Ameliorating Effects of Lithium on the Perinatal Ethanol-Induced Behavioral and Cognitive Dysfunction and Brain Oxidative Stress in Postnatal Developing Mice Pups

2020 ◽  
Vol 21 (13) ◽  
pp. 1325-1332
Author(s):  
Mohammad Ahmad ◽  
Gasem M. Abu Taweel
Keyword(s):  
Oxidative Stress ◽  
Brain Tissue ◽  
Emotional Dysregulation ◽  
Learning Capability ◽  
Weaning Age ◽  
Pregnant Mice ◽  
Term Basis ◽  
Significant Deficit ◽  
Morphological Indices ◽  
And Oxidative Stress

Background: Developmental ethanol (EtOH) exposure can cause lifelong behavioral hyperactivity, cognitive deficits, emotional dysregulation, and more. However, co-treatment with lithium (Li) on the day of EtOH exposure prevents many of the impairments. Methods: Experimental groups of pregnant mice were exposed to EtOH (20% v/v solution at a dose of 2.5 g/kg) in their drinking water and the animals were treated with Li (15 and 30 mg/kg) through IP injection on gestational days14, 16, 18, and 20, and post-natal days (PD) 3, 5, 7, and 9. All treatments with EtOH and exposure to Li doses to pregnant mice started on gestational day 14 and continued until post-natal day 9 (PD9). The effects on some developing morphological indices, nerve reflexes during weaning age, and various cognitive dysfunctions at adolescent ages and biochemical changes in the brain tissue indices of below-mentioned neurotransmitters and oxidative stress in post-natal developing offspring at adolescent age, were studied. Results: Perinatal exposure to EtOH in pregnant mice resulted in several postnatal developing and morphological indices in the developing male pups during their weaning period, like gain in their body weight, delay in appearance of their body hair fuzz and opening of their eyes, and disruptions in their developing motor reflexes. Discussion: During adolescent age, a significant deficit in their learning capability and cognitive behavior, decline in the neurochemical DA and 5-HT in their brain and some indices of oxidative stress TBARS, GSH, GST, CAT, and SOD was observed. Conclusion: These results indicate that Li ameliorates significantly and dose-dependently EtOH induced developmental toxicities like morphological developments and dysfunctions in cognitive retention and oxidative stress on a long-term basis in brain tissue. However, further detailed studies are required for the clinical use of as an ameliorating agent for perinatal EtOH induced dysfunctions.

Protective Effects of Carvacrol on Brain Tissue Inflammation and Oxidative Stress as well as Learning and Memory in Lipopolysaccharide-Challenged Rats

2019 ◽  
Vol 37 (4) ◽  
pp. 965-976 ◽  
Author(s):  
Zhara Hakimi ◽  
Hossein Salmani ◽  
Narges Marefati ◽  
Zohre Arab ◽  
Zahra Gholamnezhad ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Learning And Memory ◽  
Brain Tissue ◽  
Protective Effects ◽  
Tissue Inflammation ◽  
And Oxidative Stress

scholarly journals Dietary Spray-Dried Porcine Plasma Prevents Cognitive Decline in Senescent Mice and Reduces Neuroinflammation and Oxidative Stress

2019 ◽  
Author(s):  
Alba Garcia-Just ◽  
Lluïsa Miró ◽  
Anna Pérez-Bosque ◽  
Concepció Amat ◽  
Javier Polo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cognitive Decline ◽  
Brain Tissue ◽  
Cognitive Performance ◽  
Dietary Supplementation ◽  
Low Grade ◽  
Anti Inflammatory ◽  
Samp8 Mice ◽  
And Oxidative Stress ◽  
Spray Dried

ABSTRACT Background Aging is characterized by chronic, low-grade inflammation that correlates with cognitive decline. Dietary supplementation with spray-dried porcine plasma (SDP) reduces immune activation in rodent models of inflammation and aging. Objective We investigated whether the anti-inflammatory properties of SDP could ameliorate age-related cognitive deterioration and preserve brain homeostasis in an aging mouse model of senescence. Methods Male senescence-accelerated prone 8 (SAMP8) mice were used. In Experiment 1, cognitive performance (n  = 10–14 mice/group) was analyzed by the novel object recognition test in 2-mo-old mice (2M group) and in mice fed a control diet or a diet supplemented with 8% SDP for 2 (4M-CTL and 4M-SDP groups) and 4 mo (6M-CTL and 6M-SDP groups). In Experiment 2, the permeability of the blood–brain barrier and junctional proteins in brain tissue was assessed, as well as synaptic density, oxidative stress markers, and inflammatory genes and proteins in mice from the 2M, 6M-CTL, and 6M-SDP groups ( n = 5–11). Statistical analyses included one-factor ANOVA followed by Fisher's posthoc test. Results 6M-SDP mice had better cognitive performance than 6M-CTL mice in both short-term (P = 0.024) and long-term (P = 0.017) memory tests. In brain tissue, 6M-SDP mice showed reduced brain capillary permeability (P = 0.034) and increased ZO1 and E-cadherin expression (both P <0.04) compared with 6M-CTL mice. SDP also prevented the NFκB activation observed in 6M-CTL mice (P = 0.002) and reduced Il6 expression and hydrogen peroxide concentration (both P <0.03) observed in 6M-CTL mice. SDP also increased the concentration of IL10 (P = 0.027), an anti-inflammatory cytokine correlated with memory preservation. Conclusions In senescent SAMP8 mice, dietary supplementation with SDP attenuated cognitive decline and prevented changes in brain markers of neuroinflammation and oxidative stress.

scholarly journals Telomere length and oxidative stress variations in a murine model of Alzheimer’s disease progression

2019 ◽  
Author(s):  
Katia Martínez-González ◽  
Azul Islas-Hernández ◽  
José Darío Martínez-Ezquerro ◽  
Federico Bermúdez-Rattoni ◽  
Paola Garcia-delaTorre
Keyword(s):  
Oxidative Stress ◽  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Transgenic Mice ◽  
Telomere Length ◽  
Brain Tissue ◽  
Murine Model ◽  
Blood Cells ◽  
Model Of Alzheimer’S Disease ◽  
And Oxidative Stress

AbstractAlzheimer’s Disease (AD) is the most common cause of dementia and aging is its major risk factor. Changes in telomere length have been associated with aging and some degenerative diseases. Our aim was to explore some of the molecular changes caused by the progression of AD in a transgenic murine model (3xTg-AD; B6; 129-Psen1 <tm1Mpm> Tg (APPSwe, tauP301L) 1Lfa). Telomere length was assessed by qPCR in both brain tissue and peripheral blood cells and compared between three age groups: 5, 9, and 13 months. In addition, a possible effect of oxidative stress on telomere length and AD progression was explored. Shorter telomeres were found in blood cells of older transgenic mice compared to younger and wild type mice but no changes in telomere length in the hippocampus. An increase in oxidative stress with age was found for all strains but no correlation was found between oxidative stress and shorter telomere length for transgenic mice. Telomere length and oxidative stress are affected by AD progression in the 3xTg-AD murine model. Changes in blood cells are more noticeable than changes in brain tissue, suggesting that systemic changes can be detected early in the disease in this murine model.

scholarly journals lncRNA H19 Aggravates Brain Injury in Rats following Experimental Intracerebral Hemorrhage via NF-κB Pathway

2022 ◽  
Vol 2022 ◽  
pp. 1-7
Author(s):  
Shuaidong Mao ◽  
Huan Huang ◽  
Xianzheng Chen
Keyword(s):  
Oxidative Stress ◽  
Brain Injury ◽  
Intracerebral Hemorrhage ◽  
Western Blot ◽  
Water Content ◽  
Brain Tissue ◽  
Sham Group ◽  
Neuron Specific Enolase ◽  
Neurological Function ◽  
And Oxidative Stress

Objective. To explore the effect of long noncoding RNA H19 (lncRNA H19) on brain injury in rats following experimental intracerebral hemorrhage (ICH). Methods. Rat ICH model was established with type IV collagenase. The neurological function scores were evaluated, and the water content in brain tissue was measured. The nerve injury indexes, inflammatory factors, and oxidative stress indexes were also measured. Moreover, the expression of lncRNA H19 was determined by qRT-PCR, and Western blot detected NF-κB pathway-related protein expression. Results. Compared with the sham group, the neurological function scores, the water content in brain tissue, and levels of injury indicators myelin basic protein (MBP), S-100B, and neuron-specific enolase (NSE) in the ICH rats were significantly increased. Meanwhile, the levels of TNF-α, IL-6, IL-1β, ROS, and MDA were significantly increased, but the levels of SOD were significantly decreased. In addition, the expression of lncRNA H19 in the brain tissue in the ICH group was significantly higher than that in the sham group. After further interference with lncRNA H19 expression (sh-H19 group), the levels of all the above indicators were reversed and the neurological damage was improved. Western blot results showed that the expression of NF-κBp65 and IKKβ was significantly higher, and IκBα expression was lower in the perivascular hematoma tissue in the ICH group compared with the sham group. Compared with the sh-NC group, NF-κBp65 and IKKβ expression were significantly lower and IκBα was significantly higher in the sh-H19 group. Conclusion. lncRNA H19 exacerbated brain injury in rats with ICH by promoting neurological impairment, brain edema, and releasing inflammatory responses and oxidative stress. This may be related to the activation of NF-κB signaling pathway.

Neurobehavioral toxic effects of perinatal oral exposure to lithium on the developmental motor reflexes, cognitive dysfunction and brain oxidative stress of mice offspring

2021 ◽  
Vol 1 (1) ◽  
pp. 009-023
Author(s):  
Abdualrahman Saeed Alshehri ◽  
Homood Alharbi ◽  
Mohammad Ahmad
Keyword(s):  
Oxidative Stress ◽  
Cognitive Dysfunction ◽  
Body Weight Gain ◽  
Thiobarbituric Acid ◽  
The Body ◽  
Oral Exposure ◽  
Glutathione S Transferase ◽  
Learning Capability ◽  
Pregnant Mice ◽  
Dose Dependent

In the present study, the perinatal oral exposure of pregnant mice to 15 and 30mg/kg lithium (lithium chloride) in their drinking water resulted in a significant and dose-dependent reduction in postnatal body weight gain, delays in opening of the eyes and appearance in the body hair fuzz, and deficits in the sensory motor reflexes of the mice pups during weaning period (from the day of birth to postnatal day21). At adolescent and adult ages of the male offspring, a significant and dose-dependent deficit was also observed in their learning capability (at PD25), and cognitive behavior (at PD30-36). Furthermore, a significant and dose-dependent disturbance in the levels of neurotransmitters like dopamine (DA) and serotonin (5-HT); non-enzymatic oxidative stress (OS) indices like thiobarbituric acid-reactive substances (TBARS) and total reduced glutathione (GSH); and enzymatic OS indices like glutathione S-transferase (GST), catalase (CAT), and superoxide dismutase (SOD) were observed in the forebrain region of the offspring at post-natal day (PD)7, PD14, PD21, PD30, and PD36. Thus, perinatal lithium exposure can affect the in utero developing fetus, raising the concerns for a potential neurotoxic hazards and a longer lasting cognitive dysfunction. A reduced use of lithium during pregnancy is of crucial importance in preventing lithium -induced neurotoxicity in the offspring.

scholarly journals Camellia sinensis Prevents Perinatal Nicotine-Induced Neurobehavioral Alterations, Tissue Injury, and Oxidative Stress in Male and Female Mice Newborns

2017 ◽  
Vol 2017 ◽  
pp. 1-16 ◽  
Author(s):  
Jamaan S. Ajarem ◽  
Gadh Al-Basher ◽  
Ahmed A. Allam ◽  
Ayman M. Mahmoud
Keyword(s):  
Oxidative Stress ◽  
Camellia Sinensis ◽  
Tissue Injury ◽  
Antioxidant Defenses ◽  
Protective Effects ◽  
Behavioral Alterations ◽  
Male And Female ◽  
Pregnant Mice ◽  
Eye Opening ◽  
And Oxidative Stress

Nicotine exposure during pregnancy induces oxidative stress and leads to behavioral alterations in early childhood and young adulthood. The current study aimed to investigate the possible protective effects of green tea (Camellia sinensis) against perinatal nicotine-induced behavioral alterations and oxidative stress in mice newborns. Pregnant mice received 50 mg/kg C. sinensis on gestational day 1 (PD1) to postnatal day 15 (D15) and were subcutaneously injected with 0.25 mg/kg nicotine from PD12 to D15. Nicotine-exposed newborns showed significant delay in eye opening and hair appearance and declined body weight at birth and at D21. Nicotine induced neuromotor alterations in both male and female newborns evidenced by the suppressed righting, rotating, and cliff avoidance reflexes. Nicotine-exposed newborns exhibited declined memory, learning, and equilibrium capabilities, as well as marked anxiety behavior. C. sinensis significantly improved the physical development, neuromotor maturation, and behavioral performance in nicotine-exposed male and female newborns. In addition, C. sinensis prevented nicotine-induced tissue injury and lipid peroxidation and enhanced antioxidant defenses in the cerebellum and medulla oblongata of male and female newborns. In conclusion, this study shows that C. sinensis confers protective effects against perinatal nicotine-induced neurobehavioral alterations, tissue injury, and oxidative stress in mice newborns.

scholarly journals Brain Tissue Hypoxia and Oxidative Stress Induced by Obstructive Apneas is Different in Young and Aged Rats

SLEEP ◽  
2014 ◽  
Vol 37 (7) ◽  
pp. 1249-1256 ◽  
Author(s):  
Mireia Dalmases ◽  
Marta Torres ◽  
Leonardo Márquez-Kisinousky ◽  
Isaac Almendros ◽  
Anna M. Planas ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Brain Tissue ◽  
Tissue Hypoxia ◽  
Aged Rats ◽  
And Oxidative Stress
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