scholarly journals Use of corticosteroid therapy on the modulation of uterine inflammatory response in mares after artificial insemination with frozen semen

2008 ◽  
Vol 24 (1) ◽  
pp. 79-82 ◽  
Author(s):  
F O Papa ◽  
J A DellAqua Jr ◽  
M A Alvarengo ◽  
C Marini Melo ◽  
F Soares Zahn ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Corticosteroid Therapy ◽  
Artificial Insemination ◽  
Frozen Semen

scholarly journals Comparative study of semen quality traits between Etawah grade and Senduro bucks

2021 ◽  
Vol 888 (1) ◽  
pp. 012022
Author(s):  
N Isnaini ◽  
A A Hakim ◽  
D Amertaningtyas ◽  
H E Sulistyo ◽  
A Irsyammawati ◽  
...  
Keyword(s):  
Comparative Study ◽  
Artificial Insemination ◽  
Recovery Rate ◽  
Semen Quality ◽  
T Test ◽  
Quality Traits ◽  
Spermatozoa Motility ◽  
Frozen Semen ◽  
Semen Volume

Abstract This study compared the semen quality traits between Etawah grade (EGB) and Senduro bucks (SB) raised in Singosari National Artificial Insemination Center, Indonesia. A total of 96 ejaculated semen were collected from three EGB and three SB with an average age of three years. The ejaculates were evaluated for semen volume (SV), spermatozoa concentration (SC), total spermatozoa (TS), spermatozoa motility (SM), post-thawing spermatozoa motility (PTSM), the recovery rate of spermatozoa motility (RRSM), and frozen semen production (FSP). Data of SV, SM, PTSM, and RRSM were compared using the Mann-Whitney U test. While the data of SC, TS, and FSP were analyzed using an independent t-test. The results showed that SV, SM, PTSM, and RRSM were not significantly differed (P>0.05) between breeds. Whereas, SC, TS, and FSP of EGB were significantly higher (P<0.05) than SB. Therefore, it can be concluded that Etawah grade bucks can produce higher total spermatozoa and as a result higher frozen semen production for artificial insemination as compared to Senduro bucks.

scholarly journals Viability of Peranakan Etawah Liquid Semen Preserved in Tris Substituted with Various Energy Sources

2020 ◽  
Vol 25 (2) ◽  
pp. 68
Author(s):  
Nurul Afzan Hilda Zakiya ◽  
A H Yanti ◽  
T R Setyawati
Keyword(s):  
Energy Source ◽  
Artificial Insemination ◽  
Block Design ◽  
Egg Yolk ◽  
Energy Sources ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Semen Preservation ◽  
Semen Extender ◽  
Fresh Semen

The use of liquid semen for artificial insemination program of Etawah crossbreed goat (PE) is an alternative to replace frozen semen which is constrained by limited and expensive facilities. Production of liquid semen is faster than frozen semen, but the viability of liquid semen which preserved with a standard extender such as tris egg yolk is very short. The purpose of this study was to determine the viability of PE goat semen in egg yolk tris substituted with energy sources such as glucose, galactose, and mannose and to determine the most efficient energy source for semen preservation. This research was conducted from August to September 2018 at the Artificial Insemination Center in Lembang, West Java. This study was designed in a randomized block design (RBD) consist of three experimental groups divided into five groups. Fresh semen of PE goats were preserved using extender which energy source has been modified. Results showed that using glucose in PE goat semen extender produced the best motility among other groups (64.29 ± 9.2%). The highest viability was found in extender with fructose substitution (86.76 ± 2.3%). The longest viability of liquid semen was found in the extender with glucose substitution. It lasted for six days.

scholarly journals Repeatability and Phenotypic Correlation Among Semen Quality Traits in Holstein Bulls

2018 ◽  
Vol 42 (4) ◽  
Author(s):  
Argi Argiris ◽  
Siswanto Imam Santoso ◽  
Yon Supri Ondho ◽  
Edy Kurnianto
Keyword(s):  
Artificial Insemination ◽  
Semen Quality ◽  
Intraclass Correlation ◽  
Correlation Method ◽  
Phenotypic Correlation ◽  
Quality Traits ◽  
Frozen Semen ◽  
Holstein Bulls ◽  
Fresh Semen ◽  
Selection Of

The purpose of this research was to analysis the value of repeatability and correlation among the traits affecting the production of frozen semen from Holstein’s bull in Indonesia. Repeatability and correlation were calculated based on the data of frozen semen production of 15.699 records from 44 Holstein bulls at Singosari Artificial Insemination Center (SAIC) and 8.935 records from 39 Holstein bulls at Lembang Artificial Insemination Center (LAIC). Repeatability for volume, motility, fresh semen concentration and frozen semen production was evaluated by intraclass correlation method. The repeatability values of LAIC for volume, motility, fresh semen concentration and frozen semen production were 0.60; 0.54; 0.37 and 0.47. The repeatability values of SAIC for volume, motility, fresh semen concentration and frozen semen production were 0.54; 0.30; 0.43 and 0.29. The linear correlation value between volume, motility and fresh semen concentration with the amount of semen produced per collections were 0.41, 0.36, and 0.58. Concentration was the most factors influencing the number of frozen semen produced. The effectiveness of the selection of Holstein's frozen semen producing could be determined by the value of repeatability and the phenotypic correlation among semen quality traits such as volume, motility, concentration and frozen semen production.

scholarly journals Sperm protein markers for Holstein bull fertility at National Artificial Insemination Centers in Indonesia

2020 ◽  
Vol 13 (5) ◽  
pp. 947-955
Author(s):  
Zulfi Nur Amrina Rosyada ◽  
Mokhamad Fakhrul Ulum ◽  
Ligaya I. T. A. Tumbelaka ◽  
Bambang Purwantara
Keyword(s):  
Artificial Insemination ◽  
Sperm Quality ◽  
High Fertility ◽  
Fertility Level ◽  
Sperm Protein ◽  
Sperm Proteins ◽  
Frozen Semen ◽  
Sds Page ◽  
Microscopic Evaluation ◽  
Holstein Bulls

Background and Aim: Holstein cows and heifers are widely bred in Indonesia by artificial insemination (AI) to increase population and milk production. Sperm fertility is modulated by genetic factors, but the analysis of sperm quality is still based on macro- and microscopic characteristics. This study aimed to analyze both sperm quality and proteins of Holstein bulls at different fertility levels. Materials and Methods: The frozen semen samples were collected from the Indonesia National AI Center. They were classified based on the reproductive efficiency data and were grouped into high fertile (HF) and low fertile (LF). Sperm qualities were evaluated by microscopic evaluation. The Holstein sperm proteins were extracted using phenylmethanesulfonyl fluoride as a protease inhibitor and the benzidine detergent extraction method. Discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was conducted to analyze the molecular weights (MWs) of the sperm proteins. The data obtained were analyzed by a t-test using the one-factor bull fertility level, and Spearman's correlation analysis was used to identify the correlation between the sperm microscopic evaluation parameters and protein bands. Results: The sperm motility post-freeze thawing was not significantly different between the HF and LF (p>0.05). The HF level had a higher percentage of viability, intact plasma membrane integrity, and intact acrosomes than the LF (p<0.05). Five protein bands were found in the SDS-PAGE of sperm proteins of Holstein bulls with different concentrations. Sperm proteins with MWs of 17.51 kDa, 14.87 kDa, 33.71 kDa, and 41.97 kDa were abundant in the Holstein bulls with an HF level, while 55 kDa proteins were abundant in the LF level of Holstein bulls. The sperm of Holstein bulls in the HF level contained proteins of about 33.71 kDa that were not detected in the LF. Conclusion: The sperm protein with a molecular weight of 33.71 kDa was predicted to be a specific protein biomarker that influences bull fertility. Sperm fertilization abilities were also determined by the sperm proteins, the morphology of sperm acrosomes, and the quality of plasma membranes. This method can be used to select bulls with high fertility to increase the population of Holstein bulls.

Comparison of uterine inflammatory response of jennies after artificial insemination with vitrified or frozen-thawed donkey sperm

2020 ◽  
Vol 89 ◽  
pp. 103036
Author(s):  
M. Diaz-Jimenez ◽  
A. Rota ◽  
J. Dorado ◽  
D. Panzani ◽  
D. Fanelli ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Artificial Insemination

scholarly journals Successful embryo transfer from Hokkaido native pony after artificial insemination with frozen semen

2019 ◽  
Vol 81 (2) ◽  
pp. 241-244 ◽  
Author(s):  
M A HANNAN ◽  
Shingo HANEDA ◽  
Yasuyuki ITAMI ◽  
Sohei WACHI ◽  
Tomoko SAITOH ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Artificial Insemination ◽  
Frozen Semen

scholarly journals Estrus and pregnancy rate of Simmental-Ongole Crossbred and Ongole Grade heifer after being synchronized and inseminated

2018 ◽  
Vol 43 (4) ◽  
pp. 438
Author(s):  
S. Sutiyono ◽  
D. Samsudewa ◽  
A. Suryawijaya
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Reproductive Organs ◽  
Estrus Synchronization ◽  
Purposive Sampling ◽  
Frozen Semen ◽  
Research Population ◽  
Rectal Palpation

The aim of the study was to examine the synchronization of estrus and artificial insemination in heifers of Ongole grade and Simental-Ongole grade. The research used 19 Ongole Grade (OG) and 18 Simental-Ongole Crossbred (SOC) heifers. This research applied purposive sampling to determine the research population. The criteria to select the population were heifers which have in mature body. The normality of heifer’s reproductive organs were identified by performing rectal palpation, then 50 mg of medroxy progesterone acetate on vaginal sponge was used to synchronized estrus. The estrus heifer was inseminated using frozen semen. The observed parameters were the number of heifers with normal and abnormal reproductive organs, estrus sign and pregnancy. Data were analyzed descriptively. This study showed that OG and SOC with abnormal reproductive organs was 1 heifer (5.56%) and 7 heifers (36.84%), respectively. The responses of estrus synchronization in heifers with normal and abnormal reproductive organs were 94.17% and 100% in OG, and 100% and 42.86% in SOC. Meanwhile, the pregnancy rate was 70.59 and 0% in OG, and 50.00 and 0% in SOC. In conclusion, the number of SOC heifers with abnormal reproductive organs is higher than OG. Estrus synchronization using 50 mg medroxy progesterone acetate increase the amount of estrus and pregnancy in heifers with normal reproductive organs.

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