Antimicrobial and Wound Healing Potential of Dietary Flavonoid Naringenin

2019 ◽  
Vol 9 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Ankita Tripathi ◽  
Himani Awasthi ◽  
Dan Bahadur Rokaya ◽  
Dipti Srivastava ◽  
Vivek Srivastava
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Tensile Strength ◽  
Agar Plate ◽  
Wound Contraction ◽  
Healing Index ◽  
Phenolic Constituents ◽  
Healing Property ◽  
Wound Healing Activity ◽  
Wound Healing Property

Background: Naringenin belongs to the class flavanones, and chemically it is known as 5,7-dihydroxy-2-(4-hydroxyphenyl) chroman-4-one. It is one of the dietary poly-phenolic constituents of the citrus fruits and vegetables. Naringenin possesses excellent antioxidant, antiinflammatory, and antimicrobial activities. These activities promote wound healing activity. Naringenin has been incorporated in the gel base to be applied on the wounded skin. The wound healing property of naringenin gel has not been studied extensively in the literature to date. </P><P> Objective: The present study aims to unveil the wound healing property of naringenin gel formulation on excision and incision model. </P><P> Materials and Methods: 0.5% and 1% naringenin was incorporated in a carbopol gel base formulation and evaluated for physicochemical parameters. Incision and excision wound models were used to study wound contraction, tensile strength, healing index, the period of epithelization and histopathological parameters. Antimicrobial activity was performed using agar plate method. Results: Naringenin (0.5% and 1%) treatment shows a significant increase in the percentage of wound contraction, good healing index and shorter epithelization period. Histopathology of naringenin gel treated wound tissue showed more fibroblasts, less macrophage infiltration, and wellformed collagen fibers as compared to control. In incision wound model, the animals were treated with 0.5% and 1% naringenin gel for nine days and showed a significant increase in the tensile strength. Naringenin exerts antimicrobial activity against fungus and E. coli. Conclusion: The present study explored potent wound healing activity of naringenin gels.

EVALUATION OF EXCISIONAL AND INCISIONAL WOUND HEALING ACTIVITY OF ELECTROHOMEOPATHIC DRUG (SPAGYRIC ESSENCE) GREEN ELECTRICITY IN RATS

2020 ◽  
pp. 72-75
Author(s):  
PRASANT KUMAR SABAT ◽  
SWETA PRIYADARSINI PRADHAN ◽  
RANDEEP PATRO
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Wound Contraction ◽  
Control Group ◽  
Albino Rats ◽  
Green Electricity ◽  
Wound Model ◽  
Wound Protection ◽  
Incision Wound ◽  
Wound Healing Activity

Objective: The current research is an attempt to evaluate the wound healing activity of Electrohomeopathic drug Green electricity (GE) in Wistar rats. No data have been reported till date on the wound healing effect of GE. Hence the present research focuses on the scientific investigation of the wound healing activity of GE in rats. Methods: Excision and incision wounds were inflicted upon albino rats. In the excision wound model, the wound contraction, Scar residue, area and time of complete epithelization were measured subsequently on day 0, 3,6,9,12,15, 18 and 21 after inflicting the wound. Wound contraction was calculated as a percentage change in the initial wound size. In the incision wound model, the tensile strength was measured on the 10th post wounding day. Results: It was noted that the effect produced by the GE Showed significant wound healing (p<0.01) in all wound models when compared to the control group. In the case of the excision model, GE statistically increases the wound contraction rate and in the case of the incision model GE showed high tensile strength as compared to the control group. In both cases the epithelization period was significantly decreased as compared to control. Almost 100% wound protection was achieved by GE in 21 d as compared to control. Conclusion: GE (ointment) showed significant wound healing activity (p<0.01) in both the excised wound model and incision wound model in rats.

scholarly journals IN VITRO WOUND HEALING AND ANTIMICROBIAL PROPERTY OF COTTON FABRICS COATED OPTIMIZED SILVER NANOPARTICLES SYNTHESIZED USING PELTOPHORUM PTEROCARPUM LEAF EXTRACTS

2019 ◽  
pp. 216-222
Author(s):  
ANNAMALAI P ◽  
BALASHANMUGAM P ◽  
KALAICHELVAN PT
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Incubation Temperature ◽  
Cotton Fabrics ◽  
Diffusion Method ◽  
Leaf Extracts ◽  
Potential Measurement ◽  
Healing Property ◽  
Wound Healing Activity ◽  
Wound Healing Property

Objective: The present study was aimed to study at investigating the antibacterial potential of silver nanoparticles (AgNPs) coated cotton fabrics against different pathogens and also for their wound healing property using fibroblasts cells. Materials and Methods: The leaf extracts of Peltophorum pterocarpum were used for the synthesis of AgNPs and were characterizing using ultraviolet-visible spectrophotometer, transmission electron microscopy, energy dispersive X-ray (EDX) spectroscopy, dynamic light scattering analysis, and zeta potential measurement. The AgNPs were coated on cotton fabrics and tested for their antibacterial efficacy using agar well diffusion method. The wound healing property of synthesized AgNPs was tested using fibroblast 3T3 cells. Results: The plant extracts of P. pterocarpum were utilized for AgNPs. The optimum condition for synthesizing AgNPs was found to be 1 mg/ml plant concentration, 7 pH, 1 mM silver nitrate concatenation, and incubation temperature of 37°C. The shape of synthesized AgNPs was found to be spherical with an average size between 20 and 50 nm, and elemental silver peaks were confirmed by EDX spectrum. The cotton fabrics coated with AgNPs show good zone of inhibition against all the tested pathogens and the treated fabrics were also characterized using scanning electron microscope which reveals the presence of AgNPs on the fabrics. The scratch assay reveals that the AgNPs have good wound healing activity when tested against fibroblast 3T3. Conclusion: The present results conclude that the synthesized AgNPs have good stability with potent antimicrobial activity when coated with cotton fabrics. The AgNPs also found to have good activity significant wound healing activity when tested using fibroblast cells.

scholarly journals Wound Healing Activity of Alcoholic Extract of Tamarix Aphylla L. on Animal Models

2019 ◽  
Vol 12 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Sajid Ali ◽  
Sarfaraz Alam ◽  
Sarfaraz Ahmad ◽  
Maksood Ali ◽  
Waquar Ahsan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Wound Healing ◽  
Tensile Strength ◽  
Ethanolic Extract ◽  
Activity Period ◽  
Tissue Homogenate ◽  
Alcoholic Extract ◽  
Standard Drug ◽  
Wound Healing Activity

To evaluate the wound healing activity of ethanolic extract of Tamarix aphylla L. on animal model. Wound creation like circular excision and linear incision method were considered for this study. The various parameters were studied like DNA estimation, total protein estimation, estimation of Hexosamine and Uronic acid, estimation of lipid peroxides and antioxidant activity, Tensile Strength of tissues from incision wounds, Antioxidant activity, Antimicrobial activity, Period of epithelialization and finally TNF-a concentration in the wound tissue homogenate were estimated. The treatment groups with the extract showed significant antimicrobial activity with compare to the standard drug. Significantly, 93. 86% increase in the collagen content and significant 52% up regulation in tensile strength was observed in the treated group. 40% reduction was observed in epithelialization period of the treated wounds. The results of the current study confirm that the ethanolic extract of T. aphylla has potent wound healing capacity.

scholarly journals Wound healing potential of Launaea pinnatiϔida Cass leaf juice in rats

2021 ◽  
Vol 12 (3) ◽  
pp. 2173-2177
Author(s):  
Kamala K. Chandak ◽  
Dipak D. Wasule
Keyword(s):  
Wound Healing ◽  
Dead Space ◽  
Skin Diseases ◽  
Breaking Strength ◽  
Scientific Work ◽  
Healing Property ◽  
Result Analysis ◽  
Wound Healing Activity ◽  
Leaf Juice ◽  
Wound Healing Property

Launaea pinnatifida is a procumbent herb native to the coastal area and found throughout India and many other countries. It is utilized as a dietary plant in the preparation of various dishes in the Maldives. Traditionally it is used for the treatment of skin injuries and is reported to contain flavonoids, steroids, glycosides, tannins, saponins etc. As no scientific work has been found to be published related to wound healing potential of the L. pinnatifida plant, the present study investigated the effects of dried leaf juice of L. pinnatifida on wound healing property in rats using various wound models. In result analysis, the wound contraction percentage was found to be increased and the scar area and epithelization time were found to be decreased when 3 % ointment L. pinnatifida leaf juice was applied topically. Similarly, with incision and dead space wound models, wound breaking strength and hydroxyproline levels were found to be increased. So, Launaea pinnatifida leaf juice showed significant wound healing activity in all wound models, which supports its claim for being used traditionally in skin diseases.

scholarly journals Phytochemical Screening and Wound Healing Activity of Different Leaf Extracts of Rhynchosia rothii in Rats

2021 ◽  
pp. 386-393
Author(s):  
Sharad D. Tayade ◽  
Narendra Silawat
Keyword(s):  
Wound Healing ◽  
Body Weight ◽  
Healing Process ◽  
Complete Healing ◽  
Wound Contraction ◽  
Wound Healing Process ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Healing Property ◽  
Wound Healing Activity

Aims: The aim of the present work to perform phytochemical screening and wound healing activity of different leaf extract of Rhynchosia rothii. Place and Duration of the Study: The present work has been carried out at Oriental University, Indore between the duration of November-2020 to January-2021. Methodology: Rhynchosia rothii dried leaf powder was subjected to continuous extraction with a soxhlet extractor using various organic solvents. Preliminary phytochemical screening with various qualitative chemical tests revealed the presence of Alkaloids, Tannins, Flavonoids, proteins, and mucilage present in Rhynchosia rothii leaf extracts. Wister rats of both sexes weighing 150-200 g were used for experimental purposes. The animals were kept in polypropylene cages at room temperature and with a light/dark cycle of 12:12 hours.  All the test extracts at a different dose level and standard were administered topically once daily from day 0 to the day of complete healing or the 21st postoperative day, whichever occurred earlier. Results: A significant increase in wound healing activity was observed in animals treated with alcoholic and aqueous extracts of Rhynchosia rothii leaves at a dose of 200 mg/kg body weight, compared to control treatments. Both methanolic and standard extracts of Rhynchosia rothii (200 mg/kg body weight) showed a dose-dependent increase in the rate of wound contraction. The methanolic extract of Rhynchosia rothii at a dose of 200 mg/kg body weight showed an increase in the percentage of wound contraction from 40.08% to 88.90% from day 3 to 12. The aqueous (standard) extract of Rhynchosia rothii at a dose of 200 mg/kg body weight showed an increase in the percentage of wound contraction from 46.02% to 100% from day 3 to 12. Conclusion: From the previous results it was concluded that the Wound represents a serious health problem, both in terms of morbidity and mortality. Wound healing is a critical response to tissue integrity. Therefore, the wound healing property of Rhynchosia rothii can be attributed to the phytoconstituents present in it, which may be due to its individual or additive effect that accelerates the wound healing process. Since Rhynchosia rothii is grown in abundance in India and rest of other part of world, it could be a fairly good and easily available therapeutic agent due to its naturally occurring tendency for healing wounds as a healer, as well as controlling abnormal healing.

scholarly journals Phytosomes of Naphthoquinone enriched Extract of Root bark of Onosma echioides exhibit wound healing activity in Rats

2021 ◽  
pp. 474-483
Author(s):  
JEEJA PANANCHERY ◽  
Chhaya Gadgoli
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Granulation Tissue ◽  
Petroleum Ether Extract ◽  
Treated Group ◽  
Wound Contraction ◽  
Root Bark ◽  
Wound Model ◽  
Wound Healing Effect ◽  
Wound Healing Activity

The present study is aimed at evaluation of phytosomal gel of the petroleum ether extract of root bark of Onosma echiodes for wound healing activity in rats. Extract of root bark of O. echioides was standardized by isolated naphthoquinone dimer using HPTLC. Phytosomes (equivalent to 2% w/w of naphthoquinones) of the standardized extract were prepared by thin film hydration technique. The wound healing efficacy of the formulation was evaluated in rats by inflicting excision and incision wounds followed by treatment of the wounds topically. The parameters evaluated for healing included determination of breaking strength and tensile strength of healed skin for incision model and percentage wound contraction, hydroxyproline content, granulation tissue free radicals and catalase in excision wound model. The formulation treated group showed a significant healing (p<0.005) of both the excision and incision wounds with respect to wound contraction and tensile strength respectively, as compared to vehicle treated group. The oxidative stress of the granulation tissue was also found to be reduced as indicated by reduced lipid peroxidation and increase in catalase activity. The phytosomal gel of O. echioides effectively exhibited wound healing effect.

WOUND HEALING ACTIVITY OF THE WHOLE PLANT OF MOMORDICA CHARANTIA LINN. IN RATS

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (11) ◽  
pp. 64-70
Author(s):  
M.P Singh ◽  
◽  
Y.S. Sarangdevot ◽  
S.S. Sisodia
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethanolic Extract ◽  
Momordica Charantia ◽  
Alcohol Extract ◽  
Whole Plant ◽  
Healing Property ◽  
Aqueous Ethanolic Extract ◽  
Wound Healing Activity

The aim of the present study was to explore the effect of wound healing activity of the whole plant of Momordica charantia Linn. in rats. The effect of aqueous ethanolic extract of Momordica charantia Linn. (Family; Cucurbetaceae) was evaluated on resutured incision, granuloma pouch and excision wound models in rats. The wound healing activity was measured by the rate of wound contraction, skin breaking strength, weight of the granulation tissue, and collagen content. The concentrated ethyl alcohol extract was subject to fractionation by using petroleum ether (40-60o V/V), solvent ether, ethyl acetate, butanol, butanone, ethanol and methanol in succession. All the fractions were screened for wound healing property. The results of pharmacological activity led to the conclusion that the petroleum ether fraction exhibited more significant wound healing promotion property followed by solvent ether and butanone, from all the parameters studied. Ethyl acetate, ethanol and methanol fractions were least effective.

Wound healing property of isolated compounds from Boesenbergia kingii rhizomes

2016 ◽  
Vol 184 ◽  
pp. 42-48 ◽  
Author(s):  
Teeratad Sudsai ◽  
Chatchai Wattanapiromsakul ◽  
Supinya Tewtrakul
Keyword(s):  
Wound Healing ◽  
Healing Property ◽  
Wound Healing Property
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