scholarly journals Synergistic Effect of Two Type Cellulase Immobilized on Chitosan Microparticle as Biocatalyst for Coconut Husk Hydrolysis

2019 ◽  
Vol 19 (2) ◽  
pp. 495
Author(s):  
Afan Hamzah ◽  
Lidya Lorenta Sitompul ◽  
Irma Nurhanifah Fenda Putri ◽  
Soeprijanto Soeprijanto ◽  
Arief Widjaja
Keyword(s):  
Aspergillus Niger ◽  
Synergistic Effect ◽  
Reducing Sugar ◽  
Free Enzyme ◽  
Coconut Husk ◽  
Enzyme Coupling ◽  
Effective Combination ◽  
High Enzyme ◽  
Immobilized Cellulase ◽  
Very High

The effectivity of employing two types of cellulases from Aspergillus niger and Trichoderma resei covalently immobilized on chitosan microparticle was investigated. Reducing sugar from CMC yielded by immobilized cellulase from T. resei alone and A. niger alone was 0.316 g/L and 0.244 g/L, respectively. Simultaneous use of both cellulases shows a significant increase of reducing sugar produced to 1.020 g/L. The effective combination of this two types of cellulases also occurred when coconut husk was used as substrate. A very high enzyme coupling of 92.06% compared to free enzyme was obtained in the immobilization. Addition of GDA not only increased enzyme coupling to 100% but also improved sugar produced. Immobilized cellulase was successfully maintained its activity until 5 cycles

scholarly journals EFFECT OF MUCUNA COCHINCHINENSIS EXTRACTS ON EFFLUX PUMP IN FUNGAL STRAINS

2020 ◽  
pp. 94-96
Author(s):  
NATARAJAN K. ◽  
KUMARESH JAWAHAR N.
Keyword(s):  
Experimental Data ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Synergistic Effect ◽  
Efflux Pump ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Inhibition Activity ◽  
Fungal Strains ◽  
Efflux Pump Inhibition

Objective: To study the efflux pump inhibition activity of Mucunacochinchinensis extracts Methods: Sensitivity assay was performed by Kirby Bauer technique. Effluxing ability of microbe was done by accretion, accumulation and efflux pump assays. Results: Resistant fungal strains were subjected to efflux ability studies. Maximum effluxing ability was determined from the experimental data and it was found to be 105 min and 75 min by Candida albicans for methanolic extract of M. cochinchinenesis (MMC) and ethanolic extract of M. cochinchinensis (EMC) respectively and 45 min and 105 min by Aspergillus niger for MMC and EMC respectively. Profound synergistic effect of inhibition was observed with combined MMC and standard Clotrimoxazole when compared to the standard alone; against C. albicansindicates the modulating efficacy of MMC. Conclusion: M. cochinchinensis extracts showed efflux pump modulating activity.

In Situ Saccharification of Cellulose in Mild Ionic Liquid Using Sodium Alginate Immobilized Cellulase

2013 ◽  
Vol 361-363 ◽  
pp. 339-342 ◽  
Author(s):  
Juan Juan Fei ◽  
Qiang Li ◽  
Yuan Yuan Feng ◽  
Geng Sheng Ji ◽  
Xu Ding Gu ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Sodium Alginate ◽  
Reducing Sugars ◽  
Enzymatic Saccharification ◽  
Reducing Sugar ◽  
Candida Shehatae ◽  
Ionic Liquid Treatment ◽  
Immobilized Cellulase

The work is to select biocompatible ionic liquid (IL) toward in situ saccharification of cellulose and investigating the effect of enzymatic saccharification with sodium alginate immobilized cellulase. The [Mmi [DM was selected for the ionic liquid treatment improved the yield of reducing sugars and the hydrolyzates could be efficiently fermented to ethanol. The yield of reducing sugar is 89.54% for 48h. In the in situ saccharification process, the yield of sugars were 84.52% and 86.72% with immobilized cellulase and free cellulase saccharification for 48h. Then the hydrolyzates could be fermented to ethanol withCandida shehatae. The yield of ethanol was 0.42g/g glucose within 24h.

Biological control of Aspergillus niger, the cause of Black-rot disease of Allium cepa L. (onion), by Penicillium species

2012 ◽  
Vol 29 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Ibatsam Khokhar ◽  
Muhammad Saleem Haider ◽  
Irum Mukhtar ◽  
Sobia Mushtaq
Keyword(s):  
Biological Control ◽  
Aspergillus Niger ◽  
Agar Plate ◽  
Antagonistic Effect ◽  
Black Rot ◽  
Dual Culture ◽  
Penicillium Species ◽  
Allium Cepa L ◽  
Rot Disease ◽  
Very High

Abstract The purpose of the study was to explore the control of the onion black rot pathogen, Aspergillus niger, with Penicillium species as biological control agents. Fourteen Penicillium species were isolated from the rhizosphere of different plants. In dual culture agar plate assays, these isolates showed very high antagonistic effects on the growth of A. niger mycelium. Penicillium roqueforti and P. viridicatum greatly inhibited the growth of A. niger by 66% and 60%, respectively, followed by P. bilaii (57%) and P. olsonii (53%). However, it was also observed that the Penicillium species completely overgrew the A. niger colony. The study revealed that some species of the genus Penicillium possessed a high antagonistic effect on the onion black rot pathogen.

Synergistic effect of migrating Ascaris larvae and Escherichia coli in piglets

1989 ◽  
Vol 63 (1) ◽  
pp. 19-24 ◽  
Author(s):  
S. O. Adedeji ◽  
E. O. Ogunba ◽  
O. O. Dipeolu
Keyword(s):  
Escherichia Coli ◽  
Synergistic Effect ◽  
Intestinal Flora ◽  
Internal Organs ◽  
Large White ◽  
E Coli ◽  
Large White Breed ◽  
Loss Of Weight ◽  
White Breed ◽  
Very High

ABSTRACTThe effect of intestinal flora on the establishment, development and pathogenicity of Ascaris suum larvae in piglets (Large White breed) was investigated. The infected piglets with Ascaris and Escherichia coli showed signs of pneumonia, cough with respiratory difficulties initially even though these moderated with time. They lost appetite and showed signs of unthriftiness with loss of weight. The packed cell volume was normal but the differential leucocyte counts of the pigs infected with Ascaris larvae and bacteria had high neutrophils, unlike the very high lymphocyte count observed in piglets with ascarids only. The piglets had generalized serous atrophy of body fat. The pericardial and perirenal fats were gelatinous. There was a firm and nodular grey and red hepatization with abscess pockets in the intermediate and anterior one third of the diaphragmatic lobes of the lungs. The liver contained greyish-white and depressed focus immediately dorsal to the area of attachment to the gall bladder with multifocal areas. There was no significant gross lesion in the control animals. Cultural and microscopic examinations of some internal organs of the infected animals showed that bacteria were carried to the lungs by the migrating Ascaris larvae. The combined synergistic effect of Ascaris larvae and E. coli was also investigated and it was concluded that the two agents (A. suum larvae and E. coli) worked together synergistically.

scholarly journals Fermentasi Produksi Asam Sitrat menggunakan Aspergillus Niger ATCC 16404 dengan Substrat Hidrolisat Cair Limbah Padat Industri Brem

2019 ◽  
Vol 7 (4) ◽  
pp. 615
Author(s):  
Gede Asstaradi Kusuma ◽  
Nyoman Semadi Antara ◽  
Ni Putu Suwariani
Keyword(s):  
Citric Acid ◽  
Experimental Design ◽  
Aspergillus Niger ◽  
Acid Production ◽  
Block Design ◽  
Reducing Sugar ◽  
Soluble Solids ◽  
Calcium Citrate ◽  
Randomized Block Design ◽  
Static Cultivation

Dregs of solid waste brem industry contained 12 percent of sugar and 10,8 percent of starch. Component contained in the dregs are expected to produce citric acid. The aims of this study were to determine the effect of static cultivation on the production of citric acid from liquid hydrolyzate brem waste by using Aspergillus niger. The experimental design used in this study was a simple randomized block design (RAK), which the static cultivations (0, 1, 2, 3 days) as a treatment. The results showed that the static cultivation treatment significantly affected the level of acidity (pH), total soluble solids, biomass, reducing sugar, calcium citrate, citric acid and yield. The static cultivation for 1 day was the best treatment to produce Ca-citrate which the production level was 3,84 ± 0,05 g/L. By the stoichiometry method the citric acid production could be calculated, which equal to 2,12 ± 0,03 g/L. Keywords: Aspergillus niger, dregs of brem, static cultivation, Ca-citrate, citric acid  

Characterization of the ?-Glucosidase Activity Associated with Immobilized Cellulase of Aspergillus niger

1987 ◽  
Vol 501 (1 Enzyme Engine) ◽  
pp. 503-507 ◽  
Author(s):  
F. ALFANI ◽  
L. CANTARELLA ◽  
A. GALLIFUOCO ◽  
L. PEZZULLO ◽  
V. SCARDI ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Glucosidase Activity ◽  
Immobilized Cellulase

scholarly journals A novel strain of Aspergillus niger producing a cocktail of hydrolytic depolymerising enzymes for the production of second generation biofuels

BioResources ◽  
2010 ◽  
Vol 6 (1) ◽  
pp. 552-569 ◽  
Author(s):  
Namita Bansal ◽  
Rupinder Tewari ◽  
Jugal Kishore Gupta ◽  
Raman Soni ◽  
Sanjeev Kumar Soni
Keyword(s):  
Aspergillus Niger ◽  
Second Generation ◽  
Hydrolytic Enzymes ◽  
Reducing Sugar ◽  
Soil Samples ◽  
Domestic Waste ◽  
Second Generation Biofuels ◽  
Pectinolytic Enzymes ◽  
Enzyme Cocktail ◽  
Conversion Efficiencies

The screening and isolation of fungi producing a cocktail of hydrolytic enzymes was studied. Among the various isolates obtained from different soil samples, a strain NS-2 was selected. The phylogenetic analysis of this strain showed highest homology (99%) with Aspergillus niger. It was capable of producing cellulolytic, hemicellulolytic, amylolytic, and pectinolytic enzymes in appreciable titers on wheat bran based liquid and solid state media. The mixture of enzymes produced by this organism could effectively hydrolyze various domestic waste residues, revealing conversion efficiencies of 89 to 92% and produced high reducing sugar yields of 0.48 to 0.66 g/g of dry residue. This enzyme cocktail could potentially find a significant application in the conversion of agricultural and other waste residues having cellulose, hemicellulose, starch, and pectin as carbohydrates to produce simpler sugars which can be fermented for the production of second generation biofuels.

Kinetics of Aspergillus niger Cellulase Inhibition by Reducing Sugar Produced by the Hydrolysis of Carboxymethylcellulose

2007 ◽  
Vol 5 (1) ◽  
Author(s):  
Sulaiman Al-Zuhair ◽  
Yu Ling Fan ◽  
Kit Soon Chui ◽  
Sarmad Rizwan
Keyword(s):  
Experimental Data ◽  
Aspergillus Niger ◽  
Competitive Inhibition ◽  
Batch Reactor ◽  
Product Inhibition ◽  
Reducing Sugar ◽  
Wood Shavings ◽  
Initial Substrate ◽  
Competitive Model ◽  
Hydrolysis Of

Hydrolysis of two types of cellulose, namely, totally amorphous Carboxymethylcellulose (CMC) and highly crystalline wood shavings, by Aspergillus niger cellulase was studied in a stirred batch reactor at 50oC. Two kinetic models were devised to predict the rate of reducing sugar production assuming competitive and non-competitive product inhibition of the enzyme. The applicability of the models was tested by comparing experimental results with those predicted by the models. Both models followed the experimental data fairly well, giving standard deviation of 13.3 and 7.9 for the competitive and the non-competitive inhibition models, respectively. However the non-competitive inhibition model showed more realistic behaviour than the non-competitive model and represented better the experimental data. The significance of crystallinity was determined by comparing the production of reducing sugar from wood shavings to the production from CMC. At same initial substrate concentration, it was found that the production of reducing sugar ceased at much shorter times for wood shavings in comparison to that for CMC.

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