Hydroxyapatite-lignin composite as a metallic implant-bone tissue osseointegration improver: experimental study in dogs

The study aimed to evaluate biocompatibility, osteoconduction and osseointegration of a pasty composite of hydroxyapatite (20%) and lignin (80%) as a promoter of metal implant and bone tissue integration. An intramedullary Schanz pin was implanted in both tibias of fifteen bitches. In the left tibia, the pin was coated with the biomaterial at the time of surgery. Marrow cavity was also filled with the biomaterial. Right limb did not receive the biomaterial, then constituting the control group. Tibias were harvested from five animals at 8, 60 and 150 days after surgery; three of them were analyzed by histological and biomechanical assessment and the two remaining tibias by X-ray diffraction. Results showed that the biomaterial is biocompatible, with osteoconductivity and osseointegration properties. Histological analysis and diffractograms showed the presence of hydroxyapatite in samples in all periods, although the presence of organic material of low crystallinity was variable. There was no statistical difference in the forces required for removal of the biocompatibility, osteoconductivity and osseointegration, it was not able to promote a better intramedullary pin anchorage.

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Histological and X-ray structural analysis of the conditionof the bone tissue of the jaws with direct and delayed implantation using implants with different surface microstructures

SUCHASNA STOMATOLOHIYA ◽

10.33295/1992-576x-2021-4-24 ◽

2021 ◽

Vol 108 (4) ◽

pp. 24-32

Author(s):

P. Grishin ◽

◽

E. Mamaeva ◽

E. Kalinnikova ◽

E. Kushner ◽

...

Keyword(s):

Structural Analysis ◽

Key Words ◽

Bone Tissue ◽

Animal Experiment ◽

Histological Analysis ◽

X Ray Diffraction ◽

X Ray ◽

Delayed Implantation ◽

Surface Microstructures ◽

Bone Condition

Abstract. This article presents the results of histological and X-ray studies of bone condition, stability, and degree of osteointegration of implants with different microstructure of the surface during immediate and delayed implantation in the animal experiment. The results of the study did not reveal significant differences in the process of osteointegration during direct and delayed implantation. Data from histological and X-ray studies of direct implantation in fresh extraction holes are comparable to those obtained during delayed implantation and are not inferior to the traditional two-stage protocol. At the same time, a certain correlation between the type of surface of the implant and the time of its adaptation in different periods of the experiment was revealed. When using an implant with an innovative surface, HSTTM. The process of osteointegration is mor clear, and the bone wall of the hole is more compacted, which indicates an accelerated and successful process of osteointegration. Key words: X-ray diffraction, frequency resonance and histological analysis, fibrous capsule, collagen fibers, trabeculae, bone tissue, periotestometry osseointegration, stability.

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Evaluating the Toxicity and Histological Effects of Al2O3 Nanoparticles on Bone Tissue in Animal Model: A Case-Control Study

Journal of Toxicology ◽

10.1155/2020/8870530 ◽

2020 ◽

Vol 2020 ◽

pp. 1-8

Author(s):

Hossein Soltaninejad ◽

Hadi Zare-Zardini ◽

Amir Ali Hamidieh ◽

Mohammad Reza Sobhan ◽

Seyed Houssein Saeed-Banadaky ◽

...

Keyword(s):

Bone Density ◽

Bone Tissue ◽

Surgical Instruments ◽

Control Group ◽

Average Particle ◽

Al2o3 Nanoparticles ◽

X Ray Diffraction ◽

Male Adult ◽

X Ray ◽

Al2o3 Nanoparticle

The applications of nanostructures have been limited by their different toxicities. So, the investigation of these toxicities is necessary before nanostructure application. This study aimed to evaluate the effect of aluminum oxide (Al2O3) nanoparticles on bone density in Wistar rat. Al2O3 nanoparticle was prepared by the sol-gel method. Characterization was done by X-ray diffraction (XRD) and transmission electron microscopy (TEM). Sixty-four male adult Wistar rats were divided into eight groups including six groups intravenously treated with Al2O3 nanoparticle at concentrations of 25, 50, 100, 250, 500, and 1000 µg/ml: one group received food and water as the control group, and one group received food and water as well as intravenously distilled water as an injection control group. After 41 days, bone density was analyzed by dual-energy X-ray absorptiometry (DEXA). According to X-ray diffraction, the average particle size for Al2O3 nanoparticles was 20.85 nm. The data of densitometry showed that the bone density of right and left foot was reduced in concentrations of 250, 500, and 1000 µg/ml that were statistically significant in comparison with the control group. The reduction of bone density was increased with the enhancement of nanostructures concentration. The effect of Al2O3 nanoparticles on bone density was similar in the left and right legs. Histopatholological assessment also showed that Al2O3 nanoparticles (250, 500, and 1000 µg/ml) lead to significant reduction of trabeculae. Empty lacunae are observed in these three groups. Considering that high concentrations of Al2O3 nanoparticles had toxicity on bone tissue, it must be used by more caution, especially its use as a coating in different devices such as implants, surgical instruments, and bone prostheses.

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Effect of the suspension of Ag-incorporated TiO2 nanoparticles (Ag-TiO2 NPs) on certain growth, physiology and phytotoxicity parameters in spinach seedlings

PLoS ONE ◽

10.1371/journal.pone.0244511 ◽

2020 ◽

Vol 15 (12) ◽

pp. e0244511

Author(s):

Fernando Gordillo-Delgado ◽

Jakeline Zuluaga-Acosta ◽

Gonzalo Restrepo-Guerrero

Keyword(s):

Particle Size ◽

Titanium Dioxide Nanoparticles ◽

Germination Rate ◽

Sol Gel ◽

Control Group ◽

Human Consumption ◽

X Ray Diffraction ◽

X Ray ◽

Growth Physiology ◽

Powder Samples

In this work, the effect of the inoculation of silver-incorporated titanium dioxide nanoparticles (Ag-TiO2 NPs) in spinach seeds was evaluated on certain growth, physiology and phytotoxicity parameters of the plants. This is an important crop for human consumption with high nutritional value due to their low calorie and fat content, providing various vitamins and minerals, especially iron. These NPs were obtained by means of the sol-gel method and heat treatment; the resulting powder material was characterized using X-ray diffraction and scanning electron microscopy and the influence of these NPs on plants was measured by estimating the germination rate, monitoring morphological parameters and evaluating phytotoxicity. The photosynthetic activity of the spinach plants was estimated through the quantification of the Ratio of Oxygen Evolution (ROE) by the photoacoustic technique. Samples of TiO2 powder with particle size between 9 and 43 nm were used to quantify the germination rate, which served to determine a narrower size range between 7 and 26 nm in the experiments with Ag-TiO2 NPs; the presence of Ag in TiO2 powder samples was confirmed by energy-dispersive X-ray spectroscopy. The analysis of variance showed that the dependent variable (plant growth) could be affected by the evaluated factors (concentration and size) with significant differences. The statistical trend indicated that the application of the Ag-TiO2 NPs suspension of lowest concentration and smallest particle size could be a promoting agent of the growth and development of these plants. The inoculation with NPs of 8.3 nm size and lowest concentration was related to the highest average ROE value, 24.6 ± 0.2%, while the control group was 20.2 ± 0.2%. The positive effect of the Ag-TiO2 NPs treatment could be associated to the generation of reactive oxygen species, antimicrobial activity, increased biochemical attributes, enzymatic activity or improvements in water absorption.

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Bioactive Composite Materials for Bone Tissue Applications

Materials Science Forum ◽

10.4028/www.scientific.net/msf.514-516.985 ◽

2006 ◽

Vol 514-516 ◽

pp. 985-989

Author(s):

B.J.M. Leite Ferreira ◽

M.G.G.M. Duarte ◽

M. Helena Gil ◽

Rui N. Correia ◽

J. Román ◽

...

Keyword(s):

Electron Microscopy ◽

Bone Tissue ◽

Tissue Repair ◽

Body Fluid ◽

In Vitro Bioactivity ◽

X Ray Diffraction ◽

X Ray ◽

Biodegradable Composite ◽

Scanning Electron

Two materials with potential application in bone tissue repair have been developed: 1) a non-biodegradable composite based in a new methacrylic-co-acrylic matrix; and 2) a biodegradable composite based in a chitosan (Ch) matrix. Both matrices were reinforced with glass-ceramic particles of composition (mol%) 70 SiO2 – 30 CaO. The in vitro bioactivity of composites was assessed by soaking in simulated body fluid (SBF) for periods of up to 7 days at 37º C. X-ray diffraction (XRD) and scanning electron microscopy coupled with X-ray energy dispersive spectroscopy (SEM-EDS) were used for deposit identification after different soaking periods. Calcium phosphate particulate deposits were detected after 3 days of immersion, followed by growth and maturation towards apatite.

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Lattice Strain of HAp in Bone Tissue using X-ray Diffraction Method

The proceedings of the JSME annual meeting ◽

10.1299/jsmemecjo.2002.6.0_39 ◽

2002 ◽

Vol 2002.6 (0) ◽

pp. 39-40

Author(s):

Kazuhiro FUJISAKI ◽

Shigeru TADANO ◽

Junichi SHIBANO

Keyword(s):

Bone Tissue ◽

Lattice Strain ◽

Diffraction Method ◽

X Ray Diffraction ◽

X Ray

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Hydroxyapatite/Biopolymers Composite Scaffolds for Bone Tissue Engineering

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.493-494.826 ◽

2011 ◽

Vol 493-494 ◽

pp. 826-831

Author(s):

A.C.B.M. Fook ◽

Thiago Bizerra Fideles ◽

R.C. Barbosa ◽

G.T.F.S. Furtado ◽

G.Y.H. Sampaio ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Freeze Drying ◽

Three Dimensional ◽

Porous Scaffolds ◽

X Ray Diffraction ◽

X Ray ◽

Interconnected Pores

The application of a hybrid composite consisting of biopolymer and calcium phosphate, similar morphology and properties of natural bone, may be a way to solve the problem of the fragility of ceramics without reducing its mechanical properties, retaining the properties of biocompatibility and high bioactivity. This work aims at the preparation and characterization of three-dimensional scaffolds composite HA / biopolymers (chitosan and gelatin). The freeze-drying technique was employed in this study to obtain these frameworks and partial results showed the effectiveness of this method. This involved the study of structural, chemical and morphological frameworks, in order to direct the research suggested the application. The X Ray Diffraction (XRD) and infrared spectroscopy and Fourier transform (FTIR) results confirmed the formation of hydroxyapatite (HA) phase and the presence of characteristic bands of HA and biopolymers in all compositions. The microstructure of the scaffolds study conducted by Scanning Electron Microscopy (SEM) revealed the formation of longitudinally oriented microchannels with interconnected pores. In all compositions the porous scaffolds showed varying sizes and mostly larger than 100μm, and is therefore considered materials with potential for application in bone tissue engineering.

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Glass Ionomer Cement – Development and Characterization Microstructural

Materials Science Forum ◽

10.4028/www.scientific.net/msf.805.12 ◽

2014 ◽

Vol 805 ◽

pp. 12-18

Author(s):

Waldênia P. Freire ◽

Marcus Vinícius Lia Fook ◽

Emilly F. Barbosa ◽

Camila S. Araújo ◽

Rossemberg C. Barbosa ◽

...

Keyword(s):

Glass Ionomer Cement ◽

Homogeneous Distribution ◽

Control Group ◽

Bone Cements ◽

Glass Ionomer ◽

X Ray Diffraction ◽

X Ray ◽

Luting Agent ◽

Diffraction Patterns ◽

Ionomer Cement

The Glass Ionomers Cements (GICs) are materials widely used in dentistry, have advantages such as fluoride release and chemical adhesion to the dental substrate. They are recommended as a restorative material, luting agent in prosthetic dentistry and also in medicine. However, there is need for developing new bone cements as an alternative or replacement to the current polymethylmethacrylate cements, therefore, the objective of this research was to develop an experimental GIC and characterization regarding morphology, chemical composition and crystallinity. This composite was characterized by X-ray diffraction (XRD), Infrared Spectroscopy Fourier Transform (FTIR) and optical microscopy (OM). For comparative study, was used the GIC Vidrion R (SS White) in the control group. These cements are presented in semi-crystalline diffraction patterns, the FTIR spectra observed characteristic bands of these materials and microstructural study of the cements showed homogeneous distribution of filler in the polymer matrix, corroborating with the literature.

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Levels of C-terminal propeptide of type I procollagen and oxyproline in patients with systemic lupus erythematosus: The relationship between the course of the disease and the structural and functional state of bone tissue

Regulatory Mechanisms in Biosystems ◽

10.15421/021718 ◽

2017 ◽

Vol 8 (2) ◽

pp. 105-110

Author(s):

S V. Shevchuk ◽

L. P. Denyschych ◽

L. I. Marynych

Keyword(s):

Systemic Lupus Erythematosus ◽

Bone Resorption ◽

Bone Metabolism ◽

Bone Tissue ◽

Lupus Erythematosus ◽

Control Group ◽

Type I ◽

Systemic Lupus ◽

X Ray ◽

Type I Procollagen

The high prevalence of osteoporosis in patients with systemic lupus erythematosus (SLE) makes it necessary to study the abnormalities in bone metabolism, its relationship with reduced bone mineral density (BMD) and the impact of the disease on it. The aim of this study was to determine serum levels of C-terminal propeptide of type I procollagen (PICP) and oxyproline in patients with SLE, their comparison with the structural and functional state of the patients’ bone tissue and the course of the disease. We examined 58 female SLE patients. The mean age of the patients was 45.1 ± 1.0 years. The control group included 29 healthy individuals,corresponding in age and sex with the researched group. For every patient, data were recorded on age, body mass index (BMI), menstrual history, smoking, chronic SLE damage (SLICC/ACR DI) and disease activity score (SLEDAI), cumulative glucocorticoid dose, serum concentrations of interleukin-6 (IL-6) and C-reactive protein (CRP), bone formation marker (C-terminal propeptide of type I procollagen) and bone resorption marker (oxyproline). In all patients BMD was measured by DXA (Dual-energy X-ray absorptiometry) at two sites. To determine vertebral compression fractures, female SLE patients were examined with an x-ray of the thoracic and lumbar spine. We established that bone turnover markers showed a significant difference between the SLE patients and the control group, with lower levels of PICP and higher levels of oxyproline in the SLE patients. Alterations of bone metabolism were associated with the severity of the disease, active inflammation (high levels of CRP and IL-6), the age of the patients, and the high cumulative glucocorticoid dose but no correlation was found with disease duration, BMI and smoking. Patients with osteopenia, osteoporosis and fractures were significantly more frequently found among patients with reduced bone formation and increased bone resorption rate. Thus, our findings showed that female SLE patients have alterations of bone metabolism in the form of increasing serum oxyproline and reducing serum C-terminal propeptide of type I procollagen, the correction of which would slow the progression of adverse structural and functional changes in the bone tissue.

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Effects of coating Populus nigra wood with nanoclay

BioResources ◽

10.15376/biores.15.4.8026-8038 ◽

2020 ◽

Vol 15 (4) ◽

pp. 8026-8038

Author(s):

Mostafa Emampour ◽

Habibollah Khademieslam ◽

Mohammad Mehdi Faezipour ◽

Mohammad Talaeipour

Keyword(s):

Contact Angle ◽

Water Absorption ◽

Abies Alba ◽

Dip Coating ◽

Populus Nigra ◽

Control Group ◽

Performance Parameters ◽

X Ray Diffraction ◽

X Ray ◽

Flexural Resistance

This study evaluated the effects of coating Populus nigra wood with nanoclay by dip-coating and spin-coating. The samples were coated with nanoclay in concentrations of 3, 5, and 7%, and the products were compared with Abies alba wood as standard control for the morphological and moisture properties. Zycosil was also added into nanoclay as an adaptive material. The crystalline and morphological structures were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM). Performance parameters including water absorption, contact angle, density, and flexural resistance were investigated. Water absorption was decreased, but the density, flexural resistance, and contact angle were increased in nanoclay treatments compared with the P. nigra control group (P<0.05). The best performance properties were observed at a concentration of 7%. In sum, the concentration of 7% nanoclay in dip-coating decreased water absorption by 250% and increased water angle by 150%, density by 113%, and flexural resistance by 145% compared to P. nigra control group.

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Optimization of Hydroxyapatite Synthesis and Microplasma Spraying of Porous Coatings Onto Titanium Implants

Advances in Materials Science ◽

10.1515/adms-2017-0043 ◽

2018 ◽

Vol 18 (3) ◽

pp. 79-94 ◽

Cited By ~ 3

Author(s):

D. L. Alontseva ◽

M. B. Abilev ◽

A. M. Zhilkashinova ◽

S. G. Voinarovych ◽

O. N. Kyslytsia ◽

...

Keyword(s):

Titanium Alloy ◽

Diffraction Analysis ◽

Bone Tissue ◽

Titanium Implants ◽

Titanium Coating ◽

X Ray Diffraction ◽

Implant Surface ◽

Porosity Level ◽

X Ray ◽

Biocompatible Coatings

AbstractThe paper presents the main results of development and optimization of the synthesis of hydroxyapatite and the application of the micro-plasma spraying technique for biocompatible coatings. The hydroxyapatite synthesis was optimized using the mathematical modelling method. Synthesized hydroxyapatite was studied by IR spectrometry and X-ray diffraction analysis for assessment of the compatibility of the chemical and phase composition to the bone tissue. The Ca/P ratio of the obtained hydroxyapatite was 1.65, which is close to that of bone tissue (1.67). To increase the adhesion strength of the HA coating to the surface of the titanium implant, it was suggested to apply a titanium sublayer to the implant surface. Microplasma spraying (MPS) of biocompatible coatings from titanium wires and synthesized HA powders onto substrates made of medical titanium alloy has been carried out. Microplasmatron MPN-004 is used to obtain the two-layer coatings for titanium implants. The two layer coating includes a sub-layer of a porous titanium coating with a thickness in range from 200 up to 300 μm and the porosity level of about 30%, and an upper layer of HA about 100 μm thick with 95% level of HA phases and 93% level of crystallinity. The pore size varies from 20 to 100 μm in both coatings. The paper describes the technology and modes of microplasma deposition of two-layer coatings, including the mode of gas-abrasive treatment of the surface of implants made of titanium alloy before spraying. The synthesized HA powder and the Ti/HA coatings were investigated by optical microscopy and scanning electron microscopy with the energy dispersion analysis and the X-ray diffraction analysis.

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