scholarly journals A REVIEW: A GREEN APPROACH FOR THE SYNTHESIS OF SILVER NANOPARTICLES AND ITS ANTIBACTERIAL APPLICATIONS

Author(s):  
Shyla Marjorie Haqq ◽  
Amit Chattree

  This review is based on the synthesis of silver nanoparticles (AgNPs) using a green approach which is biofabricated from various medicinal plants. AgNPs were prepared from the various parts of the plants such as the flowers, stems, leaves, and fruits. Various physiochemical characterizations were performed using the ultraviolet (UV)-visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction spectroscopy, transmission electron microscopy, and energy dispersive spectroscopy. AgNPs were also used to inhibit the growth of bacterial pathogens and were found to be effective against both the Gram-positive and Gram-negative bacteria. For the silver to have antimicrobial properties, it must be present in the ionized form. All the forms of silver-containing compounds with the observed antimicrobial properties are in one way or another source of silver ions. Although the antimicrobial properties of silver have been known, it is thought that the silver atoms bind to the thiol groups in enzymes and subsequently leads to the deactivation of enzymes. For the silver to have antimicrobial properties, it must be present in the ionized form. The study suggested that the action of the AgNPs on the microbial cells resulted into cell lysis and DNA damage. AgNPs have proved their candidature as a potential antibacterial against the multidrug-resistant microbes. The biological agents for synthesizing AgNPs cover compounds produced naturally in microbes and plants. Reaction parameters under which the AgNPs were being synthesized hold prominent impact on their size, shape, and application. Silver nanoparticle synthesis and their application are summarized and critically discussed in this review.

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Edwina Olohirere Uzunuigbe ◽  
Abidemi Paul Kappo ◽  
Sixberth Mlowe ◽  
Neerish Revaprasadu

Synthesizing nanoparticles with the less environmentally malignant approach using plant extract is of great interest; this is because most of the chemical approaches can be very costly, toxic, and time-consuming. Herein, we report the use of Acacia senegal leaf extracts to synthesize silver nanoparticles (AgNPs) using an environmentally greener approach. Silver ions were reduced using the bioactive components of the plant extracts with observable colour change from faint colourless to a brownish solution as indication of AgNP formation. The structural properties of the as-synthesized AgNPs were characterized using powder X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and UV-Vis absorption spectrum. Antimicrobial assessment of the as-synthesized AgNPs was explored on some strains of gram-positive and gram-negative bacteria. The obtained results indicate that the as-synthesized AgNPs are pure crystallite of cubic phase of AgNPs, fairly dispersed with a size range of 10–19 nm. The AgNPs were found to be small in size and exhibit significant antibacterial activities, suggesting that the as-synthesized AgNPs could be used in the pharmaceutical and food industries as bactericidal agents.


2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Deene Manikprabhu ◽  
K. Lingappa

Traditional synthesis of silver nanoparticles using chemical methods produces toxic substances. In contrast biological synthesis is regarded as a safe and nontoxic process but the major drawback of biological synthesis is, this process is slow. In the present investigation, we developed a rapid and green synthesis of silver nanoparticles employing a pigment produced byStreptomyces coelicolorklmp33 in just 90 s. The silver nanoparticles were characterized by UV-visible spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The biobased synthesis developed in this method is a safe, rapid, and appropriate way for bulky synthesis of silver nanoparticles.


2016 ◽  
Vol 5 (6) ◽  
Author(s):  
Brajesh Kumar ◽  
Kumari Smita ◽  
Luis Cumbal

AbstractThe present report summarizes an eco-friendly approach for the biosynthesis of silver nanoparticles (AgNPs) using the leaf extract of lavender. Initially, the synthesis of AgNPs was visually observed by the appearance of a wine red color. The optical property, morphology, and structure of as-synthesized AgNPs were characterized by UV-visible spectroscopy, dynamic light scattering, transmission electron microscopy, and X-ray diffraction analyses. All characterization data revealed the formation of crystalline and spherical AgNPs (Ag/Ag


2011 ◽  
Vol 109 ◽  
pp. 174-177 ◽  
Author(s):  
Yu Li Shi ◽  
Qi Zhou ◽  
Li Yun Lv ◽  
Wang Hong

A facile method for the synthesis of silver nanoparticles (NPs) has been developed by using sodium phosphate (Na3PO4) as stabilizing agents and glucose the reducing agent, respectively. The obtained silver NPs have been characterized by X-ray diffraction (XRD), UV-visible spectroscopy (UV-vis) and transmission electron microscopy (TEM). It was found that in the presence of sodium phosphate, silver NPs with different morphologies and sizes were obtained. The formation mechanism of diverse silver NPs was studied preliminarily.


Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7325
Author(s):  
Denisa Batir-Marin ◽  
Cornelia Mircea ◽  
Monica Boev ◽  
Ana Flavia Burlec ◽  
Andreia Corciova ◽  
...  

The ethanolic extracts of three Equisetum species (E. pratense Ehrh., E. sylvaticum L. and E. telmateia Ehrh.) were used to reduce silver ions to silver nanoparticles (AgNPs). The synthesized AgNPs were characterized using UV-Vis spectrophotometry, Fourier Transform Infrared Spectroscopy (FTIR), Energy Dispersive X-ray (EDX), Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS) measurements. FTIR data revealed the functional groups of biomolecules involved in AgNPs synthesis, such as O-H, C-H, C=O, C-O, and C-C. EDX spectroscopy was used to highlight the presence of silver, while DLS spectroscopy provided information on the mean diameter of AgNPs, that ranged from 74.4 to 314 nm. The negative Zeta potential values (−23.76 for Ep–AgNPs, −29.54 for Es–AgNPs and −20.72 for Et–AgNPs) indicate the stability of the obtained colloidal solution. The study also focused on establishing the photocatalytic activity of AgNPs, which is an important aspect in terms of removing organic dyes from the environment. The best photocatalytic activity was observed for AgNPs obtained from E. telmateia, which degraded malachite green in a proportion of 97.9%. The antioxidant action of the three AgNPs samples was highlighted comparatively through four tests, with the best overall antioxidant capacity being observed for AgNPs obtained using E. sylvaticum. Moreover, the biosynthesized AgNPs showed promising cytotoxic efficacy against cancerous cell line MG63, the AgNPs obtained from E. sylvaticum L. providing the best result, with a LD50 value around 1.5 mg/mL.


Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4479
Author(s):  
Pei-Jun Li ◽  
Jiang-Juan Pan ◽  
Li-Jun Tao ◽  
Xia Li ◽  
Dong-Lin Su ◽  
...  

The present study focuses on the biological synthesis, characterization, and antibacterial activities of silver nanoparticles (AgNPs) using extracellular extracts of Aspergillus japonicus PJ01.The optimal conditions of the synthesis process were: 10 mL of extracellular extracts, 1 mL of AgNO3 (0.8 mol/L), 4 mL of NaOH solution (1.5 mol/L), 30 °C, and a reaction time of 1 min. The characterizations of AgNPs were tested by UV-visible spectrophotometry, zeta potential, scanning electron microscope (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and thermogravimetric (TG) analyses. Fourier transform infrared spectroscopy (FTIR) analysis showed that Ag+ was reduced by the extracellular extracts, which consisted chiefly of soluble proteins and reducing sugars. In this work, AgNO3 concentration played an important role in the physicochemical properties and antibacterial properties of AgNPs. Under the AgNO3 concentration of 0.2 and 0.8 mol/L, the diameters of AgNPs were 3.8 ± 1.1 and 9.1 ± 2.9 nm, respectively. In addition, smaller-sized AgNPs showed higher antimicrobial properties, and the minimum inhibitory concentration (MIC) values against both E. coli and S. aureus were 0.32 mg/mL.


2018 ◽  
Vol 83 (5) ◽  
pp. 515-538 ◽  
Author(s):  
Andreia Corciova ◽  
Bianca Ivanescu

Nanotechnology is one of the most studied domains, and nanoparticle synthesis, especially of silver nanoparticles, has gained special importance due to their properties, biocompatibility and applications. Today, the processes of nanoparticles synthesis tend toward the development of inexpensive, simple, non-toxic and environmentally friendly methods. Thus, the use of plants in the synthesis of silver nanoparticles has attracted considerable interest because biomolecules can act as both reducing and stabilizing agents. This survey aims at discussing the conditions for obtaining silver nanoparticles using plants and their characterization by several methods, such as FTIR and UV?Vis spectroscopy, X-ray diffraction, and scanning and transmission electron microscopy. In addition, it examines some of the most common biological uses of silver nanoparticles: antibacterial, antioxidant and cytotoxic.


2020 ◽  
Vol 16 ◽  
Author(s):  
Arfaa Sajid ◽  
Qaisar Manzoor ◽  
Anam Sajid ◽  
Muhammad Imran ◽  
Shanza Khalid ◽  
...  

Background:: Currently, developing methods for the formation of nanoparticles with antimicrobial properties based on green chemistry are the research hotspots. In this research green biosynthesis of Eriobotrya japonica extract loaded silver nanoparticles and their characterization were the main objectives to achieve. Methods:: Green synthesis of E. japonica leaves extract-loaded silver nanoparticles (AgNPs) was carried out and its effect on bacterial growth was examined. The reduction of silver ions in solution was observed using UV-Vis spectrophotometer. The properties of AgNPs were assessed using Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). Also, their antibacterial effects were checked against Staphylococcus aureus and Escherichia coli. Results:: It was revealed that 5-50 nm sized spherical to elongated nanoparticles were synthesized that possessed comparatively better antibacterial potential against E. coli and S. aureus than conventional extract of the E. japonica leaves. Conclusions:: Green synthesis and effective utilization of Eriobotrya japonica extract loaded silver nanoparticles is a promising approach for nanoparticle production avoiding negative environmental impacts.


2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Palem Ramasubba Reddy ◽  
Shimoga D. Ganesh ◽  
Nabanita Saha ◽  
Oyunchimeg Zandraa ◽  
Petr Sáha

Bioreduction of silver ions following one pot process is described to achieveRheum rhabarbarum(RR) based silver nanoparticles (SNPs) which is termed as “RR-SNPs.” The Ultraviolet–visible spectroscopy (UV–vis) confirms the characteristic surface plasmon resonance band for RR-SNPs in the range of 420–460 nm. The crystalline nature of SNPs was confirmed by X-ray diffraction (XRD) peaks at 38.2°, 45.6°, 64.2°, and 76.8°. Transmission electron microscopy (TEM) and scanning electronic microscopy (SEM) confirm the shape of synthesized SNPs. They are roughly spherical but uniformly distributed, and size varies from 60 to 80 nm. These biogenic SNPs show persistent zeta potential value of 34.8 mV even after 120 days and exhibit potent antibacterial activity in presence ofEscherichia coli(CCM 4517) andStaphylococcus aureus(CCM 4516). In addition, cytotoxicity of RR-SNPs againstin vitrohuman epithelial carcinoma (HeLa) cell line showed a dose-response activity. The lethal concentration (LC50) value was found to be 28.5 μg/mL for RR-SNPs in the presence of HeLa cells. These findings help us to evaluate their appropriate applications in the field of nanotechnology and nanomedicine.


2015 ◽  
Vol 1086 ◽  
pp. 11-30 ◽  
Author(s):  
Chellasamy Panneerselvam ◽  
Kadarkarai Murugan ◽  
Duraisamy Amerasan

Metallic nanoparticles have received great attention from chemists, physicists, biologists and engineers who wish to use them for the development of a new generation of nanodevices. In the present Communication, a completely “green” chemistry method for producing silver nanoparticles is introduced. The process is simple, environmentally benign, and quite efficient. Green nanoparticle synthesis has been achieved using environmentally acceptable plant extract and ecofriendly reducing and capping agents. In particular, silver nanoparticles are proved to have potential antibacterial, antifungal and antiplasmodial and antimicrobial properties. The present study was aimed to identify the antiplasmodial activity of green synthesised silver nanoparticles (AgNPs) using aqueous extract of plantEuphorbia hirtaagainstP.falciparum. Nanoparticles are being used in many commercial applications. It was found that aqueous silver ions can be reduced by aqueous extract of plant to generate extremely stable silver nanoparticles in water. The bio-reduced silver nanoparticles were appropriately characterized by UV–vis spectrum, Scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX) and X-ray diffraction (XRD). The formation of the AgNPs synthesized from the XRD spectrum compared with the standard confirmed spectrum of silver particles formed in the present experiments were in the form of nanocrystals, as evidenced by the peaks at 2θ values of =28.01°, 32.41°, 46.44°, 55.05° and 57.75°. The scanning electron micrograph (SEM) showed structures of spherical, cubic shape, and the size range was found to be 30–60 nm. The EDX spectra showed the purity of the material and the complete chemical composition of the synthesized AgNPs. The parasitic inhibition was dose-dependent. The synthesized AgNPs showed considerable antiplasmodial activity than the crude methanol and aqueous leaf extract ofE.hirta. The maximum efficacy was


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