DYNAMICS OF RADIOGENIC CHANGES IN THE INTENSITY OF LIPID PEROXIDATION PROCESSES IN THE BLOOD OF ANIMALS UNDER DIFFERENT MODES OF IRRADIATION

2019 ◽  
pp. 100-104
Author(s):  
L. G. Petryna ◽  
M. I. Moiseienko
Keyword(s):  
Experimental Studies ◽  
Free Radical Oxidation ◽  
Radiation Dose Rate ◽  
Diene Conjugates ◽  
Radical Oxidation ◽  
Dose Rates ◽  
Wide Range ◽  
Male Wistar Rats ◽  
Peroxidation Processes ◽  
Relative Change

Experimental studies were performed on male Wistar rats. Single exposure of animals at doses of 1.0, 5.0, and 9.0 Gy was performed from a source with dose rates of 0.001, 0.01 0.1, and 1.0 Gy/min. The content of diene conjugates was determined at 0.5, 1, 2, 4, 6, 8, 10, 15, 20, and 30 days after exposure. It was found that the content of diene conjugates of blood varied wavy. The relative change in early products per 1 Gy irradiated at doses of 5.0 and 9.0 Gy was 1.5-2.0 and 4-5 times lower, respectively, than in animals irradiated at dose 1.0 Gy. After 4, 6, 8, 10 days, a clear dependence of the indicator on the magnitude of the radiation dose rate was not observed. The dynamics of accumulation of early LPO R products in the blood of rats, irradiated over a wide range of doses and capacities, indicates the nature of the development or inhibition of free radical oxidation processes.

DYNAMICS OF RADIOGENIC CHANGES IN THE INTENSITY OF LIPID PEROXIDATION PROCESSES IN THE BLOOD OF ANIMALS UNDER DIFFERENT MODES OF IRRADIATION

2019 ◽  
pp. 100-104
Author(s):  
L. G. Petryna ◽  
M. I. Moiseienko
Keyword(s):  
Experimental Studies ◽  
Free Radical Oxidation ◽  
Radiation Dose Rate ◽  
Diene Conjugates ◽  
Radical Oxidation ◽  
Dose Rates ◽  
Wide Range ◽  
Male Wistar Rats ◽  
Peroxidation Processes ◽  
Relative Change

Experimental studies were performed on male Wistar rats. Single exposure of animals at doses of 1.0, 5.0, and 9.0 Gy was performed from a source with dose rates of 0.001, 0.01 0.1, and 1.0 Gy/min. The content of diene conjugates was determined at 0.5, 1, 2, 4, 6, 8, 10, 15, 20, and 30 days after exposure. It was found that the content of diene conjugates of blood varied wavy. The relative change in early products per 1 Gy irradiated at doses of 5.0 and 9.0 Gy was 1.5-2.0 and 4-5 times lower, respectively, than in animals irradiated at dose 1.0 Gy. After 4, 6, 8, 10 days, a clear dependence of the indicator on the magnitude of the radiation dose rate was not observed. The dynamics of accumulation of early LPO R products in the blood of rats, irradiated over a wide range of doses and capacities, indicates the nature of the development or inhibition of free radical oxidation processes.

scholarly journals Peculiarities of lipid peroxidation processes in the body of cats with urinary tract diseases and their correction with commercial feeds

2021 ◽  
pp. 37-40
Author(s):  
Olga Andreevna Vorontsova ◽  
Nikolay Alexandrovich Pudovkin ◽  
Vladimir Vasilievich Salautin ◽  
Sergey Dmitrievich Klyukin
Keyword(s):  
Urinary Tract ◽  
Blood Serum ◽  
Catalase Activity ◽  
Free Radical Oxidation ◽  
The Body ◽  
Diene Conjugates ◽  
Radical Oxidation ◽  
Mda Content ◽  
Peroxidation Processes ◽  
Commercial Feeds

The article presents the results of studies on the effect of commercial food on the processes of free radical oxidation of lipids in the body of cats with urinary tract pathology. It has been found that the concentration of diene conjugates in the blood serum of sick animals increases significantly by 15.1 - 36.7% relative to healthy ones. The MDA content in the blood serum of healthy animals was 6.05 ± 0.28 nmol / g. In animals with urolithiasis, this indicator increased by about 2 times. When feeding with commercial food and homemade food, LPO processes normalize, which is most pronounced on the 21st day. The average activity of catalase in the blood serum of sick animals was increased from 30.6% to 40.2% relative to healthy ones (15.15 ± 0.61 ?mol / ml). After feeding with therapeutic and prophylactic fodders, by the 7th day, the catalase activity did not significantly decrease compared to the initial level, but remained higher than in control animals by 19.6% - 37.1%. On the 14th day, the catalase activity did not change significantly compared with the 7th day and was higher than the control values by 21.5% - 27.5%.

scholarly journals Chemiluminescence Studies of Lipoperoxidation Processes in Arterial Hypertension and Stress

2019 ◽  
Vol 4 (1) ◽  
pp. 133-137
Author(s):  
Anastasiya S. Brichagina ◽  
Mariya I. Dolgikh ◽  
Larisa R. Kolesnikova ◽  
Larisa V. Natyaganova
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
The State ◽  
Male Rats ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Biochemical Methods ◽  
Peroxidation Processes

Various pathologies, in particular hypertension, accompany violation of the processes of free radical oxidation. The state of the system lipid peroxidation – antioxidant protection can be assessed only by a complex of biochemical methods. The chemiluminescent method is used to obtain a faster result. The method has a number of advantages: measurement of chemiluminescence parameters is carried out in natural conditions and does not require sample preparation, is highly selective, it can be used to identify the presence of free radical pathology, analysis of the intensity of lipid peroxidation processes, the effectiveness of treatment with antioxidant medicament. The aim of the research was to study the lipid peroxidation under hypertension and stress by chemiluminescence. The experiment was conducted on male rats of the ISIAH line. The experiment consisted of 2 stages: stage of alarm and stage of chronic stress. We found that, depending on the type of stress, the state of the system lipid peroxidation-antioxidant protection differed. The role of stress in violation of the processes of lipoperoxidation in hypertension was established. The efficacy of the chemiluminescence method as an alternative to a complex of biochemical methods is shown. Express assessment of pro- and antioxidant status is possible for patients with hypertension.

scholarly journals Effects of His-Phe-Arg-Trp-Pro-Gly-Pro peptide on free-radical oxidation processes in conditions of chronic restraint stress

2021 ◽  
Author(s):  
AO Vorvul ◽  
II Bobyntsev ◽  
OA Medvedeva ◽  
IuE Azarova ◽  
AE Belykh ◽  
...  
Keyword(s):  
Free Radical ◽  
Restraint Stress ◽  
Free Radical Oxidation ◽  
Regulatory Peptides ◽  
Thiobarbituric Acid ◽  
Synthetic Analog ◽  
Chronic Restraint Stress ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Wide Range

Studying the effects of regulatory peptides on the stress-induced shifts in the bodily processes is of great fundamental and applied significance. Currently, a wide range of peptide neurotropic drugs, affecting the stress response development, are used in medicine, and new promising molecules are being studied. The study was aimed to assess the effects of the adrenocorticotropic hormone (ACTH) synthetic analog, ACTH(6-9)-Pro-Gly-Pro, administered at a dose of 5, 50 and 500 μg/kg, on the free-radical oxidation processes in Wistar rats, subjected to chronic restraint stress (CRS) during two weeks. Serum levels of 8-oxo-2'-deoxyguanosine (8-OHdG) and superoxide dismutase 3 (SOD3) were assessed by enzyme immunoassay, and the levels of thiobarbituric acid reactive substances (TBARS) were assessed by fluorimetric method. CRS lead to the significant increase in the 8-OHdG levels by 18.4% (p = 0.01) and the decrease in the SOD3 levels by 14.3% (p = 0.01), however, it had no effect on the levels of TBARS. ACTH(6-9)-Pro-Gly-Pro, administered at a dose of 5 and 50 μg/kg, significantly decreased the levels of 8-OHdG by 19.8% (p = 0.03) and 30% (p = 0.001), respectively. Thus, it was found that CRS resulted in oxidative stress in animals. ACTH(6-9)-Pro-Gly-Pro administration at a dose of 5 and 50 μg/kg inhibits the stress-induced free-radical oxidation processes.

scholarly journals Correction of antioxidant protection of cows organism in theperiod of laboratory drugs with content of non-participants of metals

2018 ◽  
Vol 20 (83) ◽  
pp. 204-207
Author(s):  
I.B. Kobilyukh
Keyword(s):  
Antioxidant System ◽  
Free Radical Oxidation ◽  
The Body ◽  
Control Group ◽  
Antioxidant Protection ◽  
Dry Period ◽  
Diene Conjugates ◽  
Radical Oxidation ◽  
Black And White ◽  
White Breed

The enhancement of free radical oxidation, characteristic of inflammatory processes, is accompanied by a violation of the properties of biological membranes, the functioning of cells and damage to surrounding tissues. The purpose and task of the research was to make correction of antioxidant protection of the body of cows during the period of dry preparations containing nanoparticles of metals. To activate the antioxidant system of the body of cows during the dry period, use the vitamin complex «Nanovit». The experiment was conducted on cows of Ukrainian dairy black-and-white breed in Agroprodservice-Invest LLP of the Kozivsky District of the Ternopil Oblast. The experiment was conducted before 35–25 days to the calves birth where the experimental and control groups of cows were formed (n = 10). Cows of the experimental group for 35–25 days to the cattle, intramuscularly injected 5.0 ml vitamin complex «Nanovit» (composition: vitamins A, D, E and nanoparticles Cu, Zn, Mn, Co). Producer of «NVP» LLC «Ecological Capital». Cows of the control group for 35–25 days to the catheter, intramuscularly administered 15.0 ml of the drug Prodevit-tetra (1 ml of the preparation contains vitamin A – 50000 IU, vitamin D – 25000 IU, vitamin E – 20 mg, vitamin F – 6 mg). Manufacturer of Product Ltd. The study found that vitamins A, D, E, and nanoparticles Cu, Zn, Mn, Co, which are part of the drug «Nanovit», activate the antioxidant system of the body of cows during the dry period. So after the application of the drug «Nanovit» in the body of cows there was a decrease of 58.5% (Р ≤ 0.01) of diene conjugates and 95.0% (Р ≤ 0.001) of TBK-active products against the background of an increase of 20.0% (Р ≤ 0.01) activity of catalase. After the application of the drug «Nanovit», the service period of the period from cows decreased by 54 days, and the index of insemination decreased by 0.2 units against the cows fed «Prodevit-tetra», indicating a positive effect of the drug containing the nanoparticles of metals Cu, Zn, Mn, Co and vitamins A, D, E during the post-period period in cows.

scholarly journals Influence of the preparation “Bendamin” on the indicators of antioxidant protection of rat myocardium in experimental modeling of heart failure

2019 ◽  
Vol 21 (95) ◽  
pp. 98-101
Author(s):  
I. S. Varkholiak ◽  
B. V. Gutyj
Keyword(s):  
Intraperitoneal Administration ◽  
Clinical Signs ◽  
Free Radical Oxidation ◽  
Feed Additives ◽  
Control Group ◽  
Standard Diet ◽  
Antioxidant Protection ◽  
Diene Conjugates ◽  
Radical Oxidation ◽  
Experimental Group

The aim of this study was to investigate the effect of Bendamin on the antioxidant status of rats in experimental doxorubicin-induced cardiomyopathy. The studies were performed on white sexually mature young male of Vistar line rats weighing 180–200 g, which were kept on a standard diet of the Institute Vivarium of the State Research Institute of Veterinary Drugs and Feed Additives. Animals were divided into 3 groups of 6 animals in each: control group – intact animals; experimental group R1, in which animals were simulated with doxorubicin-induced cardiomyopathy by intraperitoneal administration of doxorubicin at a dose of 2.5 mg/kg 3 times a week for two weeks; experimental group R2, in which animals were injected with the drug “Bendamin” at a dose of 20 mg/kg after injection of doxorubicin. The data obtained indicate that the introduction into the experimental animals of the experimental group of doxorubicin is accompanied by intensification in the myocardium of the processes of free radical oxidation. The increase in diene conjugates was found to be 32.7% and TBK-active products increased by 37.6% compared to the control group of rats. Doxorubicin was also found to cause inhibition of the glutathione system of the animal's antioxidant protection after administration to rats. After administration of the drug “Bendamin”, rats of the second experimental group were suppressed with lipid peroxidation under the conditions of doxorubicin intoxication. In the rats of the second experimental group, the level of intermediates and end products is likely to decrease, so in the rat myocardial homogenate the level of diene conjugates decreased by 16.8% and the level of TBA-active products by 20.8% compared to the first experimental group. who had clinical signs of cardiomyopathy caused by doxorubicin administration. The use of the drug “Bendamin” in rats of the second experimental group contributed to the enhancement of the enzymatic and non-enzymatic element of the antioxidant system, protecting the structural and functional integrity of cell bioembranes. The results of the studies enrich the pharmacological characteristics of the drug “Bendamin”, indicate its sufficiently clear protective effect on the myocardium in experimental doxorubicin cardiomyopathy and is a convincing proof of the feasibility of the drug in veterinary practice.

scholarly journals Metformin reduces urate nephropathy in experimental nephrolithiasis

2017 ◽  
Vol 8 (4) ◽  
pp. 644-648
Author(s):  
V. Y. Perfil’ev ◽  
Y. F. Zverev ◽  
D. Y. Perfil’eva ◽  
I. V. Lysenko ◽  
A. G. Miroshnichenko
Keyword(s):  
Metabolic Syndrome ◽  
Classical Model ◽  
Free Radical Oxidation ◽  
Developed Countries ◽  
Ammonium Transport ◽  
Urine Ph ◽  
Lactate Dehydrogenase Activity ◽  
Radical Oxidation ◽  
Urate Nephropathy ◽  
Male Wistar Rats

The objective of the article is to evaluate the effectiveness of metformin in the prevention and treatment of experimental urate nephropathy. About 33% of the populations of developed countries suffers from metabolic syndrome. The relationship between metabolic syndrome, especially insulin resistance, with gout and urate nephrolithiasis, is now proven. The search for a common pathophysiological link in the development of these conditions allows us to identify insulin-dependent excessive urinary acidification due to impaired education and renal ammonium transport. We suggested the use of drugs that can increase the sensitivity of tissues to insulin, and induce a decrease in the manifestations of urate nephropathy. The study was performed on 30 male Wistar rats weighing 250–300 g. For the induction of urate nephropathy in rats, we used the classical model of inhibition of uricase by oxononium acid. Metformin was administered at a dose of 150 mg/kg in the treatment and prophylactic regimens. It was found that the use of metformin both in prophylactic and therapeutic regimen leads to a reliable decrease the level of uric acid in blood plasma and urine in rats with experimental urate nephrolithiasis. We found that the preventive use of metformin brought significant relief after experimental urate nephropathy, as evidenced by a shift in urine pH to the alkaline side, a decreased lactate dehydrogenase activity in urine, as well as a decrease in the processes of free radical oxidation in the blood and in the kidneys of the animals. 

scholarly journals Critical conditions as a logical and appropriate chain of iron methabolism disorders (the summary of experimental studies)

2013 ◽  
Vol 59 (6) ◽  
pp. 700-709 ◽  
Author(s):  
Y.P. Orlov ◽  
V.N. Lukach ◽  
V.T. Dolgih ◽  
E.L. Soboleva ◽  
A.V. Ivanov ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Rheological Properties ◽  
Experimental Studies ◽  
Free Radical Oxidation ◽  
Critical Conditions ◽  
Total Antioxidant Activity ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Total Antioxidant

It is revealed that in all models of critical conditions will activate the free-radical oxidation, decreasing the total antioxidant activity, the concentration of transferrin decreases in the serum of the blood, increase of the concentration of ferritin, the rheological properties of the blood are violated and the signs of endothelial dysfunction are identified. Pre-entered deferoxamine in the dose of 80 mg/kg reduced the intensity of free-radical oxidation processes, restoring the antioxidant potential, concentration of the transferrin, and a lower level of ferritin, contributed to the normalization of blood rheological properties and a reduction of the extent endothelium destruction as a result of the reduction Fe2+ concentration in blood serum.

scholarly journals Changes of redox reactions in the lung tissue under burn injury and combined application of lyophilized xenographt substrate and exogenous surfactant preparation

2015 ◽  
Vol 17 (3) ◽  
Author(s):  
Z. M. Nebesna ◽  
К. S. Volkov ◽  
N. Ye. Lisnychuk ◽  
І. Ya. Demkiv
Keyword(s):  
Redox Reactions ◽  
Burn Injury ◽  
Free Radical Oxidation ◽  
The Body ◽  
Exogenous Surfactant ◽  
Radical Oxidation ◽  
Experimental Animals ◽  
Combined Application ◽  
Surfactant Preparation ◽  
Peroxidation Processes

<p>Oxidative stress in the body of the experimental animals after burn injury arises and develops on the background<br />of activation of free radical oxidation and decrease of antioxidant activity and increase of endotoxemia. The combined<br />use of surfactant preparation and lyophilized xenographt substrate after skin burns lead to a significant decrease of<br />endotoxemia, inhibition of lipid peroxidation processes and restoring functional activity of antioxidant protection.</p>

scholarly journals Effect of Leu-Ile-Lys tripeptide on the activity of free radical oxidation and expression of сyclooxygenase-1 and -2 in gastric mucosa in experimental indomethacin-induced ulcer in rats

2019 ◽  
Vol 100 (3) ◽  
pp. 439-444
Author(s):  
S E Lorents ◽  
E N Mazko ◽  
O G Makarova ◽  
A Yu Zharikov ◽  
Yu V Korenovskiy ◽  
...  
Keyword(s):  
Free Radical ◽  
Gastric Mucosa ◽  
Free Radical Oxidation ◽  
Control Group ◽  
Intragastric Administration ◽  
Radical Oxidation ◽  
Cyclooxygenase 1 ◽  
Total Antioxidant ◽  
Male Wistar Rats

Aim. To determine the effect of Leu-Ile-Lys tripeptide on indicators of oxidative stress and expression of cyclooxygenase-1 and -2 in the gastric mucosa on the background of experimental indomethacin-induced ulcer. Methods. Experiments were performed on 35 male Wistar rats with a body mass of 200-250 g aged 2-3 months that were divided into 3 groups: the control group (intact rats, 8 animals), control group (simulation of indomethacin-induced gastropathy, 12 animals) experimental group (simulation of indomethacin-induced gastropathy + administration of Leu-Ile-Lys tripeptide, 15 animals). The tripeptide Leu-Ile-Lys obtained by chemical synthesis (sample purity at least 98%) was administered intragastrically daily for 7 days before the simulation of indomethacin-induced damage of the gastric mucosa in a dose of 11.5 mg/kg. The model of indomethacin-induced damage to the rodent gastric mucosa was reproduced by a single intragastric administration of indomethacin in a dose of 60 mg/kg in 1 ml of saline. In the stomach homogenate the activity of free radical oxidation was determined by conventional methods. For quantitative determination of cyclooxygenase-1, -2 in gastric homogenate the method of enzyme immunoassay was used. The concentration was determined spectrometrically by the color intensity of the samples. Results. With a prophylactic administration of tripeptide Leu-Ile-Lys, concentration of tiobarbiturate-reactive products was normalized, total antioxidant activity increased and the activity of antioxidant enzymes decreased compared to the control group. The concentration of cyclooxygenase-2 in gastric homogenate from the experimental animals was 2.3 times lower than that of the control rats. Conclusion. Use of Leu-Ile-Lys tripeptide in experimental indomethacin-induced gastric ulcer showed a significant decrease of oxidative damage and inflammation in the gastric mucosa.

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