusion. Within the limitations of the present study, PTUR files created significantly more dentinal defects than XP files, with most of those defects at the cervical and apical areas of the root canals.

scholarly journals COMPARISON EFFECT OF CV 12, ST 36 AND ST 40 EA ON SHORT TERM ENERGY BALANCE REGULATION IN HIGH FAT DIET RAT

2017 ◽  
Vol 52 (3) ◽  
pp. 174
Author(s):  
Purwo Sri Rejeki ◽  
Harjanto Harjanto ◽  
Raden Argarini ◽  
Imam Subadi
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Energy Balance ◽  
High Fat Diet ◽  
Energy Homeostasis ◽  
Continuous Wave ◽  
Positive Control ◽  
Control Group ◽  
High Fat ◽  
Short Term

The aim of this study was to determine the comparative effects of EA (EA) on the CV12, ST36 and ST40 to weight gain prevention over the short-term regulation of energy balance. The study was conducted with a completely randomized design. Rats were divided into five groups: negative control group (no treatment, n=5), positive control (sham EA/back, n=5), EA CV 12 (n=6), EA ST 36 (n=6) and EA ST 40 (n=7). Rats were exposed to high-fat diet for two weeks and EA was simultaneously performed once daily, five days a week for two weeks with 2 Hz, for 10 minutes with continuous wave. Body weight, BMI, front limb circumference and rear were measured during study. Levels of blood glucose, cholesterol, triglycerides, LDL and HDL were measured at the end of the study; which reflects the short-term regulation of energy homeostasis. For weight loss, EA CV12, ST36 and ST40 group have lost weight significantly compared to the negative and positive control group. The ST40 group has a significant decrease than ST36 and CV12. The most significant decrease in BMI found in the ST40 group. EA did not affect blood glucose levels, but modulated blood lipid profile. In ST 40 group there was a significant decrease in cholesterol, LDL and triglycerides. EA at point ST 40 is potential in preventing increased body weight and BMI in rats exposed to high-fat diet compared to the CV 12 and ST 36. ST 40 is a point with a potential of lowering LDL and triglycerides serum so that it can play a role in the short term regulation of energy homeostasis but also in the prevention of dyslipidemia.

Antibacterial Efficacy of Diode and Er:YAG Laser Irradiation in Experimentally Contaminated Primary Molar Root Canals

2009 ◽  
Vol 34 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Senem Selvi Kuvvetli ◽  
Nuket Sandalli ◽  
Nursen Topcuoglu ◽  
Guven Kulekci
Keyword(s):  
Laser Irradiation ◽  
Diode Laser ◽  
Er:Yag Laser ◽  
Positive Control ◽  
Control Group ◽  
Antibacterial Efficacy ◽  
Primary Molar ◽  
Root Canals ◽  
Number Of Bacteria

Objective: In vitro comparison of the antibacterial efficacy of Diode and Er:YAG laser irradiation with that of NaOCl irrigation in contaminated primary molar root canals. Study Design: 96 root canals prepared from 32 extracted primary molar teeth were mechanically enlarged and the teeth were randomly divided into 4 subgroups. The roots were inoculated with an overnight culture of Enterococcus faecalis in tryptic soy broth for 24 hours. The root canals irradiated with diode and Er:YAG laser and irrigated with NaOCl(5.25%) were experimental groups and untreated canals served as positive control group. Bacterial growth was analysed by counting viable E.faecalis on tryptic soy agar plates. Results: The number of bacteria was significantly reduced in experimental groups in comparison with the control group. Diode laser was determined to be more effective in reducing the number of bacteria when compared to Er:YAG laser. NaOCl irrigation was found significantly most effective. Conclusions: Diode laser irradiation and 5.25 % NaOCl application provided a significant antibacterial effect in vitro, in contaminated primary molar root canals.

Microbial Leakage of Cavit, IRM, and Temp Bond in Post-prepared Root Canals Using Two Methods of Gutta-percha Removal: An In Vitro Study

2005 ◽  
Vol 6 (3) ◽  
pp. 53-61 ◽  
Author(s):  
Hanan Balto ◽  
Saad Al-Nazhan ◽  
Khulood Al-Mansour ◽  
Moneera Al-Otaibi ◽  
Yunus Siddiqu
Keyword(s):  
Root Canal ◽  
Bacterial Suspension ◽  
Control Group ◽  
Endodontically Treated Teeth ◽  
Root Canals ◽  
Gutta Percha ◽  
Working Length ◽  
Significant Difference ◽  
Post Space

Abstract The aim of this study was to evaluate the integrity of the coronal seal of Temp-Bond and compare it to Cavit and IRM after post space preparation using S. faecalis as a microbial tracer. In addition, the affect of two methods of gutta percha removal on the apical seal of root canal fillings was also evaluated. Forty extracted human single rooted teeth were prepared chemomechanically and obturated with gutta percha and AH26 sealer cement using the lateral cold condensation technique to a standardized working length of 15 mm. About 10 mm of the coronal gutta-percha was removed with either Peeso-reamer or a hot plugger. The roots were divided into three experimental groups of 10 roots and a control group. Each experimental group was subdivided equally into two groups of 15 each according to the method of post space preparation. Cavit, IRM, and Temp-Bond were used to seal the access opening. Each root was fixed in a cuvette containing Tryptic Soya Broth which, covered 2 mm of the root apex. Bacterial suspension was introduced through pipette. Fresh bacterial suspension was added every week, and the system was monitored daily for the growth of microorganisms for a period of one month. The results showed there was no significant difference in terms of coronal leakage between the three coronal materials used (P=0.478), but the methods of gutta-percha removal did have an impact on the apical leakage (P=0.047). The mean value showed the Peeso-reamer provided less leakage compared to using a hot plugger during the 30-day experimental time period. It was concluded the temporary type of coronal seal of endodontically treated teeth will not prevent coronal leakage if left for a long period of time. In addition, permanent cementation of the post with the coronal restoration should be carried out as soon as possible to prevent recontamination of the root canal. Citation Balto H, Al-Nazhan S, Al-Mansour K, Al-Otaibi M, Siddiqu Y. Microbial Leakage of Cavit, IRM, and Temp Bond in Post-prepared Root Canals Using Two Methods of Gutta-percha Removal: An In Vitro Study. J Contemp Dent Pract 2005 August;(6)3:053-061.

scholarly journals Assessment of apical microleakage using tooth clearing method between two softened core obturation techniques

2016 ◽  
Vol 1 (1) ◽  
pp. 39
Author(s):  
Nadia S. Wongsari ◽  
Christine A. Rovani ◽  
Aries C. Trilaksana
Keyword(s):  
Root Canal ◽  
Continuous Wave ◽  
Positive Control ◽  
Single Root ◽  
The Third ◽  
Gutta Percha ◽  
Nail Polish ◽  
Wave Technique ◽  
Post Hoc ◽  
Clearing Method

This study aimed to compare apical microleakage using tooth clearing method between two softened core obturation techniques which are carrier based gutta percha and continuous wave. Samples are maxillary first incisors, with a single root canal. They are devided into three groups of 9 each randomly. The first group is carrier based gutta percha, the second is continuous wave, and the third is lateral condensation as positive control. The teeth were decoronated at the cementoenamel junction, prepared with crown down pressureless technique, and obturated according their group.  All the samples were coated with nail polish except for 2 mm from the apical, and then placed in China Ink for 7 days. After that, the polish were removed and the samples undergone tooth clearing procedure. When the samples had been transparant, they were observed using microscop. Data were collected and analyzed using Anova and Tukey’s post hoc test. The study showed that mean of apical microleakage from continuous wave technique (0.256+0.133) is less than from carrier based gutta percha (0.433+0.173). As the conclusion, there is a significant diffrence (p:0.034) of apical microleakage between continuous wave and carrier based gutta percha (p<0.05).

scholarly journals The influence of working length on the reduction of biofilm and planktonic bacteria in oval canals with reciprocating instrumentation

2021 ◽  
Vol 10 (5) ◽  
pp. e9810514765
Author(s):  
Arieth Cristina Sacomani ◽  
Fernanda Tessaro Cintra ◽  
Adriana de Jesus Soares ◽  
Marcos Frozoni
Keyword(s):  
Bacterial Growth ◽  
Root Canal ◽  
Negative Control ◽  
Bacterial Biofilm ◽  
Control Group ◽  
Root Canal Preparation ◽  
Root Canals ◽  
Planktonic Bacteria ◽  
Working Length ◽  
Significant Difference

To evaluate the influence of reciprocating single-file instrumentation with different working lengths (WL) on the reduction of planktonic bacteria and bacterial biofilm in Enterococcus faecalis-contaminated oval root canals. Methodology: Fifty-five human single-rooted canines were used. Fifty were inoculated with E. faecalis for 21 days for biofilm formation. To confirm the formation of biofilm adhered to the root canal wall, 5 contaminated samples from positive control group were analyzed by SEM. Samples were assigned into 3 groups (n = 15) according to working length determined, G+1 root canal preparation 1 mm beyond the apical foramen, G0 root canal preparation at the major foramen, and G-1 root canal preparation 1 mm short of the major foramen. Five roots were not inoculated to serve as a negative control. Bacteriological samples were collected prior to preparation, initial collection (S1), and after reciprocating instrumentation (S2) by disaggregating biofilm to quantify the reduction of planktonic bacteria and intracanal biofilm at different WL. Bacterial quantitation was performed using colony-forming units per milliliter (CFU / mL) count. Statistical analysis was performed at the significance level of 0.05. Results: No bacterial growth was observed in the negative control. All positive controls demonstrated bacterial growth; S1 from all teeth were positive for bacteria with no significant difference. The post-hoc analysis showed G+1 promoting a significantly higher disinfection than G-1 (p<0,05) and G-1 similar disinfection to G0 (P=962). Conclusion: Instrumentation as close as possible to major foramen or beyond it improves decontamination in oval root canals with reciprocating instrumentation.

scholarly journals Comparison of Antibacterial Effects of Photodynamic Therapy, Modified Triple Antibiotic Paste and Calcium Hydroxide on Root Canals Infected With Enterococcus faecalis: An In Vitro Study

2019 ◽  
Vol 10 (5) ◽  
pp. S23-S29 ◽  
Author(s):  
Mohammad Asnaashari ◽  
Mohammad Jafar Eghbal ◽  
Amirali Sahba Yaghmayi ◽  
Mehdi Shokri ◽  
Saranaz Azari-Marhabi
Keyword(s):  
Photodynamic Therapy ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Control Group ◽  
Root Canals ◽  
Anterior Teeth ◽  
Triple Antibiotic Paste ◽  
Working Length

Introduction: A pivotal issue to achieve success in the treatment of the root canal is root canal disinfection. One of the most important bacteria that infect the root canal is Enterococcus faecalis. This study seeks to examine the effectiveness of 3 methods for disinfecting the root canal: photodynamic therapy, modified triple antibiotic paste (MTAP), and calcium hydroxide. Methods: Sixty-two single-rooted extracted anterior teeth were collected. After cleaning and disinfecting the teeth, their crowns were cut at the CEJ point. The root canals were shaped to the working length up to file F3 ProTaper (F1, F2, F3). EDTA 17% and sodium hypochlorite 2.5% were used for 5 minutes to wash and remove the smear layer, and then the apical foramen was sealed using composite. After that, the teeth were sterilized in an autoclave at 121°C for 15 minutes. Then 10 samples were taken randomly as the negative control. The remaining samples were immersed and cultivated in a suspension containing E. faecalis for 21 days. Then the samples were divided into 5 groups: 2 positive control groups, 1 group treated with the antibiotic paste with the concentration of 1 mg/mL, 1 group treated with calcium hydroxide, and 1 group treated with photodynamic therapy. Then, to collect the biofilm, the ProTaper file F4 was used. After that, the microbial suspension was provided and counting the colonies was carried out to compare the groups. Results: The findings indicated that the amount of CFU/mg of MTAP samples, including clindamycin, metronidazole, ciprofloxacin in the concentration of 1 mg/mL and photodynamic therapy and calcium hydroxide was lower than that in the control group. Antibiotic paste cleansed the root canal up to 99.9%. Photodynamic therapy reduced the amount of CFU/mg to 98.8%, and calcium hydroxide reduced the amount of CFU/mg to 94.13%. Conclusion: Using photodynamic therapy causes a reduction in the biofilm and inhibits the growth of the E. faecalis bacterium. In addition, in this study, MTAP with a concentration of 1 mg/mL was used, which expunged the bacteria completely. Meanwhile, calcium hydroxide had the weakest effect of all on the E. faecalis bacterium.

scholarly journals COMPARISON OF APICAL LEAKAGE VALUE BETWEEN EUCALYPTOL AND ORANGE OIL AS GUTTA PERCA SOLVENT

2021 ◽  
Vol 6 (2) ◽  
pp. 201
Author(s):  
Sherli Diana ◽  
Rizki Sri Yuliati ◽  
Bayu Indra Sukmana
Keyword(s):  
Root Canal ◽  
Positive Control ◽  
Root Canal Treatment ◽  
Control Group ◽  
Control Group Design ◽  
Orange Oil ◽  
Carcinogenic Substance ◽  
Gutta Percha ◽  
Significant Difference ◽  
Apical Leakage

Background: The success or failure of root canal treatment is influenced by anatomical factors of the root canal, bacterial infection that causes inadequate hygiene, formation and filling of root canals, iatrogenic factors and several other factors. The average success of root canal treatment is 86-95%, while the failure of canal treatment ranges from 5-14%. Root canal re-treatment can be performed in the event of failure of root canal treatment. The method that can be used is gutta percha solvent. The gutta percha solvents used were Chloroform, Eucalyptol, Orange Oil, and Xylene, but chloroform has been categorized as a carcinogenic substance. Guta percha solvent can cause the intercrystalline distance to be larger, so that it can damage the apical density at obturation. This can create a gap between the gutta percha and the sealer which can cause apical leakage. Objective: To compare the value of apical leakage after root canal re-treatment between gutta percha solvent eucalyptol and orange oil. Method: The research method used is True Experimental with post-test only with control group design. A minimum sample of 8 teeth in a group. Total samples from all groups were 24 teeth. The teeth were stained using methylene blue and measured using stereomycroscopes. Results: the highest mean apical leakage was eucalyptol (4.55±2.59), xylene (2.77±1.94), and the lowest was citrus oil (2.20 ±1.06). The results of One-Way ANOVA test showed that there were no significant differences between orange oil, eucalyptol, and xylene (positive control). Conclusion: Eucalyptol has a higher average apical leakage value compared to orange oil and Xylene after root canal re-treatment as a gutta percha solvent. There was no significant difference between orange oil, Eucalyptol, and Xylene.Keywords: Apical leakage, eucalyptol, orange oil

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