Proteolytic and lipolytic potential of Pseudomonas spp. from goat and bovine raw milk

ABSTRACT: Pseudomonas, the main genus of gram-negative microorganisms isolated from milk, is psychrotrophic, biofilm-forming, and thermo-resistant deteriorating enzyme producers. The aim of this study was to quantify Pseudomonas spp. in goat’s and cow’s milk produced in the Paraná state, Brazil, to evaluate the deteriorating activity of the isolates at mesophilic and psychrotrophic conditions and to identify, at the species level, the isolates with alkaline metalloprotease (aprX gene) production potential. Microbiological, biochemical and molecular methods were used for isolating, confirming and identifying of isolates. The mean counts were 1.6 (±6.3)x104 and 0.89(±3)x102 CFU/mL for goat and bovine milk samples, respectively, immediately after milking. Of the Pseudomonas colonies isolated from goat milk (n=60), 91.7% showed proteolytic potential when incubated at 35°C/48 h and 80% at 7°C/10 days, and lipolytic potential was observed in 95% of the isolates incubated in mesophilic and 78.3% at refrigeration conditions. From the isolates of bovine milk (n=20), 35% showed proteolytic activity only when incubated at 35°C/48 h, and lipolytic potential was observed in 25% of the isolates incubated at 7°C/10d and 35°C/48h. It was observed that 83.3% and 25% of the isolates genetically confirmed as Pseudomonas spp. of goat and bovine milk showed the potential for alkaline metalloprotease production, with the species P. azotoformans, P. koreensis, P. gessardii, P. monteilii and P. lurida being the most frequent in goat milk and P. aeruginosa the only species identified in cow milk.

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Milk Iodine Content in Slovakia

Acta Veterinaria Brno ◽

10.2754/avb200877040533 ◽

2008 ◽

Vol 77 (4) ◽

pp. 533-538 ◽

Cited By ~ 11

Author(s):

I. Paulíková ◽

H. Seidel ◽

O. Nagy ◽

G. Kováč

Keyword(s):

Dairy Cows ◽

Iodine Deficiency ◽

Bovine Milk ◽

Goat Milk ◽

Iodine Content ◽

Raw Milk ◽

Feeding Period ◽

Seasonal Differences ◽

Iodine Status ◽

Milk Samples

The aim of this work was to map actual iodine status and its seasonal differences in raw milk of dairy cows, sheep, and goats in various regions of Slovakia. Iodine concentrations were determined in 457 samples of raw milk from dairy cows, 78 samples of sheep, and 16 samples of goat milk collected in various regions of Slovakia from 2002 to 2007. Among all the 457 samples of bovine milk, iodine content below 50 μg l-1 was recorded in 114 samples (24.94%); 294 samples (64.33%) ranged between 50 and 200 μg l-1; 19 samples (4.16%) from 200 to 500 μg l-1; 17 samples (3.72%) between 500 and 1 000 μg l-1, and 13 samples (2.85%) showed iodine concentrations over 1 000 μg l-1. regional concentrations showed the highest values in the Western, then Middle and Eastern Slovakia, and the lowest values in Northern Slovakia (p < 0.05, p < 0.01). In sheep and goat milk samples, we found iodine concentrations below 80 μg l-1 in 49 sheep (62.8%) and in 6 goats below 60 μg l-1 (37.5%), which are indicative of iodine deficiency. When comparing seasonal differences, sheep and goat milk had higher iodine content during the winter feeding period, however, in dairy cows we recorded the opposite ratio. Except for goat milk (p < 0.01) the seasonal differences were not significant.

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Snapshot of Cyprus Raw Goat Milk Bacterial Diversity via 16S rDNA High-Throughput Sequencing; Impact of Cold Storage Conditions

Fermentation ◽

10.3390/fermentation6040100 ◽

2020 ◽

Vol 6 (4) ◽

pp. 100

Author(s):

Eleni Kamilari ◽

Dimitrios A. Anagnostopoulos ◽

Photis Papademas ◽

Marina Efthymiou ◽

Svitlana Tretiak ◽

...

Keyword(s):

Bacterial Diversity ◽

High Throughput ◽

Cold Storage ◽

High Throughput Sequencing ◽

Goat Milk ◽

Raw Milk ◽

Storage Conditions ◽

Gram Negative ◽

Milk Samples ◽

The Impact

In general, it is a common practice among dairy producers to store the milk in the refrigerator directly after milking, in order to preserve it and prevent the development of spoilage microbes. However, the impact of keeping the milk in the refrigerator overnight on milk microbial diversity has been poorly investigated. This study aimed to provide a snapshot of the bacterial composition of goat milk after direct storage at −80 °C and after being kept overnight at 4 °C and then in storage at −80 °, using high-throughput sequencing (HTS). Goat milk samples from four different farms were analyzed, to reveal that milk bacterial diversity differed between the two different storage conditions. Goat milk directly stored at −80 °C was characterized by the presence of the Gram-negative contaminants Pseudomonas and Acinetobacter, in addition to the genera Corynebacterium, Chryseobacterium, Bacteroides and Clostridium. Milk samples that were kept overnight at 4 °C were characterized by a reduction in their bacterial biodiversity and the predominance of the Gram-negative, aerobic Phyllobacterium. Overall, HTS methodologies provide an in-depth identification and characterization of the goat raw milk microbiome. Further, they offer a better understanding of the contribution of cold storage conditions to milk microbiota formation. This study may assist dairy producers in improving raw milk and raw milk cheeses quality and guaranteeing consumers’ safety.

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Evaluation of the presence of Aflatoxin-M1 in milk samples sold in the region of Campinas-SP

European Journal of Public Health ◽

10.1093/eurpub/ckaa166.246 ◽

2020 ◽

Vol 30 (Supplement_5) ◽

Author(s):

A C de Souza Zocante ◽

S J de Souza ◽

V F de Souza Rodrigues ◽

M L Caminada ◽

L O Palaria Joaquim

Keyword(s):

Public Health ◽

National Health ◽

Bovine Milk ◽

Goat Milk ◽

Raw Milk ◽

Health Surveillance ◽

Aflatoxin M1 ◽

Public Health Issue ◽

Milk Samples ◽

Hydroxylated Metabolite

Abstract Milk and its derivatives are fundamental foods in the human diet. However, milk is highly susceptible to microbial contamination, among which is Aflatoxin M1 (AFM1), presenting a risk to public health. Aflatoxin M1 is the main hydroxylated metabolite of aflatoxin B1 present in the milk of animals that have ingested feed contaminated with B1. These toxins have carcinogenic and genotoxic potential and similar toxicities. According to Resolution RDC 07/2011, published by the National Health Surveillance Agency (ANVISA), the level of tolerance for AFM1 is 0.5 µg / Kg in Brazil. The present study aimed to evaluate qualitatively and quantitatively Aflatoxin M1 in raw milk samples, being 2 samples of bovine milk and one sample of goat milk. The raw, bovine and goat milk samples were purchased from local stores and were kept at approximately 5 ºC for 8 hours and subsequently frozen at 6 ºC for conservation purposes. The tests were carried out at the premises of Hexis Científica, Av. Antonieta Piva Barranqueiros, 385 - Distrito Industial - Jundiaí-SP, which provided training and use of the laboratory. The Scientific Charm Kits, Test SLAFMQ-EZ-20K were used. It is a quantitative test that uses ROSA immunoreceptors (Rapid Assay Step One) lateral flow technology. The milk sample interacts with colored spheres and the intensity of the color in the test area and is read as ppt (parts per trillion) by the reader. According to the analyzes, all the samples studied presented Aflatoxin M1, with a concentration of 2,507 ppb; 2,410 ppb; 2,627 ppb, respectively for bovine and caprine milk samples, which characterizes a concentration five times above the allowed by the current legislation, according to RDC 07/2011, published by the National Health Surveillance Agency (ANVISA). Key messages According to studies, most of the negative results obtained on the incidence of aflatoxin M1 can be attributed to the low sensitivity of the analytical methods employed. The presence of Aflatoxins M1, represents a public health issue, since milk is among the products most consumed by man and, constituting the basis of infant feeding.

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Bacteriological quality and safety of raw cow milk in Madurai (South India)

Bangladesh Journal of Scientific and Industrial Research ◽

10.3329/bjsir.v48i2.15741 ◽

2013 ◽

Vol 48 (2) ◽

pp. 109-114 ◽

Cited By ~ 2

Author(s):

S Lingathurai ◽

P Vellathurai

Keyword(s):

Microbiological Quality ◽

Raw Milk ◽

Cow Milk ◽

Coliform Bacteria ◽

Plate Count ◽

Quality And Safety ◽

E Coli ◽

Milk Samples ◽

Total Plate Count ◽

The Mean

The microbiological quality and safety of raw milk from 60 dairy farms in Madurai were determined. Milk samples were collected at 60 centers from four regions, namely northern, eastern, western and southern (NEWS) according to stratified random sampling design. Samples were analyzed for Total plate count (TPC), psychrotrophs, thermophiles, Staphylococcus aureus, coliform, Escherichia coli 0157: H7 and Salmonella. The mean counts per ml for TPC, psychrotrophs and thermophiles were 12.5x106, 5x103 and 6.85x103 respectively. From the 60 milk samples tested, coliform bacteria contaminated approximately 90% and 70% were E. coli positive, with mean counts ranged from 103 to 104 cfu ml-1. S. aureus was isolated from more than 61.7% of the samples and the mean count per ml was 6.2x103. Meanwhile, E. coli 0157: H7 was also detected in 39 (65%) samples. However, Salmonella was only detected in 8 (13.3%) of the samples with the southern region having the highest frequency of isolation. Bangladesh J. Sci. Ind. Res. 48(2), 109-114, 2013 DOI: http://dx.doi.org/10.3329/bjsir.v48i2.15741

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Coagulation Traits of Sheep and Goat Milk

Animals ◽

10.3390/ani9080540 ◽

2019 ◽

Vol 9 (8) ◽

pp. 540 ◽

Cited By ~ 7

Author(s):

Michele Pazzola

Keyword(s):

Bovine Milk ◽

Goat Milk ◽

Raw Milk ◽

Cow Milk ◽

Coagulation Time ◽

Sheep Milk ◽

Rennet Coagulation ◽

Raw Milk Cheese ◽

Predictive Formulas

Milk production from sheep and goat species is continuously growing worldwide, and its main use is for cheesemaking. Given that the final quality of cheese is linked to the traits of raw milk cheese yield at dairy plants, it is often calculated by using predictive formulas based on fat and protein content. Predictive formulas have been studied for bovine milk and are very effective but not appropriate for sheep and goat milk. Several methods, which simulate the actual coagulation processes, are available at the laboratories. This article reviews the available literature about rennet coagulation and cheese yield traits from sheep and goat milk and the methods used at the laboratory level. In general, if compared to cow milk, sheep and goat milk are characterized by shorter rennet coagulation times and a very limited amount of non-coagulating samples. Curd firmness of sheep milk is almost independent from the rennet coagulation time, and some coagulation traits can be predicted by infrared spectra. In addition, coagulation traits are characterized by appropriate values of heritability to be considered in selective breeding plans. With regard to goat milk, rennet coagulation time and cheese yield are strongly influenced by the breed effect.

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Keeping Quality and Flavor, and Microorganisms, Proteases and Lipases in Raw Cow and Goat Milk at Collection and After Storage

Journal of Food Protection ◽

10.4315/0362-028x-46.10.873 ◽

1983 ◽

Vol 46 (10) ◽

pp. 873-877 ◽

Cited By ~ 1

Author(s):

LESTER HANKIN ◽

DONALD SHIELDS

Keyword(s):

Protein Degradation ◽

Goat Milk ◽

Raw Milk ◽

Cow Milk ◽

Coliform Bacteria ◽

Microbial Count ◽

Retail Sale ◽

Milk Samples ◽

Significant Difference ◽

Keeping Quality

Raw cow and goat milks for retail sale in Connecticut were examined at collection and after storage at 4.4 and 7.2°C for 7 d for keeping quality, flavor, microorganisms, protein degradation on storage, and protease and lipase activity. Some milks were bottled at the farm, others were placed in containers supplied by the customer. Goat milk retained a satisfactory flavor significantly longer than cow milk. There was no correlation of keeping quality with any microbial count made at collection except for number of coliform bacteria. Significant differences were found in enzyme activity and protein degradation between cow and goat milk. No significant difference was found between milk bottled at the farm and that collected in sterile containers. Over 82% of raw milk samples met the 30,000 per ml state standard for total aerobic count (SPC) and 72% met the coliform standard of 50 per ml.

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Evaluation of the presence of antibiotics in milk samples sold in the region of Campinas - SP

European Journal of Public Health ◽

10.1093/eurpub/ckaa166.257 ◽

2020 ◽

Vol 30 (Supplement_5) ◽

Author(s):

S M Lenzi Caminada ◽

A C de Souza Zocante ◽

S J de Souza ◽

V F de Souza Rodrigues ◽

I O Palaria Joaquim ◽

...

Keyword(s):

Antimicrobial Agents ◽

Bovine Milk ◽

Goat Milk ◽

Dairy Herd ◽

Raw Milk ◽

Public Health Issue ◽

Growth Promoters ◽

Production Chain ◽

Milk Samples ◽

Consumer Safety

Abstract Milk is considered one of the most complete sources of health nutrients and is quite susceptible to contamination by antimicrobial residues due to the indiscriminate use of veterinary drugs to treat dairy cattle, mainly in the treatment of mastitis in cows. Antibiotic residues are considered chemical contaminants, arising from the treatment of pathologies of the dairy herd without adequate control of the disposal period provided by each medication. Milk may also contain residues of other substances from agricultural products and / or inputs and environmental residues, such as: antiparasitic agents, growth promoters, milking hygiene products, heavy metals and mycotoxins. The present study aimed to qualitatively evaluate the presence of antibiotics in raw milk samples, being 2 samples of bovine milk and one sample of goat milk. The samples of raw, bovine and goat milk were purchased from local stores and were kept at approximately 5 ºC for 8 hours and subsequently frozen at - 6 ºC for conservation purposes. The tests were carried out at the premises of Hexis Científica, Av. Antonieta Piva Barranqueiros, 385 - Distrito Industial - Jundiaí-SP, which provided training and use of the laboratory. Kit Charm QUAD-1, Test LF-BRPAC-100K, interpretation of the EZ System test (HX0066-00205) which detects the presence of Beta-lactams, Quimolones, Sulfa drugs, Tetraciclynes, Macrolides, Gentamicin, Aminoglycosides and Amphenicols, covering the drugs required in the National Contaminant Waste Plan-PNCRC. According to the analyzes, the samples studied did not show residues of antibiotics, which demonstrates the responsibility of producers with current legislation and consumers. Key messages As for the public health issue, different types of risks are associated with the presence of antimicrobials in milk, such as microbiological, immunopathological and toxic-pharmacological. The carrying out of information actions, in the production chain, regarding the risks arising from the ingestion of food contaminated by antimicrobial agents is essential for consumer safety.

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A2 Bovine Milk and Caprine Milk as a Means of Remedy for Milk Protein Allergy

Dairy ◽

10.3390/dairy2020017 ◽

2021 ◽

Vol 2 (2) ◽

pp. 191-201

Author(s):

Young W. Park ◽

George F. W. Haenlein

Keyword(s):

Milk Protein ◽

Bovine Milk ◽

Goat Milk ◽

Food Sources ◽

Cow Milk ◽

Cow’S Milk ◽

Dairy Goat ◽

Cow's Milk ◽

Caprine Milk ◽

Low Levels

A new type of cow’s milk, called A2 milk, has appeared in the dairy aisles of supermarkets in recent years. Cows’ milk generally contains two major types of beta-casein as A1 and A2 types, although there are 13 genetic variants of β-casein: A1, A2, A3, A4, B, C, D, E, F, H1, H2, I and G. Studies have shown that A1 β-casein may be harmful, and A2 β-casein is a safer choice for human health especially in infant nutrition and health. The A2 cow milk is reportedly easier to digest and better absorb than A1 or other types of milk. The structure of A2 cow’s milk protein is more comparable to human breast milk, as well as milk from goats, sheep and buffalo. Digestion of A1 type milk produces a peptide called β-casomorphin-7 (BCM-7), which is implicated with adverse gastrointestinal effects on milk consumption. In addition, bovine milk contains predominantly αs1-casein and low levels or even absent in αs2-casein, whereby caprine milk has been recommended as an ideal substitute for patients suffering from allergies against cow milk protein or other food sources. Since goat milk contains relatively low levels of αs1-casein or negligible its content, and αs2-casein levels are high in the milk of most dairy goat breeds, it is logical to assume that children with a high milk sensitivity to αs1-casein should tolerate goat milk well. Cow milk protein allergy (CMPA) is considered a common milk digestive and metabolic disorder or allergic disease with various levels of prevalence from 2.5% in children during the first 3 years of life to 12–30% in infants less than 3 months old, and it can go up to even as high as 20% in some countries. CMPA is an IgE-mediated allergy where the body starts to produce IgE antibodies against certain protein (allergens) such as A1 milk and αs1-casein in bovine milk. Studies have shown that ingestion of β-casein A1 milk can cause ischemic heart disease, type-1 diabetes, arteriosclerosis, sudden infant death syndrome, autism, schizophrenia, etc. The knowledge of bovine A2 milk and caprine αs2-casein has been utilized to rescue CMPA patients and other potential disease problems. This knowledge has been genetically applied to milk production in cows or goats or even whole herds of the two species. This practice has happened in California and Ohio, as well as in New Zealand, where this A2 cow milk has been now advanced commercially. In the USA, there have been even promotions of bulls, whose daughters have been tested homozygous for the A2 β-casein protein.

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Growth and enterotoxin production of Bacillus cereus in cow, goat, and sheep milk

Acta Veterinaria Brno ◽

10.2754/avb201483s10s3 ◽

2014 ◽

Vol 83 (10) ◽

pp. S3-S8 ◽

Cited By ~ 5

Author(s):

Lenka Necidová ◽

Šárka Bursová ◽

Alena Skočková ◽

Bohdana Janštová ◽

Pavla Prachařová ◽

...

Keyword(s):

Bacillus Cereus ◽

Storage Temperature ◽

Goat Milk ◽

Storage Conditions ◽

Cow Milk ◽

Enzyme Linked Immunosorbent Assay ◽

Plate Method ◽

Milk Samples ◽

Sheep Milk ◽

Enterotoxin Production

The aim of this study was to compare Bacillus cereus growth rates and diarrhoeal enterotoxin production in raw and pasteurized goat, sheep, and cow milk in terms of storage conditions. Milk samples were inoculated with B. cereus (CCM 2010), which produces diarrhoeal enterotoxins. Enterotoxin production was tested by ELISA (Enzyme-Linked Immunosorbent Assay), and the count of B. cereus was determined by the plate method. With raw cow milk, B. cereus growth and enterotoxin production can be completely suppressed; in raw goat and sheep milk, enterotoxin was produced at 22 °C. In pasteurized cow, goat, and sheep milk, the B. cereus count increased under all storage conditions, with more rapid growth being observed at 15 °C (sheep milk) and 22 °C (cow and goat milk). Enterotoxin presence was detected at 15 °C and 22 °C, and with pasteurized cow milk also at 8 °C. Our model experiments have determined that B. cereus multiplication and subsequent enterotoxin production depend on storage temperature and milk type.

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Occurrence of aflatoxin M1 in bovine milk and associated risk factors among dairy farms of Punjab, India

World Mycotoxin Journal ◽

10.3920/wmj2021.2697 ◽

2021 ◽

pp. 1-10

Author(s):

H. Thukral ◽

P. Dhaka ◽

J. Singh Bedi ◽

R. Singh Aulakh

Keyword(s):

Risk Factors ◽

European Union ◽

Bovine Milk ◽

Dairy Farms ◽

Buffalo Milk ◽

Cow Milk ◽

Milk Samples ◽

Significant Difference ◽

Associated Risk Factors ◽

Mean Concentration

Aflatoxin M1 (AFM1) contamination in milk and milk products may pose a major public health concern. The present cross-sectional study was aimed to estimate the prevalence of AFM1 in bovine milk across all districts of Punjab, India and to identify the associated animal and farm level risk factors. A total of 402 milk samples (266 cow milk and 136 buffalo milk) were analysed using commercial ELISA and representative samples were confirmed using HPLC-FLD. The results revealed that 56.2 and 13.4% of the milk samples exceeded the maximum levels of the European Union, i.e. 0.05 μg/l and Food Safety and Standards Authority of India (FSSAI), i.e. 0.5 μg/l for AFM1 in milk, respectively. On analysis of species variation, buffalo milk (prevalence: 56.6%; mean concentration: 0.42±0.9 μg/l) was found to have higher AFM1 levels than cow milk (prevalence: 56.0%; mean concentration: 0.19±0.3 μg/l), with statistically significant difference between mean concentrations (P<0.01) and non-significant difference between AFM1 prevalence (P=0.91). Furthermore, milk from commercial dairy farms (prevalence: 64.7%; mean concentration: 0.34±0.65 μg/l) was found to be more contaminated than from household dairy establishments (prevalence: 47.8%; mean concentration: 0.19±0.65 μg/l). The risk factors ‘above average milk yield/day’ (odds ratio (OR): 2.4) and ‘poor animal hygiene’ (OR: 1.9) were identified at animal level, and ‘intensive dairy farming’ (OR: 3.1) and ‘animal feed without aflatoxin binder’ (OR: 4.7) as farm level risk factors for AFM1 excretion above maximum levels of European Union in milk. Among cow breeds, the milk from ‘non-descript’ breed (OR: 11.5) was found to be most contaminated with AFM1 and the least from Jersey breed (OR: 1.0). The present study highlighted the presence of AFM1 in milk samples; therefore, regular monitoring of AFM1 in milk is required so that high risk regions and associated risk factors can be addressed appropriately.

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