scholarly journals ANTIOXIDANT STUDIES OF ONE AYURVEDIC MEDICINE ASWAGANDHARISHTAM

2018 ◽  
Vol 11 (11) ◽  
pp. 227
Author(s):  
Kotteswari M ◽  
Mudiganti Ram Krishna Rao ◽  
Prabhu K ◽  
Siva Kumar ◽  
Sampad Shil
Keyword(s):  
Ascorbic Acid ◽  
Sulfonic Acid ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Ayurvedic Medicine ◽  
Abts Assay ◽  
Ic50 Values ◽  
Reducing Power Assay ◽  
Liquid Medicine

Objective: The present study deals with the antioxidant study of one Ayurvedic medicine Aswagandhrishtam by three different methods, namely reducing power, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays.Methods: Aswagandharishtam, which is a liquid medicine, was taken as such at various concentrations for all the three assays.Results: The results show that Aswagandharistam has good antioxidant potential when compared with ascorbic acid as standard. The IC50 values of reducing power assay were 250.142, that of DPPH were 103.607, and of ABTS assay were 197.79 as compared with that of ascorbic acid being 19.59.Conclusion: All the three assays indicated that Aswagandharishtam showed very good antioxidant results.

scholarly journals Antioxidant activity of fungi isolated from soil of different areas of Punjab,India

2009 ◽  
Vol 1 (2) ◽  
pp. 123-128 ◽  
Author(s):  
Priyanka Chandra ◽  
Daljit Singh Arora
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Higher Plants ◽  
Reducing Power ◽  
Natural Antioxidants ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Reducing Power Assay ◽  
Set Up

The study was carried outto investigate the antioxidant activity of fungi isolated from soil of different areas of Punjab, India and compare their efficiency with a known antioxidant, ascorbic acid. The antioxidant potential of fungal extracts was quantified by DPPH and reducing power assay. Total phenolic contents were estimated using Folins-Ciocalteau (FC) reagent. Out of 113 fungal isolates selected, 51 were having antioxidant potential and these were further assayed quantitatively. All of these showed good activity against DPPH radical while 32 of these isolates demonstrated reducing potential also. In addition, some were equally good as ascorbic acid. The present study demonstrated potential of soil fungi to have antioxidant activity similar to plants and mushrooms. High phenolic content of fungi further highlight their significance as new sources of natural antioxidants. These fungi may provide easier set up for production and purification of natural antioxidants as compared to higher plants.

In Vitro Free Radical Scavenging Activity and Total Flavonoid of Abelmoschus manihot (L.) Medic Extracts

2011 ◽  
Vol 140 ◽  
pp. 355-359 ◽  
Author(s):  
Yan Qiu ◽  
Jian Jun Song
Keyword(s):  
Ascorbic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Flavonoid ◽  
Scavenging Activity ◽  
Ic50 Values ◽  
Hydrogen Radicals ◽  
Dpph Radical Scavenging

The Ablmoschus manihot (L.) Medic, an edible hibiscus of the Malvaceae family, is abundant with total flavonoid. The content of total flavonoid from A. manihot flowers (TFA) was extracted by 70% ethanol (ethanol:water, 70:30). The scavenging effects of TFA on superoxide anions and hydrogen radicals were related to the total flavonoid concentrations with the IC50 values of 63.90 ±2.21 μg/mL and 266.88±28.32 μg/mL, respectively. However, the IC50 values of standard ascorbic acid were 436.52 ±14.36 μg/mL and 439.58±21.41 μg/mL, respectively. The DPPH radical scavenging activity increased with increasing of TFA concentrations and the highest inhibition was 94.63 ±3.01% at 50 μg/mL, as compared to ascorbic acid 34.94 ±1.50%. The extract also showed good reducing power. The data from this study suggest A. manihot flower extract has significant potential to use as health supplements and nutraceuticals.

scholarly journals PHYTOCHEMICAL AND ANTIOXIDANT ACTIVITY OF CAMELLIA MISTLETOE (KORTHALSELLA JAPONICA) EXTRACTS

2017 ◽  
Vol 10 (12) ◽  
pp. 400
Author(s):  
Min Young Kim
Keyword(s):  
Ascorbic Acid ◽  
Reducing Power ◽  
Ethanol Extract ◽  
In Vitro Models ◽  
Total Flavonoid ◽  
Total Carotenoid ◽  
Ic50 Values ◽  
Mistletoe Extracts ◽  
Ethanol Extracts

Objective: This study was aimed to analyze the methanol or ethanol extract of Camellia mistletoe (Korthalsella japonica) growing in Jeju Island, Korea, for their composition of phenolics, flavonoids, and their antioxidant activity.Methods: Total flavonoid, total carotenoid and L-ascorbic acid contents were determined by using colorimetric assays, and the antioxidant attributes of the methanol and Camellia mistletoe extracts were assayed using in vitro models, including the following: Free radical (1,1-diphenyl-2-picrylhydrazyl, O2-• and NO•)scavenging, reducing power, and chelating ferrous ions.Results: Total flavonoid, total carotenoid and L-ascorbic acid contents were 20.3 and 14.9 mg RE/100 g, 4.4 and 1.7 mg/100 g, and 0.4 and 1.4 mg AA/100 g in the methanol and ethanol extracts of Camellia mistletoe, respectively. Based on IC50 values, both extracts were also found to possess potent antioxidant activities.Conclusions: Camellia mistletoe extracts could be the good antioxidant source and holds promise as a natural ingredient in functional food and pharmaceutical supplement.

scholarly journals Evaluation of Ethnopharmacological and Antioxidant Potential ofZanthoxylum armatumDC.

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Rabia Kanwal ◽  
Muhammad Arshad ◽  
Yamin Bibi ◽  
Saira Asif ◽  
Sunbal Khalil Chaudhari
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Zanthoxylum Armatum

Zanthoxylum armatumDC. (syn.Z. alatumRoxb.) is an important medicinal plant commonly called Timur or Indian prickly ash. The ethnopharmacological study ofZ. armatumrevealed the use of different plant parts for curing various ailments including cholera, chest infection, fever, indigestion, stomach disorders, gas problems, piles, toothache, gum problems, dyspepsia, as carminative, antipyretic, aromatic, tonic, and stomachic. Keeping in view the medicinal potential of the plant, the antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, reducing power, and phosphomolybdate assay using different concentrations (7.81 μg/mL–250 μg/mL). Ascorbic acid was taken as standard. The results indicated that the free radical scavenging activity ranged from 40.12% to 78.39%, and the reductive potential ranged from 0.265 nm to 1.411 nm while the total antioxidant activity ranged from 0.124 nm to 0.183 nm. The antioxidant potential evaluated by three assays increased in a concentration dependent manner and ascorbic acid showed better antioxidant activity than leaf extract. Results obtained through different tests confirmed redox protective activities ofZanthoxylum armatum. Further in vitro and in vivo research should be performed, so this plant can be further utilized in drug development.

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY AND FOURIER TRANSFORM INFRARED ANALYSIS OF ELAEAGNUS CONFERTA ROXB. LEAF EXTRACT

2020 ◽  
pp. 50-53
Author(s):  
LALITHA G ◽  
NAZEEMA TH
Keyword(s):  
Ascorbic Acid ◽  
Fourier Transform ◽  
Reducing Power ◽  
Fourier Transform Infrared ◽  
Antioxidant Potential ◽  
Primary Amines ◽  
Ftir Analysis ◽  
Characteristic Peak ◽  
Antioxidant Power ◽  
Ic50 Value

Objective: The objective of this study was to carry out the antioxidant potential of Elaeagnus conferta (EC) Roxb. leaves that were worked on various assay 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation decolorization, total reducing power was studied by ferric reducing antioxidant power (FRAP) and total radical trapping antioxidant potential (TRAP) method. The spectroscopic technique Fourier transform infrared (FTIR) spectrophotometer was performed to detect the characteristic peak values and their functional groups of EC Roxb. Methods: Ethanol solvent is used ABTS, DPPH, FRAP, TRAP, and FTIR analysis. Results: ABTS assay, IC50 value was found to be 60 μg/ml compared with standard ascorbic acid 64 μg/ml. DPPH IC50 value of EC Roxb. compared with standard ascorbic acid was found to be 0.43 μg/ml and 0.62 μg/ml. FRAP IC50 value along with standard ascorbic acid was found to be 0.582 μg/ml and 0.596 μg/ml. The IC50 value of TRAP along with standard ascorbic acid was found to be 0.39 μg/ml and 0.62 μg/ml. In FTIR spectroscopic study, it revealed different characteristic peak values with various functional compounds such as phenols, alcohols, amide, alkanes, carboxylic acids, alkenes, primary amines, aromatics, ester, alkyl halides, and aliphatic amines compounds. Conclusion: EC Roxb. leaves have a potent rich source of antioxidant. In FTIR analysis, it is confirmed with the presence of phenolic compounds that can be emphasized for efficacy in pharmaceutics.

scholarly journals Comparative Antioxidant Potential of Different Extracts of Celastrus paniculatus Willd. Seed

1970 ◽  
Vol 3 (1) ◽  
pp. 68-74 ◽  
Author(s):  
Fatema Tuz Zohera ◽  
Md Razibul Habib ◽  
Mohammad Zafar Imam ◽  
Md Ehsanul Hoque Mazumder ◽  
Md Sohel Rana
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Dependent Manner ◽  
Ic50 Value ◽  
Celastrus Paniculatus

The objective of the present study was to evaluate the comparative antioxidant potential of methanol, ethyl acetate, pet ether and water extracts of Celastrus paniculatus seed. Antioxidant activity was evaluated by using total phenol and flavonoid content determination assays, total antioxidant capacity, 1,1-diphenyl-2- picryl-hydrazil (DPPH) free radical assay, Reducing power assessment, Nitric oxide (NO) scavenging assay and Cupric ion reducing capacity assay (CUPRAC method). The extracts showed moderate antioxidant activity in a dose dependent manner. The extracts were found to contain phenolics and flavonoid compounds. In DPPH radical scavenging assay, ethyl acetate extract had the lowest IC50 value (585.58μg/ml) compared to ascorbic acid. In nitric oxide scavenging assay IC50 value was found to be 122.99μg/ml, 320.54μg/ml, 601.81μg/ml and 206.37μg/ml respectively for the Water, Methanol, Ethyl Acetate and Pet Ether extracts compared to 6.83μg/ml which was the IC50 value for the reference ascorbic acid. The extracts also showed good reducing power. The results of the present study indicate that the extracts possesses significant antioxidant potential of which ethyl acetate extract is the most promising one and possess highest antioxidant potential. Key Words: Celastrus paniculatus; antioxidant; DPPH; NO scavenging; CUPRAC; ROS. DOI: 10.3329/sjps.v3i1.6802S. J. Pharm. Sci. 3(1): 68-74

scholarly journals The effect of freezing and drying Cornus mas L. from phenols, flavonoids, anthocyanins, and total content of ascorbic acid and antioxidant activity in two methods

Biologija ◽  
2019 ◽  
Vol 65 (3) ◽  
Author(s):  
Marzieh Piryaei ◽  
Marzieh Babashpour Asl ◽  
Mir Mahdi Abolghasemi
Keyword(s):  
Ascorbic Acid ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Ultrasonic Method ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Content ◽  
Cornus Mas ◽  
Reducing Power Assay ◽  
Better Than

Flavonoids, anthocyanins, antioxidant capability, total content of phenolic and ascorbic acid of Cornus mas of frozen and dried for polar and non-polar subfraction were evaluated in this work. Antioxidant activities of the samples were determined by three testing systems, namely, DPPH, β-carotene/linoleic acid, and reducing power assay. In the DPPH system, the highest radical scavenging activity was seen in the polar subfraction of the methanol extract in frozen fruits (IC50 = 25.15 ± 6.5 µg mL–1). Comparison of two methods of extraction, maceration and the ultrasonic method, showed that the results of maceration are better than those of the ultrasonic method. These advantages are visible in extracting the flavonoids, anthocyanins, antioxidant capability, and the total content of phenolic and ascorbic acid.

Phytochemical analysis, total phenolic, total flavonoid contents and ferric reducing power of extracts from roots and leaves of Searsia burchellii

Food Research ◽  
2021 ◽  
Vol 5 (3) ◽  
pp. 241-248
Author(s):  
M.K. Pillai ◽  
M.S. Mpopo ◽  
S.B. Mekbib
Keyword(s):  
Ascorbic Acid ◽  
Reducing Power ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phenolic Contents ◽  
Methanolic Extracts ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Phytochemical Profiles

Searsia burchellii has therapeutic applications in traditional medicine. The aim of the present study was to analyse the phytochemical profiles, total phenolic contents (TPC), total flavonoid contents (TFC) and ferric reducing power of various extracts from roots and leaves of S. burchellii collected from the kingdom of Lesotho. A combination of maceration, hot solvent extraction and solvent-solvent partition techniques were used to obtain methanolic extracts, fractions such as hexane, chloroform, ethyl acetate and methanol/water from methanolic extracts and water extracts separately from the roots and leaves of S. burchellii. The presence of alkaloids, steroids, terpenoids, phenolics, tannins, flavonoids, coumarins, saponins, glycosides, carbohydrates, proteins and phlobatannins were identified from these extracts/fractions. The TPC of these extracts/fractions was found to be in the range of 3.22±0.37 to 247.46±4.33 mg TAE/g DW, respectively. The TFC of these extracts/fractions was found to be in the range of 34.70±4.31 to 278.20±2.01 mg QE/g DW, respectively. Additionally, these extracts/fractions showed a moderate to strong ferric reducing power compared to ascorbic acid. The IC50 values were found to be in the range of 5.35-143.12 and 65.64-348.28 µg/mL for extracts/fractions from the roots and leaves, respectively. Ascorbic acid showed an IC50 value of 20.93 µg/mL. Therefore, the extracts/fractions from S. burchellii have the potential to replace the existing synthetic antioxidants

Evaluation of Antioxidant Activity, Radical Scavenging, and Reducing Power of Clove Oil and Clove Oleoresin in Comparison with Natural and Synthetic Antioxidants in Chevon (Capra aegagrus hircus) and Chicken Meat

2017 ◽  
Vol 3 (1) ◽  
pp. 51 ◽  
Author(s):  
Khudsia Sultana ◽  
K. Jayathilakan ◽  
M. C. Pandey
Keyword(s):  
Ascorbic Acid ◽  
Radical Scavenging ◽  
Meat Products ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Refrigerated Storage ◽  
Clove Oil ◽  
Synthetic Antioxidants ◽  
Capra Aegagrus ◽  
Antioxidant Effects

<p>The antioxidant effects of clove oil (CO) and oleoresin (OR) in two species of meat i.e. chicken and chevon during refrigerated storage (4±1°C) were investigated. The antioxidant potential (AOP) and radical scavenging activities were compared with natural (α-Tocopherol &amp; <em>L</em>-ascorbic acid) and synthetic antioxidants (BHA &amp; TBHQ). CO &amp; TBHQ, BHA &amp; OR and L-ascorbic acid and α-Tocopherol produced 84-79%, 68-77% and 68-59% AOP respectively in both species. DPPH and ABTS methods of scavenging assay established better scavenging capability of CO and TBHQ in comparison with other treatments. Significant reduction (p&lt;0.05) in FRAP values was observed in CO &amp; TBHQ in both species of meat. The order of antioxidant potential, scavenging activity and reducing power was in the order of CO&gt;TBHQ&gt;OR&gt;BHA&gt;Tocopherol&gt;L-ascorbic acid. All the antioxidant assays analysed demonstrated a very significant correlation (p&lt;0.05) between each other. The results suggest that CO and OR through their antioxidant effects are potentially useful in preserving meat products.</p>

Synthesis, characterization, and investigation of the antioxidant activity of some 1,2,4-benzothiadiazine-1,1-dioxides bearing sulfonylthioureas moieties

2019 ◽  
Vol 97 (12) ◽  
pp. 824-832 ◽  
Author(s):  
Kamel Harrouche ◽  
Asma Lahouel ◽  
Mebrouk Belghobsi ◽  
Bernard Pirotte ◽  
Smail Khelili
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Hydrogen Atom ◽  
Crucial Role ◽  
Reducing Power ◽  
Methyl Groups ◽  
Methyl Group ◽  
The Future ◽  
Reducing Power Assay

A series of 1,2,4-benzothiadiazine-1,1-dioxides bearing a sulfonylthiourea moiety were synthesized, characterized, and screened for their antioxidant activity, using six antioxidant analytical assays comparatively to reference compounds, ascorbic acid and quercetin. The results indicated that several compounds demonstrated strong antioxidant activity in DPPH, ABTS, H2O2, and lipid peroxidation assays where some of them were either as active as or more active than reference compounds. However, all compounds were largely less active than references compounds in the reducing power assay. The results indicated that the thiourea moiety probably played a crucial role in the antioxidant activity of the target compounds, as a thiolate ion. The most favorable R1 groups were the hydrogen atom and methyl group, followed by phenyl and benzyl groups, whereas the most favorable R2 group was iPr, followed by the phenyl and methyl groups. The combination of benzothiadiazine ring with sulfonylthiourea moieties led to valuable new antioxidants, which could be used in the treatment or the prevention of certain diseases or in the field of cosmetics, which needs further investigations in the future.

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