Efek kandungan serat beras analog terhadap ekspresi GLUT4 otot rangka tikus diabetes

Effect of analog rice’s fiber on skeletal muscles GLUT4 expression in diabetic rats Background: Disruption of glucose transportation in skeletal muscle through GLUT4 becomes a problem in diabetes. Analog rice that had been modified by adding dietary fiber could improve the expression of GLUT4.Objective: This study aims to know the effect of dietary fiber toward GLUT4 expression and to know the dietary fiber percentage in analog rice.Method: The research type is true experimental with post-test only group design. The samples consist of 24 male Wistar rats that are group into 4 groups (n=6 each group). Three groups were induced by giving a high-fat diet for 40 days and streptozotocin (STZ) 35 mg/kg BW was given at 33th day and one group was not induced. After the blood glucose level exceeded 135 mg/dl, the treatment was given. After 3 weeks, the rats were terminated and quadriceps femoris muscle tissue was taken for immunohistochemistry examination using rat GLUT4 polyclonal antibody. GLUT4 expression was quantified using an immunoreactive score (IRS-GLUT4). The data were analyzed using the Kruskal-Wallis test and Spearman test.Results: Statistical analyses showed that there were significant differences between groups with a moderate positive correlation (correlation coefficient=0,651; p=0,003).Conclusion: Dietary fiber in analog rice could improve skeletal muscle GLUT4 expression in Wistar rat diabetic model.

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Anti-Diabetic Effect of a Polysaccharide Fraction From Momordica Charantia: Modulation of Notch Signaling

10.21203/rs.3.rs-516808/v1 ◽

2021 ◽

Author(s):

Soraya Sajadimajd ◽

Bahareh Mohammadi ◽

Gholamreza Bahrami ◽

Seyed Hamid Madani ◽

Razieh Hatami ◽

...

Keyword(s):

Notch Signaling ◽

Normal Tissue ◽

Metabolic Diseases ◽

Diabetic Rats ◽

Mrna Levels ◽

Carbohydrate Structure ◽

Immunohistochemistry Staining ◽

Diabetic Model ◽

Male Wistar Rats ◽

The Impact

Abstract Given the impact of notch signaling in the modulation of metabolic diseases and normal tissue homeostasis, this study aimed to evaluate whether notch signaling has a role in anti-diabetic and islet regenerative effects of isolated polysaccharide from Memordica charantia in diabetic rats. The polysaccharide was isolated from Memordica charantia (MCP) and characterized using FTIR and LC-MS/MS. Diabetic model was established by intrapritoneal administration of STZ in male Wistar rats. The levels of Hes1, Notch 1, DLL4, Jagged1, Pdx1, CD34, CD31 and VEGF were analyzed by using immunohistochemistry and real-time PCR. Structural analyses have revealed the carbohydrate structure of fraction. Blood glucose was halted by treatment with fraction. MCP scaled up the mRNA levels of Ins1, jagged1, Pdx1 and Hes1 while scaled down the levels of Notch1, Dll4 and the ratio of Bax/Bcl2 in diabetic rats. Furthermore, the immunohistochemistry staining of hes1, cyclin d1 and VEGF proteins was increased in the pancreas of MCP-treated diabetic rats compared to the diabetic group. These findings provide insights into the anti-diabetic potential of MCP through modulation of islets regeneration and suggest that modulation of notch and angiogenesis pathways may play the pivotal role in the restoration of islets to relieve diabetes.

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Effect of iridoid glucoside on plasma lipid profile, tissue fatty acid changes, inflammatory cytokines, and GLUT4 expression in skeletal muscle of streptozotocin-induced diabetic rats

Molecular and Cellular Biochemistry ◽

10.1007/s11010-013-1656-0 ◽

2013 ◽

Vol 380 (1-2) ◽

pp. 43-55 ◽

Cited By ~ 8

Author(s):

Ramalingam Sundaram ◽

Palanivelu Shanthi ◽

Panchanatham Sachdanandam

Keyword(s):

Skeletal Muscle ◽

Fatty Acid ◽

Lipid Profile ◽

Inflammatory Cytokines ◽

Plasma Lipid ◽

Diabetic Rats ◽

Plasma Lipid Profile ◽

Iridoid Glucoside ◽

Tissue Fatty Acid ◽

Glut4 Expression

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Hubungan Dosis Tepung Gembili (Dioscorea esculenta) Dengan Tingkat Ekspresi Enzim Ampk-α2 pada Model Tikus Diabetes Melitus

Medical Laboratory Technology Journal ◽

10.31964/mltj.v1i1.4 ◽

2015 ◽

Vol 1 (1) ◽

pp. 7

Author(s):

Neni Oktiyani ◽

Sunarti Sunarti ◽

Prasetyastuti Prasetyastuti ◽

Jujuk Anton Cahyono

Keyword(s):

Skeletal Muscle ◽

Glucose Production ◽

Diabetic Rats ◽

Alternative Methods ◽

Glucose Levels ◽

Patients With Diabetes ◽

Post Test ◽

Quasi Experimental ◽

Yam Flour

Alternative methods of controlling glucose levels in patients with diabetes is by type of food, either by utilizing yam flour. At the flour contained inulin and resistant starch that can activate the enzyme AMPK-α2. Activation of these enzymes will stimulate glucose transport in skeletal muscle and liver, thus causing a decrease in glucose production. Varying doses of flour is expected to affect the expression of AMPK-α2. This study aims to dosage relationship yam flour (Dioscorea esculenta) with tigkat-α2 AMPK enzyme expression in the nucleus skeletal muscle and liver in mouse models of diabetes mellitus. The study was a quasi-experimental design with Post Test Only Group Design. Rats were divided into 5 (five) groups, healthy mice, the mice with type 2 diabetes, and type 2 groups of diabetic rats with dietary intake yam flour addition of 1.25 g (TG-1:25),), 2.5 g (TG -2.5), 5.0 g (TG-5.0). Yam flour is mixed into the rat diet feed with varying doses. The results showed no significant correlation between the dose of yam flour with AMPK-α2 expression levels in skeletal muscle nuclei (p = 0.312) and liver (p = 0.474) in a mouse model of DM. The need for other studies using other types of food as an alternative arrangement of food for patients with diabetes

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Bethanechol and N-acetylcysteine mimic feeding signals and reverse insulin resistance in fasted and sucrose-induced diabetic rats

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y11-001 ◽

2011 ◽

Vol 89 (2) ◽

pp. 135-142 ◽

Cited By ~ 10

Author(s):

W. Wayne Lautt ◽

Joshua Schafer ◽

M. Paula Macedo ◽

Dallas J. Legare

Keyword(s):

Insulin Resistance ◽

Skeletal Muscle ◽

Diabetic Rats ◽

Similar Degree ◽

Fasted State ◽

Diabetes Model ◽

Diabetic Model ◽

Hepatic Glutathione ◽

Necessary And Sufficient ◽

Fasted Rats

Meal-induced insulin sensitization (MIS) is explained by the HISS (hepatic insulin sensitizing substance) hypothesis. In the presence of two “feeding signals,” a pulse of insulin results in the release of HISS from the liver. HISS acts selectively on skeletal muscle and doubles the response to insulin. HISS is not released in the fasted state or in the sucrose-supplemented diabetes model. We tested the hypothesis that provision of both feeding signals allows insulin to cause HISS release in both the normal fasted and the diabetic model. The dynamic response to insulin (50 mU/kg over 5 min) was quantified using the rapid insulin sensitivity test (RIST). Gastric injection of a liquid test meal or i.v. administration of N-acetylcysteine in 24 h fasted rats raised hepatic glutathione to a similar degree (by 46%–47%). Hepatic denervation in fed rats eliminated the parasympathetic signal and eliminated MIS, and bethanechol completely restored MIS. Both compounds administered together allowed insulin to stimulate HISS release in 24 h fasted rats and in a diabetic model (9-week, 35% liquid sucrose supplement). Neither was effective alone. Both “feeding signals” are necessary and sufficient for insulin to stimulate HISS release.

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The Effect of Simvastatin on Glucose Homeostasis in Streptozotocin Induced Type 2 Diabetic Rats

Journal of Diabetes Research ◽

10.1155/2013/274986 ◽

2013 ◽

Vol 2013 ◽

pp. 1-5 ◽

Cited By ~ 9

Author(s):

Lulu Wang ◽

Guanglan Duan ◽

Yong Lu ◽

Shuguang Pang ◽

Xianping Huang ◽

...

Keyword(s):

Normal Control ◽

Glucose Homeostasis ◽

Density Lipoprotein ◽

Diabetic Rats ◽

Standard Diet ◽

Simvastatin Treatment ◽

Diabetic Model ◽

Male Wistar Rats ◽

Type 2 Diabetic

Objective. To investigate the effect of simvastatin on glucose homeostasis in streptozotocin induced type 2 diabetic rats.Methods. Forty male Wistar rats were randomly divided into four groups. Normal control rats were fed with standard diet, others were fed with high-fat diet. Diabetic rats were induced by a single intraperitoneal injection of STZ. The simvastatin intervention rats were fed with simvastatin during the experiment process, and the simvastatin treatment rats were fed with simvastatin after diabetes rats were induced. We measured body weight, fasting plasma glucose, cholesterol, high-density lipoprotein cholesterol, and triglyceride after an overnight fast.Results. The FPG was higher in diabetic rats when compared to normal control ones; the simvastatin intervention rats had a higher FPG compared to the diabetic rats and were more easily be induced to diabetes at the end of 4 weeks, FPG level of simvastatin treatment rats was increased compared with diabetic model rats after 12 weeks.Conclusion. These data indicate that simvastatin intervention rats may cause hyperglycemia by impairing the function of isletβcells and have an adverse effect on glucose homeostasis, especially on FPG level.

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The effect of tempeh intake on spatial memory in prediabetic rats

Nutrition & Food Science ◽

10.1108/nfs-03-2018-0071 ◽

2019 ◽

Vol 49 (4) ◽

pp. 592-599 ◽

Cited By ~ 1

Author(s):

Annisa Ayuningtyas ◽

Etisa Adi Murbawani ◽

Nuryanto Nuryanto

Keyword(s):

Body Weight ◽

Spatial Memory ◽

Travel Time ◽

Wistar Rat ◽

Control Group ◽

Control Group Design ◽

Content Type ◽

Post Test ◽

Male Wistar Rats ◽

The Central Nervous System

Purpose Prediabetes can affect the central nervous system and lead to changes including impaired cognition and spatial memory. Isoflavones, especially genistein and daidzein, can increase spatial memory. Tempeh is a soy product with high levels of genistein and daidzein. The purpose of this paper is to determine the effect of tempeh on spatial memory of prediabetic Wistar rat. Design/methodology/approach A pre-and post-test randomised control group design was used. In total, 15 male Wistar rats were divided into three equal groups, Treatment 1 (T1), Treatment 2 (T2) and Treatment 3 (T3). All rats were injected with alloxan (120 mg/Kg body weight) to induce prediabetes. T1 was maintained on a diet of standard food for 14 days. T2 and T3 were given 9 g/200 g body weight and 18 g/200 g body weight of tempeh, respectively, for 14 days. The Morris water maze (MWM) test was used to test spatial memory. Findings While there were no statistically significant differences between the treatment groups, there was a difference with the tempeh-treated rats improving their spatial memory following tempeh ingestion with a decrease in the travel time to negotiate the MWM [T1(−8.36), T2 (−5.48) and T3 (3.66)]. Differences of travel time of the three groups was not significant (p = 0.677). Originality/value The result of the study indicates that tempeh may act as a functional food in reducing cognitive impairment associated with prediabetes and diabetes. However, as the result is not statistically significant, further research using animal models and human studies is warranted.

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A Novel Role of Globular Adiponectin in Treatment with HFD/STZ Induced T2DM Combined with NAFLD Rats

The Scientific World JOURNAL ◽

10.1155/2014/230835 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 4

Author(s):

Hong Ma ◽

Guo-Ping You ◽

Xuan-Pu Zhang ◽

Xiang-Jiu Yang ◽

Hua-Dong Lu ◽

...

Keyword(s):

Skeletal Muscle ◽

Insulin Secretion ◽

Hepatic Steatosis ◽

Treated Group ◽

Diabetic Rats ◽

Normal Diet ◽

Adiponectin Receptors ◽

Male Wistar Rats ◽

Globular Adiponectin

Aims. To evaluate the effects of globular adiponectin (gAd) on treatment of type 2 diabetic rats combined with NAFLD.Materials and Methods. Twenty-one male wistar rats were fed with normal diet (7 rats) or high fat diet (HFD) (14 rats) for 4 weeks, and then HFD-fed rats were injected with streptozotocin (STZ) to induce type 2 diabetes mellitus (T2DM). Half of T2DM rats were randomly injected with gAd intraperitoneally for 7 days. The expressions of adiponectin receptors (adipoR1/R2) in liver and skeletal muscle tissues were detected through western blotting or RT-qPCR, respectively.Results. Globular adiponectin alleviated the hepatic steatosis and increased insulin secretion. In liver, both the protein and mRNA expressions of adipoR2 in T2DM group decreased (P<0.05, resp.) in contrast to NC group and increased (P<0.05andP<0.001, resp.) after gAd treatment. But the protein and mRNA expressions of adipoR1 increased (P<0.05, resp.) in T2DM group and no change was found in the gAd-treated group. In skeletal muscle, the protein and mRNA expressions of adipoR1 and adipoR2 were upregulated in T2DM group and were downregulated after gAd treatment.Conclusions. Globular adiponectin could ameliorate the hepatic steatosis and vary the expressions of adiponectin receptors in liver and skeletal muscle by stimulating insulin secretion.

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Effects of Exercises on IL-6 and GLUT4 Expression in Diabetic Rats

Research in Molecular Medicine ◽

10.18502/rmm.v6i2.4539 ◽

2019 ◽

Author(s):

Ali Dehghan ◽

Parvin Farzanegi ◽

Hajar Abbaszadeh

Keyword(s):

Skeletal Muscle ◽

Blood Glucose ◽

Patient Group ◽

Molecular Mechanisms ◽

Insulin Level ◽

Exercise Group ◽

Diabetic Rats ◽

Control Group ◽

Insulin Levels ◽

Glut4 Expression

Background: Diabetes mellitus is one of the most common metabolic disorders worldwide. Empirical evidence has shown exercise to be of value in ameliorating symptoms of diabetes, but the underlying molecular mechanisms involved have not been well-studied. Aim: To evaluate the effects of continuous and interval exercises on blood glucose and insulin levels and on IL-6 and GLUT4 expression in diabetic rats. Materials and Methods: Old Wistar rats with streptozotocin-induced diabetes were randomly divided into three groups; namely, the patient (non-exercise control) group, the interval exercise group, and the continuous exercise group. The blood insulin level was measured using a specific ELISA kit, whereas the blood glucose level was determined with an AutoAnalyzer apparatus. Expression of the IL-6 and GLUT4 genes in skeletal muscle was determined using real-time PCR. All data were analyzed using SPSS software. Results: The expression of IL-6 and GLUT4 was significantly decreased in skeletal muscle of the diabetic rats (p < 0.001). Continuous (9.97-fold; p = 0.000) and interval (7.11-fold; p = 0.000) exercises significantly increased the expression of IL-6 relative to that in the animals of the patient group. Furthermore, continuous (9.36-fold; p = 0.000) and interval (7.65-fold; p = 0.001) exercises also significantly increased the expression of GLUT4 relative to that of the patient group. Both types of exercise training were associated with a significant decrease in the blood glucose and insulin levels compared with the levels in the patient group (p < 0.0001). Conclusions: Diabetes is significantly associated with IL-6 and GLUT4 downregulation, but this can be reversed through continuous and interval exercises, which also help to lower the blood glucose and insulin levels significantly.

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