scholarly journals The role of transcription factor FoxP3 as a predictor of acute and chronic urticaria in children

2021 ◽  
Vol 12 (3) ◽  
pp. 50-54
Author(s):  
S. V. Maltsev ◽  
L. P. Sizyakina ◽  
A. A. Lebedenko
Keyword(s):  
Transcription Factor ◽  
Chronic Urticaria ◽  
Chronic Spontaneous Urticaria ◽  
Immunological Methods ◽  
Control Group ◽  
Acute Urticaria ◽  
Clinical Methods ◽  
Regulatory Lymphocytes

Objective: To study the transcription factor FoxP3 in children with acute and chronic spontaneous urticaria as a possible predictor of the severity and chronicity of urticaria.Materials and Methods: A total of 264 children of both sexes aged from 6 to 16 years old with diff erent variants of urticaria course were examined. Clinical methods of the study included the analysis of anamnestic data and an objective examination of the child with the determination of the severity of urticaria. Immunological methods of the study included the identifi cation of T-regulatory lymphocytes with the CD4+CD25+ Foxp3+CD45+immunophenotype.Results: A signifi cant decrease in the level of transcription factor FoxP3 was found in children with severe acute urticaria and chronic urticaria compared to the control group.Conclusion: Th e degree of reduction in the level of FoxP3 signifi cantly aff ected the likelihood of the development of a severe course of acute urticaria and possible chronization of the disease.

Correction of Psychological Status by Family Focused Technologies

2016 ◽  
pp. 129-131
Author(s):  
Serhii Vdovychenko
Keyword(s):  
Traditional Approach ◽  
Married Couple ◽  
Psychological Status ◽  
Control Group ◽  
Premature Births ◽  
The Family ◽  
Risk Patients ◽  
Individual Preparation

The objective: to show a role of the family focused technologies in correction of psychological status and depression of frequency of pathology of pregnancy at women of high obstetric risk. Patients and methods: for determination of efficiency of prophylaxis of pathology of pregnancy on the basis of use of the family focused technologies complex clinical-psychological and laboratory and tool examination of 300 women with factors of obstetric risk which were divided into two groups was conducted. In the main group – 182 women with motivation on partner labors to which provided training on system of individual preparation of married couple to labors. The control group consisted of 118 women with a traditional approach to pain management that have not been prenatal training. Results. Use of the family focused technologies during pregnancy allows correction of psyhological status and to reduce significantly the frequency of the main complications of pregnancy, especially not incubation and premature births. Conclusion. In our opinion, the technique is simple, available and can widely be used in practical health care at women with high obstetric risk.

scholarly journals Polymorphisms in Genes Involved in Steroidogenesis in the Development of Severe Acne

2021 ◽  
Vol 11 (3) ◽  
pp. 275-280
Author(s):  
Alexander G. Rumyantsev ◽  
Olga M. Dеmina
Keyword(s):  
Molecular Genetic ◽  
Hair Follicles ◽  
Genetic Diagnostics ◽  
Control Group ◽  
Severe Acne ◽  
High Throughput Dna Sequencing ◽  
Molecular Genetic Diagnostics ◽  
Gene 4

Background: Acne is a multifactorial disease, in the pathogenesis of which one of the leading factors is the excessive effect of androgens on the hair follicles (HFs) and sebaceous glands (SGs), along with hypersecretion of sebum, pathological follicular hyperkeratosis and an inflammatory response. The search for genotypic markers in patients with varying severity of acne is a difficult task due to the multifactorial pathogenesis and the role of trigger factors in the formation of acne. The aim of this study was to determine SNPs within 3 genes involved in steroidogenesis (MVK, ARPC1B, and CA2) in patients with severe acne. Methods and Results: The study included 70 patients (42 men and 28 women) aged between 15 and 46 years (the median age - 22.1 years). The main group (MG) included 50 patients (29 men and 21 women) with severe acne. The control group (CG) consisted of 20 apparently healthy individuals (13 men and 7 women). Molecular-genetic diagnostics was carried out by the method of high-throughput DNA sequencing (next-generation sequencing). Our study showed that severe acne is associated with 12 polymorphic loci of the MVK gene (4 SNPs in exons and 8 SNPs in introns), 7 SNPs of the ARPC1B gene (2 SNPs in exons and 5 SNPs in introns), and 9 SNPs of the CA2 gene (3 SNPs in exons and 6 SNPs in introns). Conclusion: The revealed features of the SNPs within the MVK, ARPC1B, and CA2 genes in patients with severe acne probably indicate a hereditary determination of steroidogenesis in acne.

scholarly journals Amelioratory Effect of Nanoconjugated Vancomycin on Spleen during VRSA-Induced Oxidative Stress

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Subhankari Prasad Chakraborty ◽  
Santanu KarMahapatra ◽  
Sumanta Kumar Sahu ◽  
Panchanan Pramanik ◽  
Somenath Roy
Keyword(s):  
Oxidative Stress ◽  
Reduced Glutathione ◽  
Treated Group ◽  
Control Group ◽  
Myeloperoxidase Activity ◽  
Viable Bacteria ◽  
Antioxidant Effects ◽  
Potential Use

Objective. The aim of the present study was to evaluate the possible antioxidant effects of nanoconjugated vancomycin against VRSA infection on select makers of oxidative damage and antioxidant status in spleen. Methods. A coagulase-positive VRSA strain was used for this study. VRSA infection was developed in Swiss mice by intraperitoneal injection of 5 × 106 CFU/mL bacterial solutions. VRSA-infected mice were treated with nanoconjugated vancomycin at its effective dose for 10 days. After decapitation, blood was used for determination of viable bacteria count and spleen was excised from control and experimental groups, homogenized and used for different biochemical estimations. Results. Nitrate level, myeloperoxidase activity, lipid peroxidation, protein oxidation, oxidized glutathione, and DNA fragmentation level were increased significantly (P<0.05) in spleen of VRSA-infected group as compared to control group, and reduced glutathione level, activity of SOD, CAT, GPx, GR, and GST were decreased significantly (P<0.05); which were increased or decreased significantly (P<0.05) near to normal in nanoconjugated vancomycin-treated group. Conclusion. These findings suggest the potential use and beneficial role of nanoconjugated vancomycin against VRSA-infection-induced oxidative stress and DNA damage in spleen.

Role of Plasma miRNA-501 Expression Profile as a Marker of Hepatocellular Carcinoma (HCC) Progression With Hepatitis C Virus Infection

2019 ◽  
Vol 152 (Supplement_1) ◽  
pp. S134-S134
Author(s):  
Mohamed Shedid ◽  
Mohamed Abdelmonem ◽  
Ayman Boraik ◽  
Alaa Elmetwalli ◽  
Ahmed Esmael
Keyword(s):  
Rna Extraction ◽  
Control Group ◽  
Hepatitis C Virus Infection ◽  
Rt Pcr ◽  
The World ◽  
Noninvasive Biomarker ◽  
Biological Aspects ◽  
Plasma Mirna

Abstract Introduction HCV is a health problem that confronts many countries in the world. Those patients will develop complications like cirrhosis and HCC, which is one of the most common cancers in the world, especially in Egypt, and considered the third leading cause of death worldwide. The prognosis of HCC is still dismal due to the late diagnosis. miRNAs are small, short noncoding RNAs, which have roles in the diagnosis of HCC. In our study, we focus on biological aspects of miRNAs. We report that miR-501 is strongly expressed and observed in the process of HCC development. miR-501 regulation is important as an oncofetal relevant to the diagnosis of HCC. Method This study was conducted on 100 adult patients; 25 patients were positive for anti-HCV and 25 patients were negative for HCV and enrolled as a control group. Patients were categorized into three groups: fibrosis (n = 25), CHC (n = 25), and HCC (n = 25) related to HCV evident by CT abdomen. All patients and controls were subjected to full clinical assessment and laboratory investigation. Blood (8 mL) was withdrawn from subjects, and 3 mL was collected in EDTA tubes for processing total RNA extraction and miRNA. The remaining 5 mL was left for determination of biochemical parameters. miRN-501 expression levels were determined by RT-PCR. Results The data revealed a significant increase in levels of AST, ALT, ALP, and CBC in both HCC and CHC groups compared to controls. The results of miRNA expression showed that miR-501 was higher in the HCC group than non-HCC group at P1.1. Conclusion miR-501 can be used as a noninvasive biomarker for early diagnosis of HCC among patients with HCV on account of its affectability for HCC.

scholarly journals Down-regulation of miR-10a-5p promotes proliferation and restricts apoptosis via targeting T-box transcription factor 5 in inflamed synoviocytes

2018 ◽  
Vol 38 (2) ◽  
Author(s):  
Nazim Hussain ◽  
Wenhua Zhu ◽  
Congshan Jiang ◽  
Jing Xu ◽  
Manman Geng ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Transcription Factor ◽  
Quantitative Polymerase Chain Reaction ◽  
Cell Counting ◽  
Control Group ◽  
Down Regulation ◽  
T Box ◽  
Proliferation And Apoptosis ◽  
Polymerase Chain

Synoviocytes from rheumatoid arthritis (RA) patients share certain features with tumor cells, such as over proliferation and invasion. Anomalous microRNA (miRNA) expression may participate in the pathogenesis of RA in different ways. The objective of the present study was to observe the role of miR-10a-5p targeting T-box transcription factor 5 (TBX5) gene on synoviocyte proliferation and apoptosis in RA. Human synovial sarcoma cell line, SW982 cells stimulating with interleukin-1β (IL-1β) were transfected with miR-10a-5p mimic and siRNA of TBX5. The real-time quantitative polymerase chain reaction (RT-qPCR) and Western blotting analysis were used to evaluate the expression level of miR-10a-5p and TBX5 in SW982 cells respectively. Further, the proliferation and apoptosis of SW982 cells after treatment were determined by cell counting kit (CCK-8) and flow cytometry analysis respectively. We found that the miR-10a-5p showed down-regulated while TBX5 showed up-regulated expression in synoviocytes after stimulation with IL-1β. The miR-10a-5p mimic treatment showed a decline in cell proliferation while the increased rate of cell apoptosis as compared with control. Moreover, knockdown of TBX5 favored the apoptosis and reduced the cell proliferation as compared with control group. We conclude that down-regulation of miR-10a-5p promotes proliferation and restricts apoptosis via targeting TBX5 in inflamed synoviocytes.

Role of thyroid transcription factor-1 in the diagnosis of feline lung–digit syndrome

2016 ◽  
Vol 19 (4) ◽  
pp. 477-483 ◽  
Author(s):  
Riccardo Finotello ◽  
Carlo Masserdotti ◽  
Gianna Baroni ◽  
Lorenzo Ressel
Keyword(s):  
Transcription Factor ◽  
Control Group ◽  
Study Group ◽  
Ciliated Epithelium ◽  
Neoplastic Tissue ◽  
Thyroid Transcription Factor 1 ◽  
Thyroid Transcription Factor ◽  
Formalin Fixed Paraffin Embedded ◽  
Transcription Factor 1

Objectives The aim of this study was to investigate the role of thyroid transcription factor-1 (TTF-1) in the diagnosis of feline lung–digit syndrome (FLDS) and to investigate the associations between the morphological features of FLDS and TTF-1 expression. We also compared the reliability of TTF-1 and transmission electron microscopy (TEM) in establishing the diagnosis of FLDS. Methods Histology records of feline digit tumours were retrieved, including patients from 2008–2015. If formalin-fixed, paraffin-embedded tissues were available for review, patients were included in the study. As a control group we included 12 feline primary tumours of the digits. All the histological slides of the study group were blindly reviewed by the same veterinary pathologist. Representative sections of the lesions were selected for immunohistochemistry (IHC) analysis. To confirm the respiratory origin of the neoplastic tissue, TEM was used as a gold standard in all cases. Results Five cases of FLDS were included. TTF-1 was weakly to moderately positive in 60% of the cases, showing no correlation with the microscopic presence of ciliated epithelium. When IHC results were combined with the presence of cilia, 80% of the cases from the study group could be identified as FLDS. TEM confirmed the presence of ciliated epithelium in all five cases, confirming the respiratory origin of the neoplastic tissue and therefore the diagnosis of FLDS. Conclusions and relevance TTF-1 expression is maintained in FLDS. While the combination of TTF-1 and identification of cilia confirms FLDS, TEM should be considered in those cases where diagnosis is uncertain and FLDS is suspected.

scholarly journals REMISSION OF A CASE OF MULTIPLE HYMENOPTERA STINGS-ASSOCIATED CHRONIC URTICARIA DURING VENOM IMMUNOTHERPY

2021 ◽  
Author(s):  
Marco Dubini ◽  
Valerio Pravettoni ◽  
Federica Rivolta ◽  
Giulia Segatto ◽  
Riccardo Asero ◽  
...  
Keyword(s):  
Chronic Urticaria ◽  
Venom Immunotherapy ◽  
Acute Urticaria ◽  
Hymenoptera Sting ◽  
Wasp Stings

The role of Immunotherapy in Chronic Urticaria is unclear, except for isolated circumstances. Hymenoptera sting causes acute urticaria and no report of CU after Hymenoptera sting can be found in the literature. We describe a case of onset of CU after multiple wasp stings that remitted during venom immunotherapy.

scholarly journals Determination of Cholesterol and Triglyceride Concentrations in Serum Extracellular Vesicles Using Commercial Kits

2021 ◽  
Vol 4 (2) ◽  
pp. e00148
Author(s):  
A.A. Yakovlev ◽  
V.D. Antonov ◽  
T.A. Druzhkova ◽  
A.B. Guekht ◽  
N.V. Gulyaeva
Keyword(s):  
Blood Serum ◽  
Healthy Volunteers ◽  
Extracellular Vesicles ◽  
The Body ◽  
Control Group ◽  
Sensitivity Threshold ◽  
Commercial Kits ◽  
Average Size

Exosomes and microvesicles, collectively referred to as small extracellular vesicles (sEV) are vesicles with an average size of about 100-150 nm. Currently, the role of sEV in various aspects of signaling in the body is being actively investigated; in addition, sEV can often serve as markers of various pathologies. The active study of the sEV composition is continuing. In this study we have demonstrated that in sEV it is possible to determine cholesterol and triglycerides concentration by using commercial kits designed for serum. The technique was tested on sEV from the blood of patients diagnosed with depression and on healthy volunteers. No differences were found in the concentration of cholesterol and triglycerides in mEV from the blood serum of depressed patients and the control group. The concentration of cholesterol and triglycerides in the samples is several times higher than the sensitivity threshold of the methods set by the manufacturer of the kits.

scholarly journals Features of innate immunity in children with moderate course of acute urticaria

2018 ◽  
Vol 15 (3) ◽  
pp. 53-58
Author(s):  
S V Maltsev ◽  
L P Sizyakina ◽  
A A Lebedenko
Keyword(s):  
Innate Immunity ◽  
Activity Index ◽  
The Body ◽  
Immunological Methods ◽  
Total Serum ◽  
Parasitic Infections ◽  
Control Group ◽  
Healthy Children ◽  
Serum Ige ◽  
Acute Urticaria

Background. The prevalence of acute urticaria in children remains high. Urticaria is polietiologic disease, maintaining the homeostasis of the body is carried out by close cooperation of various parts of the immune system. The aim of this investigation was to study the features of innate immunity in children with moderate acute urticaria. Methods. To achieve this goal 71 children with moderate acute urticaria were examined. The control group included 30 healthy children of both sexes of the same age. Clinical methods of the study included disease history analysis, phisical examination of the child with determination of the degree of urticaria severity (calculation of the urticaria activity index within 7 days). Immunological methods included NK-lymphocytes, CD14+CD282+, CD14+CD284+, CD14+CD289+, serum IgE level, lactoferrin, IFN-y, IL-4, IL-6, test with NBT rate. Statistical calculations were performed in R Foundation for Statistical Computing. Results. Urticaria with undetermined causal factor happend more often, urticaria with allergic or pseudo-allergic genesis, as well as parasitic infections were established only in 17% of patients, family allergic history was found in a third of patients, in 41% of children acute urticaria proceeded with angioedema, in 9,3% of children urticaria acquired chronic course. Statistically significant increase of CD14+CD284+, decrease of CD14+CD289+, high concentrations of lactoferrin, decrease of natural killers, increase total serum IgE level in the half of the patients, low microbicidal activity with a decrease in adaptation reserves and reduced levels of IL-4, increased the content of IFN-y were found. Conclusion. The revealed changes indicate an imbalance in innate immunity, the launch of the cytokine cascade of inflammation, which is accompanied by further activation of the regulatory components of the inflammatory process in children suffering from acute urticaria.

scholarly journals CYTOKINE PROFILE AND IMPORTANCE OF ITS ASSESSMENT IN FORECASTING THE COURSE OF DUST DISEASES OF THE LUNGS

2017 ◽  
Vol 2 (1) ◽  
pp. 61-64
Author(s):  
SA A Babanov ◽  
DS S Budash
Keyword(s):  
Lung Diseases ◽  
Secondary Prophylaxis ◽  
Cytokine Profile ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Complete Physical Examination ◽  
Occupational Lung Diseases

Aim - study of cellular immunity and cytokine profile in dust lung diseases caused by exposure to aerosols of various degrees of fibrogenity; assessment of the role of immunological changes in the pathogenesis of occupational lung diseases of dust aetiology. Materials and methods. 161 patients from the contact group with chronic dust bronchitis, silicosis and pneumoconiosis due to exposure to welding aerosols were examined. The control group included 60 people who did not have contact with occupational hazards at work and who were declared healthy according to complete physical examination. The diagnosis was made on the basis of professional anamnesis, sanitary-hygienic characteristics of working conditions (work in contact with aerosols of various degrees of fibrogenity), and a complete clinical and instrumental examination of patients. The levels of cytokines IL-1a, IL-ф, IL-4, IL-8, TNF-a, IFN-y and fibronectin in the blood serum were determined using enzyme-linked immunosorbent assay. Results. The specific features of the course of dust diseases of the lungs were revealed (identified features of the cytokine profile, hypercytokinaemia of a significant number of cytokines), which allows us to characterize the occurrence and the progression of dust diseases of the lungs. Conclusion. Determination of the cytokine profile allows us not only to improve the quality of early detection, but also to optimize the strategy of primary and secondary prophylaxis of this disease, to predict the course of the disease, and to reduce the number of disabling forms.

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