GAG Multivalent Systems to interact with Langerin

2021 ◽  
Vol 28 ◽  
Author(s):  
Javier Rojo ◽  
Pedro M. Nieto ◽  
José Luis de Paz
Keyword(s):  
Extracellular Matrix ◽  
Cell Membrane ◽  
Langerhans Cells ◽  
Pivotal Role ◽  
Monomeric Form ◽  
Recognition Sites ◽  
Interaction Processes ◽  
Carbohydrate Ligands ◽  
The Way

: Langerin is a C-type Lectin expressed at the surface of Langerhans cells, which play a pivotal role in protecting organisms against pathogen infections. To address this aim, Langerin presents at least two recognition sites, one Ca2+-dependent and another one independent, capable of recognizing a variety of carbohydrate ligands. In contrast to other lectins, Langerin recognizes sulfated glycosaminoglycans (GAGs), a family of complex and heterogeneous polysaccharides present in the cell membrane and the extracellular matrix at the interphase generated in the trimeric form of Langerin but absent in the monomeric form. The complexity of these oligosaccharides has impeded the development of well-defined monodisperse structures to study these interaction processes. However, in the last few decades, an improvement of synthetic developments to achieve the preparation of carbohydrate multivalent systems mimicking the GAGs has been described. Despite all these contributions, very few examples are reported where the GAG multivalent structures are used to evaluate the interaction with Langerin. These molecules should pave the way to explore these GAG-Langerin interactions.

Initial characterization of anosmin-1, a putative extracellular matrix protein synthesized by definite neuronal cell populations in the central nervous system

1996 ◽  
Vol 109 (7) ◽  
pp. 1749-1757 ◽  
Author(s):  
N. Soussi-Yanicostas ◽  
J.P. Hardelin ◽  
M.M. Arroyo-Jimenez ◽  
O. Ardouin ◽  
R. Legouis ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Extracellular Matrix ◽  
Nervous System ◽  
Cell Membrane ◽  
Heparan Sulfate ◽  
Matrix Protein ◽  
Neuronal Cell ◽  
Extracellular Matrix Protein ◽  
Cell Populations

The KAL gene is responsible for the X-chromosome linked form of Kallmann's syndrome in humans. Upon transfection of CHO cells with a human KAL cDNA, the corresponding encoded protein, KALc, was produced. This protein is N-glycosylated, secreted in the cell culture medium, and is localized at the cell surface. Several lines of evidence indicate that heparan-sulfate chains of proteoglycan(s) are involved in the binding of KALc to the cell membrane. Polyclonal and monoclonal antibodies to the purified KALc were generated. They allowed us to detect and characterize the protein encoded by the KAL gene in the chicken central nervous system at late stages of embryonic development. This protein is synthesized by definite neuronal cell populations including Purkinje cells in the cerebellum, mitral cells in the olfactory bulbs and several subpopulations in the optic tectum and the striatum. The protein, with an approximate molecular mass of 100 kDa, was named anosmin-1 in reference to the deficiency of the sense of smell which characterizes the human disease. Anosmin-1 is likely to be an extracellular matrix component. Since heparin treatment of cell membrane fractions from cerebellum and tectum resulted in the release of the protein, we suggest that one or several heparan-sulfate proteoglycans are involved in the binding of anosmin-1 to the membranes in vivo.

scholarly journals Harnessing the Topography of 3D Spongy-Like Electrospun Bundled Fibrous Scaffold via a Sharply Inclined Array Collector

Polymers ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1444 ◽  
Author(s):  
Sun Hee Cho ◽  
Jeong In Kim ◽  
Cheol Sang Kim ◽  
Chan Hee Park ◽  
In Gi Kim
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Three Dimensional ◽  
Living System ◽  
Pivotal Role ◽  
Skin Tissue ◽  
Target Tissue ◽  
Fibrous Scaffold ◽  
Electrospun Scaffolds ◽  
Directional Change

To date, many researchers have studied a considerable number of three-dimensional (3D) cotton-like electrospun scaffolds for tissue engineering, including the generation of bone, cartilage, and skin tissue. Although numerous 3D electrospun fibrous matrixes have been successfully developed, additional research is needed to produce 3D patterned and sophisticated structures. The development of 3D fibrous matrixes with patterned and sophisticated structures (FM-PSS) capable of mimicking the extracellular matrix (ECM) is important for advancing tissue engineering. Because modulating nano to microscale features of the 3D fibrous scaffold to control the ambient microenvironment of target tissue cells can play a pivotal role in inducing tissue morphogenesis after transplantation in a living system. To achieve this objective, the 3D FM-PSSs were successfully generated by the electrospinning using a directional change of the sharply inclined array collector. The 3D FM-PSSs overcome the current limitations of conventional electrospun cotton-type 3D matrixes of random fibers.

Changing spaces, changing behaviours: Achaemenid spatial features at the court of Alexander the Great

2018 ◽  
Vol 6 (2) ◽  
pp. 185-214
Author(s):  
Stephen Harrison
Keyword(s):  
Pivotal Role ◽  
Alexander The Great ◽  
Spatial Features ◽  
The Way

AbstractAlexander’s conquest of Persia transformed the way he ruled, with aspects of Achaemenid monarchy becoming prominent. In general, historians have focused on instances of deliberate engagement with Achaemenid practices (e. g. dress, proskynesis, etc.), leading to the impression that this change resulted from conscious imitation. Here, I nuance this view, arguing that the gradual adoption of aspects of Achaemenid royal space played a pivotal role in transforming Alexander’s monarchy. This approach shifts our focus away from Alexander himself, placing his reign in a wider context, while also demonstrating how space can act as a conduit for cultural interchange.

Paul’s Map and Territory: Rethinking the Work of the Apostle in Light of Ancient Cartography

2020 ◽  
Vol 42 (1) ◽  
pp. 90-107
Author(s):  
Eric Smith
Keyword(s):  
Space And Place ◽  
Pivotal Role ◽  
Clear Understanding ◽  
2 Corinthians ◽  
Roman World ◽  
The World ◽  
End Of The World ◽  
Ancient Maps ◽  
The Way

Abstract Paul had a clear understanding of how his calling and his work mapped onto geography. In contexts where he felt that others were encroaching on his territory, as in Galatians and 2 Corinthians, Paul could be very angry and defensive. Likewise, when Paul was writing to people in territories that he did not consider part of his purview, such as in Romans, he was deferential and submissive. In all three cases—in Galatians and 2 Corinthians when Paul was being defensive about his territory, and in Romans when he was being deferential—Paul used a particular word, κλίµα, to designate geography—a word he never used in any other context. This article puts this observation in conversation with ancient mapping, which relied on “process descriptions” of space and place rather than “state descriptions.” That is, ancient cartography privileged the process of movement or travel, and in contrast to most modern mapping, ancient maps didn’t usually make use of any external system of reference. One particular map, the Peutinger Map, helps illustrate this phenomenon. Understanding how ancient maps organized space, we can begin to understand Paul’s notions of territory and the way they determined which places he felt compelled to visit. By knowing something about Paul’s maps and geographies, we can make sense of his language in Romans 15, where territory played a pivotal role in his self-understanding as an apostle and in his trajectory across the Roman world, “from Jerusalem and as far around as Illyricum,” but also onward to Spain and to the end of the world.

The Role of Dystroglycan and Its Ligands in Physiology and Disease

Physiology ◽  
2000 ◽  
Vol 15 (5) ◽  
pp. 255-259 ◽  
Author(s):  
Thomas Meier ◽  
Markus A. Ruegg
Keyword(s):  
Extracellular Matrix ◽  
Cell Membrane ◽  
Basement Membrane ◽  
Embryonic Development ◽  
Bacterial Pathogens ◽  
Membrane Integrity ◽  
Muscle Disease ◽  
Entry Point ◽  
Cell Membrane Integrity

Dystroglycan contributes to the formation of basement membrane during embryonic development and enforces cell membrane integrity by bridging cytoskeleton and components of the extracellular matrix. In several forms of muscle disease, dystroglycan is reduced in abundance. Moreover, human viral and bacterial pathogens use dystroglycan as their cellular entry point.

Optimization of Welding Electrode Coating Composition Based on Simulation of Interaction Processes in Metal-Slag-Gas System

2016 ◽  
Vol 870 ◽  
pp. 593-597
Author(s):  
M.P. Shalimov ◽  
E.B. Votinova
Keyword(s):  
Theoretical Investigation ◽  
Arc Welding ◽  
Electrode Coating ◽  
Interaction Processes ◽  
Manual Arc Welding ◽  
Gas System ◽  
Coating Composition ◽  
Welding Electrode ◽  
The Way

The way of calculation of welding electrode coating composition using the results of experimental and theoretical investigation into the processes of interaction in a metal-slag-gas system under the conditions of manual arc welding is described.

scholarly journals Social Interaction Processes and Personality

2020 ◽  
Author(s):  
Mitja Back
Keyword(s):  
Individual Differences ◽  
Social Interaction ◽  
Personality Traits ◽  
Social Interactions ◽  
Empirical Research ◽  
Social Relationships ◽  
Interaction Processes ◽  
Interaction Partners ◽  
The Way

Social interactions are one of the most relevant contexts of our lives and they are intimately connected to the conceptualization, dynamics, development, and consequences of personality. In this chapter, I will first analyze the way social interactions unfold via interaction states of all interaction partners and describe how people differ in social interaction processes. Following the PERSOC model, I will argue that these individual differences are a key window to understanding the nature of some of the most popular personality traits (e.g., extraversion, dominance, shyness, agreeableness, narcissism), as well as their effects on and development in social relationships. Empirical research on individual differences in interaction state levels, contingencies, and fluctuations is summarized. In closing, I describe a couple of current limitations, and outline perspectives for understanding and assessing personality traits as dynamic social interaction systems.

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