scholarly journals BONE AND SOFT TISSUES INTEGRATION IN POROUS TITANIUM IMPLANTS (EXPERIMENTAL RESEARCH)

2018 ◽  
Vol 24 (2) ◽  
pp. 95-107 ◽  
Author(s):  
R. M. Tikhilov ◽  
I. I. Shubnyakov ◽  
A. O. Denisov ◽  
V. A. Konev ◽  
I. V. Gofman ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Latissimus Dorsi ◽  
Fibrous Tissue ◽  
Porous Titanium ◽  
Additive Technologies ◽  
Titanium Implants ◽  
Knee Joints ◽  
Control Group ◽  
Muscular Tissue ◽  
Tissue Integration

Aim. It’s common that revision arthroplasty of the large joints demands replacing of bone defects of irregular geometrical shapes and simultaneous restoring of support ability and ability to integrate surrounding muscular and tendinous structures into an implant that is required for a complete restoration of joint function.The purpose.To experimentally study the process of integration for muscular and bone tissue as well as tendinous and ligamentous structures into porous titanium materials.Material and methods. During in vivo experiment the authors created a standardized bone defect in 6 rabbits of chinchilla breed at the point of patella ligament attachment as well as a delamination area of muscular tissue in latissimus dorsi. Both knee joints and both latissimus dorsi were used in each animal. Study group included titanium implants with three-dimensional mesh structure. Control group — solid titanium implants with standard porosity. Titanium implants were produced by additive technologies with preliminary prototyping. The porosity corresponded to trabecular metal, striations — 0.45, pores size —100–200 microns. Study and control components were implanted in the identical conditions into the corresponding anatomical sites. Postoperative AP and lateral roentgenograms of knee joints were performed for all animals. Morphological research was conducted on day 60 after the implantation and strength properties were studied at day 90 after the implantation.Results.The authors observed bony ingrowth into implant pores with minimal volume of fibrous tissue, a distinct connective integration was reported represented by a dense fibrous tissue in the pores of components implanted into the muscular tissue. Testing of fixation strength of the study implants demonstrated a clearly superior strength of soft and bone tissue integration into the experimental mesh implants produced using additive technologies.

The experimental study of tissue integration into porous titanium implants

2020 ◽  
pp. 112070002094348
Author(s):  
Rashid Tikhilov ◽  
Igor Shubnyakov ◽  
Alexey Denisov ◽  
Vladimir Konev ◽  
Iosif Gofman ◽  
...  
Keyword(s):  
Tensile Strength ◽  
Bone Defect ◽  
Porous Titanium ◽  
Titanium Implants ◽  
Control Group ◽  
Additive Technology ◽  
Tissue Integration ◽  
3D Printed ◽  
Tissue Attachment ◽  
Highly Porous

Introduction: Due to a lack of uniform shapes and sizes of bone defects in hip and knee joint pathology, their fixing could benefit from using individually manufactured 3D-printed highly porous titanium implants. The objective of this study was to evaluate the extent of bone and muscle tissue integration into porous titanium implants manufactured using additive technology. Materials and methods: Porous and non-porous titanium plates were implanted into the latissimus dorsi muscle and tibia of 9 rabbits. On days 1, 60 and 90 animals were examined with x-rays. On day 60 histological tests were carried out. On day 90 the tensile strength at the implant-tissue interface was tested. Results: Histological analysis of muscle samples with porous titanium implants showed integration of connective tissue and blood vessels into the pores. Bone defect analysis demonstrated bone ingrowth into the pores of titanium with a minimal amount of fibrous tissue. The tensile strength of the muscular tissue attachment to the porous titanium was 28 (22–30) N which was higher than that of the control group 8.5 (5–11) N. Bone tissue attachment strength was 148 (140–152) N in the experimental group versus 118 (84–122) N in the control group. Conclusions: Using additive technology in manufacturing 3D-printed highly porous titanium implants improves bone and muscle integration compared with the non-porous material of the control group. This could be a promising approach to bone defect repair in revision and reconstruction surgery.

scholarly journals Hydroxyapatite-lignin composite as a metallic implant-bone tissue osseointegration improver: experimental study in dogs

2015 ◽  
Vol 46 (2) ◽  
pp. 324-329 ◽  
Author(s):  
Fabrício Luciani Valente ◽  
Letícia Corrêa Santos ◽  
Rodrigo Viana Sepúlveda ◽  
Gustavo Pereira Gonçalves ◽  
Renato Barros Eleotério ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Statistical Difference ◽  
Histological Analysis ◽  
Control Group ◽  
X Ray Diffraction ◽  
Metal Implant ◽  
X Ray ◽  
Marrow Cavity ◽  
Biomechanical Assessment ◽  
Tissue Integration

The study aimed to evaluate biocompatibility, osteoconduction and osseointegration of a pasty composite of hydroxyapatite (20%) and lignin (80%) as a promoter of metal implant and bone tissue integration. An intramedullary Schanz pin was implanted in both tibias of fifteen bitches. In the left tibia, the pin was coated with the biomaterial at the time of surgery. Marrow cavity was also filled with the biomaterial. Right limb did not receive the biomaterial, then constituting the control group. Tibias were harvested from five animals at 8, 60 and 150 days after surgery; three of them were analyzed by histological and biomechanical assessment and the two remaining tibias by X-ray diffraction. Results showed that the biomaterial is biocompatible, with osteoconductivity and osseointegration properties. Histological analysis and diffractograms showed the presence of hydroxyapatite in samples in all periods, although the presence of organic material of low crystallinity was variable. There was no statistical difference in the forces required for removal of the biocompatibility, osteoconductivity and osseointegration, it was not able to promote a better intramedullary pin anchorage.

A porous bioactive titanium implant for spinal interbody fusion: an experimental study using a canine model

2007 ◽  
Vol 7 (4) ◽  
pp. 435-443 ◽  
Author(s):  
Mitsuru Takemoto ◽  
Shunsuke Fujibayashi ◽  
Masashi Neo ◽  
Kazutaka So ◽  
Norihiro Akiyama ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Histological Examination ◽  
Interbody Fusion ◽  
Average Pore Size ◽  
Spinous Process ◽  
Fibrous Tissue ◽  
Porous Titanium ◽  
Titanium Implants ◽  
Average Pore ◽  
New Generation

Object Porous biomaterials with adequate pore structure and appropriate mechanical properties are expected to provide a new generation of devices for spinal interbody fusion because of their potential to eliminate bone grafting. The purpose of this study was to evaluate the fusion characteristics of porous bioactive titanium implants using a canine anterior interbody fusion model. Methods Porous titanium implants sintered with volatile spacer particles (porosity 50%, average pore size 303 μm, compressive strength 116.3 MPa) were subjected to chemical and thermal treatments that give a bioactive microporous titania layer on the titanium surface (BT implant). Ten adult female beagle dogs underwent anterior lumbar interbody fusion at L6–7 using either BT implants or nontreated (NT) implants, followed by posterior spinous process wiring and facet screw fixation. Radiographic evaluations were performed at 1, 2, and 3 months postoperatively using X-ray fluoroscopy. Animals were killed 3 months postoperatively, and fusion status was evaluated by manual palpation and histological examination. Results Interbody fusion was confirmed in all five dogs in the BT group and three of five dogs in the NT group. Histological examination demonstrated a large amount of new bone formation with marrowlike tissue in the BT implants and primarily fibrous tissue formation in the NT implants. Conclusions Bioactive treatment effectively enhanced the fusion ability of the porous titanium implants. These findings, coupled with the appropriate mechanical properties in load-bearing conditions, indicate that these porous bioactive titanium implants represent a new generation of biomaterial for spinal interbody fusion.

Effects of pore size and porosity of surface-modified porous titanium implants on bone tissue ingrowth

2019 ◽  
Vol 29 (12) ◽  
pp. 2534-2545 ◽  
Author(s):  
Jing-pu ZHENG ◽  
Liang-jian CHEN ◽  
Dai-yuan CHEN ◽  
Chun-sheng SHAO ◽  
Man-fei YI ◽  
...  
Keyword(s):  
Pore Size ◽  
Bone Tissue ◽  
Porous Titanium ◽  
Titanium Implants ◽  
Tissue Ingrowth ◽  
Surface Modified

Dual modulation of crystallinity and macro-/microstructures of 3D printed porous titanium implants to enhance stability and osseointegration

2019 ◽  
Vol 7 (17) ◽  
pp. 2865-2877 ◽  
Author(s):  
Ping Song ◽  
Cheng Hu ◽  
Xuan Pei ◽  
Jianxun Sun ◽  
Huan Sun ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Porous Titanium ◽  
Titanium Implants ◽  
Bone Tissue Regeneration ◽  
3D Printed

The macro architecture and micro surface topological morphology of implants play essential roles in bone tissue regeneration.

scholarly journals The use of fine - grained nickelide titanium, in the experiment, for the pathology of the musculoskeletal system.

2019 ◽  
Vol 12 (2) ◽  
pp. 107-110
Author(s):  
Pavel Viktorovich Trushin ◽  
Vladimir Andreevich Golovnev ◽  
Sergey Grigorevich Shtofin
Keyword(s):  
Bone Tissue ◽  
Traditional Method ◽  
Titanium Nickelide ◽  
Porous Titanium ◽  
Control Group ◽  
Fine Grained ◽  
Fine Grain ◽  
Regeneration Of Bone Tissue ◽  
Regeneration Of Bone ◽  
Porous Titanium Nickelide

The aim is to study and compare the processes of regeneration of bone tissue in animals while grafting titanium nickelide granules in bone cavities and free plastics in experiment. Methods. Two methods of bone grafting were experimentally reproduced to assess the speed of regeneration of bone tissue of dogs’. The first is traditional method with bone crumbs, and the second one of filling the bone cavity with fine-grain titanium nickelide. The last-named has been developed lately. In the first group consisting of 25 dogs, granules of porous titanium nickelide were placed in the formed cavity of shin bone epiphysis. In the second (control) group (consisting of 10 dogs), bone crumbs were introduced into the cavity of the shin bone. Results. The experiment showed that fine-grain titanium nickelide use for grafting bone cavity in comparison with bone crumbs demonstrated the formation of bone organo-typical regenerate 3 months faster. Conclusions. Fine-grain titanium nickelide is a promising material and can be used in the pathology of the skeleton.

scholarly journals Complete Osseointegration of a Retrieved 3-D Printed Porous Titanium Cervical Cage

2020 ◽  
Vol 7 ◽  
Author(s):  
Wimar van den Brink ◽  
Nancy Lamerigts
Keyword(s):  
Animal Studies ◽  
Bone Ingrowth ◽  
Fibrous Tissue ◽  
Case Series ◽  
Stress Shielding ◽  
Porous Titanium ◽  
Titanium Implants ◽  
Histological Evaluation ◽  
Anterior Plate ◽  
Bone Apposition

Introduction: Porous 3D-printed titanium has only recently been introduced for spinal applications. Evidence around its use is currently limited to animal studies and only few human case series. This study describes the histological findings of a retrieved EIT cervical cage, explanted 2 years after insertion.Materials and Methods: The patient underwent a double level C4/C5 & C5/C6 anterior cervical decompression using EIT cervical cages without an anterior plate. Two years later the C6/7 level degenerated and began to cause myelopathic symptoms. In order to address the kyphotic imbalance of the cervical spine and fix the C6/7 level, the surgeon decided to remove the C5/6 cervical cage and bridge the fusion from C4 to C7 inclusive. The retrieved cage was histologically evaluated for bone ingrowth and signs of inflammation.Results: MRI demonstrated spinal canal stenosis at C6/C7. Plain radiographs confirmed well-integrated cervical cages at 2 years postoperative. The peroperative surgical need to use a chisel to remove the implant at C5/C6 reconfirmed the solid fusion of the segment. Macroscopically white tissue, indicative of bone, was present at both superior and inferior surfaces of the explanted specimen. Histological evaluation revealed complete osseointegration of the 5 mm high EIT Cellular Titanium® cervical cage, displaying mature lamellar bone in combination with bone marrow throughout the cage. Furthermore, a pattern of trabecular bone apposition (without fibrous tissue interface) and physiological remodeling activity was observed directly on the cellular titanium scaffold.Conclusion: This histological retrieval study of a radiologically fused cervical EIT cage clearly demonstrates complete osseointegration within a 2-year time frame. The scaffold exhibits a bone in growth pattern and maturation of bone tissue similar of what has been demonstrated in animal studies evaluating similar porous titanium implants. The complete osseointegration throughout the cage indicates physiological loading conditions even in the central part of the cage. This pattern suggests the absence, or at least the minimization, of stress-shielding in this type of porous titanium cage.

scholarly journals Genes associated with inflammation and bone remodeling are highly expressed in the bone of patients with the early-stage cam-type femoroacetabular impingement

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Guanying Gao ◽  
Ruiqi Wu ◽  
Rongge Liu ◽  
Jianquan Wang ◽  
Yingfang Ao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Tissue ◽  
Bone Remodeling ◽  
Femoroacetabular Impingement ◽  
Early Stage ◽  
Control Group ◽  
Tissue Samples ◽  
Expression Levels ◽  
Normal Bone ◽  
Impingement Zone

Abstract Background Recent studies have shown high expression levels of certain inflammatory, anabolic, and catabolic genes in the articular cartilage from the impingement zone of the hips with femoroacetabular impingement (FAI), representing an increased metabolic state. Nevertheless, little is known about the molecular properties of bone tissue from the impingement zone of hips with FAI. Methods Bone tissue samples from patients with early-stage cam-type FAI were collected during hip arthroscopy for treatment of cam-type FAI. Control bone tissue samples were collected from six patients who underwent total hip replacement because of a femoral neck fracture. Quantitative real-time polymerase chain reaction (PCR) was performed to determine the gene expression associated with inflammation and bone remodeling. The differences in the gene expression in bone tissues from the patients with early-stage cam-type FAI were also evaluated based on clinical parameters. Results In all, 12 patients with early-stage cam-type FAI and six patients in the control group were included in this study. Compared to the control samples, the bone tissue samples from patients with FAI showed higher expression levels of interleukin-6 (IL-6), alkaline phosphatase (ALP), receptor activator of nuclear factor-kB ligand (RANKL), and osteoprotegerin (OPG) (P < 0.05). IL-1 expression was detected only in the control group. On the other hand, there was no significant difference in IL-8 expression between the patients with FAI and the control group. The patients with FAI having a body mass index (BMI) of >24 kg/m2 showed higher ALP expression (P < 0.05). Further, the expression of IL-6 and ALP was higher in the patients with FAI in whom the lateral center-edge angle was >30° (P < 0.05). Conclusions Our results indicated the metabolic condition of bone tissues in patients with early-stage cam-type FAI differed from that of normal bone in the femoral head-neck junction. The expression levels of the genes associated with inflammation and bone remodeling were higher in the bone tissue of patients with early-stage cam-type FAI than in the patients with normal bone tissue.

Evaluation of electroacupuncture for symptom modification in a rodent model of spontaneous osteoarthritis

2021 ◽  
pp. 096452842110207
Author(s):  
Alexa P Spittler ◽  
Maryam F Afzali ◽  
Richard B Martinez ◽  
Lauren A Culver ◽  
Sarah E Leavell ◽  
...  
Keyword(s):  
Rodent Model ◽  
Knee Joints ◽  
Control Group ◽  
Disease Modification ◽  
Inflammatory Biomarker ◽  
Knee Oa ◽  
Significant Difference ◽  
Gene Transcripts ◽  
Restricted Mobility ◽  
Movement Parameters

Objective: Faced with the frustration of chronic discomfort and restricted mobility due to osteoarthritis (OA), many individuals have turned to acupuncture for relief. However, the efficacy of acupuncture for OA is uncertain, as much of the evidence is inconclusive. The purpose of this study was to evaluate electroacupuncture (EA) in a rodent model of OA such that conclusions regarding its effectiveness for symptom or disease modification could be drawn. Methods: Ten 12-month-old male Hartley guinea pigs—which characteristically have moderate to advanced OA at this age—were randomly assigned to receive EA for knee OA (n = 5) or anesthesia only (control group, n = 5). Treatments were performed three times weekly for 3 weeks, followed by euthanasia 2 weeks later. Gait analysis and enclosure monitoring were performed weekly to evaluate changes in movement. Serum was collected for inflammatory biomarker testing. Knee joints were collected for histology and gene expression. Results: Animals receiving EA had significantly greater changes in movement parameters compared to those receiving anesthesia only. There was a tendency toward decreased serum protein concentrations of complement component 3 (C3) in the EA group compared to the control group. Structural and antioxidant gene transcripts in articular cartilage were increased by EA. There was no significant difference in total joint histology scores between groups. Conclusion: This study provides evidence that EA has a positive effect on symptom, but not disease, modification in a rodent model of OA. Further investigations into mechanistic pathways that may explain the efficacy of EA in this animal model are needed.

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