THE EFFECT OF OINTMENTS WITH ANTIOXIDANT ACTIVITY ON THE MORPHOLOGICAL STRUCTURES OF SKIN OF GUINEA PIGS EXPOSED TO LOCAL ULTRAVIOLET IRRADIATION

2019 ◽  
pp. 64-72
Author(s):  
T. V. Zvyagintseva ◽  
S. I. Myronchenko ◽  
N. I. Kytsyuk ◽  
O. V. Naumova
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Ultraviolet Irradiation ◽  
Guinea Pigs ◽  
Main Group ◽  
The State ◽  
Elastic Fibers ◽  
Control Group ◽  
General Tendency ◽  
Skin Reactions

Considering the particular danger of remote skin reactions to ultraviolet irradiation (UVI), it is advisable to use ointments with antioxidant activity to reduce its negative effect on the skin. The rationale for the choice of ointments with antioxidant activity was the fact that they reduce the damaging effect of ultraviolet radiation in the erythemal and early post-erythemal period. The presence of a regular connection between the development of the early and late periods has given reason to assume the protective effect of ointments on the remote skin reactions. Objective: to study the effect of thiotriazoline ointment and thiotriazoline ointment with silver nanoparticles on the state of the morphological structures of the skin of guinea pigs after local UVI. Material and methods of research. The study involved 132 albino guinea pigs weighing 400-500 g, divided into 4 groups: 1 - intact, 2 - control (guinea pigs subjected to local UVI), 3 and 4 main ones. The third main group included guinea pigs that after UVI were administered thiotriazoline ointment in the treatment and prophylactic regime, the fourth main group included guinea pigs that after UVI were administered thiotriazoline ointment with silver nanoparticles in the same mode as Group 3. Ointments were applied 1 hour before irradiation and daily until erythema disappeared. Ultraviolet erythema was caused by irradiation in 1 minimum erythemal dose. After 2, 4 hours, on the 3rd, 8th, 15th, 21st, 28th day, the fragments of irradiated skin were investigated using histochemical and morphometric methods (fibroblast density and epidermis thickness). Results. Morphological changes in the skin after applying ointments with antioxidant activity were unidirectional. It was revealed that in the early periods after irradiation, thiotrazoline ointment and thiotrazoline ointment with silver nanoparticles do not affect changes in the thickness of the epidermis, but statistically significantly reduce the density of fibroblasts in the dermis on the 3rd day of the experiment compared to the control group. In the later periods, under the influence of thiotriazoline ointment, a gradual decrease in the thickness of the epidermis, which reached the norm by the end of the experiment, was observed. On the 8th day, the maximum density of fibroblasts was recorded, in the subsequent periods of the experiment, the index gradually decreased, which was accompanied by collagenization of the papillary layer in the loci of damage to collagen and elastic fibers detected in 50% of cases. In later times, under the influence of thiotriazoline ointment with silver nanoparticles, the processes of restoring the morphological structures of the skin occurred faster. In parallel with the decrease in the density of fibroblasts in the loci of the previous damage to the collagen and elastic fibers of the papillary layer, thickening of collagen fibers was observed, replacing them with segments of destruction of elastic fibers. In this group, at the end of the experiment, the collagenization locus was small, single, occurring in 16.7% of cases. Conclusions Ointments with antioxidant activity exert a positive effect on the state of morphological structures of the skin, damaged as a result of local UVI, in erythemal and post-erythemic periods. In the early periods after the local UVI, there was a general tendency for the effect of both ointments, as they reduced the density of fibroblasts on the 3rd day, but did not result in complete normalization. In the late period after local UVI , under the influence of thiotriazoline ointment and thiotriazoline ointment with silver nanoparticles, thickness of the epidermis (by 21st and 15th day, respectively) and density of fibroblasts (by the 28th day) decreased to normal while without treatment both indicators exceeded the norm by several times for 28 days of the experiment.

THE EFFECT OF OINTMENTS WITH ANTIOXIDANT ACTIVITY ON THE MORPHOLOGICAL STRUCTURES OF SKIN OF GUINEA PIGS EXPOSED TO LOCAL ULTRAVIOLET IRRADIATION

2019 ◽  
pp. 64-72
Author(s):  
T. V. Zvyagintseva ◽  
S. I. Myronchenko ◽  
N. I. Kytsyuk ◽  
O. V. Naumova
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Ultraviolet Irradiation ◽  
Guinea Pigs ◽  
Main Group ◽  
The State ◽  
Elastic Fibers ◽  
Control Group ◽  
General Tendency ◽  
Skin Reactions

Considering the particular danger of remote skin reactions to ultraviolet irradiation (UVI), it is advisable to use ointments with antioxidant activity to reduce its negative effect on the skin. The rationale for the choice of ointments with antioxidant activity was the fact that they reduce the damaging effect of ultraviolet radiation in the erythemal and early post-erythemal period. The presence of a regular connection between the development of the early and late periods has given reason to assume the protective effect of ointments on the remote skin reactions. Objective: to study the effect of thiotriazoline ointment and thiotriazoline ointment with silver nanoparticles on the state of the morphological structures of the skin of guinea pigs after local UVI. Material and methods of research. The study involved 132 albino guinea pigs weighing 400-500 g, divided into 4 groups: 1 - intact, 2 - control (guinea pigs subjected to local UVI), 3 and 4 main ones. The third main group included guinea pigs that after UVI were administered thiotriazoline ointment in the treatment and prophylactic regime, the fourth main group included guinea pigs that after UVI were administered thiotriazoline ointment with silver nanoparticles in the same mode as Group 3. Ointments were applied 1 hour before irradiation and daily until erythema disappeared. Ultraviolet erythema was caused by irradiation in 1 minimum erythemal dose. After 2, 4 hours, on the 3rd, 8th, 15th, 21st, 28th day, the fragments of irradiated skin were investigated using histochemical and morphometric methods (fibroblast density and epidermis thickness). Results. Morphological changes in the skin after applying ointments with antioxidant activity were unidirectional. It was revealed that in the early periods after irradiation, thiotrazoline ointment and thiotrazoline ointment with silver nanoparticles do not affect changes in the thickness of the epidermis, but statistically significantly reduce the density of fibroblasts in the dermis on the 3rd day of the experiment compared to the control group. In the later periods, under the influence of thiotriazoline ointment, a gradual decrease in the thickness of the epidermis, which reached the norm by the end of the experiment, was observed. On the 8th day, the maximum density of fibroblasts was recorded, in the subsequent periods of the experiment, the index gradually decreased, which was accompanied by collagenization of the papillary layer in the loci of damage to collagen and elastic fibers detected in 50% of cases. In later times, under the influence of thiotriazoline ointment with silver nanoparticles, the processes of restoring the morphological structures of the skin occurred faster. In parallel with the decrease in the density of fibroblasts in the loci of the previous damage to the collagen and elastic fibers of the papillary layer, thickening of collagen fibers was observed, replacing them with segments of destruction of elastic fibers. In this group, at the end of the experiment, the collagenization locus was small, single, occurring in 16.7% of cases. Conclusions Ointments with antioxidant activity exert a positive effect on the state of morphological structures of the skin, damaged as a result of local UVI, in erythemal and post-erythemic periods. In the early periods after the local UVI, there was a general tendency for the effect of both ointments, as they reduced the density of fibroblasts on the 3rd day, but did not result in complete normalization. In the late period after local UVI , under the influence of thiotriazoline ointment and thiotriazoline ointment with silver nanoparticles, thickness of the epidermis (by 21st and 15th day, respectively) and density of fibroblasts (by the 28th day) decreased to normal while without treatment both indicators exceeded the norm by several times for 28 days of the experiment.

scholarly journals EFFECT OF LOCAL UV RADIATION ON THE MORPHOFUNCTIONAL STATE OF THE SKIN OF GUINEA PIGS IN EARLY AND REMOTE PERIODS AFTER IRRADIATION

2020 ◽  
Vol 73 (4) ◽  
pp. 705-707
Author(s):  
Tetyana V. Zvyagintseva ◽  
Svitlana I. Myronchenko ◽  
Nataliia I. Kytsiuk ◽  
Olga V. Naumova
Keyword(s):  
Ultraviolet Irradiation ◽  
Guinea Pigs ◽  
Acute Inflammation ◽  
Elastic Fibers ◽  
Control Group ◽  
Entire Observation Period ◽  
Morphological Picture ◽  
Morphometric Methods ◽  
Observation Period

The aim is to establish the features of morphological and morphometric changes in the skin of guinea pigs in erythemal, early post-erythemal and late post-erythemic periods after local ultraviolet irradiation. Materials and methods: Studies were conducted on 54 albino guinea pigs weighing 400-500 g. Ultraviolet erythema was caused by irradiation in 1 minimum erythemal dose. The control group included intact guinea pigs. After 2, 4 hours, on the 3rd, 8th, 15th, 21st, 28th day, the fragments of the irradiated skin were investigated using histochemical and morphometric methods. Results: After 2, 4 hours after irradiation, dyscirculatory changes in the skin develop. By the 3rd day of the experiment a morphological picture of acute inflammation in the epidermis and dermis develops, apoptotic keratinocytes appear (sunburn cells), which is accompanied by thickening of the epidermis and an increase in the density of fibroblasts. By the 8th day proliferative-hyperplastic and degenerative changes begin to prevail, including dystrophic nature, the thickness of the epidermis and the density of fibroblasts reach a maximum. In the long term, on the 15-28th day, dystrophic changes of the epidermis, dyskeratosis, changes in the number and structure of elastic fibers with an increase in uneven fibrosis, collagenization processes and the development of sclerotic changes, as well as a significant thickening of the epidermis, an increase in the density of fibroblasts are observed. Conclusions: The data obtained indicate pronounced morphofunctional changes in the skin in the zone of local ultraviolet irradiation observed throughout the entire observation period.

Antioxidant status of blood in patients with vibration disease

2019 ◽  
pp. 978-982 ◽  
Author(s):  
N. N. Malyutina ◽  
A. F. Bolotova ◽  
R. B. Eremeev ◽  
A. Zh. Gilmanov ◽  
D. Yu. Sosnin
Keyword(s):  
Antioxidant Activity ◽  
Blood Serum ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Main Group ◽  
Biologically Active ◽  
Antioxidant Systems ◽  
Control Group ◽  
Vibration Disease ◽  
Total Antioxidant

Introduction. The overwhelming number of publications contains only data on the content of individual antioxidants, but not on the overall antioxidant activity of the blood in patients with vibration disease.The aim of the study was to determine the total antioxidant activity of blood serum in patients with vibration disease.Materials and methods. Th e main group consisted of 30 people diagnosed with “Vibration disease” of 1 degree (n=21) and 2 degrees (n=9). Th e control group consisted of 30 clinically healthy men, comparable in age with the main group (p=0.66). Th e total activity of antioxidant systems of blood plasma was evaluated photometrically using the test system “Total antioxidant status-Novo” (“Vector-best”, Russia).Results. The indicator of the total antioxidant status (TAS) was 1,038±0.232 mmol/l in the examined main group, against 1,456±0.225 mmol/l in the examined control group (p<0.000001). Th e coefficient of variation (CV) in patients with vibration disease was 22.35%, 1.45 times higher than in the control group (15.45%). In the main group there was a positive correlation between age and TAS (R=0.525), in the control group there was no such relationship (R=0.095). Th e degree of decrease depended on the severity of vibration disease.Conclusions. 1. The development of vibration disease is accompanied by a decrease in the antioxidant status of blood serum. 2. Th e degree of decrease in the antioxidant status of blood serum correlates with the severity of vibration disease. 3. Reduction of TAS can serve as a pathogenetic justification of the need to include drugs and/or biologically active additives with antioxidant activity in therapy

scholarly journals ASSESSMENT OF THE NATURE OF THE MICROCIRCULATORY RESPONSE TO EXPERIMENTAL THERMAL INJURY

2020 ◽  
pp. 123-129
Author(s):  
А.К. Martusevich ◽  
◽  
А.А. Yepishkina ◽  
L.R. Dilenyan ◽  
◽  
...  
Keyword(s):  
Blood Flow ◽  
Thermal Injury ◽  
Negative Impact ◽  
Small Diameter ◽  
Main Group ◽  
The State ◽  
Border Zone ◽  
Control Group ◽  
Thermal Burn ◽  
Doppler Flowmetry

Burn disease affects the functioning of almost all functional systems, having a negative effect on them due to the development of endotoxicosis and vascular disorders. At the same time, the greatest attention is paid to the shifts that form in the systemic hemodynamics, primarily in the coronary and pulmonary blood flow, which can cause the development and progression of life-threatening conditions. At the same time, the microcirculatory system is also undergoing changes, but these changes have been studied in much less detail. The aim of this research was to study the state of skin microcirculation in healthy and burned rats. The experiment was carried out on 20 adult male Wistar rats divided into two equal groups. The first group of animals (n=10) was a control group (intact), with its representatives there were not any manipulations, except for a single study of the state of microcirculation. Rats of the second (main) group (n=10) were simulated contact thermal burn using their own patented method. The assessment of microcirculation parameters in the main group of animals was carried out by laser Doppler flowmetry on the device "LAKK-M" on the 1st day (2-3 hours after the injury), on the third and tenth days after the burn period. It was found that thermal injury has a negative impact on the microcirculation system, which is seen in a decrease in the intensity of blood flow through small-diameter vessels. This is achieved by reflex vasospasm, reducing the release of nitrogen oxide by endothelial cells and activating precapillary sphincters, and simultaneously activating the shunting ways of cardiac shunt from the arterial directly to the venous channel, bypassing the capillaries. Thus, in the post-thermal period, a compensatory "robbing phenomenon" is realized in the border zone of the burn, which requires pathogenetic correction.

Change of skin electrical activity during osteopathic correction with the use of biodynamical approach

2019 ◽  
pp. 85-92
Author(s):  
Yu. O. Novikov ◽  
A. R. Shaiakhmetov ◽  
G. M. Musina
Keyword(s):  
Nervous System ◽  
Autonomic Nervous System ◽  
Electrical Activity ◽  
Main Group ◽  
The State ◽  
The Body ◽  
Control Group ◽  
Autonomic Nervous ◽  
Contact Exposure ◽  
Correlation Coeffi

Introduction. In connection with the widespread introduction of osteopathy into practical medicine, new facts confi rming its effectiveness are appearing. These facts need theoretical justifi cation. The biodynamic approach is the least studied and scientifi cally proven approach in osteopathy. There are only a few works describing the benefi cial effects of this type of treatment on the autonomic nervous system based on an assessment of heart rate variability. One of the most sensitive indicators of changes in the state of the body is the electrical activity of the skin (EAS), which is widely used in modern polygraphic devices.Goal of research — to investigate the EAS in the physician and in the patient during the session of craniosacral biodynamics (CSB).Materials and methods. The study involved 32 patients who were randomly divided into 2 groups — control group (10 patients) and main group (22 patients). In the control group there were 8 women and 2 men aged from 20 to 65 years (mean age 42±20 years). In the main group, 16 women and 6 men aged from 18 to 68 years (mean age 40±16 years) were examined. Two professional certifi ed computerized polygraphs «Chris» were used, simultaneously recording the EAS values of the doctor and the patient. In the main group, the background indicators were recorded at the fi rst stage (10 minutes), then the procedure of the CSB, which lasted 20 minutes, was recordered. The control group was used only for registration of EAS. At the same time, patients were in the same conditions as in the main group.Results. During the CSB, the state of both the doctor (p<0,001) and the patient (p<0,001) has changed signifi cantly. At the same time, the tendency of changes in individual indicators of EAS in patients during treatment was logical. The altered state of the physician practically did not depend on its original level (p>0,05). However, there was a high relationship between the states of the doctor during contact and non-contact treatment (correlation coeffi cient 0,80, p<0,001). The patient′s initial condition had a moderate, statistically signifi cant relationship with his condition during the treatment (p<0,001). Since the beginning of the craniosacral biodynamics, the patient′s condition has hardly changed. At the same time, the contactless impact on the patient′s body was comparable with the contact effect (correlation coeffi cient 0,97, p<0,001). There was no signifi cant relationship between the states of the physician and the patient before the beginning of the treatment (correlation coeffi cient 0,18, p>0,05). However, in the process of craniosacral biodynamics, a statistically signifi cant relationship was formed between them with a correlation coeffi cient for non-contact exposure equal to 0,49 (p<0,01) and for contact exposure — 0,34 (p<0,05). Biodynamic treatment statistically signifi cantly improved the state of the autonomic nervous system.Conclusion. The study of the electrical activity of the skin in craniosacral biodynamics is a highly informative method, which allows to verify and quantify the changes occurring in the patient and in the physician during the treatment.

scholarly journals The influence of triple pelvic osteotomy on the spine-pelvis ratios in children with dysplastic subluxation of the hip

2019 ◽  
Vol 7 (2) ◽  
pp. 5-16
Author(s):  
Pavel I. Bortulev ◽  
Sergei V. Vissarionov ◽  
Vladimir E. Baskov ◽  
Dmitriy B. Barsukov ◽  
Ivan Y. Pozdnikin ◽  
...  
Keyword(s):  
Surgical Treatment ◽  
Pelvic Osteotomy ◽  
Main Group ◽  
The State ◽  
Sagittal Profile ◽  
Control Group ◽  
Sacral Slope ◽  
Hip Joints ◽  
X Ray ◽  
Improved Technique

Introduction. Triple pelvic osteotomy is an effective surgical treatment for dysplastic subluxation of the hip in children aged above 12 years. However, modern studies did not assess the indicators of spine-pelvis ratios, sagittal profile of the spine in children with dysplastic subluxation of the hip following surgical treatment, and possibility of change on these values during the operation. Aim. This study aimed to evaluate the effectiveness of the improved technique of performing triple pelvic osteotomy on children with dysplastic subluxation of the hip. Materials and methods. We analyzed the x-ray images and surgical treatment of 35 female patients (44 hip joints) aged 13 to 18 years with dysplastic subluxation of the hip between 2016 and 2018. The patients were divided into two groups: the main group consisted of 20 patients (25 hip joints) who underwent surgical treatment that had taken into account the state of sagittal spine-pelvis ratios according to the improved method using personalized navigation templates, and the control group consisted of 15 patients (19 hip joints) who received surgical treatment according to the generally accepted method. Results. In addition to the typical clinical and radiological abnormalities of the dysplastic subluxation of the hip in 90% of patients in both groups, there were changes in the sagittal balance in the form of excessive pelvic anteversion and lumbar hyperlordosis. One year postoperatively, patients in the main group showed significant changes (p < 0.05) in the state of sagittal spine-pelvis ratios in the form of a decrease in the pelvic base angle, which led to the achievement of the average values of the angle of inclination of the sacral slope (SS) and the value of global lumbar lordosis. On the other hand, these radiological parameters in patients in the control group remained within the preoperative values. Conclusion. The improved technique of triple pelvic osteotomy provides conditions for the reduction of pelvic anteversion and restoration of the sagittal profile of the spine (p < 0.05). The use of personalized navigation templates allows for the most accurate multiplane correction of the acetabulum. It is necessary to include a specialized x-ray examination in the preoperative planning to assess the state of sagittal spine-pelvis ratios.

scholarly journals Evaluation of the state of tubular epithelium in low birth weight infants with ischemic ­nephropathy

2019 ◽  
Vol 100 (6) ◽  
pp. 877-884
Author(s):  
A A Akhundova
Keyword(s):  
Birth Weight ◽  
Low Birth Weight ◽  
Blood Plasma ◽  
Cystatin C ◽  
Main Group ◽  
The State ◽  
Control Group ◽  
Tubular Epithelium ◽  
Low Birth Weight Infants ◽  
Ischemic Nephropathy

Aim. The study of the diagnostic value of KIM-1 and NGAL in urine and cystatin C in the blood plasma of low birth weight infants with ischemic nephropathy. Methods. 150 newborns were divided into 3 groups: main group 72 low birth weight infants with manifestations of ischemic nephropathy divided into three subgroups: group 1A mild (n=36), 1B moderate (n=20), 1C severe (n=16); comparioson group 28 low birth weight infants without the evidence of ischemic nephropathy; control group 50 healthy newborns (20 full-term and 30 preterm).To assess the state of tubular epithelium of the kidneys, the levels of KIM-1 and NGAL were measured in the urine of neonates, to assess the state of glomerular filtration the level of сystatin C in the blood plasma was determined. Samples of blood and urine were collected twice, on days 1 to 3 and 7 to 10. Biomarker levels were determined by solid-phase enzyme immunoassay. Results. In the main group on day 1 to 3 of life KIM-1 and NGAL in the urine were significantly elevated compared to the control group (p 0.001). On day 7 to 10 the level of KIM-1 in the urine in subgroup 1A decreased (0.980.09 ng/dl), while remaining significantly higher compared to the control group, and in subgroups 1B and 1C it increased to 1.240.10 and 1.360.12 ng/dl, respectively. On day 7 to 10 of life the concentration of NGAL in the urine of children of all three subgroups declined, remaining significantly high compared to the control values. Сoncentration of cystatin C was significantly high only in newborns of subgroup 1C (p 0.001). Conclusion. Molecules of NGAL and KIM-1 are early markers of the renal ischemic injury in low birth weight infants who suffered perinatal hypoxia, and cystatin C cannot be considered an early predictor of renal damage in low birth weight infants with ischemic nephropathy as its level in the blood rises only in severe damage.

scholarly journals Application of Fresnel Prism in the Complex Treatment of Friendly Strabismus

2018 ◽  
Vol 15 (2S) ◽  
pp. 98-105
Author(s):  
V. N. Kurochkin ◽  
T. V. Terekhova ◽  
Y. D. Glumskova ◽  
Y. I. Triludina ◽  
O. A. Shelikhova
Keyword(s):  
Surgical Treatment ◽  
Binocular Vision ◽  
Main Group ◽  
The State ◽  
Control Group ◽  
Double Vision ◽  
Complex Treatment ◽  
Good Cosmetic ◽  
Cosmetic Effect ◽  
And Control

Purpose: To analyze the effectiveness of Fresnel prism in the complex treatment of friendly strabismus.Patients and methods. The main group consisted of 428 patients with strabismus, who were assigned Fresnel prisms in combination with pleoptoorthoptic and surgical treatment. Indication for the appointment of Fresnel prism with a friendly strabismus was: angle of strabismus to 25 degrees, postoperative residual angles. The age of the patients ranged from 4 to 60 years. We evaluated the visual acuity without correction and with correction, refraction, character of vision, ability to bifoveal fusion evaluated with synoptophore. The results were compared with a control group treated without Fresnel prisms.Results. During initial selection of Fresnel prisms, doubling was revealed in 73% cases. With the constant wearing of glasses with Fresnel prisms, in which the state of orthotropy is achieved, in most patients doubling was cupped in the period from 2 weeks to 3 months. During the first month, doubling stopped in 72% of patients, and the minimum periods for arresting double vision were noted in preschool children. The absence of doubling served was an indication for surgical correction of strabismus. After the surgery, a good cosmetic effect was achieved in 392 persons (97%), and the state of orthotropy was in 330 of people (81.7%). Doubling after the operation was not observed, there was no relapse of strabismus. Comparative analysis of the main and control groups showed that in the main group of patients the use of prisms combined with pleoptoorthoptic and surgical treatment made it possible to restore binocular vision in 32.9% of cases.Conclusion. The use of Fresnel prism in the complex treatment of friendly strabismus in children allows to obtain not only a good cosmetic effect, but also to develop binocular vision in 32.9% of cases. The use of Fresnel prisms in the preoperative period in adults allows avoiding postoperative double vision, thereby reducing the risk of recurrence of strabismus because of the development of mechanisms for the formation of a single binocular image. 

scholarly journals The state of the local immune status, the content of neopterin and cortisol in different variants of preterm labor

2017 ◽  
Vol 66 (3) ◽  
pp. 60-70
Author(s):  
Nadezhda Yu. Katkova ◽  
Olga I. Bodrikova ◽  
Anzhelika V. Sergeeva ◽  
Larisa D. Andosova ◽  
Ksenia A. Shahova ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Immune Status ◽  
Preterm Labour ◽  
Study Groups ◽  
Main Group ◽  
The State ◽  
Premature Labour ◽  
Control Group ◽  
Term Labour

Premature labour (PL) is one of the most actual and unsolved problem of obstetrics. There are many data in the li terature about the role of the disturbed ratio of pro- and antiinflammatory cytokines, neopterin in the implementation of PL. Many authors support the theory of the predominant fetus factor in the initiation of PL. The goal of our research is studying of the characteristics of the local immune status, neopterin and cortisol levels in various variants of preterm labour. Materials and methods. 77 patients were divided into 2 groups. The main group (52 pregnant women with PL) was also divided into 2 subgroups: the first (1A) subgroup consisted of 28 pregnant women with premature rupture of membranes (PROM) without regular labour contractions. Another subgroup (1B) was presented by 24 women with labour activity and unruptured amniotic sac (true PL). In the control group we included 25 women with term labour. The level of the expression of mRNA genes of a congenital immunity in a cervical canal by the test-system ImmunoQuantex (IL1B, IL10, IL18, TNF2, TLR4, GATA3, CD68, B2M), as well as serum levels of neopterin and cortisol. The results were analyzed by standard statistical methods. Results. There were no significant difference in the expression levels of most of the genes of innate immunity between the study groups (p > 0.05). The investigation of expression of TLR4, GATA3 genes in different types of PL revealed a significant decrease (p < 0.05) in patients with PROM (1A subgroup). In addition in the 1A subgroup the inflammation index was higher (Me = 99,5%, p < 0.01). There were no fundamental difference in the state of the local immune status between groups of true preterm and term labour. Analysis of neopterin content among the subjects showed a significant increase in the main group (PL) compared with the control group (p = 0.0064). The comparison of the neopterin level between different variants of PL had a higher index (p < 0.025). The concentration of cortisol in the study groups had no principle difference from the main (PL) group and control group (p > 0.05). The maximum cortisol level was found in the subgroup 1B (true PL), which is significantly higher than in the subgroup 1A (with PROM) and in the control group (p < 0.01). Conclusion. The difference in the state of local immune status in different variants of premature labour demonstrates different mechanisms of initiation of preterm labour. It is likely that the ratio of TLR4/GATA3 and index of inflammation determins the onset of preterm labour. The level of neopterin can be used as a marker of the onset of PL. The increase of cortisol determines the role of the fetus factor in the onset of labour.

scholarly journals INFLUENCE OF THE TRANSFERRED SYPHILITIC INFECTION ON THE COURSE OF PREGNANCY, CHILDBIRTH AND THE STATE OF NEWBORNS

2019 ◽  
Vol 72 (2) ◽  
pp. 175-180
Author(s):  
Viacheslav M. Husiev ◽  
Daria S. Khapchenkova
Keyword(s):  
Pregnant Women ◽  
Vascular Wall ◽  
Main Group ◽  
The State ◽  
Control Group ◽  
Perinatal Complications ◽  
Pregnancy And Childbirth ◽  
Pathological Conditions ◽  
Intrauterine Hypoxia ◽  
History Of

Introduction: The article presents information about the peculiarities of the course of pregnancy and childbirth in women with a syphilitic infection in the anamnesis. The peculiarities of the state of newborn babies born from mothers who have suffered syphilis are described. To date, the incidence of syphilis in Ukraine has a clear tendency to decline, but still remains quite high. The maximum incidence of syphilis is observed in women aged 15-20 years. The combination of pregnancy and syphilitic infection in an anamnesis is an unfavorable factor in regard to high risk of perinatal complications, the frequency of which does not tend to decrease. The aim - study the features of the course of pregnancy and childbirth in women with a syphilitic infection in the anamnesis, the evaluation of the state of newborns. Materials and methods: A prospective examination of 57 healthy women and their newborns (control group) and 60 pregnant women with a history of syphilitic infection (the main group) had been conducted. All pregnant women had undergone ultrasound examination, including feto- and placentometry, an estimate of the amount of amniotic fluid. The effect of the transferred syphilis on the state of the newborn had been assessed in accordance with the results of the clinical examination of an anthropometric data, including an Apgar score. Results: It is stated that the incidence of latent (41,66%) and forms with a prolonged course (20,00%) of syphilitic infection. The threat of premature childbirth was almost 3,5 times higher than in women with syphilis, cases of an anemia in pregnant women – 2 times, hypertensive disorders of pregnant women were 2,4 times more common in women of the main group, fetal development retardation syndrome 6,4 times, while a greater percentage of this disorder was recorded among women in the main group who were ill with latent forms and suffered secondary recurrent syphilis (35%). In 20% of the cases, pregnancy in women with syphilis has been completed by the cesarean section, an abnormality of the contractile capacity of the uterus was significantly higher – 23,33%. The adaptive capacity of the newborns in the main group has been significantly lower, compared to the control group. Conclusions: Syphilitic infection in the anamnesis complicates the course of pregnancy with numerous pathological conditions. Syphilitic infection, borne before pregnancy, affects not only the course of pregnancy, but also the course of childbirth and the postpartum period. The pathological conditions in infants are due to a decrease in resistance to birth stress, early depletion of adaptive resources of newborns under the influence of a syphilitic infection of the mother. In children who have experienced chronic intrauterine hypoxia, the risk of hemorrhagic syndrome is significantly higher due to increased permeability of the vascular wall. Such children have a tendency to develop neurological disorders and respiratory system lesions.

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