Evaluation of Cytotoxic Activity of Lavandula stoechas Aerial Parts Fractions against HepG2 Cell Lines

2020 ◽  
Vol 16 (9) ◽  
pp. 1281-1289
Author(s):  
Mohd. A. Siddiqui ◽  
Hefazat H. Siddiqui ◽  
Anuradha Mishra ◽  
Afreen Usmani
Keyword(s):  
Ethyl Acetate ◽  
Hepg2 Cell ◽  
Mtt Assay ◽  
Cytotoxic Effect ◽  
Morphological Changes ◽  
Assay Method ◽  
Lavandula Stoechas ◽  
Aerial Parts ◽  
Hepg2 Cell Lines ◽  
First Time

Background: Lavandula stoechas is the main member of Labiatae (Lamiaceae) family. People use this medicinal plant in the treatment of several diseases around the world. The purpose of this work was to evaluate the cytotoxic effect of different fractions like ethanol, ethyl acetate and n-hexane from methanol extract of Lavandula stoechas aerial parts on HepG2 cell line and analysis of chemical composition of the most active fraction of the Lavandula stoechas aerial parts. Methods: Methanolic extract was prepared by cold maceration. Fractions from extract were prepared in solvents of increasing polarity i.e. n-hexane, ethyl acetate and ethanol. In order to evaluate the cytotoxic effect of Lavandula stoechas, MTT assay method was used. For this purpose, HepG2 cells line were treated with the above mentioned Lavandula stoechas aerial parts fractions with different concentrations 10, 25, 50, 100, 250, 500 and 1000 μg/ml. The cells without treatment were served as a control. Results: The result of this study showed that ethanolic fraction showed major reduction in % cell survival compared to other fractions. These results showed that Lavandula stoechas Ethanolic fraction was highly inhibitory. Further, phyto chemical analysis of ethanolic fraction was carried out using GC-MS technique and the reports showed Lupeol, Phytol, α-Cadinol, Lup-20(29)-en-3-one, hydrocoumarin, Lup-20(29)-en-3-one. Other compounds included fatty acids and their esters. Some of these compounds are being first time reported here from this plant. Conclusion: MTT assay showed morphological changes, which clearly support Lavandula stoecha ethanolic fraction possesses anticancer effect which may be due to the presence of phytosterols.

scholarly journals Six New Coumarin Glycosides from the Aerial Parts of Gendarussa vulgaris

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1456 ◽  
Author(s):  
Yanjun Sun ◽  
Meiling Gao ◽  
Haojie Chen ◽  
Ruijie Han ◽  
Hui Chen ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Hepg2 Cell ◽  
Mtt Assay ◽  
Spectroscopic Data ◽  
Hplc Analysis ◽  
Cytotoxic Activities ◽  
Aerial Parts ◽  
Structure Activity ◽  
Hepg2 Cell Lines ◽  
Mcf 7

Six new coumarin glycosides, genglycoside A–F (1–6), were isolated from the aerial parts of Gendarussa vulgaris, along with ten known analogues (7–16). Their structures were unambiguously established on the basis of extensive spectroscopic data and HPLC analysis. The cytotoxic activities of all isolated compounds were evaluated by MTT assay. Compound 12 showed the most potent cytotoxicity in Eca-109, MCF-7, and HepG2 cell lines. By the preliminary structure–activity relationships, it was firstly discovered that the glycosylation or esterification at 7,8-dihydroxy or 7-hydroxy drastically reduced the cytotoxic activity of the parent coumarin.

scholarly journals Extraction, Structural Characterization, and Anti-Hepatocellular Carcinoma Activity of Polysaccharides From Panax ginseng Meyer

2021 ◽  
Vol 11 ◽  
Author(s):  
Hui Jia ◽  
Bin Zhao ◽  
Fangfang Zhang ◽  
Ramesh Kumar Santhanam ◽  
Xinying Wang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepg2 Cells ◽  
Cytotoxic Effect ◽  
Structural Characteristics ◽  
Gel Permeation Chromatography ◽  
Salting Out ◽  
Gel Permeation ◽  
Acidic Polysaccharides ◽  
Hepg2 Cell Lines ◽  
First Time

Polysaccharides are the main active ingredients of ginseng. To extract the most effective polysaccharides against hepatocellular carcinoma (HCC), we isolated and characterized the polysaccharides from the mountain cultivated ginseng (MCG) and compared their composition and cytotoxic effect with cultivated ginseng (CG) polysaccharide against HepG2 cell lines for the first time. MCG polysaccharides and CG polysaccharides were fractionated into two fractions such as MTPS-1, MTPS-2 and CTPS-1, CTPS-2 by salting out, respectively. Compared to CG, MCG possessed appreciable cytotoxic effect against HepG2 cells among that MTPS-1 possess fortified effect. Then, MTPS-1 was selected for further isolation process and seven acidic polysaccharides (MCGP-1–MCGP-7) were obtained using ethanol precipitation, ion-exchange, and gel permeation chromatography techniques. Structural characteristics of the polysaccharides (MCGP-1–MCGP-7) were done by adapting methylation/GC-MS and NMR analysis. Overall, MCGP-3 polysaccharide was found to possess significant cytotoxic effect against HepG2 cells with the IC50 value.

scholarly journals Sixteen New Prenylated Flavonoids from the Fruit of Sinopodophyllum hexandrum

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3196 ◽  
Author(s):  
Yanjun Sun ◽  
Haojie Chen ◽  
Junmin Wang ◽  
Meiling Gao ◽  
Chen Zhao ◽  
...  
Keyword(s):  
Hepg2 Cell ◽  
Mtt Assay ◽  
13C Nmr ◽  
Spectroscopic Data ◽  
Cytotoxic Activities ◽  
Prenylated Flavonoids ◽  
Ic50 Values ◽  
Sinopodophyllum Hexandrum ◽  
Hepg2 Cell Lines ◽  
Mcf 7

Sixteen new prenylated flavonoids, sinoflavonoids P–Z (1–11) and sinoflavonoids NA–NE (12–16), were isolated from the fruit of Sinopodophyllum hexandrum, along with eight known analogues (17–24). Their structures were elucidated on the basis of extensive spectroscopic data (HR-ESI-MS, 1H-NMR, 13C-NMR, HSQC, HMBC). The cytotoxic activities of compounds 1–18, 20, and 22 were evaluated by MTT assay. Compound 6 showed the most potent cytotoxicity in MCF-7, and HepG2 cell lines, with IC50 values of 6.25 and 3.83 μM, respectively.

Cytotoxic Evaluation of Daphne pontica L. Aerial Part Extracts on Three Cancerous Cell Lines by MTT Assay

2019 ◽  
Author(s):  
Behshad Eskandari ◽  
Maliheh Safavi ◽  
Seyede Nargess Sadati Lamardi ◽  
Mahdi Vazirian
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Mtt Assay ◽  
Cytotoxic Effect ◽  
Ethyl Acetate Fraction ◽  
Chemotherapeutic Agents ◽  
Breast Cancer Cell Lines ◽  
Total Extract ◽  
Cancerous Cell

Nowadays, cancer is the second prevalent cause of mortality after cardiovascular diseases in developed and the third one in developing countries. Adverse effects of chemotherapeutic agents bring the necessity of investigating about new medications with fewer side effects. Daphne L. genus is one of the natural sources with valuable reported anticancer effects. This study aimed to assess the cytotoxic effect of some extracts from the aerial parts of Daphne pontica collected from North of Iran on cancer cell lines.Extraction of the plant material was performed by maceration (3×72 h) of 200 g of sample with petroleum ether, chloroform, ethyl acetate, and methanol, respectively. The total extract was also obtained by maceration of the sample with 80% ethanol. Different concentrations of the dried extracts were prepared to assess their cytotoxic effect by 24 h incubation of cell lines with different extracts and then MTT (dimethyl thiazolyl diphenyl tetrazolium) assay on three cancerous cell lines (MDA-MB-231, MCF-7 and T47D), performed in triplicate. IC50 was then estimated from curves constructed by plotting cell survival (%) versus sample concentration (µg/ml). Results indicated that ethyl acetate fraction of D. pontica had the most potent cytotoxic effect in MTT assay with IC50 = 977.46 µg/ml; while other fractions were weaker in toxicity (IC50>1000 µg/ml). By comparing to potent cytotoxic effects of other Daphne species, it seems that the cytotoxic properties of D. pontica is different from other species of this genus since according to this study, no significant antineoplastic properties against the three breast cancer cell lines were determined. Further studies on other pharmacological activities of this plant are recommended.

scholarly journals Cytotoxic Test of Extract and Fraction of Dendrophtoe Pentandra (L) Miq on T47D Cell

2019 ◽  
Vol 22 (3) ◽  
pp. 73-78
Author(s):  
Masayu Farah Diba ◽  
Salni Salni ◽  
Subandrate Subandrate
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Effect ◽  
Linear Equations ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
T47d Cell ◽  
Assay Method ◽  
Cytotoxic Effects ◽  
Ic50 Value ◽  
Ethanol Fraction

Indonesia has a variety of plants that have the potential for medicine. One of the plants used by the community as a drug with anticancer effects is Dendrophtoe petandra (L.) Miq. This study aims to determine the cytotoxic effect of ethanol extract, ethyl acetate fraction and water ethanol fraction of Dendrophtoe petandra (L.) Miq on T47D breast cancer cells. The cytotoxic effects of ethanol extract, ethyl acetate fraction and water ethanol fraction were carried out by MTT assay method using series concentration. Cytotoxic effects were assessed by calculating IC50 values using linear equations. Phytochemical test showed that Dendrophtoe petandra (L.) Miq contained saponins, terpenoids, flavonoids and tannins. The IC50 value of the ethanol extract, ethyl acetate fraction and water ethanol fraction were 417.506 μg/mL, 233.617 μg/mL, and 2748.357 μg/mL, respectively. The smaller the IC50 value means that the compound is more active. Water ethanol fraction of Dendrophtoe petandra (L.) Miq does not have a cytotoxic effect, whereas ethanol extract and ethyl acetate fraction of Dendrophtoe petandra (L.) Miq have cytotoxic effects in the medium strength category. The content of flavonoids and saponins in ethanol extract and ethyl acetate fraction of Dendrophtoe petandra (L.) Miq is thought to play role in causing T47D cell death. Cytotoxic effects of ethyl acetate fraction are stronger than ethanol extracts.

scholarly journals Antiproliferative effect of extracts and fractions of the root of Terminalia avicennioides (Combretaceae) Guill and Perr. on HepG2 and Vero cell lines

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Hadiza Aliyu-Amoo ◽  
Hamza Ibrahim Isa ◽  
Emmanuel Mfotie Njoya ◽  
Lyndy Joy McGaw
Keyword(s):  
Medicinal Plant ◽  
Cell Viability ◽  
Vero Cell ◽  
Hepg2 Cell ◽  
Cell Lines ◽  
Cytotoxic Effect ◽  
Vero Cells ◽  
Antiproliferative Effect ◽  
Vero Cell Lines ◽  
Hepg2 Cell Lines

Abstract Background Terminalia avicennioides Guill and Perr (Combretaceae) is an important West African medicinal plant. The plant is used locally against microbes and parasites in both humans and animals and studies have demonstrated its cytotoxicity potential. Thus, this study was carried out to test the cytotoxic effect of the extracts and fractions of the root of the medicinal plant Terminalia avicennioides Guill and Perr (Combretaceae) in two different cell lines. Methods Methanol, ethanol, 30 % ethanol, hot water and cold water extracts and ethylacetate, hexane, chloroform, butanol and residual water fractions, were evaluated at 1000, 750, 500, 250, 100 and 50 µg/mL concentrations, with doxorubicin as positive control. The cells were incubated with the extracts for 48 h at 37 °C in a 5 % CO2 humidified incubator. The inhibition of cell viability, determined with the methyl blue thiazole tetrazolium bromide (MTT) assay, was used to assess the anti-proliferative effect of the extracts, in normal Vero Monkey kidney and human liver cancer (HepG2) cell lines. Results There was a concentration-dependent inhibition of cell viability in both the HepG2 and Vero cell lines. For HepG2 cells, antiproliferative effect was highest for the hexane fraction (viability ranged from 19.63 ± 1.10 % to 70.30 ± 1.78 % for 1000 and 50 µg/mL, respectively. For Vero cells, the highest antiproliferative effect, at 1000 µg/mL, was with hexane fraction (cell viability 21.37 ± 3.50 %), while at 50 µg/mL the chloroform fraction demonstrated the highest effect (viability of 86.10 ± 1.95 %). Conclusions The extracts and fractions from the root of Terminalia avicennioides have antiproliferative effect on the Vero and HepG2 cell lines tested. However, the extracts and fractions were not more toxic to the HepG2 than to the Vero cells. The cytotoxic effect of stem-bark and leaf extracts could be evaluated in the future to determine its anticancer potential.

scholarly journals Synthesis and Characterization of Mangiferin Loaded N,O-CMC Nanoparticles and its Cytotoxic Effect on Osteosarcoma MG-63 Cells

2020 ◽  
Vol 11 (2) ◽  
pp. 2136-2145
Author(s):  
Puteri Zarith Sofea Yusri ◽  
Nurin Fatini Ghazali ◽  
Nurul Azima Mazlan ◽  
Pei Teng Lum ◽  
Aina Akmal Mohd Noor ◽  
...  
Keyword(s):  
Zeta Potential ◽  
Mtt Assay ◽  
Cytotoxic Effect ◽  
Water Solubility ◽  
Biological Tissues ◽  
Assay Method ◽  
3T3 Cells ◽  
In Vivo Models ◽  
Dpph Method ◽  
Wide Range

Mangiferinis a xanthone glycoside, naturally isolated from Mangiferaindica. Mangiferinhas been reported for a wide range of pharmacological activities and its anticancer potential is very well known. However, the mangiferinanti-cancer potency isinadequate due to its poor water solubility. N,O-Carboxymethyl Chitosan (N,O-CMC) is a smart biopolymer, in which itsbiocompatible, biodegradable and non-toxic making itideal for abundant biological applications include the delivery of lipid soluble drugs. Also useful to improve and replace biological tissues and gene therapy. Hence, this study attempts to synthesize and characterize mangiferin-N,O-CMC nanoparticles and evaluate its antioxidant and cytotoxic properties. The mangiferin-N,O-CMC nanoparticles were prepared by loading mangiferin into N,O-CMC nanoparticles and characterized by FT-IR, DLS, SEM, Zeta potential and XRD measurements. In-vitro antioxidant was carried out by the DPPH method. The cytotoxic effect of mangiferin-N,O-CMC nanoparticles was carried out on Osteosarcoma MG-63 and 3T3 cells by using the MTT assay method. The synthesized mangiferin-N,O-CMC nanoparticles with particle size ranges from 200±10 nm. The charge of N,O-CMC nanoparticles were confirmed by Zeta potential and found to be −45.8 mV. In the DPPH method, mangiferin-N,O-CMC nanoparticles showed IC50 value between 8-16 µg/ml. In MTT assay, mangiferin-N,O-CMC nanoparticles exhibited a significant reduction in the growth of osteosarcoma MG-63 cells and there is no toxic effect against normal 3T3 cells. These findings designated that the synthesized mangiferin-N,O-CMC nanoparticles were very efficient nanocarrier in delivering the mangiferinto cancer cells. In the future, further studies with in-vivo models to be carried out on mangiferin-N,O-CMC nanoparticles to confirm its safety and effectiveness.

Cytotoxicity of Vincristine on the 5637 Cell Line Is Enhanced by Combination with Conferone

2009 ◽  
Vol 64 (5-6) ◽  
pp. 317-322 ◽  
Author(s):  
Vajiheh Neshatia ◽  
Maryam M. Matin ◽  
Mehrdad Iranshahi ◽  
Ahmad R. Bahrami ◽  
Javad Behravan ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cell Carcinoma ◽  
Mtt Assay ◽  
Cytotoxic Effect ◽  
Morphological Changes ◽  
Combined Treatment ◽  
Transitional Cell ◽  
Industrialized Countries ◽  
Histological Subtypes ◽  
Cytotoxicity Effects

Bladder cancer is one of the most common cancers worldwide, with the highest incidence in industrialized countries. There are three major histological subtypes of bladder cancer: transitional cell carcinoma (TCC) (>90%), squamous cell carcinoma (<10%) and adenocarcinoma (1 - 2%). The present study was carried out to assess the effects of conferone, a sesquiterpene coumarin isolated from Ferula badrakema, on a TCC subline, 5637 cells. In order to test the effects of conferone, 5637 cells were treated with different concentrations (16, 32, 64, 128 μg/ml) of conferone. The results indicated that conferone did not have any significant cytotoxic effect on these neoplastic cells. To determine the combining effects, the cells were cultured in the presence of different concentrations of conferone (16, 32, 64, 128 μg/ ml) and vincristine (30, 40, 50 μg/ml) in combination. The morphological changes were then observed and cytotoxicity effects were studied using the MTT assay 24, 48 and 72 h following drug administration. The cells were more rounded and granulated after treatments with both drugs in comparison to vincristine only. The results of the MTT assay confi rmed the morphological observations. After 48 h of combined treatment with 40 μg/ml vincristine and 16 μg/ml conferone, the cytotoxicity of vincristine was increased by 23.6%

scholarly journals ANTIPSORIASIS, ANTIOXIDANT, AND ANTIMICROBIAL ACTIVITIES OF AERIAL PARTS OF EUPHORBIA HIRTA

2018 ◽  
Vol 11 (9) ◽  
pp. 513
Author(s):  
Caroline Jeba R ◽  
Ilakiya A ◽  
Deepika R ◽  
Sujatha M ◽  
Sivaraji C
Keyword(s):  
Mtt Assay ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Assay Method ◽  
Coarse Powder ◽  
Euphorbia Hirta ◽  
Aerial Parts ◽  
Radical Scavenging Assay

Objectives: The aim of the study was curing antipsoriasis through Euphorbia hirta. The antipsoriasis activity was done by the 3-[4,5-dimethylthiazol- 2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay method.Methods: Aerial parts were shade, dry for 2 days, make into coarse powder and soaked in methanol for 72 h. The supernatant liquid was filtered by Whatman filter paper and condensed in a hot plate at 50°C. Dark gummy mass obtained. The study identified the antioxidant, antimicrobial, and antipsoriasis activities of methanol extract of aerial parts of E. hirta.Results: The IC50 value of methanol extract of aerial parts was found to be 72.20 μg/mL, 97.88 μg/mL, 55.88 μg/mL, and 36.31 μg/mL by 1,1-diphenyl- 2-picrylhydrazyl radical scavenging assay, superoxide radical scavenging assay, phosphomolybdenum reduction assay, and ferric (Fe3+) reducing power assay. The antipsoriasis activity was done by the MTT assay method. The maximum cell death was 88.37% observed at 0.781 μg/mL concentration and IC50 was 12.20 μg/mL concentrated.Conclusion: The results of the present investigation reveal the antipsoriasis activity of the extracts of E. hirta against bacteria and viruses, the psoriasis causing organisms. The methanol extract of E. hirta shows antioxidant, antimicrobial, and antipsoriasis activity and can be used to formulate a potential therapeutic agent for psoriasis.

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