scholarly journals Crioconservación de yemas de microtubérculos de papa Solanum tuberosum ssp. andigena mediante desecado de tejidos

2009 ◽  
Vol 9 (2) ◽  
pp. 37
Author(s):  
Ángela Liliana Rivera Calderón ◽  
Raúl Iván Valbuena Benavides ◽  
Rigoberto Hidalgo Hidalgo ◽  
José Dílmer Moreno Mendoza
Keyword(s):  
Solanum Tuberosum ◽  
Experimental Design ◽  
Environmental Factors ◽  
Gibberellic Acid ◽  
Liquid Nitrogen ◽  
Activated Charcoal ◽  
Culture Medium ◽  
Main Step ◽  
Randomized Experimental Design ◽  
Tetrazolium Test

<p class="Corpoica"><span lang="ES-CO">En la Corporación de Investigación Agropecuaria Corpoica, C.I. Tibaitatá, se evaluó la crioconservación de yemas de microtubérculos en dos accesiones de papa Solanum tuberosum ssp. andigena de la Colección Central Colombiana: C.C.C. 4318 (carriza) y C.C.C. 4981 (guata negra) mediante la metodología del desecado de yemas. Esta metodología tuvo dos etapas: la preliminar o de ajuste y la principal. En la principal, el secado se hizo con flujo de aire estéril durante 30 minutos, crioconservación en nitrógeno líquido a -196°C durante un día, descongelamiento en baño maría a 37°C durante 3 minutos y recuperación en medio de cultivo (100% MS + 8% sacarosa + 2 g/L carbón activado) durante siete días en oscuridad; luego, el medio de cultivo se cambió (100% MS + 0,04 mg/L kinetina + 0,1mg/L ácido giberélico). El diseño experimental fue al azar: dos accesiones, cuatro tratamientos, diez repeticiones y diez yemas. </span>Se evaluó sobrevivencia a 7, 14 y 30 días. <span lang="ES-CO">Paralelamente se adaptó la prueba de tetrazolio en semillas al 0,5% a 25°C durante 24 horas, y se evaluó a los 14 y 30 días; en esta prueba se dificultó la evaluación de la sobrevivencia de las yemas posiblemente por efecto del medio de cultivo y los factores ambientales; el resultado final fue pérdida de sobrevivencia a los 30 días, posiblemente porque las condiciones de recuperación no fueron aptas. </span></p><p class="Corpoica"><span lang="ES-CO"> </span></p><p class="Corpoica"><strong><span lang="EN-US">Cryoconservation of buds of potato’s microtubers Solanum tuberosum ssp. andigena by means of drying tissues</span></strong></p><p class="Corpoica">At the Corporación de Investigación Agropecuaria Corpoica, C.I. Tibaitatá, the cryoconservation of microtubers buds was evaluated in two potato accessions of Solanum tuberosum ssp. andigena, C.C.C. 4318 (carriza) and C.C.C. 4981 (guata negra), from the Colombian Central Collection by using the dried bud methodology. This methodology had two steps, the preliminary one or adjustment and the main one. In the main step, the tissue drying was made with sterile air flow for 30 minutes, cryoconservation in liquid nitrogen at -196°C during 1 day, water bath thawing at 37°C for 3 minutes and recuperation culture medium (100% MS + 8% sucrose + 2 g/L activated charcoal) during 7 days in dark, then the cultured medium was changed (100% MS + 0.04 mg/L kinetin + 0.1mg/L gibberellic acid). A completely randomized experimental design with 2 accessions, 4 treatments, 10 replications and 10 buds, was used. The survival was evaluated at 7, 14 and 30 days. At the same time, the tetrazolium test on seeds was adapted at 0.5% a 25°C for 24 hours, and evaluated after 14 and 30 days. In this bioassay, the survival evaluation of the buds was difficult possibly due to the effect of culture medium and environmental factors. The final result was loss of survival at 30 days, probably because the recovery conditions were not suitable. </p>

scholarly journals Polpa de banana e sacarose no desenvolvimento in vitro de orquídeas

2021 ◽  
Vol 4 (2) ◽  
pp. 70-77
Author(s):  
Eliane Lima de = Aquino ◽  
◽  
Tarcísio Rangel do Couto ◽  
João Sebastião de Paula Araújo ◽  
◽  
...  
Keyword(s):  
Experimental Design ◽  
Seedling Growth ◽  
Culture Medium ◽  
In Vitro Cultivation ◽  
Fresh Weight ◽  
Randomized Experimental Design ◽  
Number Of Leaves ◽  
Weight Number

The objetive of this study was to evaluate the effects of adding two types of banana pulp, combined with varying concentrations of sacarose on the growth of Cattleya sp. plantlets. Hybrid LCTV-01 seedlings (Cattleya labiata rubra x Cattleya labiata semi alba) made to germinate in vitro were inoculated in an MS culture medium with half the concentration of nutrients and supplemented with 60 g.L-1 'maçã' or 'terra' banana pulp in addition to different concentrations of sacarose (10, 20 and 30 g.L-1. The entirely randomized experimental design was chosen, implemented in seven treatments, ten repetitions and eight seedlings per repetition. After 160 days of in vitro cultivation, variables of fresh weight, number of leaves, number of roots and length of the longest root were evaluated. It was found that the addition of banana pulp of any of the analyzed cultivars promoted better seedling growth. Additionally, the 20 g.L-1 sacarose concentration yielded better results for the analyzed variables.

scholarly journals Uso de diferentes pontas de pulverização no controle de doenças no trigo

2019 ◽  
Vol 40 ◽  
pp. 11
Author(s):  
Alicia Baumhardt Dorneles ◽  
Eduardo Leonel Bottega ◽  
Zanandra Boff de Oliveira ◽  
Alberto Eduardo Knies ◽  
Clarissa Moraes da Silva ◽  
...  
Keyword(s):  
Experimental Design ◽  
Environmental Factors ◽  
Chemical Control ◽  
Rio Grande ◽  
Control Plot ◽  
State University ◽  
Rio Grande Do Sul ◽  
Randomized Experimental Design ◽  
Management Techniques ◽  
Grain Contamination

Unstable environmental factors contribute to the incidence of diseases and pests, causing reduced yield and grain contamination, especially the attack by Giberela (Fusarium graminerum). In this scenario, appropriate management techniques are needed. Strategies such as choosing the suitability of the spray tip for chemical control are indispensable. The aim of this study was to use four spray tips: CVI 11002, ADI ISO 11002, TVI ISO 8002 and ATR 8002. The experiment was conducted in the experimental area of The State University of Rio Grande do Sul, in the district of Três Vendas, in Cachoeira do Sul-RS. The cultivar used was TBIO SELETO. The fungicide used was Nativo®. A completely randomized experimental design with 4 replicates and a control plot was adopted. The following parameters were evaluated: weight of one thousand grains (PMG, %), hectoliter weight (PH; kg hL-1) and yield (PRD; kg ha-1). The variance was tested by applying the Snedcor F test (p<0.05) and the means were compared by the Tukey's test (p<0.05). The weight of one thousand grains, hectoliter weight and yield were not influenced by the use of different spray tips adopted in the wheat phytosanitary control.

scholarly journals In Vitro Adventitious Rooting of Carrizo Citrange Microshoots

HortScience ◽  
2010 ◽  
Vol 45 (6) ◽  
pp. 988-990 ◽  
Author(s):  
Almudena Montoliu ◽  
Aurelio Gómez-Cadenas ◽  
Rosa M. Pérez-Clemente
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
Activated Charcoal ◽  
Culture Medium ◽  
Basal Medium ◽  
Poncirus Trifoliata ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Carrizo Citrange

The objective of this work was to develop an efficient in vitro rooting protocol for one of the most commercially used citrus rootstocks in Spain, Carrizo citrange (Citrus sinensis L. Osbeck × Poncirus trifoliata L. Raf.). Single-node cuttings taken from greenhouse-grown plants were cultured in petri dishes containing basal Murashige and Skoog medium. Shoots from nodal stem segments were excised and cultured in a multiplication medium (basal medium supplemented with 1.8 μM 6-benzylaminopurine) to promote the development of axillary buds. Individual shoots (15 mm long) were treated with different hormones at several concentrations for root induction evaluations. The addition of activated charcoal (AC) to the culture medium was also explored. The addition of auxins to the culture medium enhanced rooting percentage. Optimal results were obtained when 1-naphthalene acetic acid (10.8 μM) and gibberellic acid (0.3 μM) were added to the culture medium. The addition of AC to the rooting medium resulted in negative effects on the percentage of rooted shoots but had a positive effect on number of roots per rooted shoot. Chemical names used: activated charcoal (AC); 6-benzylaminopurine (BA); 1-naphthalene acetic acid (NAA); gibberellic acid (GA3); indole-3-butyric acid (IBA)

scholarly journals In vitro growth of Oncidium sphacelatum (Orchidaceae) in different concentrations of sucrose and nutrients

2019 ◽  
pp. 20-35
Author(s):  
Ana Luisa Navarrete-Valencia ◽  
Leobarda Guadalupe Ramírez-Guerrero ◽  
Ana Luisa Sánchez-Monteón ◽  
Víctor Manuel Jiménez-Meza
Keyword(s):  
Experimental Design ◽  
Nutrient Medium ◽  
In Vitro Propagation ◽  
Nutrient Concentration ◽  
Culture Medium ◽  
Nutrient Concentrations ◽  
In Vitro Growth ◽  
Randomized Experimental Design ◽  
Number Of Leaves

The objective of the present work was to offer an in vitro propagation alternative for the Oncidium sphacelatum orchid through the evaluation of the response to the crop in different concentrations of sucrose and the nutrient medium of Murashige and Skoog. Initially the seeds were sown in this culture medium at 100% of their concentration with the addition of 3.0% sucrose, once formed the protocorms the response to the application of different sucrose concentrations was evaluated (3.0, 2.25, 1.5, 0.75 and 0%) and of the nutrients of the culture medium (100, 75, 50, 25 and 0%) under a completely randomized experimental design of eight treatments with four repetitions. The analysis of variance showed statistical differences between treatments for the formation of buds in the protocorms, number of leaves per bud, plant height, root length and formation of pseudobulbs in the shoots. The highest outbreak formation in the protoorms was observed in treatments with 3.0% sucrose and nutrient concentrations at 50 or 25%. For the growth of the leaves and the root the concentration of 3.0% of sucrose was required combined with the concentrations of nutrients at 25, 50 and 100%. The formation of pseudobulbs in the outbreaks only occurred in the treatment with 3.0% sucrose combined with the 25% nutrient concentration.

HVEM Cryomicroscopy of Frozen Hydrated PTK1 Cells

1990 ◽  
Vol 48 (1) ◽  
pp. 500-501
Author(s):  
E.T. O’Toole ◽  
G.P. Wray ◽  
J.R. Kremer ◽  
J.R. Mcintosh
Keyword(s):  
Liquid Nitrogen ◽  
Phase Contrast ◽  
Culture Medium ◽  
Low Dose ◽  
Ammonium Acetate ◽  
Freezing Point ◽  
Voltage Electron ◽  
Cold Stage ◽  
Humidity Chamber ◽  
Coated Carbon

Ultrarapid freezing and cryomicroscopy of frozen hydrated material makes it possible to visualize samples that have never been exposed to chemical fixatives, dehydration, or stains. In principle, freezing and cryoimaging methods avoid artifacts associated with chemical fixation and processing and allow one to visualize the specimen in a condition that is close to its native state. Here we describe a way to use a high voltage electron microscope (HVEM) for the cryoimaging of frozen hydrated PTK1 cells.PTK1 cells were cultured on formvar-coated, carbon stabilized gold grids. After three days in culture, the grids were removed from the culture medium and blotted in a humidity chamber at 35° C. In some instances, the grids were rinsed briefly in 0.16 M ammonium acetate buffer (pH 7.2) prior to blotting. After blotting, the grids were transferred to a plunging apparatus and plunged into liquid ethane held directly above its freezing point. The plunging apparatus consists of a vertical slide rail that guides the fall of a mounted pair of forceps that clamp the specimen. The forceps are surrounded by a plexiglass humidity chamber mounted over a dewar of liquid nitrogen containing an ethane chamber. After freezing, the samples were transferred to liquid nitrogen and viewed in a JEOL JEM 1000 equipped with a top entry cold stage designed and built by Mr. George Wray (Univ. Colorado). The samples were routinely exposed to electron doses of 1 e/Å2/sec, and viewed at a temperature of −150° C. A GATAN video system was used to enhance contrast and to estimate the correct amount of underfocus needed to obtain phase contrast at various magnifications. Low dose micrographs were taken using two second exposures of Kodak 4463 film. The state of the solid water in the specimen was determined by diffraction using a 30/μm field limiting aperture and a camera length of 1 meter.

PENGARUH KONSENTRASI RAGI Saccharomyces cerevisiae DAN LAMA FERMENTASI TERHADAP KUALITAS CUKA TOMI-TOMI (Flacourtia inermis)

2015 ◽  
Vol 4 (2) ◽  
pp. 50-55
Author(s):  
Sandra J Nendissa ◽  
Rachel Breemer ◽  
Nikholaus Melamas
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Experimental Design ◽  
Total Dissolved Solids ◽  
Total Sugar ◽  
Dissolved Solids ◽  
Randomized Experimental Design ◽  
Two Factors ◽  
Four Levels

This objectives of this research were both to study and determine the best level of concentration of yeast Saccharomyces cereviseae and period of fermentation on the quality of tomi-tomi vinegar (Flacourtia inermis). A completely randomized experimental design with two factors of treatment was applied in this research. The first factor was concentration of yeast S. cereviseae having four levels of tretament, i.e.: without the addition of yeast 0.5, 1 and 1.5 g yeast. The second factor was period fermentation with 1, 2, 3, 4, and 5 weeks. The result indicated that the concentration of yeast S. cereviseae 1.5 g and period fermentation 5 week produced a good tomi-tomi vinegar with total acids 51.22%, total dissolved solids 8.35, total sugar 8.07% and pH 5.40.

scholarly journals Use of chemometric techniques to design a microbiological method for sulfonamide detection in milk

2013 ◽  
Vol 31 (No. 6) ◽  
pp. 627-632 ◽  
Author(s):  
O.G. Nagel ◽  
M.P. Molina ◽  
R.L. Althaus
Keyword(s):  
Experimental Design ◽  
Logistic Model ◽  
Logistic Regression Model ◽  
Culture Medium ◽  
Spore Concentration ◽  
Microbiological Method ◽  
Burman Design ◽  
Detection Capabilities ◽  
Sulfonamide Residues ◽  
Plackett Burman Design

We proposed an experimental design of a microbial bioassay of dichotomous response (positive or negative) using Bacillus subtilis BGA for the detection of sulfonamide residues. In the first stage, the bioassay response time was reduced to 6 h by increasing the spore concentration of B. subtilis. Then, the effects of spore, indicator, trimethoprim (TMP) concentration, and volume of the culture medium were examined with a Plackett Burman design (2<sup>4-1</sup>). Finally, the effect of TMP concentration on the method detection capabilities and specificity was analysed using a logistic model with interaction. The detection capabilities of sulfonamides in milk are close to the MRLs when using 500 mg/l of TMP in the culture medium of the bioassay. It is concluded that the experimental design techniques and a logistic regression model can be used to design successfully a dichotomous response bioassay.

scholarly journals Cultivation of gerbera irrigated with treated domestic effluents

2010 ◽  
Vol 14 (6) ◽  
pp. 582-588 ◽  
Author(s):  
Lisânea M. O. Damasceno ◽  
Aderson S. de Andrade Júnior ◽  
Hans R. Gheyi
Keyword(s):  
High Temperature ◽  
Experimental Design ◽  
Relative Humidity ◽  
Plant Development ◽  
Leaf Growth ◽  
Treated Wastewater ◽  
Nutritional Requirements ◽  
Randomized Experimental Design ◽  
Growth Production ◽  
Domestic Effluents

This paper evaluates the nutritional impact on growth, production and quality variables of gerbera crop when fertigated with treated domestic effluents. An experiment was carried out in greenhouse at the Embrapa Meio-Norte in Teresina, in the State of Piauí, Brazil, from July to October 2007. A completely randomized experimental design with five treatments and five replications was adopted. The treatments investigated were T1 - 100% of water and nutritional requirements of crop were met with chemical fertigation (N e K2 O); T2 - 25% volume of water through fertigation and 75% treated wastewater effluents (TWE); T3: 50% volume of water through fertigation and 50% TWE; T4: 75% volume of water with fertigation and 25% TWE; and T5 - 100% volume of water supplied through TWE. Leaf growth and plant development were favored by the application of 50% fertigation and 50% TWE (T3). As for commercial requirements, the best results for number of flowers were obtained with T4. However, concerning quality, T2 produced flowers in July and August with longer stems, but in the months of September and October, no treatment achieved this standard due to high temperature and low relative humidity of the air in the region.

scholarly journals Microbiota indigenous milk, mesophilic lipolytic and proteolytic colonial cheese matured, produced at different times of the year

2017 ◽  
Vol 18 (4) ◽  
pp. 549-559
Author(s):  
Elisângela Borsoi Pereira ◽  
Magali Soares dos Santos Pozza ◽  
Paula Martins Olivo ◽  
Osmar Dalla Santa ◽  
Suzana da Cruz Pires ◽  
...  
Keyword(s):  
Weight Loss ◽  
Lactic Acid ◽  
Experimental Design ◽  
Relative Humidity ◽  
Water Activity ◽  
Cultural Importance ◽  
The Third ◽  
Randomized Experimental Design ◽  
Double Factorial ◽  
Production Period

SUMMARY Cheese is the oldest form of preserving milk nutrients having nutritional, economic and cultural importance. The objective of this study was to identify the best time of the year for production, and period, in months, for maturation of traditional colonial cheese, through analysis of water activity, weight loss and counts of lactic acid, mesophilic microorganisms—proteolytic and lipolytic. Records of temperature and relative humidity (RH) were maintained. A completely randomized experimental design was used in a double factorial scheme, considering production periods and maturation times. For all production periods evaluated, there was a significant reduction in the periods for water activity values. The counts of lactic acid bacteria ranged from 104 to 109 CFU/g. There was also stability in the number of colonies for lipolytic mesophilic microorganisms, until the third month of maturation. Low counts of proteolytic mesophiles were observed for the samples produced in May and June (5.70 and 5.53 log), respectively. The production period for the months of May and June corresponding to RH of 80% and average temperatures of 15°C were the most effective for production. Due to the presence of Listeria, it is recommended to respect the minimum time of 60 days of maturation for commercialization.

scholarly journals Collection season and auxin treatment in the propagation by cuttings of mandarin hybrids

2016 ◽  
Vol 46 (2) ◽  
pp. 215-221 ◽  
Author(s):  
Andrés Iván Prato Sarmiento ◽  
Paulo Vitor Dutra de Souza ◽  
Sergio Francisco Schwarz
Keyword(s):  
Experimental Design ◽  
Vegetative Growth ◽  
Late Spring ◽  
Indolebutyric Acid ◽  
Rooted Cuttings ◽  
Exogenous Application ◽  
Auxin Treatment ◽  
Randomized Experimental Design

ABSTRACT Propagation by cuttings is an alternative for obtaining citrus rootstocks. The exogenous application of indolebutyric acid (IBA), cutting collection season and genotypes used may affect the rooting and vegetative growth of citrus rootstocks cuttings. Two experiments were conducted to study the influence of these factors. In the first one, semi-hardwood cuttings from the 'Sunki' mandarin hybrids H49 and H77 were collected in the fall and late spring of 2013 and treated with IBA (0 mg L-1, 1,500 mg L-1 and 3,000 mg L-1). For each collection season, a 2 x 3 (two genotypes x three IBA concentrations) factorial scheme was adopted, in a randomized blocks design. In the second experiment, the development of cuttings that rooted in the late spring was evaluated until grafting. In this case, a completely randomized experimental design was adopted, with the hybrids H49 and H77. The IBA treatments positively influenced rooting and number of roots only in the fall collection, peaking at 23.3 % of rooted cuttings. In the late spring collection, rooting was close to 100 %, with the IBA treatment being unnecessary. Around 50 % of cuttings from the 'Sunki' mandarin hybrids were ready for grafting at 14 months after cutting.

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