Difluoroboron Curcumin Complex: A Study on Determination of Acidity Constants and Quantitative Analysis of Arsenic(III)

In this study, the complex of difluoroboron, curcumin (BF2-Cur), has been synthesized and characterized via the combination of Boron trifluoride-diethyl etherate ((C2H5)2OBF3) and curcumin. However, the new dissociation constants, pKa1 and pKa2 of the BF2-Cur complex, have been indicted by the values of 8.44 ± 0.16 and 9.76 ± 0.13, respectively. On the other hand, the reagent was also used to determine As(III) in aqueous solutions by UV–Vis spectrophotometry. As a result, the method was validated for accuracy, precision, linearity, and sensitivity, and the linear range was from 1.0 to 25.0 µmol/L, with the linear regression, A = 0.0027 C + 0.0106, correlation coefficient R2 = 0.9969. Besides, the limit of detection (LOD) and limit of quantification (LOQ) were determined as 0.83 and 2.10 µmol/L, respectively. Thus, the developed method is successfully used for quantitative analysis of total arsenic in wastewater by reducing As(V) to As(III), then determining As(III) with high accuracy results.

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Fast and sensitive method for phosphorus determination in dairy products

Journal of Consumer Protection and Food Safety ◽

10.1007/s00003-021-01329-x ◽

2021 ◽

Author(s):

Anna Gliszczyńska-Świgło ◽

Iga Rybicka

Keyword(s):

Quantitative Analysis ◽

Dairy Products ◽

Phosphorus Content ◽

Limit Of Detection ◽

Sustainable Chemistry ◽

Limit Of Quantification ◽

Molybdenum Blue ◽

Phosphorus Determination ◽

Sensitive Method

AbstractThe spectrophotometric molybdenum blue method for phosphorus determination was adapted to a multiwell plate format. The method was sensitive and allowed for the simultaneous determination of phosphorus in many samples. It was cheap and eco-friendly due to application of small volumes of reagents and, therefore, it meets the requirements for “green” or sustainable chemistry. The method’s limit of detection (LOD) is 0.37 μg/mL and its limit of quantification (LOQ) is 1.13 μg/mL. Its linearity is up to 30 μg of phosphorus/mL. The method was applied for the determination of phosphorus in 65 dairy products (yogurts, yogurt drinks, buttermilks, kefirs and homogenized cheeses) of strawberry, peach, forest fruits, vanilla and other flavours. The phosphorus content was 143–226 mg/100 g in flavoured yogurts, 78–204 mg/100 g in yogurt drinks, 89–218 mg/100 g in kefirs, around 195 mg/100 g in buttermilks, and 165–277 mg/100 g in homogenized cheeses. The presented method can be used in the routine quantitative analysis of the total phosphorus content in dairy products.

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Spectrophotometeric Determination of Trifluoperazine HCL in Pure Forms and Pharmaceutical Preperations

International Journal of Pharmaceutical and Clinical Research ◽

10.25258/ijpcr.v9i5.8593 ◽

2017 ◽

Vol 9 (5) ◽

Author(s):

Mohammad Hamzah Hamzah ◽

Rawa M M Taqi ◽

Muna M. Hasan ◽

Raid J. M. Al-Timimi

Keyword(s):

Standard Method ◽

Limit Of Detection ◽

Molar Absorptivity ◽

Dosage Forms ◽

Oxidizing Agent ◽

Optimum Conditions ◽

Limit Of Quantification ◽

Cerium Ion ◽

Blank Solution

A simple and accurate spectrophotometric method for the determination of Trifluoperazine HCl in pure and dosage forms was developed. The method is based on the reaction between Trifluoperazine HCl and p-chloroaniline in the presence of cerium ion as oxidizing agent which lead to the formation of violate color product that absorbed at a maximum wavelength 570nm while the blank solution was pink. Under the optimum conditions a linear relationship between the intensity and concentration of TRF in the range 4-50μg/ml was obtained . The molar absorptivity 3.74×103 L.mol-1.cm-1 , Limit of detection (2.21μg/ml), while limit of quantification was 7.39μg/ml. The proposed analytical method was compared with standard method using t-test and F-test , the obtained results shows there is no significant differences between proposed method and standard method. Based on that the proposed method can be used as an alternative method for the determination of TRF in pure and dosage forms.

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A novel LC-MS method development and validation for the determination of phenyl vinyl sulfone in eletriptan hydrobromide

Future Journal of Pharmaceutical Sciences ◽

10.1186/s43094-020-00175-2 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Indhu Priya Mabbu ◽

G. Sumathi ◽

N. Devanna

Keyword(s):

Method Development ◽

Limit Of Detection ◽

Vinyl Sulfone ◽

Limit Of Quantification ◽

Relative Standard ◽

Pressure Chemical Ionization ◽

Pressure Chemical ◽

Development And Validation ◽

Phenyl Vinyl

Abstract Background The aim of the present method is to develop and validate a specific, sensitive, precise, and accurate liquid chromatography-mass spectrometry (LC-MS) method for the estimation of the phenyl vinyl sulfone in the eletriptan hydrobromide. The effective separation of the phenyl vinyl sulfone was achieved by the Symmetry C18 (50 × 4.6 mm, 3.5 μm) column and a mobile phase composition of 0.1%v/v ammonia buffer to methanol (5:95 v/v), using 0.45 ml/min flow rate and 20 μl of injection volume, with methanol used as diluent. The phenyl vinyl sulfone was monitored on atomic pressure chemical ionization mode mass spectrometer with positive polarity mode. Results The retention time of phenyl vinyl sulfone was found at 2.13 min. The limit of detection (LOD) and limit of quantification (LOQ) were observed at 1.43 ppm and 4.77 ppm concentration respectively; the linear range was found in the concentration ranges from 4.77 to 27.00 ppm with regression coefficient of 0.9990 and accuracy in the range of 97.50–102.10%. The percentage relative standard deviation (% RSD) for six replicates said to be injections were less than 10%. Conclusion The proposed method was validated successfully as per ICH guidelines. Hence, this is employed for the determination of phenyl vinyl sulfone in the eletriptan hydrobromide.

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DETERMINATION OF 10-GINGEROL IN INDIAN GINGER BY VALIDATED HPTLC METHOD OF SAMPLES COLLECTED ACROSS SUBCONTINENT OF INDIA

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i12.14953 ◽

2016 ◽

Vol 8 (12) ◽

pp. 190

Author(s):

Kamran Ashraf ◽

Syed Adnan Ali Shah ◽

Mohd Mujeeb

Keyword(s):

Thin Layer ◽

Thin Layer Chromatography ◽

High Performance ◽

Limit Of Detection ◽

Limit Of Quantification ◽

Chromatography Method ◽

Aluminum Plates ◽

Layer Chromatography ◽

Hptlc Method

Objective: A simple, sensitive, precise, and accurate stability indicating HPTLC (high-performance thin-layer chromatography) method for analysis of 10-gingerol in ginger has been developed and validated as perICH guidelines.Methods: The separation was achieved on TLC (thin layer chromatography) aluminum plates pre-coated with silica gel 60F254 using n-hexane: ethyl acetate 55:45 (%, v/v) as a mobile phase. Densitometric analysis was performed at 569 nm.Results: This system was found to have a compact spot of 10-gingerol at RF value of 0.57±0.03. For the proposed procedure, linearity (r2 = 0.998±0.02), limit of detection (18ng/spot), limit of quantification (42 ng/spot), recovery (ranging from 98.35%–100.68%), were found to be satisfactory.Conclusion: Statistical analysis reveals that the content of 10-gingerol in different geographical region varied significantly. The highest and lowest concentration of 10-gingerol in ginger was found to be present in a sample of Patna, Lucknow and Surat respectively which inferred that the variety of ginger found in Patna, Lucknow are much superior to other regions of India.

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Development and Validation of a Rapid RP-HPLC Method for the Estimation of Esmolol Hydrochloride in Bulk and Pharmaceutical Dosage Forms

E-Journal of Chemistry ◽

10.1155/2010/470785 ◽

2010 ◽

Vol 7 (3) ◽

pp. 807-812 ◽

Cited By ~ 2

Author(s):

Vanita Somasekhar ◽

D. Gowri Sankar

Keyword(s):

Limit Of Detection ◽

Hplc Method ◽

Detector Response ◽

Reverse Phase Hplc ◽

Limit Of Quantification ◽

Pharmaceutical Dosage Forms ◽

Rp Hplc ◽

Accuracy And Precision ◽

Development And Validation

A reverse phase HPLC method is described for the determination of esmolol hydrochloride in bulk and injections. Chromatography was carried on a C18column using a mixture of acetonitrile, 0.05 M sodium acetate buffer and glacial acetic acid (35:65:3 v/v/v) as the mobile phase at a flow rate of 1 mL/min with detection at 275 nm. The retention time of the drug was 4.76 min. The detector response was linear in the concentration of 1-50 μg/mL. The limit of detection and limit of quantification was 0.614 and 1.86 μg/mL respectively. The method was validated by determining its sensitivity, linearity, accuracy and precision. The proposed method is simple, economical, fast, accurate and precise and hence can be applied for routine quality control of esmolol hydrochloride in bulk and injections.

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Surface-Enhanced Oxidation and Determination of Isothipendyl Hydrochloride at an Electrochemical Sensing Film Constructed by Multiwalled Carbon Nanotubes

International Journal of Electrochemistry ◽

10.1155/2012/875631 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6

Author(s):

S. N. Prashanth ◽

Shankara S. Kalanur ◽

Nagappa L. Teradal ◽

J. Seetharamappa

Keyword(s):

Limit Of Detection ◽

Biological Fluids ◽

Differential Pulse ◽

Electrochemical Sensing ◽

Good Precision ◽

Multiwalled Carbon ◽

Limit Of Quantification ◽

Surface Enhanced ◽

Ph Range

The electrochemical behavior of isothipendyl hydrochloride (IPH) was investigated at bare and multiwalled-carbon-nanotube modified glassy carbon electrode (MWCNT-GCE). IPH (55 μM) showed two oxidation peaks in Britton-Robinson (BR) buffer of pH 7.0. The oxidation process of IPH was observed to be irreversible over the pH range of 2.5–9.0. The influence of pH, scan rate, and concentration of the drug on anodic peak was studied. A differential pulse voltammetric method with good precision and accuracy was developed for the determination of IPH in pure and biological fluids. The peak current was found to be linearly dependent on the concentration of IPH in the range of 1.25–55 μM. The values of limit of detection and limit of quantification were noticed to be 0.284 and 0.949 μM, respectively.

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Determination of 77 Multiclass Pesticides and Their Metabolitesin Capsicum and Tomato Using GC-MS/MS and LC-MS/MS

Molecules ◽

10.3390/molecules26071837 ◽

2021 ◽

Vol 26 (7) ◽

pp. 1837

Author(s):

Harischandra Naik Rathod ◽

Bheemanna Mallappa ◽

Pallavi Malenahalli Sidramappa ◽

Chandra Sekhara Reddy Vennapusa ◽

Pavankumar Kamin ◽

...

Keyword(s):

Limit Of Detection ◽

Solid Phase ◽

Graphitized Carbon Black ◽

Limit Of Quantification ◽

Gas And Liquid Chromatography ◽

Relative Standard ◽

The Matrix ◽

Liquid Chromatography Tandem Mass ◽

Primary Secondary Amine

A quick, sensitive, and reproducible analytical method for the determination of 77 multiclass pesticides and their metabolites in Capsicum and tomato by gas and liquid chromatography tandem mass spectrometry was standardized and validated. The limit of detection of 0.19 to 10.91 and limit of quantification of 0.63 to 36.34 µg·kg−1 for Capsicum and 0.10 to 9.55 µg·kg−1 (LOD) and 0.35 to 33.43 µg·kg−1 (LOQ) for tomato. The method involves extraction of sample with acetonitrile, purification by dispersive solid phase extraction using primary secondary amine and graphitized carbon black. The recoveries of all pesticides were in the range of 75 to 110% with a relative standard deviation of less than 20%. Similarly, the method precision was evaluated interms of repeatability (RSDr) and reproducibility (RSDwR) by spiking of mixed pesticides standards at 100 µg·kg−1 recorded anRSD of less than 20%. The matrix effect was acceptable and no significant variation was observed in both the matrices except for few pesticides. The estimated measurement uncertainty found acceptable for all the pesticides. This method found suitable for analysis of vegetable samples drawn from market and farm gates.

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ESTIMATION OF TADALAFIL IN HUMAN PLASMA BY SPECTROFLUOROPHOTOMETRY

INDIAN DRUGS ◽

10.53879/id.58.06.11329 ◽

2021 ◽

Vol 58 (06) ◽

pp. 60-63

Author(s):

Sadhana Rajput ◽

◽

Samir Patel ◽

Keyword(s):

Human Plasma ◽

Fluorescence Spectrum ◽

Drug Monitoring ◽

Limit Of Detection ◽

Wave Length ◽

Excitation Wavelength ◽

Limit Of Quantification ◽

Pharmacokinetic Investigation ◽

Satisfactory Recovery

A new, specific, selective, simple, rapid and inexpensive spectrofluorophotometric method has been developed for the determination of tadalafil in spiked human plasma. The fluorescence spectrum of tadalafil in 0.1M methanolic sulphuric acid showed excitation wavelength at 315 nm and emission wave-length at 332 nm. The method for tadalafil was found to be linear over the concentration range of 10-50 ng/mL with a correlation coefficient of 0.9991. Limit of detection and limit of quantification were found to be 0.235 ng/mL and 0.701 ng/mL, respectively. The method was validated and found to be suitable for the estimation of tadalafil from human plasma. Satisfactory recovery of tadalafil from the human plasma suggests no interference of any debris present into human plasma. This method can be used to deter-mine plasma tadalafil concentration in drug monitoring or pharmacokinetic investigation.

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UV-Visible Spectrophotometric Determination of Lambda-Cyhalothrin Insecticide in Vegetables, Soil and Water Samples

Journal of Ravishankar University (Part-B) ◽

10.52228/jrub.2018-31-1-1 ◽

2019 ◽

Vol 31 (1) ◽

pp. 1-9

Author(s):

Deepak Kumar Sahu ◽

Joyce Rai ◽

Chhaya Bhatt ◽

Manish K. Rai ◽

Jyoti Goswami ◽

...

Keyword(s):

Crop Production ◽

Limit Of Detection ◽

Low Cost ◽

Molar Absorptivity ◽

Agricultural Field ◽

Limit Of Quantification ◽

Yellow Colour ◽

Modern Age ◽

Lambda Cyhalothrin

In modern age pesticide is used widely in agriculture. Lambda-cyhalothrin (LCT) is one of the most used pesticides which are used as a insecticide to kill pest, tricks, flies etc in agricultural field and it is also used for crop production. We have developed new method to detect LCT insecticide in agriculture field and reduce its uses. In this method we found the maximum absorbance at 460 nm for yellow colour dye. We also calculated limit of detection and limit of quantification 0.001 mg kg-1 and 0.056 mg kg-1 respectively. Molar absorptivity and Sandell’s sensitivity was also calculated and obtained 1.782 ×107 mol-1 cm-1 and 9.996 ×10-6 µg cm-2 respectively. The obtained yellow colour dye obeyed Beer’s law limit range of 0.5 µg ml -1 to 16 µg ml-1 in 25 ml. This method is less time consuming, selective, simple, sensitive and low cost. Present method is successfully applied in various soil, water and vegetable samples.

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STABILITY INDICATING UPLC METHOD FOR ESTIMATION OF METFORMIN HYDROCHLORIDE AND NATEGLINIDE SIMULTANEOUSLY IN THE PRESENCE OF STRESS DEGRADATION PRODUCTS

INDIAN DRUGS ◽

10.53879/id.57.05.11332 ◽

2020 ◽

Vol 57 (05) ◽

pp. 56-64

Author(s):

Rani A Prameela ◽

S. Madhavi ◽

Rao B. Tirumaleswara ◽

Sudheer Reddy CH.

Keyword(s):

Limit Of Detection ◽

Degradation Products ◽

Potassium Dihydrogen Phosphate ◽

Metformin Hydrochloride ◽

Forced Degradation ◽

Dihydrogen Phosphate ◽

Limit Of Quantification ◽

Pharmaceutical Dosage Forms ◽

Column Temperature

A novel Ultra Performance Liquid Chromatography (UPLC) method was developed and validated for the simultaneous determination of antidiabetic drugs metformin hydrochloride and nateglinide. The method was developed using a Waters ACQUITY UPLC SB C18 (100 × 2.1 mm, 1.8 μm) column. The mobile phase consisting of 0.01 % potassium dihydrogen phosphate buffer (pH 5.8): acetonitrile (50: 50 V/V) was used throughout the analysis. The flow rate was 0.3 mL/min, the injection volume was 1.0 μL, column temperature was 30 0C, run time 3 min and detection was carried at 238 nm using a TUV detector. The retention times of metformin hydrochloride and nateglinide were found to be 1.28 1.71 min, respectively. Metformin hydrochloride and nateglinide were found to be linear over the concentration range of 125-750 and 15-90 μg/mL. The limit of detection and the limit of quantification for metformin hydrochloride were found to be 0.22 and 0.68 μg/mL, respectively, and, for nateglinide, 0.02 and 0.6 μg/mL, respectively. Developed method was validated as per ICH guidelines. The specificity of the method was confirmed by forced degradation study. The suggested method is suitable for determination of metformin hydrochloride and nateglinide in bulk and pharmaceutical dosage forms.

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