scholarly journals DRY EXTRACT OF BLACK RICE (ORYZA SATIVA L.) AS ANTIOXIDANT IN THE FORM OF FUNCTIONAL DRINK

2018 ◽  
Vol 11 (13) ◽  
pp. 162
Author(s):  
Erlindha Gangga ◽  
Qurratun Aini

Objective: Based on previous research, black rice (Oryza sativa L.) has been proven to have antioxidant activity which is classified in the very strong category, approximately 41.5 bpj. this The aim of this study was to produce antioxidant functional drink.Methods: Simplicia powder prepared from black rice (O. sativa L.) sample by extracting with 70% ethanol solvent using kinetic maceration, followed by phytochemical screening, determination of extract quality, drying extract with freeze-drying method, then dry extract obtained used to produce functional drinks on which evaluation and antioxidant activity test carried out. The antioxidant activity test used in this research was DPPH-free radical scavenging method.Results: Screening result showed that both simplicia and extract contain flavonoid compounds, saponins, tannins, steroids, and triterpenoids. Result from dried extract quality determination shown to be thick consistency, has blackish-purple color, has specific aroma and bitter, water-soluble extract content of 59.04%; ethanol-soluble extract content of 69.77%; moisture content of 7.65%; loss on drying 9.40%; total ash content of 5.17%; acid-insoluble ash content of 0.84%; water-soluble ash content 4.15%; and residual solvent 0.44%. Pb and Cd metal contamination were 1.1994 mg/kg and 0.0905 mg/kg, respectively. Total plate count microbial contamination TNTC and the number of yeast and mold found to be 0.0387×103 colony/g. Evaluation test of functional drinks with powder type and found to be blackish-purple, and specific aroma, and total plate count microbial contamination was 0.310×103 colony/g, and the number of yeast and mold found was 0.6997×102 colony/g. Result of IC50 antioxidant activity of viscous extract was 37.5587 bpj; dried extract was 231.0101 bpj; dried extract (distilled water) was 44.7412 bpj; and functional drinks were 46.3594 bpj.Conclusion: Thus, results suggest that dried black rice’s extract and functional drink produced found to have very strong antioxidant activity.

2020 ◽  
Vol 3 (4) ◽  
pp. 209-215
Author(s):  
Sutomo Sutomo ◽  
Dina Noor Kamali ◽  
Arnida Arnida ◽  
Normaidah Normaidah ◽  
Agung Sriyono

Mundar (Garcinia forbesii King.) is a plant from South Kalimantan. This plant has chemical contents that have potential as medicine. The purpose of this study is to provide a pharmacognostic picture of a specific, nonspecific and determine the antioxidant activity of G. forbesii leaves. Specific parameters include organoleptic, microscopic testing, thin-layer chromatography profiles, and phytochemical screening. Nonspecific parameters include total ash content, acid insoluble ash content, drying loss, water-soluble extract content, and ethanol-soluble extract content. Antioxidant activity was determined by the DPPH method based on IC50 values. Specific parameter test results are green powder, characteristic odor, and sour taste. Microscopic tests showed stomata, upper epidermis, lower epidermis, cell walls, xylem, phloem, palisade tissue, spongy tissue, and cuticles. Garcinia forbesii leaves contain alkaloids, flavonoids, phenols, tannins, and steroids. The TLC profile showed good separation of polar eluents, and yellow spots appeared after spraying DPPH reagents. The non-specific parameter test is the total ash content of 6.52�0.1%; acid insoluble ash content 1.06�0.08%; drying shrinkage 6.43�0.38%; water-soluble extracts 34.3�0.3%; and ethanol-soluble extracts 23.47�0.35%. Ethanol extract of G. forbesii leaves has antioxidant activity with IC50 of 65.7 ppm. Pharmacognostic study fulfills the requirements, and G. forbesii leaves extract has strong antioxidant activity.


KOVALEN ◽  
2020 ◽  
Vol 6 (3) ◽  
pp. 184-190
Author(s):  
Eka Siswanto Syamsul ◽  
Supomo ◽  
Siti Jubaidah

Research on red Pidada leaves (Sonneratia caseolaris L.) which is a mangrove plant with antioxidant potential has been carried out. This study aims to determine the simplicia characterization and activity of red Pidada leaf extracts and fractions as antioxidants. The results of the simplicia characterization were: water-soluble extract content of 11%, ethanol-soluble extract content of 16%, water content of 9%, ash content of 8.17%, and acid insoluble ash content of 0.48%. The antioxidant activity test was carried out using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The antioxidant activity was determined by the IC50 (Inhibition Concentration) value. The results showed that the ethanol extract had an IC50 value of 24.22 ppm (very strong), n-hexane fraction 88.18 ppm (strong), ethyl acetate fraction 15.39 ppm (very strong), and ethanol fraction 38.41 ppm (very strong) Keywords: Sonneratia caseolaris L., antioxidants, DPPH, IC50


Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3389 ◽  
Author(s):  
Iuliana Aprodu ◽  
Ștefania Adelina Milea ◽  
Roxana-Mădălina Anghel ◽  
Elena Enachi ◽  
Vasilica Barbu ◽  
...  

The aqueous anthocyanin-rich extract derived from black rice (Oryza sativa L.) was encapsulated by freeze drying using milk proteins and peptides as coating materials. The molecular modelling approach indicated that all major casein fractions and whey proteins were able to bind at least one anthocyanin molecule. The hydrophobic interactions and hydrogen bonding across the interfaces appeared to be mainly responsible for the stabilizations of the complexes formed between the coating material and bioactive compounds. Two dark purple colored powders, differentiated by the ratio of the encapsulation materials used, rich in phytochemicals were obtained, with an encapsulation efficiency of up to 99%. The powders were tested for antioxidant activity, cytocompatibility, and thermal stability. The morphological structure of the powders highlighted the presence of encapsulated anthocyanins. Both powders showed a remarkable antioxidant activity of about 46 mM Trolox/g D.W., and cytocompatibility on the L929 fibroblast culture. At certain concentrations, both powders stimulated cell proliferation. The powders showed a good thermal stability between 75 and 100 °C for 15 min. The powders were tested in a food model system and checked for stability of phytochemicals during storage. The added value of the powders was demonstrated throughout the antioxidant activity, which remained unchanged during storage.


2010 ◽  
Vol 5 (3) ◽  
pp. 331-337 ◽  
Author(s):  
Neda Lakić ◽  
Neda Mimica-Dukić ◽  
Jelena Isak ◽  
Biljana Božin

AbstractThe antioxidant properties of methanol extracts of Lady’s Bedstraw (Galium verum L., Rubiaceae) herb from two different localities in Serbia were evaluated. Antioxidant activity was assessed in four different model systems. Free radical scavenging capacity (RSC) was examined by measuring the scavenging activity of extracts on 2,2-diphenyl-1-pycrylhydrazil (DPPH) and hydroxyl radical (OH), as well as on hydrogen peroxide. In addition, the protective effects of lipid peroxidation (LP) in corn oil were evaluated by the TBA-assay using the Fe2+/ascorbate system of induction. The amount of dried extract, the content of total phenolics, flavonoids and chlorophylls was also determined. Extracts from both locations expressed very strong scavenger activity, reducing the DPPH⊙ (IC50=3.10 µg/mland 8.04 µg/ml) and OH radical formation (IC50=0.05 µg/ml and 0.54 µg/ml) and neutralising H2O2 (IC50=4.98 µg/ml and 3.80 µg/ml), in a dose dependant manner. Also, examined extracts showed notable inhibition of LP (IC50=11.69 µg/ml and 19.47 µg/ml). The observed differences in antioxidant activity could be partially explained by the levels of phenolics (2.44–4.65 mg and 4.57–5.16 mg gallic acid equivalents/g dry extract), flavonoids (6.38–10.70 µg and 15.56–17.96 µg quercetin equivalents/g dry extract) and chlorophylls in the investigated Lady’s Bedstraw extracts.


2021 ◽  
Vol 12 (2) ◽  
pp. 131-140
Author(s):  
Zamharira Muslim ◽  
Heti Rais Khasanah ◽  
Yopita Sari

Trembesi plant (Samanea saman) is a plant that has the potential as traditional medicine. People in using medicinal plants often do not know the chemical content of these plants, so that in determining the amount of dosage, people only rely on experience and estimates. Research on the characterization of trembesi leaf simplicia has never been done. Research on the characterization of trembesi leaf simplicia has never been done. The purpose of this study was to determine the characteristics of trembesi leaf simplicia including specific and non-specific characteristics. The research is carried out in the form of experiments in the laboratory. The sample used is trembesi leaves. The research stages started from discovery, making simplicia, extraction, specific and non-specific characteristics. In the phytochemical test, ethanol extract of trembesi leaves was used. Data analysis used is descriptive method. Specific results showed trembesi leaf simplicia has a coarse powder form, distinctive odor, tasteless, green in color, and on microscopic results of trembesi leaf simplicia powder there is starch, fragments in the form of epidermis, water soluble extract content 11.93%, ethanol soluble essence 18, 93% and contains flavonoids, tannins, steroids and saponins. The results of non-specific characteristics are 8.07% water content, 7.91% ash content and 0.29% acid insoluble ash content.     


2020 ◽  
Vol 10 (4) ◽  
pp. 22-28
Author(s):  
M.N. Shkolnikova ◽  
◽  
E.A. Kadritskaya ◽  

The aim of the work was to substantiate the use of buckwheat husk for obtaining a functional food colorant melanin. The objects of the study were samples of buckwheat husk Fagopýrum esculéntum and isolated water-soluble melanin. The isolation of melanin was carried out by extraction of buckwheat husk with 4% NaOH at a hydraulic modulus of 1:20. The sorption capacity of the melanin extract was determined by its interaction with copper salt solutions in an alkaline medium. It has been established that a distinctive feature of buckwheat hulls is its low ash content, a rather high fiber content – 24.7–26.5%, and a sufficient content of pectin substances – 7.2–7.7% and melanin – 15.7–16.2%. The husk can be used for further processing in order to obtain the food coloring melanin. The experiment proved the ability of a melanin sample to bind copper ions: one gram of a 0.5% solution of buckwheat husk melanin extract bounded 966.6 mg/ml copper, and the antioxidant activity of one gram of melanin extract corresponds to the antioxidant activity of 0.056 g of rutin. Thus, water-soluble samples of melanin isolated from buckwheat hulls can be used as food coloring agents, as well as antioxidants and biosorbents, which makes it possible to recommend them as functional food ingredients in food.


2021 ◽  
Vol 65 (1) ◽  
pp. 109-122
Author(s):  
Enrique Sauri-Duch ◽  
Cesia Gutiérrez-Canul ◽  
Luis F. Cuevas-Glory ◽  
Lorena Ramón-Canul ◽  
Emilio Pérez-Pacheco ◽  
...  

Abstract The objective of this work was to investigate the variability of physicochemical parameters, phenolic compounds and in vitro antioxidant activity of propolis collected from different apiaries in southeastern Mexico. A high variability was found in the moisture content (1.96–8.26%), ash (0.66–5.50%) and sensory characteristics of raw propolis from southeastern Mexico, but the raw propolis samples met the requirements of the quality regulations. In the same way, most of the ethanolic extracts also complied with the quality regulations. Of all the extracts, PE2 obtained from Santa Cruz showed the highest values for dry extract, content of total phenolic compounds (TPC), total flavonoids (TF) and antioxidant activity (DPPH and ABTS). The content of the individual phenolic compounds varied according to the geographical location of the apiary, but the PE2 extract resulted in the highest pinocembrin and chrysin content. A positive correlation was obtained between TPC and TF with antioxidant activity. Propolis extracts were classified into two groups through principal component analysis (PCA). These results indicate that the apiary location in southeastern Mexico influenced the characteristics of propolis.


2021 ◽  
Vol 11 (2) ◽  
pp. 147
Author(s):  
Siska Esperanza Sinulingga ◽  
Loraetta Brety Sebayang ◽  
Samuel Sihotang

(Article History: Received August 27, 2021; Revised Sept 17, 2021; Accepted Sept 26, 2021) ABSTRAKInovasi bahan dasar yang dapat dijadikan teh herbal adalah jantung pisang, karena memiliki metabolit sekunder berupa flavonoid, kumarin dan golongan fenolik. Penelitian ini bertujuan untuk membuat teh herbal jantung pisang dan mengetahui standar mutunya. Pembuatan teh herbal dilakukan dengan variasi suhu pengeringan 50°C; 90°C; 110°C dan waktu pengeringan 110; 130; 150 menit kemudian dilakukan uji standar mutu dan skrining serbuk teh herbal. Hasil uji persyaratan mutu teh herbal:  kadar air (7,1%; 6,8%; 2,3%), kadar ekstrak dalam air (5,96%; 8,6%; 5 0,76%), kadar abu total (7,4%; 6,2%; 3,9%), kadar abu larut air (4,62%; 7,88%; 10,09%), kadar abu tidak larut asam (0,93%; 0,7%; 0,47%), kandungan flavonoid total 0,0002543 mg QE/g ekstrak dan cemaran mikroba 3,3×104 koloni/ml. Pembuatan teh jantung pisang dengan tambahan pemanis alami stevia memenuhi standar mutu produk teh herbal.Kata kunci: Teh herbal; jantung pisang; daun stevia; variasi suhu  ABSTRACTBasic ingredient innovation that can be used as herbal tea is banana blossom, because of its secondary metabolites such flavonoids, coumarins and phenolic groups. This study aims to make banana blossom herbal tea and determine the quality standard. Formulation of herbal tea by varying drying temperature of 50°C; 90°C; 110°C and drying time 110; 130; 150 minutes, the quality standard test and herbal tea powder screening were carried out. Results of herbal tea quality: water content (7.1%; 6.8%; 2.3%), extract content in water (5.96%; 8.6%; 5 0.76%), ash content total (7.4%; 6.2%; 3.9%) water soluble ash content (4.62%; 7.88%; 10.09%) acid insoluble ash content (0.93%; 0, 7%, 0.47%, total flavonoid content 0.0002543mg QE/g extract, microbial contamination 3.3×104 colonies/ml. Formulation of banana blossom tea with the addition of natural sweetener stevia meets the quality standards of herbal tea products,Keywords: Herbal tea; banana blossom; stevia leaf; temperature variation


Jurnal Kimia ◽  
2020 ◽  
pp. 37
Author(s):  
P. O. Samirana ◽  
N. W. Satriani ◽  
P. R. Harfa ◽  
S. P. P. Dewi ◽  
C. I. S. Arisanti

Aloe vera (Aloe vera) is a plant that is empirically often used to heal burns. Aloe vera leaf meat water extract contains saponins and flavonoids, in addition it also contains tannins and polyphenols. This research was conducted to determine whether the extracted water of Aloe vera leaf meat had met the parameters of extract quality standards so that it could be used in formulations. The steps taken are the extraction of aloe vera leaf meat with the method of infundation maceration, standardization of Aloe vera meat water extraction including testing the determination of drying shrinkage, total ash content, determination of ash content which is insoluble in acid, determination of the essence of water soluble extract, determination of the extract soluble in ethanol and chemical screening, identification with FT-IR, preparation of cream preparations, evaluation of cream preparations. Aloe vera leaf meat extract was obtained by infudation technique. Tests for drying drying extract produced 26.33%, total ash content of 1.3%, water soluble extract content of 11.9% and ethanol soluble extract content of 12.01%, total flavonoid content of 2.9%.   Keywords: Aloe Vera, Formulation, Cream, Burns.


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