scholarly journals The Discovery of Vibrio harveyi on Litopenaeus vannamei Infected White Feces Disease in Situbondo, East Java

2020 ◽  
Vol 22 (1) ◽  
pp. 9
Author(s):  
Sumini Sumini ◽  
Rahayu Kusdarwati
Keyword(s):  
Water Quality ◽  
Vibrio Harveyi ◽  
Total Organic Matter ◽  
Bacterial Isolates ◽  
Abnormal Result ◽  
Biochemical Tests ◽  
Specific Primers ◽  
Poor Water Quality ◽  
Brackishwater Pond ◽  
Test Result

This research was conducted to discover Vibrio harveyi infected vannamei shrimp with White Feces Disease (WFD) in Situbondo, East Java Province. This research was conducted in November 2017 until September  2018. All Vibrio isolates from shrimp infected WFD were identified with biochemical tests, Analytical Profile Index/ API 20NE (BioMeriuex), and PCR with specific primers for V. harveyi. Additional parameters were the water quality, plankton brackishwater pond abundance, and antibiotic resistance test. Result showed that from 17 bacterial isolates identified, 10 isolates were V. harveyi (58.82%), three isolates were V. alginolyticus (17.65%), one V. fluvialis isolate (5.88%), one V. parahaemolyticus isolate (5.88%), and two non Vibrio isolates which were identified as Shewanella putrefaciens (11.76%). All isolates of V. harveyi also showed resistance activity on more than one antibiotic. Poor water quality had been identified as abnormal result of pH, alkalinity, salinity, ammonia levels and total organic matter level. Plankton abundance observation showed that Chloropyceae, Diatom, and Dinoflagellata dominated all sampled brackishwater ponds. This research concluded that V. harveyi were discovered on vannamei shrimp with poor water quality and plankton abundance in the pond samples.

scholarly journals Detection of Polymorphism in the Gene blaKPC2 of Local Klebsiella pneumoniae Isolated from Iraqi Patients

2017 ◽  
Vol 11 (1) ◽  
pp. 26-33
Author(s):  
Saadi Abd-Alkareem Jasim ◽  
Mohammed Maaroof ◽  
Najwa Ahmed
Keyword(s):  
Environmental Management ◽  
Klebsiella Pneumoniae ◽  
Dna Sequences ◽  
Microscopic Examination ◽  
Gene Bank ◽  
Bacterial Isolates ◽  
Wild Type ◽  
Biochemical Tests ◽  
Specific Primers ◽  
Severe Infections

Carbapenemases are clinically important because they destroy and may confer a resistance to carbapenems, severe infections caused by carbapenemase producers is associated with increased mortality. To achieve this goal, 180 samples were collected from different clinical sources included 92 urine, 33 smears of wounds, 13 smears of burns and 42 sputum. The samples were taken from patients attended Al-Yarmouk Teaching Hospital and Ibin Baladi Hospital in Baghdad Governorate. Diagnosis of bacterial isolates was done depending upon the microscopic examination, the cultured characteristics and biochemical tests. DNA extracted from 84 samples. Accordingly, detection of blaKPC2 gene was conducted by using specific primers for amplification of blaKPC2 gene. Moreover, the sequencing of 910 bp for blaKPC2 gene was performed by the biotechnology lab. at the National Instrumentation Center for Environmental Management (NICEM). Such test has been implanted by using 3730XL as a DNA sequences. The obtained results were analyzed by blast at the National Center Biotechnology Information (NCBI) and detect polymorphism in blaKPC2 based on the Bio Edit. Consequently, 94 variations between 47 transversions, 43 transitions and 4 deletions nucleotide were noticed. In a sense the test showed 79% under sequence ID gb|CP009872.1| from 3037673 -3038166 number of nucleotide from K. pneumoniae subsp. pneumoniae strain KPNIH30 of Gene Bank, score (329) and expect 5e-86 with the wild type of blaKPC2 gene from Gene Bank. Finally, the results illustrated polymorphism between local strains of

Identification Pseudomonas aeruginosa by OprD Gene for Differentiation from Other Pseudomonas Species that Isolated from Clinical Samples

2019 ◽  
Vol 9 (01) ◽  
pp. 46-50
Author(s):  
Ashwak B Al-Hashimy ◽  
Huda S Alagely ◽  
Akeel K Albuaji ◽  
Khalid R Majeed
Keyword(s):  
Pseudomonas Aeruginosa ◽  
General Hospital ◽  
Culture Media ◽  
Final Diagnosis ◽  
Clinical Samples ◽  
Bacterial Isolates ◽  
Molecular Method ◽  
Biochemical Tests ◽  
Pseudomonas Species ◽  
Specific Sequences

The present study included the collection of 100 samples from various clinical sources for investigating the presence of P. aeruginosa in those sources, the samples have been collected from some hospitals in Baghdad and Hillah city (Al-qassim General Hospital, ,Al-hillah teaching hospital,and Al-hashimya General hospital ) which included wounds, burns, ear and sputum infections. The study was carried out through October 2017 till the end of March 2018. The samples were identified based on the morphological and microscopically characteristics of the colonies when they were culturing or number of culture media as well as biochemical tests, molecular identification were also used as a final diagnostic test for isolates that were positive as they belong to P.aeruginosa bacteria during previous tests based on the OprD gene which has specific sequences for P.aeruginosa bacteria as a detection gene and also consider as virulence factor so it have a synonyms mechanism to antibiotic resistance . The results of the final diagnosis showed that 38 isolates belong to target bacteria were distributed as 18 of burns, 11 isolates of wounds, 6 isolates of ear infection and 3 isolates of sputum, The examination of the sensitivity of all bacterial isolates was done for elected 38 isolation towards the 9 antibiotic by a Bauer - Kirby and the isolates were resistant for a number of antibiotics used such as Ciprofloxacin 65.7%, Norflaxacin 71%, Imipenem 63.1% Meropenem 68.4%, Gentamicin 65.7%, Amikacin 26.3%, Cefepime 68.4%, Ceftazidime 65.7% and Piperacillin 57.8%.Molecular method , All isolates (38) of P. aeruginosa positive for the diagnostic special gene (OprD) genes (100%).

Phylogeny Characterization of Seb Gene Encoding Enterotoxins in Staphylococcus aureus Isolated from Raw Milk and Cheese

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Fatima N. Aziz ◽  
Laith Abdul Hassan Mohammed-Jawad
Keyword(s):  
Staphylococcus Aureus ◽  
Food Poisoning ◽  
Raw Milk ◽  
Staphylococcal Enterotoxin B ◽  
Human Pathogen ◽  
Conventional Pcr ◽  
Biochemical Tests ◽  
Specific Primers ◽  
Gene Encoding

Food poisoning due to the bacteria is a big global problem in economically and human's health. This problem refers to an illness which is due to infection or the toxin exists in nature and the food that use. Milk is considered a nutritious food because it contains proteins and vitamins. The aim of this study is to detect and phylogeny characterization of staphylococcal enterotoxin B gene (Seb). A total of 200 milk and cheese samples were screened. One hundred ten isolates of Staphylococcus aureus pre-confirmed using selective and differential media with biochemical tests. Genomic DNA was extracted from the isolates and the SEB gene detects using conventional PCR with specific primers. Three staphylococcus aureus isolates were found to be positive for Seb gene using PCR and confirmed by sequencing. Sequence homology showed variety range of identity starting from (100% to 38%). Phylogenetic tree analyses show that samples (6 and 5) are correlated with S. epidermidis. This study discovered that isolates (A6-RLQ and A5-RLQ) are significantly clustered in a group with non- human pathogen Staphylococcus agnetis.

Problems and practical experiences during refilling of the Kerspe-Talsperre under unfavourable climatic conditions

1998 ◽  
Vol 37 (2) ◽  
pp. 145-152 ◽  
Author(s):  
A. E. Nienhüser ◽  
P. Braches
Keyword(s):  
Water Quality ◽  
Early Summer ◽  
Climatic Conditions ◽  
Oxygen Deficit ◽  
Light Limitation ◽  
Raw Water ◽  
Artificial Mixing ◽  
Poor Water Quality ◽  
High Concentrations ◽  
Practical Experiences

Refilling of the Kerspe-Talsperre reservoir after restoration of the dam caused severe problems in water quality and supply due to a long-lasting ice-cover period and minimal precipitation. Unusually short spring overturn, high algal mass in spring and certainly the overflooding of the sediment, which had not been removed, caused a tremendous oxygen deficit in early summer. The whole hypolimnion turned anoxic and even in the metalimnion oxygen declined to a minimum. Manganese and iron reached high concentrations. Despite the poor water quality, raw water was urgently needed for the water supply and was pumped from the epilimnion during the clear water phase and in July when algal biomass was low. In the middle of August holomixis was induced artificially in order to improve water quality. The decline in algal production during and after artificial mixing was probably caused by light limitation of the algae.

scholarly journals Classification Maps for TDS Concentrations in the GIS Along Euphrates River, Iraq

2021 ◽  
Vol 232 (8) ◽  
Author(s):  
Ali Chabuk ◽  
Zahraa Ali Hammood ◽  
Nadhir Al-Ansari ◽  
Salwan Ali Abed ◽  
Jan Laue
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
World Health Organization ◽  
World Health ◽  
Euphrates River ◽  
Poor Water Quality ◽  
Quality Category ◽  
Health Organization ◽  
Dissolved Salts

AbstractIraq currently undergoing the problem of water shortage, although Iraq has two Rivers (Euphrates and Tigris) pass throughout most of its areas, and they have represented a major source of water supply. In the current research, to evaluate the quality of the Euphrates river in Iraq based on the values of total dissolved salts (TDS), the TDS concentrations were collected from sixteen sections along the river in the three succeeding years (2011, 2012, and 2013). The evaluation of the river was done depending on the classification of (W.H.O. (World Health Organization). (2003). Total Dissolved Salts in Drinking-water: Background document for development of W.H.O. Guidelines for Drinking-water Quality. World Health Organization, 20 Avenue Appia, 1211 Geneva 27, Switzerland). of rivers for drinking uses. Inverse Distance Weighting Technique (IDWT) as a tool in the GIS was employed to establish the maps of the river that using interpolation/prediction for the TDS concentrations to each selected year and the average values of TDS for these 3 years. Based on the five categories of rivers’ classification of the TDS concentrations according to the (W.H.O. (World Health Organization). (2003). Total Dissolved Salts in Drinking-water: Background document for development of W.H.O. Guidelines for Drinking-water Quality. World Health Organization, 20 Avenue Appia, 1211 Geneva 27, Switzerland), the Euphrates river was classified, and the maps of classification for the years 2011, 2012 and 2013 and the average values for 3 years were created. The average values for 3 years of TDS along the Euphrates river indicated that the sections from SC-1 to SC-4 as moderate-water-quality-Category-3, the sections from SC-5 to SC-10 as poor-water-quality-Category-4, while the sections between SC-11 to SC-16 as very poor-water-quality-Category-5. The interpolation maps showed that the Euphrates river in Iraq was ranged from moderate water quality (Category-3) to very poor water quality (Category-5).

scholarly journals Water quality and antifungal susceptibility of opportunistic yeast pathogens from rivers

2016 ◽  
Vol 75 (6) ◽  
pp. 1319-1331 ◽  
Author(s):  
M. E. Monapathi ◽  
C. C. Bezuidenhout ◽  
O. H. J. Rhode
Keyword(s):  
Water Quality ◽  
Antifungal Susceptibility ◽  
Principal Component ◽  
Water Sources ◽  
Health Concern ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Livestock Farming ◽  
Physico Chemical ◽  
26S Rrna

Yeasts from water sources have been associated with diseases ranging from superficial mucosal infections to life threatening diseases. The aim of this study was to determine the water quality as well as diversity and antifungal susceptibility of yeasts from two rivers. Yeast levels and physico-chemical parameter data were analyzed by principal component analysis to determine correlations between physico-chemical data and yeast levels. Yeast morphotypes were identified by biochemical tests and 26S rRNA gene sequencing. Disk diffusion antifungal susceptibility tests were conducted. Physico-chemical parameters of the water were within target water quality range (TWQR) for livestock farming. For irrigational use, total dissolved solids and nitrates were not within the TWQR. Yeast levels ranged between 27 ± 10 and 2,573 ± 306 cfu/L. Only non-pigmented, ascomycetous yeasts were isolated. Saccharomyces cerevisiae and Candida glabrata were most frequently isolated. Several other opportunistic pathogens were also isolated. A large number of isolates were resistant to azoles, especially fluconazole, but also to other antifungal classes. Candida species were resistant to almost all the antifungal classes. These water sources are used for recreation and religious as well as for watering livestock and irrigation. Of particular concern is the direct contact of individuals with opportunistic yeast, especially the immune-compromised. Resistance of these yeast species to antifungal agents is a further health concern.

Water Quality Violations and Avoidance Behavior: Evidence from Bottled Water Consumption

2011 ◽  
Vol 101 (3) ◽  
pp. 448-453 ◽  
Author(s):  
Joshua Graff Zivin ◽  
Matthew Neidell ◽  
Wolfram Schlenker
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Willingness To Pay ◽  
Water Consumption ◽  
Avoidance Behavior ◽  
Bottled Water ◽  
Poor Water Quality ◽  
Using Data ◽  
The Impact

We examine the impact of poor water quality on avoidance behavior by estimating the change in bottled water purchases in response to drinking water violations. Using data from a national grocery chain matched with water quality violations, we find an increase in bottled water sales of 22 percent from violations due to microorganisms and 17 percent from violations due to elements and chemicals. Back-of-the envelope calculations yield costs of avoidance behavior at roughly $60 million for all nationwide violations in 2005, which likely reflects a significant understatement of the total willingness to pay to eliminate violations.

scholarly journals A novel emotional and cognitive approach to welfare phenotyping in rainbow trout exposed to poor water quality

2019 ◽  
Vol 210 ◽  
pp. 103-112 ◽  
Author(s):  
V. Colson ◽  
A. Mure ◽  
C. Valotaire ◽  
J.M. Le Calvez ◽  
L. Goardon ◽  
...  
Keyword(s):  
Water Quality ◽  
Rainbow Trout ◽  
Cognitive Approach ◽  
Poor Water Quality

scholarly journals EVALUATION GROUNDWATER QUALITY BY USING GIS AND WATER QUALITY INDEX TECHNIQUES FOR WELLS IN BARDARASH AREA, NORTHERN IRAQ

2020 ◽  
Vol 53 (2C) ◽  
pp. 87-104
Author(s):  
Kaiwan Fatah
Keyword(s):  
Water Quality ◽  
Groundwater Quality ◽  
Water Quality Index ◽  
Quality Index ◽  
Total Alkalinity ◽  
Total Hardness ◽  
Chemical Parameters ◽  
Alternative Source ◽  
Distribution Maps ◽  
Poor Water Quality

Studying groundwater quality in arid and semi-arid regions is essential significant because it is used as a foremost alternative source for various purposes (human and animal consumption, economic, agriculture and irrigation). Geographic Information System and Water Quality Index techniques were utilized for visualizing and evaluating the variations of groundwater quality in the studied area. Total twelve wells were sampled and twelve groundwater quality (chemical) parameters; pH, Total Alkalinity, Total Hardness (TH), Total Dissolved Solid (TDS), Electrical Conductivity (Ec), Potassium (K), Nitrate (NO3), Sulfate (SO4), Chloride (Cl), Calcium (Ca), Magnesium (Mg) and Sodium (Na) were analyzed in the laboratory. Inverse Distance Weighted technique was used as a useful tool to create and anticipate spatial variation maps of the chemical parameters. Predicting or anticipating other areas not measured, identifying them and making use of them in the future without examining samples. The results of this research showed that 8.3% of the studied wells have excellent groundwater quality, and almost sampling wells about 75% found in good groundwater quality, while findings of groundwater quality of 16.7% studied wells belong to poor water quality due to standards of Water Quality Index. Moreover, spatial analysis in term of groundwater quality map showed that Excellent groundwater quality was detected in well 3, very good groundwater potential was noticed in six studied wells (wells 2, 6, 8, 10, 11 and 12), and other sampling wells (wells 4 and 7) were observed as good groundwater quality, while poor water quality was observed in wells (well 1 and 5). Hence, spatial distribution maps showed that the almost groundwater quality in the area about 1046.82 km² (99.04%) are suitable for drinking purpose, whereas proximate 10.18 km² (0.96%) are observed as poor water quality and inappropriate for consumptions especially in the southern part of the area.

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