scholarly journals EVALUATION OF IN VITRO INHIBITION OF PROTON􀇧PUMP BY NANOSILVER PARTICLES SYNTHESIZED USING SEEDS OF ANETHUM GRAVEOLENS

2017 ◽  
Vol 10 (6) ◽  
pp. 363
Author(s):  
Menaga S ◽  
Kripa Kg ◽  
Sangeetha R
Keyword(s):  
Green Synthesis ◽  
Proton Pump ◽  
Surface Composition ◽  
Green Technology ◽  
X Ray Diffraction ◽  
Anethum Graveolens ◽  
Standard Drug ◽  
Synthesis Of Nanoparticles ◽  
Nanosilver Particles

Objective: The green synthesis of nanoparticles is gaining popularity owing to its several advantages over the chemical methods. This study reports the biosynthesis of nanosilver mediated by the extract of the seeds of Anethum graveolens.Methods: Green synthesis of silver nanoparticles (AgNPs) was carried out using the aqueous extract of the seeds of A. graveolens (AESE). Microwave irradiation was done to hasten the process. The AgNPs synthesized were physically characterized using ultraviolet-visible spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy. The potential of the nanosilver particles in inhibiting H+ K+ adenosine triphosphatase (proton pump) was evaluated.Results: The nanosilver particles were initially subjected to characterization studies to ascertain their shape, size and surface composition. The particles were found to be crystalline, spherical of size 30-35 nm. The nanosilver particles inhibited the activity of proton pump by 67 % and thus were as potential as the standard drug, omeprazole.Conclusion: The seeds have several pharmacological benefits including its gastroprotective potential and hence the nanosilver particles synthesized using AESE was assessed for its gastroprotective potential. The results of this study prove that the nanosilver synthesized by green technology can inhibit proton-pump and thus act as promising gastroprotective agents.

scholarly journals Design, Synthesis and Pharmacological Evaluation of Novel Imidazopyridine Analogues as Proton Pump Antagonist

2020 ◽  
Vol 32 (4) ◽  
pp. 776-782
Author(s):  
Ravindra S. Sonawane ◽  
Kiran D. Patil ◽  
Avinash V. Patil
Keyword(s):  
Spectral Analysis ◽  
Proton Pump Inhibitors ◽  
Proton Pump ◽  
Inhibitory Activity ◽  
Positive Control ◽  
Docking Studies ◽  
Design Synthesis ◽  
Standard Drug ◽  
Imidazopyridine Derivatives

A series of novel imidazopyridine derivatives as proton pump inhibitors was designed with compounds of CID data base and explored considering AZD0865 as standard. Many compounds were identified and docked in proton pump ATPase pocket (PDB ID: 4ux2). Molecular docking studies revealed that many compounds showed good proton pump ATPase inhibitory activity. The docking poses revealed the interaction of ligands with amino acid. The standard drug AZD0865 had docking score of -7.112302 and displayed interactions with Asn138 and Asp137. A series of novel imidazopyridine derivatives as proton pump inhibitors were docked, synthesized and characterized by IR, NMR, CHN and MS spectral analysis. The target imidazopyridines were prepared from substituted 2-aminonicotinic acid and 2-bromo-1-substituted ethanone. in vitro Studies explained that few compounds exhibited moderate to good proton pump ATPase inhibitory activity in comparison with the reference drugs i.e. AZD0865. Compounds 11 and 12 shown higher activities with the IC50 4.3. Compounds 1, 4, 6, 7, 8, 10 and 13 showed weak anti-ulcer activity with its IC50 5.2, 5.8, 5.5, 5.1, 4.9, 4.6 and 5.9 and positive control AZD0865 shown IC50 2.0.

scholarly journals Green synthesis of Ag/TiO2 Nanocomposite Assisted by Gambier Leaf (Uncaria gambir Roxb) Extract

2019 ◽  
Vol 22 (6) ◽  
pp. 250-255 ◽  
Author(s):  
Sry Wahyuni ◽  
Syukri Syukri ◽  
Syukri Arief
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Diffuse Reflectance Spectroscopy ◽  
Irradiation Time ◽  
Rutile Phase ◽  
X Ray Diffraction ◽  
Silver Surface ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Vis Spectroscopy

Studies of green synthesis of nanoparticles mediated by plants extract is extensively explored and studied in recent time due to eco-friendliness, cost-effectiveness, and use a minimum amount of toxic chemicals in the process of inorganic material synthesis. In this study, the immobilization of silver nanoparticles on the surface of titanium dioxide (TiO2) was carried out using Uncaria gambier Roxb. leaf extract as a silver ion (Ag+) reducing agent. The synthesized Ag/TiO2 nanocomposite was characterized by UV-Vis spectroscopy, X-ray diffraction (XRD), and Diffuse Reflectance Spectroscopy (DRS). The formation of silver nanoparticles was confirmed through UV-Vis spectroscopic analysis, which showed a silver surface plasmon resonance (SPR) band at 426 nm. The X-ray diffraction pattern shows that Ag can inhibit the transition of the anatase into rutile phase. The presence of Ag particles in TiO2 can increase the absorption ability from an initial wavelength of 407 nm to 438 nm. Based on the results of Rhodamin B degradation, it can be seen that Ag/TiO2 has a higher photocatalytic activity than bare TiO2 with 99% percent degradation at 120 minutes of irradiation time.

scholarly journals In Vitro Studies of the Antimicrobial and Free-Radical Scavenging Potentials of Silver Nanoparticles Biosynthesized From the Extract of Desmostachya bipinnata

2018 ◽  
Vol 13 ◽  
pp. 117739011878287 ◽  
Author(s):  
Sitaramanjaneya Reddy Guntur ◽  
NS Sampath Kumar ◽  
Manasa M Hegde ◽  
Vijaya R Dirisala
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Radical Scavenging ◽  
X Ray Diffraction ◽  
X Ray ◽  
Gram Positive Bacteria ◽  
Antimicrobial Studies ◽  
Hepg2 Cell Lines ◽  
Hydrazyl Radical

The aim of this study was to perform green synthesis of silver nanoparticles (AgNPs) from the leaf extract of Desmostachya bipinnata (Dharba), a medicinally important herb which is widely used across India. Synthesized AgNPs were analyzed by UV-Visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDAX). The results have confirmed that green synthesis of AgNPs leads to the fabrication of sphere-shaped particles with a diameter of 53 nm. Furthermore, these AgNPs were subjected to antioxidant and antimicrobial studies against gram-negative and gram-positive bacteria, where AgNPs at a concentration of 20 mg/mL showed highest zone of inhibition. Synthesized AgNPs were evaluated for their antioxidant activity by 1, 1-diphenyl-2-picryl hydrazyl radical (DPPH), H2O2, and superoxide inhibiting assays; increasing concentration has showed increase in scavenging ability. Cell toxicity was assessed on HepG2 cell lines, and synthesized nanoparticles at a concentration of 128 μg/mL produced significant reduction in viability of Hep cells ( P < .05). The availability of Dharba throughout the year and the eco-friendly approach in the synthesis of AgNPs coupled with bioactivity has demonstrated its potential as a novel biomaterial which can be used for various biomedical applications.

scholarly journals Green Synthesis, Characterization and Antibacterial Study of Ag-Au Bimetallic Nanocomposite using Tea Powder Extract

2020 ◽  
Vol 11 (1) ◽  
pp. 8087-8095
Keyword(s):  
Green Synthesis ◽  
Biological Behavior ◽  
X Ray Diffraction ◽  
Antibacterial Study ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Transmission Electron ◽  
Silver Sample ◽  
Bimetallic Nanocomposite ◽  
Tea Extract

Synthesis of metal nanoparticles using plant extracts is attracted much to the recent researchers due to its simplicity, which integrates chemical technology. Special attention is given to the green synthesis of nanoparticles by easily available plants with an eco-friendly system compared to other conventional methods. Silver-Gold nanocomposite (Ag-Au NCp’s) is synthesized by bi reduction of silver nitrate and gold chloride. These metal salts are simultaneously reduced by tea extract to form respective silver and gold nanocomposite. The structure and morphology of as-prepared Ag-Au NCp’s sample were characterized by employing powder X-ray diffraction (XRD) tool and by Scanning Electron Micrograph (SEM) tool, respectively. Fourier Transform Infrared (FTIR) spectral study was undertaken to know the bonding in the prepared silver sample. Morphology and particle size of the above composite was studied by the Transmission electron microscope (TEM) tool. Energy-dispersive X-ray analysis (EDX) study was undertaken to know the formation of Ag-Au NCp’s. Antibacterial activity study is undertaken to know its biological behavior.

scholarly journals Antioxidants: Classification, Natural Sources, Activity/Capacity Measurements, and Usefulness for the Synthesis of Nanoparticles

Materials ◽  
2021 ◽  
Vol 14 (15) ◽  
pp. 4135
Author(s):  
Jolanta Flieger ◽  
Wojciech Flieger ◽  
Jacek Baj ◽  
Ryszard Maciejewski
Keyword(s):  
Antioxidant Activity ◽  
Green Synthesis ◽  
Antioxidant Capacity ◽  
Metallic Nanoparticles ◽  
Food Additives ◽  
Free Radical Damage ◽  
Synthesis Of Nanoparticles ◽  
Natural Extracts ◽  
Reducing Substances

Natural extracts are the source of many antioxidant substances. They have proven useful not only as supplements preventing diseases caused by oxidative stress and food additives preventing oxidation but also as system components for the production of metallic nanoparticles by the so-called green synthesis. This is important given the drastically increased demand for nanomaterials in biomedical fields. The source of ecological technology for producing nanoparticles can be plants or microorganisms (yeast, algae, cyanobacteria, fungi, and bacteria). This review presents recently published research on the green synthesis of nanoparticles. The conditions of biosynthesis and possible mechanisms of nanoparticle formation with the participation of bacteria are presented. The potential of natural extracts for biogenic synthesis depends on the content of reducing substances. The assessment of the antioxidant activity of extracts as multicomponent mixtures is still a challenge for analytical chemistry. There is still no universal test for measuring total antioxidant capacity (TAC). There are many in vitro chemical tests that quantify the antioxidant scavenging activity of free radicals and their ability to chelate metals and that reduce free radical damage. This paper presents the classification of antioxidants and non-enzymatic methods of testing antioxidant capacity in vitro, with particular emphasis on methods based on nanoparticles. Examples of recent studies on the antioxidant activity of natural extracts obtained from different species such as plants, fungi, bacteria, algae, lichens, actinomycetes were collected, giving evaluation methods, reference antioxidants, and details on the preparation of extracts.

scholarly journals Fabrication and Characterization of Copper Nanoparticles by Green Synthesis Approach Using Plectranthus Amboinicus Leaves Extract

2020 ◽  
Author(s):  
Srividya Parthasarathy ◽  
Sanjana Jayacumar ◽  
Sudestna Chakraborty ◽  
Prathyusha Soundararajan ◽  
Darshani Joshi ◽  
...  
Keyword(s):  
Zeta Potential ◽  
Green Synthesis ◽  
Copper Nanoparticles ◽  
Leaf Extract ◽  
Cost Effective ◽  
Xrd Analysis ◽  
X Ray Diffraction ◽  
Copper Sulphate Solution ◽  
Synthesis Of Nanoparticles ◽  
Plectranthus Amboinicus

Abstract The field of nanotechnology is gaining interest among the researchers towards the eco-friendly way of synthesis of nanoparticles. In this project, green synthesis technique was employed to induce the synthesis of copper nanoparticles using Plectranthus amboinicus, i.e. Mexican mint, identified as Coleus amboinicus leaf extract. We report an eco-friendly synthesis of copper nanoparticle using Plectranthus amboinicus leaf extract, which is a simple and an ostentatiously rapid method which produces stable nanoparticles. The copper sulphate solution was naturally employed as a precursor for synthesizing the copper nanoparticles. The extract of the plant Plectranthus amboinicus was found to showcase excellent reducing and stabilizing properties. By using Ultraviolet-Visible spectroscopy, Zeta Potential, and X-Ray Diffraction (XRD) studies, it was confirmed that copper nanoparticles have been synthesized. The UV-Spectrometer analysis shows the characteristic peak indicating the synthesis of copper nanoparticles. The pattern of XRD analysis showed particle size of 16 - 25 nm and it reveals high crystallinity of the copper nanoparticles. Zeta potential was done to find the charge of the nanoparticles and size distribution which showed to have significant stability. This method proves to be cost-effective, can be performed at ease, and it’s also free of pollutants.

scholarly journals A COMPARATIVE IN VITRO ANTI-BIOFILM EFFICACY OF PHYTOSYNTHESIZED IRON AND SILVER NANOPARTICLES

2019 ◽  
pp. 72-76
Author(s):  
S. GOKUL BRINDHA ◽  
V. S. HANSIYA ◽  
P. UMA MAHESWARI ◽  
N. GEETHA
Keyword(s):  
Colour Change ◽  
Single Step ◽  
Ring Test ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
Leaf Extracts ◽  
Synthesis Of Nanoparticles ◽  
Biofilm Destruction ◽  
Moringa Oleifera Lam

Objective: The objective of the work was to evaluate the efficacy of anti-biofilm activity of green synthesized silver and iron oxide nanoparticles comparatively. Methods: Nanoparticles were synthesized using a rapid, single-step and completely by a green biosynthetic method employing aqueous leaf extracts of Moringa oleifera Lam. The synthesized nanoparticles were characterized by UV-Vis spectrophotometry and X-Ray Diffraction. Bacterial strains used in this study included Staphylococcus epidermidis and Pseudomonas aeruginosa. The biofilm reduction was evaluated through ring test using crystal violet as a staining agent. Results: Colour change was observed after half an hour, which indicated the formation of silver and iron nanoparticles. Synthesis of nanoparticles was confirmed by UV and XRD. The anti-biofilm forming ability of AgNPs and FeNPs were compared with standard antibiotic. It was found that FeNPs showed more biofilm destruction ability (58%) for S. compared to P. aeruginosa (50%) and standard (30%). Whereas, AgNPs displayed significant biofilm destruction ability (78%) for P. aeruginosa compared to S. epidermidis (43%) and standard (34%). Conclusion: Based on the results obtained in this investigation, it is concluded that FeNPs have anti-biofilm activity against S. epidermidis whereas AgNPs have anti-biofilm activity against P. aeruginosa.

Novel dithranol loaded cyclodextrin nanosponges for augmentation of solubility, photostability and cytocompatibility

2020 ◽  
Vol 16 ◽  
Author(s):  
Sunil Kumar ◽  
Rekha Rao
Keyword(s):  
In Vitro Release ◽  
X Ray Diffraction ◽  
Standard Drug ◽  
Molar Ratios ◽  
Rational Engineering ◽  
Cyclodextrin Nanosponges ◽  
Vitro Release ◽  
Poor Stability

Background: Drug loaded β-cyclodextrin based nanosponges (CDNS) are of special interest for the entrapment of moieties with the view to address their physicochemical challenges, and to improve their delivery characteristics and utility. Dithranol (DTH), the standard drug for psoriasis, has poor stability and solubility, which limits its pharmaceutical applications. Objective: The objective of the current study was to entrap DTH in CDNS in order to alleviate the above-mentioned challenges. Methods: To synthesize CDNS, β-cyclodextrin was treated with diphenyl carbonate in various molar ratios. The obtained placebo CDNS were loaded with DTH by lyophilisation. The particle size of the DTH loaded CDNS was found to lie between 150 and 450 nm, with narrow polydispersity index range. Fourier transform infrared spectroscopy, thermal analysis, X-ray diffraction, zeta potential and electron microscopy with energy dispersive spectroscopy (EDS) were conducted for characterization of DTH-CDNS. Results: Findings from spectral examinations confirmed the formation of inclusion complexes. Solubilisation efficiency of DTH (in distilled water), was found augmented 4.54 folds with optimized CDNS. The cytocompatibility study was performed by the MTT assay employing THP1 cell lines. A remarkable amelioration in stability and photostability of DTH was also observed, by its inclusion in nanosponges. Conclusion: In nutshell, we report the rational engineering and characterization of DTH loaded cyclodextrin-based nanosponges, and subsequently, their stepwise screening for photostability, in vitro release, in vitro cytocompatibility, in vitro antioxidant and in vitro inflammatory activity in a top-down manner, yielding the best carrier for this drug.

scholarly journals Green Synthesis, Characterization and Cholinesterase Inhibitory Potential of Gold Nanoparticles

2021 ◽  
Vol 65 (3) ◽  
Author(s):  
Zainab ◽  
Khalid Saeed ◽  
Ammara ◽  
Shujaat Ahmad ◽  
Hanif Ahmad ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Green Synthesis ◽  
X Ray Diffraction ◽  
Au Nps ◽  
X Ray ◽  
Sem Study ◽  
Gold Nps ◽  
Uv Visible ◽  
Inhibitory Potential

Abstract. The green synthesis of gold nanoparticles (Au NPs) from their precursor was carried out using Delphinium uncinatum and Erythrophyleum guineense plants extracts. The Au NPs obtained were characterized by various instrumental techniques such as scanning electron microscopy (SEM), energy dispersive X-ray (EDX), X-ray diffraction (XRD) and UV/Visible (UV/VIS) spectrophotometer. The SEM study presented that E. guineense (GE) and D. uncinatum (GN) synthesized gold nanoparticles was below 100 and 300 nm, respectively.  The micrographs also presented that E. guineense (GE) synthesized gold particles had irregular round shaped while the D. uncinatum (GN) synthesized nanoparticles had cylindrical shaped. The XRD spectra presented peaks at about 38.1°, 44.43°, 64.6° and 77.64° can be indexed to (111), (200), (220) and (311) orientation, respectively, which confirmed the presence of gold nanoparticles. It means that both E. guineense (GE) and D. uncinatum (GN) synthesized gold nanoparticles are highly crystalline.  The UV/VIS analysis presented that both plant extracts significantly reduced the gold slat and as a result high quantity of gold nanoparticles were formed. The E. guanense gold NP and D. uncinatum gold NPs were investigated for their in-vitro cholinesterases inhibitory potentials in 62.5-1000 µg/mL concentrations range. The bioactivity results presented that the loading of the test samples in gold NPs enhanced their AChE and BChE inhibitory potentials.   Resumen. La síntesis verde de nanopartículas de oro (NP de Au) se llevó a cabo mediante extractos de plantas de Delphinium uncinatum y Erythrophyleum guineense. Las NP de Au obtenidas se caracterizaron mediante diversas técnicas instrumentales como microscopía electrónica de barrido (SEM), dispersión de energía de rayos X (EDX), difracción de rayos X (XRD) y espectrofotómetro UV / Visible (UV / Vis). El estudio SEM reveló tamaños de las nanopartículas de oro sintetizadas por E. guineense (GE) y D. uncinatum (GN) por debajo de 100 y 300 nm, respectivamente. Las micrografías también mostraron que las partículas de oro sintetizadas por E. guineense (GE) tenían una forma redonda irregular, mientras que las nanopartículas sintetizadas por D. uncinatum (GN) tenían una forma cilíndrica. Los patrones XRD presentaron picos a aproximadamente 38.1 °, 44.43 °, 64.6 ° y 77.64 ° pueden indexarse ​​a la orientación (111), (200), (220) y (311), respectivamente, lo que confirmó la presencia de nanopartículas de oro cristalinas. El análisis UV / Vis mostró que ambos extractos de plantas formaron nanopartículas de oro. Se investigaron las NP de oro como inhibidores de colinesterasas in vitro en un intervalo de concentraciones de 62.5 a 1000 µg / ml. Los resultados de la bioactividad mostraron que la carga de las muestras de prueba en NP de oro mejoró sus potenciales inhibidores de AChE y BChE.

scholarly journals Green Synthesis of Nickel Oxide Nanoparticles from Berberis balochistanica Stem for Investigating Bioactivities

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1548
Author(s):  
Siraj Uddin ◽  
Luqman Bin Safdar ◽  
Saeed Anwar ◽  
Javed Iqbal ◽  
Sabiha Laila ◽  
...  
Keyword(s):  
Nickel Oxide ◽  
Green Synthesis ◽  
Antioxidant Activities ◽  
Germination Rate ◽  
Oxide Nanoparticles ◽  
X Ray Diffraction ◽  
Nickel Oxide Nanoparticles ◽  
Total Antioxidant ◽  
Elemental Nature

Green synthesis of nanomaterials is advancing due to its ease of synthesis, inexpensiveness, nontoxicity and renewability. In the present study, an eco-friendly biogenic method was developed for the green synthesis of nickel oxide nanoparticles (NiONPs) using phytochemically rich Berberis balochistanica stem (BBS) extract. The BBS extract was rich in phenolics, flavonoids and berberine. These phytochemicals successfully reduced and stabilised the NiNO3 (green) into NiONPs (greenish-gray). BBS-NiONPs were confirmed by using UV-visible spectroscopy (peak at 305 nm), X-ray diffraction (size of 31.44 nm), Fourier transform infrared spectroscopy (identified -OH group and Ni-O formation), energy dispersive spectroscopy (showed specified elemental nature) and scanning electron microscopy (showed rhombohedral agglomerated shape). BBS-NiONPs were exposed to multiple in vitro bioactivities to ascertain their beneficial biological applications. They exhibited strong antioxidant activities: total antioxidant capacity (64.77%) and 2, 2-diphenyl-1-picrylhydrazyl (71.48%); and cytotoxic potential: Brine shrimp cytotoxicity assay with IC50 (10.40 µg/mL). BBS-NiONPs restricted the bacterial and fungal pathogenic growths at 1000, 500 and 100 µg/mL. Additionally, BBS-NiONPs showed stimulatory efficacy by enhancing seed germination rate and seedling growth at 31.25 and 62.5 µg/mL. In aggregate, BBS extract has a potent antioxidant activity which makes the green biosynthesis of NiONPs easy, economical and safe. The biochemical potential of BBS-NiONPs can be useful in various biomedical and agricultural fields.

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