scholarly journals Comparative study of semen quality traits between Etawah grade and Senduro bucks

2021 ◽  
Vol 888 (1) ◽  
pp. 012022
Author(s):  
N Isnaini ◽  
A A Hakim ◽  
D Amertaningtyas ◽  
H E Sulistyo ◽  
A Irsyammawati ◽  
...  
Keyword(s):  
Comparative Study ◽  
Artificial Insemination ◽  
Recovery Rate ◽  
Semen Quality ◽  
T Test ◽  
Quality Traits ◽  
Spermatozoa Motility ◽  
Frozen Semen ◽  
Semen Volume

Abstract This study compared the semen quality traits between Etawah grade (EGB) and Senduro bucks (SB) raised in Singosari National Artificial Insemination Center, Indonesia. A total of 96 ejaculated semen were collected from three EGB and three SB with an average age of three years. The ejaculates were evaluated for semen volume (SV), spermatozoa concentration (SC), total spermatozoa (TS), spermatozoa motility (SM), post-thawing spermatozoa motility (PTSM), the recovery rate of spermatozoa motility (RRSM), and frozen semen production (FSP). Data of SV, SM, PTSM, and RRSM were compared using the Mann-Whitney U test. While the data of SC, TS, and FSP were analyzed using an independent t-test. The results showed that SV, SM, PTSM, and RRSM were not significantly differed (P>0.05) between breeds. Whereas, SC, TS, and FSP of EGB were significantly higher (P<0.05) than SB. Therefore, it can be concluded that Etawah grade bucks can produce higher total spermatozoa and as a result higher frozen semen production for artificial insemination as compared to Senduro bucks.

scholarly journals PENGARUH FALSE MOUNTING TERHADAP KUALITAS SEMEN SAPI LIMOUSIN DAN SIMMENTAL DI BALAI INSEMINASI BUATAN LEMBANG

ZOOTEC ◽  
2019 ◽  
Vol 39 (2) ◽  
pp. 417
Author(s):  
Yuli Siska Kafiar ◽  
Sri Adiani ◽  
Agustinus Lomboan ◽  
H.F.N. Lapian
Keyword(s):  
Artificial Insemination ◽  
Semen Quality ◽  
Mass Movement ◽  
T Test ◽  
Average Volume ◽  
Concentration Data ◽  
Spermatozoa Motility ◽  
Bull Semen ◽  
Semen Volume

THE EFFECT OF FALSE MOUNTING ON SEMEN QUALITY OF LIMOUSIN AND SIMMENTAL BULL IN LEMBANG ARTIFICAL INSEMINATION CENTER. The purpose of this study was to determine the effect of false mounting on the quality of Limosin and Simental bull semen at the Lembang Artificial Insemination Center. The 55 individu of Limousin and 55 Simmental bull aging 5-9 years old have been used in thos study. The data have been collected directly in the Artificial Insemination Center Lembang - West Java Province. The data obtained in the form of macroscopic and microscopic observations on Limousin and Simmental bull include volume, pH, mass movement, spermatozoa motility and spermatozoa concentration. Data were analyzed descriptively by calculating the standard deviations, correlations coefficients and the T test. The variables measured in this study were macroscopic and microscopic quality of semen. The results of this study indicate that the effect of false mounting on the average semen volume of Limousin and Simmental bull is maximum at 3, 4, and 5 times false mounting but there was not significantly difference between the amount of false mounting, as well as the average volume, pH, mass movement, motility of spemetozoa and concentration spermatozoa are not significantly different. It was concluded that false mounting in Limousin and Simmental bulls in Lembang Artificial Insemination Center had no significantly effect on the quality of macroscopic and microscopic semen. Keywords: False Mounting, Cement Quality, Limousin Cattle, Simmental Cows.

scholarly journals Repeatability and Phenotypic Correlation Among Semen Quality Traits in Holstein Bulls

2018 ◽  
Vol 42 (4) ◽  
Author(s):  
Argi Argiris ◽  
Siswanto Imam Santoso ◽  
Yon Supri Ondho ◽  
Edy Kurnianto
Keyword(s):  
Artificial Insemination ◽  
Semen Quality ◽  
Intraclass Correlation ◽  
Correlation Method ◽  
Phenotypic Correlation ◽  
Quality Traits ◽  
Frozen Semen ◽  
Holstein Bulls ◽  
Fresh Semen ◽  
Selection Of

The purpose of this research was to analysis the value of repeatability and correlation among the traits affecting the production of frozen semen from Holstein’s bull in Indonesia. Repeatability and correlation were calculated based on the data of frozen semen production of 15.699 records from 44 Holstein bulls at Singosari Artificial Insemination Center (SAIC) and 8.935 records from 39 Holstein bulls at Lembang Artificial Insemination Center (LAIC). Repeatability for volume, motility, fresh semen concentration and frozen semen production was evaluated by intraclass correlation method. The repeatability values of LAIC for volume, motility, fresh semen concentration and frozen semen production were 0.60; 0.54; 0.37 and 0.47. The repeatability values of SAIC for volume, motility, fresh semen concentration and frozen semen production were 0.54; 0.30; 0.43 and 0.29. The linear correlation value between volume, motility and fresh semen concentration with the amount of semen produced per collections were 0.41, 0.36, and 0.58. Concentration was the most factors influencing the number of frozen semen produced. The effectiveness of the selection of Holstein's frozen semen producing could be determined by the value of repeatability and the phenotypic correlation among semen quality traits such as volume, motility, concentration and frozen semen production.

scholarly journals Evaluation of semen quality of buffalo frozen semen produced by Artificial Insemination Center

2018 ◽  
Vol 43 (1) ◽  
pp. 26 ◽  
Author(s):  
H. C. Mahendra ◽  
D. Samsudewa ◽  
Y. S. Ondho
Keyword(s):  
Artificial Insemination ◽  
Recovery Rate ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Animal Husbandry ◽  
Mean Value ◽  
National Standard ◽  
Frozen Semen ◽  
Standard Values

This study aimed to evaluate and compare the quality of post thawing spermatozoa of buffalo frozen semen produced by artificial insemination centers on standard values, and proposed reference values (PRV). Materials of the research were 60 samples of straws obtained from three Artificial Insemination Center, which are each 20 straws, respectively. Parameters observed were motility, concentration, longevity, plasma membrane integrity (PMI), acrosome integrity (AIn) and recovery rate. The obtained data were tested by Z test then presented as mean ± standard deviation. The research results from three artificial insemination center showed that motility mean was 45.00±3.07%, concentration mean was 26.09±3.11 x106 cells/0.25mL, longevity mean was 10.38±0.75%/hour, PMI mean was 45.86±10.67%, AI mean was 73.99±5.28% and recovery rate mean was 64.38±5.16%. The conclusions of this research were the motility and concentration mean were higher than Indonesian National Standard (INS), longevity mean was lower than Department of Animal Husbandry, Dairying and Fisheries (DADF) standard but PMI and AIn mean were higher, the mean value of recovery rate was higher than of proposal value. 

scholarly journals Effect of Various Thawing Times and Temperatures on Frozen Semen Quality in Plastic Straws of Artificial Insemination of Bulls in Iraq

2018 ◽  
Vol 30 (2) ◽  
pp. 99-102
Author(s):  
Asaad H. I. Al-Edany
Keyword(s):  
Artificial Insemination ◽  
Light Microscope ◽  
Semen Quality ◽  
Practical Method ◽  
Sperm Cells ◽  
Frozen Semen

The present study was designed to determine the best practical method of semen thawing which could be applied to frozen semen of bulls in straw produced by Artificial Insemination Center, Iraq. Frozen semen was thawed at 15°C for 30 sec, 40°C for 10 sec and 50°C for 5 sec. individual  motility, live, and dead sperm cells was assessed using light microscope. Results revealed that number, motility, and  live of sperm cells, were significantly higher at the 50°C for 5 sec than other thawing temperatures In conclusion, it is recommended to use a temperatures of   50°C for 5 sec and to dissolve  the frozen semen because it gave the best quality semen after thawing  and easy to utilize in field.

scholarly journals Kaji Banding Kualitas Semen Segar Empat Genetik Ayam Lokal Indonesia (COMPARATIVE STUDY ON THE QUALITY OF FRESH SEMEN OF FOUR GENETIC LOCAL CHICKEN IN INDONESIA)

2019 ◽  
Vol 20 (3) ◽  
pp. 397
Author(s):  
Junaedi Junaedi ◽  
Husnaeni Husnaeni
Keyword(s):  
Comparative Study ◽  
Spermatozoa Motility ◽  
Genetic Groups ◽  
Randomized Design ◽  
Semen Volume ◽  
Completely Randomized Design ◽  
Local Chicken ◽  
Fresh Semen ◽  
Better Than

This study was conducted to examine the comparison the quality of spermatozoa in four genetic groups of poultry (Pelung chicken, Nunukan chicken, Sentul chicken, and Bangkok chicken). The parameters of this study were the concentration of spermatozoa, motility, viability, semen volume, color, and consistency. The study was designed using a Completely Randomized Design (CRD) with four local chicken nations and four replications. The results showed that the volume of Nunukan chicken semen was 0.32±0.01 mL/ejaculation, Bangkok chicken was 0.31±0.01 mL/ejaculate, Sentul chicken was 0.15±0.02 mL/ejaculate and Pelung chicken was 0.23±0.02 mL/ejaculation. the color of fresh semen is white (Pelung chicken, Nunukan chicken and Sentul chicken) except chicken Bangkok has the color of cream semen. Pelung chicken has a concentration of spermatozoa 5,043.33±51 million/mL, Nunukan chicken 3,250.22 ±45 million/mL, Sentul chicken 3,002.87±67 million/mL, and Bangkok chicken 3,002.87 ± 67 million/mL. Motility of Pelung chicken 84.,69 ± 1.12%, Bangkok chicken 82.35±1.85%, Nunukan chicken 77.74±1.57% and Sentul chicken 77.64±1.65%. Viability spermatozoa of Sentul chicken was 90.35 ± 1.21%, Bangkok chicken was 90.64 ± 1.16%, Pelung chicken was 89.17 ± 1.23% and Nunukan chicken was 86.29 ± 1.15%. It can be concluded that the motility spermatozoa of Pelung chicken and Bangkok chicken is better than the spermatozoa motility of Nunukan chicken and Sentul chicken. The viability of Sentul chicken and Bangkok chicken was higher compared to the viability semen of chicken Pelung and chicken Nunukan.

Artificial insemination of small ruminants — A review

2012 ◽  
Vol 60 (1) ◽  
pp. 115-129 ◽  
Author(s):  
Vera Faigl ◽  
Nóra Vass ◽  
András Jávor ◽  
Margit Kulcsár ◽  
László Solti ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Review Paper ◽  
Semen Quality ◽  
Small Ruminants ◽  
Livestock Production ◽  
Success Rates ◽  
Assisted Reproductive Technique ◽  
Frozen Semen ◽  
Semen Processing ◽  
Reproductive Technique

Artificial insemination (AI) can undoubtedly be regarded as the oldest and most widely used assisted reproductive technique/technology (ART) applied in livestock production and it is one of the most important ARTs. The three cornerstones of its application are that it is simple, economical and successful. Artificial insemination offers many well-known benefits for producers. Fresh, fresh + diluted + chilled and frozen semen can be used for AI in small ruminants. To ensure its successful use, the AI technique must be selected on the basis of the type of semen planned to be used. This review paper gives a detailed overview of semen processing and its effects on semen quality, as well as of the AI techniques applied in small ruminants and their success rates.

scholarly journals The Influence of Breed and Type of Extender on the Quality of Bull Semen

2020 ◽  
Vol 21 (2) ◽  
pp. 64
Author(s):  
Iman Sukirman ◽  
Eros Sukmawati ◽  
Siti Darojah Rasad ◽  
Nurcholidah Solihati
Keyword(s):  
Plasma Membrane ◽  
Semen Quality ◽  
Block Design ◽  
Egg Yolk ◽  
Spermatozoa Motility ◽  
Bull Semen ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Two Factors

This study aimed to determine the influence of breed and type of extenders on frozen semen quality of cows at BIB Lembang. The experimental study was conducted in a Factorial Randomized Block Design (RBD) with two factors. The first factor was four cow breeds, i.e. Ongole Cross (PO), Brahman (BR), Simmental (SM) and Limousin (LM), and the second factor was two types of extender, i.e. Skim-Egg Yolk (SKT) and AndroMed® (AND), all repeated four times. The observed variables were percentage of spermatozoa motility and intact plasma membrane (IPM). All data obtained were analyzed using a general linear model (IBM SPSS ver. 23). The results demonstrated an interaction between breed and the type of diluent to motility. Breeds showed significantly different motility but non-significantly different intact plasma membrane (MPU) of semen. The type of diluent did not significantly affect motility and intact plasma membrane (MPU) of the frozen semen. The effect of the breed on BR motility was lower and significantly different from PO, LM and SM. The types of diluent did not significantly affect motility, MPU. The results showed that SKT was lower than AND, it was indicative effect of breed on intact plasma membrane (MPU) PO was lower than BR, LM and SM and the effect of the type of diluent on whole plasma membrane (MPU) AND is lower than SKT. It can be concluded that breed influences the motility of semen. The lowest motility reduction in frozen semen is Brahman cattle by using skim-egg yolk extender.

scholarly journals Fertility and Sperm Quality of 1- and 2-year-Old Native Ganders Used for Natural Mating and Artificial Insemination

2021 ◽  
Author(s):  
Mehmet Akif BOZ ◽  
Hatice BAŞ ◽  
Musa SARICA ◽  
Kadir ERENSOY
Keyword(s):  
Sperm Motility ◽  
Artificial Insemination ◽  
Egg Production ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Day Length ◽  
Quality Traits ◽  
Female Ratio ◽  
Semen Volume ◽  
Natural Mating

Abstract In the present study, the fertility rate and egg-hatching results from either natural mating or artificial insemination using 1- and 2-year-old domestic Turkish geese were compared. Sperm quality traits of 1- and 2-year-old ganders used for mating were determined. The study comprised 72 two-year-old females, 12 one-year-old, and 12 two-year-old ganders at the beginning of the laying period. Thirty-six female geese were mated naturally (18 with 1-year-old ganders, 18 with 2-year-old ganders); the remaining 36 were artificially inseminated (18 with 1-year-old ganders, 18 with 2-year-old ganders). Twenty-four ganders were separated into groups (12 for natural mating; 12 for artificial insemination). The male-to-female ratio in both mating protocols was 1:3. The geese were housed during the laying period in a natural and artificially ventilated house under natural lighting (increased day length). Quality traits, such as semen volume, sperm concentration, sperm quality factor (SQF), sperm motility, and some sperm morphological properties were determined. Fertility was higher in the groups containing 2-year-old ganders than in those using 1-year-old ganders (P < 0.05). The effect of insemination on semen volume, sperm concentration, SQF, sperm motility, total live sperm, normal sperm, macrocephalus sperm, and dead sperm was determined to be significant (P < 0.05). As a result, there is a need to improve the egg production and broody behavior traits of domestic Turkish geese. In addition, the use of 2-year-old geese in artificial insemination is appropriate and will contribute positively to the breeding and selection process.

scholarly journals Individual Variation in Semen Characteristics of Murrah Buffalo Bulls

2020 ◽  
Vol 9 (3S) ◽  
pp. 58-60
Keyword(s):  
Sperm Motility ◽  
Individual Variation ◽  
Artificial Insemination ◽  
Recovery Rate ◽  
Sperm Concentration ◽  
Murrah Buffalo ◽  
Frozen Semen ◽  
Semen Characteristics ◽  
The Individual ◽  
Fresh Semen

This study evaluated the variation in semen characteristics between two individual Murrah buffalo bulls. Two hundred and ninety semen samples were involved in this study. The results showed that most of the semen had a milky white color with moderate consistency. The Bull B1 tended to have higher proportion of creamy color and moderate consistency than the Bull B2. The ejaculatory volume is varied (P<0.01) between Bull B1 and Bull B2. However, semen pH and sperm concentration of Bull B1 and B2 were equal (P>0.05). The individual bull had a highly significant effect (P<0.01) on sperm motility of fresh semen. The individual variation (P<0.05) also found on sperm motility of before freezing. Whereas, sperm motility of post-thawing semen and recovery rate value were not significantly influenced (P>0.05). Moreover, it was found that the frozen semen production differed (P<0.01) between two individual bulls, with bull B1 had higher production than the bull B2. This study clearly demonstrates that the frozen semen production of Murrah buffalo is highly dependent on the individual bull. However, the variation in frozen semen production between two bulls studied here is still qualified to be used for artificial insemination.

scholarly journals The Effect of Centrifugation on Semen Quality of Peranakan Ettawah Goat's Post Thawing

2010 ◽  
Vol 4 (2) ◽  
Author(s):  
Indiah Indiah ◽  
Sri Wahjuningsih
Keyword(s):  
Semen Quality ◽  
Lower Layer ◽  
Vitro Fertilization ◽  
Spermatozoa Motility ◽  
Tukey Test ◽  
Abnormal Morphology ◽  
Frozen Semen ◽  
Experiment Method

The aim of the research was to determine the optimum of centrifugation on the quality of Peranakan Ettawah goat's spermatozoa prepared for in vitro fertilization preparation.The research of material was frozen semen ofPeranakan Ettawah goat produced by Artificial Insemination Center in Singosari with minimum post thawing motility in 40%. The method of the research was an experiment method with the four treatment, i.e: 1000 (P1), 1500 (P2), 2000 rpm (P3) of different centrifugation. The variable observes were spermatozoa motility, spermatozoa viability, and abnormal morphology of spermatozoa. Data obtain was analyzed statistically using Completely Randomize Design and continued with Tukey test. The result showed that on the upper layer of each treatment (P1, P2, and P3) obtained 55,72±3,55; 69,55±3,35; and 55,19±2,72% for spermatozoa motility, 62,99±5,87; 73,99±4,36; and 57,39±9,22% for spermatozoa viability, and 7,19±3,64; 8,84±2,65; and 6,40±3,00% for abnormal morphology of spermatozoa, descriptively. While on the lower layer the result showed 55,59±3,99; 68,63±3,88; and57,61±2,20% for spermatozoa motility, 70,05±7,47; 77,44±1,08; and 69,93±11,98% for spermatozoa viability, and 10,36±6,20; 9,55±3,27; and 8,09±2,80% for abnormal morphology of spermatozoa, desciptively. It was concluded that centrifugation in 1500 rpm showed the highest motility and viability on the upper layer and lower layer.

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