PLASMA TRACE ELEMENT STATUS IN BETA-THALASSEMIA TRAIT PATIENTS

Objective: The study aims to evaluate the plasma trace element status in beta-thalassemia trait (BTT) patients and demonstrate the correlation between trace elements and hemoglobin (Hb)-A1, HbA2, and HbF.Methods: The study population consisted of 20 normal individuals and 40 patients with BTT aged between 25 and 55 years of both the sex. Hemoglobin variant analysis was performed in blood samples using cation exchange high-performance liquid chromatography. Patients were labeled as beta-thalassemia trait only if they had HbA2 >3.5% or HbF >2.0%. Plasma zinc was estimated by atomic absorption spectrometry, copper by bathocuproine disulfonate method, and iron by ferrozine method spectrophotometrically. Data were analyzed statistically by Student’s t -test and Pearson’s correlation using software SPSS version 20. p<0.05 was considered statistically significant.Results: Plasma zinc and copper decreased significantly in BTT patients compared to healthy controls. There was an apparent decrease in plasma iron in these patients. Degree of decline in zinc (p<0.001) was much greater than that of copper (p<0.04). Moreover, there was significant elevation of copper-zinc ratio and iron-zinc ratio (p<0.001) in BTT patients compared to controls. Both HbA1 and HbA2 correlated positively with both copper and iron. Interestingly, HbF showed negative correlation with all the three trace elements in BTT patients.Conclusion: The study highlights marked deficiency of plasma trace elements, hence, the need for periodic assessment and prompt administration of these micronutrients to reduce the complications in BTT patients. Further, ratios are more valuable markers in the determination of trace element status than individual elements.

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PLASMA TRACE ELEMENT STATUS IN BETA-THALASSEMIA TRAIT PATIENTS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v12i1.28922 ◽

2019 ◽

Vol 12 (1) ◽

pp. 232

Author(s):

Prabhakaran N ◽

Sudha K ◽

Reshma K ◽

Durgarao Y

Keyword(s):

Trace Elements ◽

Trace Element ◽

High Performance ◽

Absorption Spectrometry ◽

Beta Thalassemia ◽

Plasma Zinc ◽

Trace Element Status ◽

Apparent Decrease ◽

Thalassemia Trait ◽

Student’S T

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Atomic absorption spectrometry in wine analysis

Macedonian Journal of Chemistry and Chemical Engineering ◽

10.20450/mjcce.2009.218 ◽

2009 ◽

Vol 28 (1) ◽

pp. 17 ◽

Cited By ~ 22

Author(s):

Trajče Stafilov ◽

Irina Karadjova

Keyword(s):

Trace Elements ◽

Atomic Absorption Spectrometry ◽

Trace Element ◽

Atomic Absorption ◽

Absorption Spectrometry ◽

Analytical Control ◽

Allowable Limit ◽

Wine Production ◽

Element Determination ◽

Separation And Preconcentration

This article reviews methods for the determination and identification of trace elements in wine by using atomic absorption spectrometry (AAS). Wine is one of the most widely consumed beverages and strict analytical control of trace elements content is required during the whole process of wine production from grape to the final product. Levels of trace elements in wine are important from both points of view: organoleptic – Fe, Cu, Mn and Zn concentrations are directly related to the destabilization and oxidative evolution of wines, and toxicological – toxic elements content should be under the allowable limit, wine identification. The identification of metals in wine is subject of increasing interest since complexation may reduce their toxicity and bioavailability. AAS is one of widely used methods for routine analytical control of wine quality recommended by the International Organization of Vine and Wine. Two main approaches – preliminary sample digestion and direct instrumental measurement combined with AAS for trace element determination in wines are reviewed and discussed. Procedures for various sample pretreatments, for trace element separation and preconcentration are presented. Advances in metal identification studies in wines based on AAS are presented.

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Trace element nutrition in the tropical zebu cattle type. Lessons from the Gilgel Gibe catchment, Ethiopia

Afrika Focus ◽

10.21825/af.v27i1.4916 ◽

2014 ◽

Vol 27 (1) ◽

Author(s):

Veronique Dermauw

Keyword(s):

Trace Elements ◽

Trace Element ◽

Bos Indicus ◽

Zebu Cattle ◽

Health Production ◽

Animal Products ◽

Trace Element Status ◽

Trace Element Nutrition ◽

The Tropics ◽

The Impact

In the tropics, cattle are majorly dependent on natural pastures growing on poor soils and as a result prone to trace element deficiencies. This work investigated several aspects of trace element nutrition in zebu (Bos indicus) cattle, the most widely used cattle type in the tropics. Through several trials conducted at the Gilgel Gibe catchment, Ethiopia, we aimed to study factors influencing the flow of trace elements from soil to plant and grazing zebu cattle, and to investigate the impact of trace element status on health, production and trace elements in animal products. Overall, trace element, e.g., Cu, deficiencies were widely prevalent. Environment and certain grazing strategies were intimately related and affected trace element supply whereas cattle type affected trace element status. The effect of trace element status on trace elements in animal products was obvious, whereas the impact on health and production was less clear-cut. Key words: trace elements, minerals, cattle, zebu, Bos indicus, Ethiopia, tropics

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Concentration of Zinc, Copper, Selenium, Manganese, and Cu/Zn Ratio in Hair of Children and Adolescents with Myopia

Journal of Ophthalmology ◽

10.1155/2019/5643848 ◽

2019 ◽

Vol 2019 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Monika Fedor ◽

Beata Urban ◽

Katarzyna Socha ◽

Jolanta Soroczyńska ◽

Małgorzata Krętowska ◽

...

Keyword(s):

Trace Elements ◽

Children And Adolescents ◽

Trace Element ◽

Scalp Hair ◽

Absorption Spectrometry ◽

Zinc Level ◽

Control Group ◽

Hair Samples ◽

Insignificant Difference ◽

Eye Examination

The aim of this work was to determine the concentration of trace elements, zinc, copper, selenium, manganese, and Cu/Zn ratio, in scalp hair samples of children and adolescents with myopia. The study included 92 children (mean age 14.5 ± 2.5 years) with myopia and 43 healthy persons (mean age 11.8 ± 4.7 years). Each patient had a complete eye examination. Trace element concentrations in hair were determined by atomic absorption spectrometry. Cu/Zn ratio was also calculated. The zinc level in the hair of myopic patients was significantly higher (260 µg/g) in comparison to the control group (130 µg/g). There was a significantly lower Cu/Zn ratio in myopic patients (0.045) compared with controls (0.07). An insignificant difference was observed in the hair level of copper, selenium, and manganese between patients and controls. The results show that trace elements may play a significant role in the pathogenesis of myopia. Further studies should pay more attention to determine the effect of trace element on children myopia.

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THALASSEMIA SYNDROME AND OTHER HEMOGLOBINOPATHIES: A SINGLE THALASSEMIA CENTRE EXPERIENCE FROM TERTIARY CARE HOSPITAL OF PURULIA

10.36106/ijsr/1608252 ◽

2021 ◽

pp. 23-25

Author(s):

Sudipta Bera ◽

Tapobrata Biswas ◽

Anuradha Sinha ◽

Anindya Adhikari

Keyword(s):

High Performance ◽

Tertiary Care Hospital ◽

Sickle Cell Trait ◽

Tertiary Care ◽

Clinical History ◽

Thalassemia Major ◽

Beta Thalassemia ◽

Care Hospital ◽

Increased Risk ◽

Thalassemia Trait

Background: Hemoglobinopathies are one of the major health burden globally including India and Southeast Asian region. This study was carried out to nd out the prevalence of β-thalassemia, HbS, HbD, HbE as well as identication of asymptomatic carriers who have an increased risk of having a child with thalassemia in the western part of West Bengal. Materials and methods: In this retrospective cohort study, the data was collected from all newly registered patients with anemia, referred from different outdoor patients in a tertiary care hospital, attending thalassemia clinic over a period of 3 years from January2018 to December2020. Detailed clinical history was taken and blood samples collected are tested with Sysmex automated blood cell counter for red cell indices. Diagnosis of hemoglobinopathy was done by G8 HPLC (high performance liquid chromatography) Analyzer by TOSOH Bioscience. Results: A total 2297 cases were studied during the study period. The age of the patients ranged between 6 months to 60 years. Higher percentage of the study sample was at age of more than 10 years (93.8%). Among 2297 cases, 477 cases showed abnormal Hb fractions on HPLC. The following Hb abnormalities detected were following: β (beta) thalassemia trait 13.2%, β thalassemia major 2.3% followed by sickle cell trait 2.17%, HbE trait 1.8%, HbS disease 0.21%, Hb E disease 0.13%, hereditary persistence of fetal hemoglobin (HPFH) traits 0.17% and Hb D trait 0.08% along with HbE-β-thalassemia 0.30% and HbS-β-thalassemia 0.26%. Conclusion: Among the hemoglobinopathies, β-thalassemia trait (12.9%) is prevalent in western part of Bengal.

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Trace element nutrition in the tropical zebu cattle type. Lessons from the Gilgel Gibe catchment, Ethiopia

Afrika Focus ◽

10.1163/2031356x-02701006 ◽

2014 ◽

Vol 27 (1) ◽

pp. 87-95

Author(s):

Veronique Dermauw

Keyword(s):

Trace Elements ◽

Trace Element ◽

Bos Indicus ◽

Zebu Cattle ◽

Health Production ◽

Animal Products ◽

Clear Cut ◽

Trace Element Status ◽

The Tropics ◽

The Impact

In the tropics, cattle are majorly dependent on natural pastures growing on poor soils and as a result prone to trace element deficiencies. This work investigated several aspects of trace element nutri¬tion in zebu (Bos indicus) cattle, the most widely used cattle type in the tropics. Through several trials conducted at the Gilgel Gibe catchment, Ethiopia, we aimed to study factors influencing the flow of trace elements from soil to plant and grazing zebu cattle, and to investigate the impact of trace element status on health, production and trace elements in animal products. Overall, trace element, e.g., Cu, deficiencies were widely prevalent. Environment and certain grazing strategies were inti-mately related and affected trace element supply whereas cattle type affected trace element status. The effect of trace element status on trace elements in animal products was obvious, whereas the impact on health and production was less clear-cut.

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“PREVALENCE OF BETA-THALASSEMIA TRAIT IN PREGNANT WOMEN ATTENDING ANTENATAL CLINIC”

10.36106/paripex/4706167 ◽

2021 ◽

pp. 45-47

Author(s):

Uma Jain ◽

Preeti Gupta ◽

Shaily Sengar

Keyword(s):

Iron Deficiency ◽

Pregnant Women ◽

Iron Deficiency Anemia ◽

Gestational Age ◽

High Performance ◽

Hypochromic Anemia ◽

Antenatal Clinic ◽

Deficiency Anemia ◽

Beta Thalassemia ◽

Thalassemia Trait

INTRODUCTION- Iron deficiency anemia and thalassemia syndromes,especially beta thalassemia trait (BTT),are the two most commonly ccuring microcytic hypochromic anemias highly prevalent in countries like India. Iron deficiency anemia is the first most common cause of anemia in pregnancy,beta thalassemia trait is the second most frequent cause of anemia of pregnancy. Patients with thalassemia trait shows an increased incidence of anemia during the second trimester of pregnancy. Beta Thalassemia Trait (BTT) patients are usually asymptomatic and ignorant of their carrier state unless diagnosed by testing. Screening for thalassemia can be done by measuring HbA2 levels MATERIAL AND METHOD- This is a retrospective study. The data were collected from a clinic and associated st pathology in Shivpuri and a private maternity hospital in Gwalior from 1 January 2019 to 30 June 2021.94 patients were included in the study.All pregnant women between the age of 18-45 years and at any gestational age with hemoglobin level <10g/dL and microcytic hypochromic anemia (report of CBC) were included in the study. High-Performance Liquid Chromatography (HPLC) and serum ferritin report were collected. RESULTS- In our study the most common age group in which the anemia was found between 19-35 years (80.85%).Most of the patients were primigravida (44.68%),between 26-37 wks of gestational age (82.97%),from a rural area (70.21%). Only 2 patients had a family history (2.12%) of beta-thalassemia and 6 patients had a history (6.38%) of blood transfusion. In our study moderate anemia was found in 55.31% of cases followed by mild anemia 25.53% and severe anemia 19.14%. In our study iron deficiency anemia was found in 87.23% only minor Beta-thalassemia was found to be 4.25%, of the patient,and the coexistence of iron deficiency anemia and beta-thalassemia in pregnant women is 8.51%. In our study total of 12 patients (4 cases of only minor beta-thalassemia and 8 cases of minor beta-thalassemia associated with IDA) The prevalence rate of beta-thalassemia in our study was 7.8%. CONCLUSION- the recommendation is to diagnose beta-thalassemia in pregnant patients is essential to give proper treatment.The screening of pregnant patients prevents unnecessary parental iron therapy and iron overload in case of anemia.further studies are needed in this field and less expensive and accurate methods should come in place.

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Autism Spectrum Disorders: The Association with Inherited Metabolic Disorders and Some Trace Elements. A Retrospective Study

CNS & Neurological Disorders - Drug Targets ◽

10.2174/1871527318666190430162724 ◽

2019 ◽

Vol 18 (5) ◽

pp. 413-420 ◽

Cited By ~ 3

Author(s):

Wafaa Moustafa M. Abo El Fotoh ◽

Sameh Abdallah Abd El Naby ◽

Nahla M. Said Abd El Hady

Keyword(s):

Trace Elements ◽

Autism Spectrum Disorders ◽

Trace Element ◽

Metabolic Disorders ◽

Autism Spectrum ◽

High Serum ◽

Plasma Zinc ◽

Children With Asd ◽

Inherited Metabolic Disorders ◽

Spectrum Disorders

Background: Autism Spectrum Disorders (ASD) as a considerable health obstacle in kids is characterized by compromised social collaboration and stereotyped behavior. Autism is triggered by an interactive impact of environmental and genetic influences. Presumably, some inborn errors of metabolism are implicated in a sector of developmental disabilities. Also, several trace elements may have an important role in human behavior and neurological development. This study was designed to verify the frequency of inherited metabolic disorders and/or trace element abnormalities in children with ASD. Methods: In a retrospective analytical study, 320 children diagnosed with ASD according to the DSM-V criteria and Childhood Autism Rating Scale criteria were enrolled in this study. Serum ammonia, blood lactate, and arterial blood gases, plasma amino acid profile by tandem mass spectrophotometry, and a urinary organic acid assay were performed in all the patients. Likewise, the estimation of a number of trace elements in the form of serum lead, mercury, copper, and plasma zinc was done in all the patients. Results: A total of 320 children with ASD, inherited metabolic disorders were identified in eight (2.5%) patients as follows: seven (2.19%) patients with phenylketonuria, and one (0.31%) patient with glutaric aciduria type 1. Regarding the trace element deficiency, sixteen (5%) patients presented low plasma zinc level, five (1.56%) children presented a high serum copper level, two (0.62%) children presented a high serum lead level and only one (0.31%) autistic child presented high serum mercury level. Electroencephalogram (EEG) abnormalities were reported in 13.12% and Magnetic Resonant Imaging (MRI) abnormalities in 8.43% of cases. Conclusion: Screening for metabolic diseases and trace elements is required in all children diagnosed with ASD irrespective of any apparent clinical attributes of metabolic complaints and trace elements discrepancies.

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Trace elements in soils and crops

Philosophical Transactions of the Royal Society of London Series B Biological Sciences ◽

10.1098/rstb.1979.0087 ◽

1979 ◽

Vol 288 (1026) ◽

pp. 15-24 ◽

Cited By ~ 28

Keyword(s):

Trace Elements ◽

Trace Element ◽

Essential Elements ◽

Parent Material ◽

Soil Parameters ◽

Surface Soils ◽

Plant Parts ◽

Trace Element Status ◽

Element Mobilization ◽

Soil Surveys

To demonstrate the total amounts to be expected in soils, the ranges of contents of some 60 trace elements in ten representative Scottish arable surface soils are compared with ranges in soil-forming rocks and with crustal averages. It is, however, the amounts potentially available to plants rather than the total contents that are biologically significant. In temperate climates, trace element mobilization is greatest when weathering takes place under conditions of impeded pedological drainage, leading to the formation of gleyed soils. Mobilized trace elements occur in arable surface soils largely in adsorbed and chelated forms, which are available to plants to a greater or smaller extent depending on the prevailing soil parameters and on the element in question. Different species take up different amounts of trace elements: the proportions in the various plant parts vary with the element and the stage of growth. Information is required about the mobilization and uptake of many elements about which little is at present known but which may affect the functions of essential elements through inter-element interactions. Systematic soil surveys in which soils are mapped by associations related to parent material, with their series related to genetic soil types, provide a useful countrywide guide to trace element status.

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Blood and hair as non-invasive trace element biological indicators in growing rabbits

World Rabbit Science ◽

10.4995/wrs.2019.10654 ◽

2019 ◽

Vol 27 (1) ◽

pp. 21

Author(s):

George Papadomichelakis ◽

Athanasios C Pappas ◽

Evangelos Zoidis ◽

Georgios Danezis ◽

Konstantinos A Georgiou ◽

...

Keyword(s):

Trace Elements ◽

Trace Element ◽

Biological Indicators ◽

Organic Selenium ◽

Non Invasive ◽

Trace Element Status ◽

Element Profile ◽

Trace Element Contents ◽

Highly Correlated ◽

Toxic Trace Element

The suitability of blood and hair as non-invasive tools to monitor trace element contents was studied in 48 Hyla male growing rabbits. Three diets with increasing organic selenium (Se) addition (0.1, 0.5 and 2.5 mg/kg) were used to induce alterations in the concentrations of trace elements vs. an unsupplemented diet. In blood, a linear decrease in Co (P<0.001), Cu (P<0.001), Mn (P<0.05), Zn (P<0.05), Sb (P<0.001), As (P<0.001), Cr (P<0.001), Mo (P<0.001), Ni (P<0.001) and Cd (P<0.001) concentrations with increasing dietary Se was observed. In hair, a cubic effect of dietary Se on Co (P<0.01), Cu (P<0.05), Mn (P<0.001), Pb (P<0.05), Mo (P<0.05) and Cd (P<0.05) concentrations was found, while As, Cr and Ni concentrations decreased linearly (P<0.01, P<0.01 and P<0.001, respectively) with increasing dietary Se. Selenium was negatively correlated to Sb, As, Cr, Mo, Ni and Cd, (P<0.001) in blood, and to As (P<0.05), Cr, Ni (P<0.01) and Pb (P<0.05) in hair. The contents of Se, As, Cr and Ni in blood were highly correlated (P<0.001) to those in hair. Blood appeared to be more sensitive than hair in detecting small changes in the trace element profile in rabbits, as was indicated by the discriminant analysis. In conclusion, blood and hair can be suitable biological indicators of essential, toxic and potentially toxic trace element status in rabbits, particularly when used complementarily.

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