scholarly journals ENIGMATIC INDUCTION OF CYTOMIXIS IN ALLIUM CEPA ROOT MERISTEM BY AGLAIA EDULIS ROXB. LEAF EXTRACT AND ITS PHYTOCHEMICAL RATIONALE

2020 ◽  
pp. 168-171
Author(s):  
ARCHANA ELAMKULAM RAVINDRAN ◽  
JOHN ERNEST THOPPIL
Keyword(s):  
Allium Cepa ◽  
Leaf Extract ◽  
Root Meristem ◽  
Methanolic Extract ◽  
Synergistic Action ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Spectrometry Analysis ◽  
Dose Dependent

Objective: The present study aims to analyze the potential of Aglaia edulis Roxb. leaf extract to induce cytological aberrations in Allium cepa root meristem and to determine the phytoconstituents in the extract. Methods: Cytotoxicity evaluation of the leaf methanolic extract was done using Allium cepa assay using various concentrations. Volatile phytoconstituents in the extract were determined using gas chromatography–mass spectrometry analysis. Results: Considerable number of cytomictic cells along with other aberrations was observed. The occurrence of cytomixis was found to be dose dependent where it ranged from 6.58±0.35 to 29.45±0.45. The percentage of cytomictic cells among the total aberrant cells was observed between 35.19±1.67 and 77.39±1.39. The phytochemical analysis of the plant extract revealed the presence of active secondary metabolites. Conclusion: The synergistic action of the active compounds might have triggered the phenomenon of cytomixis which, in turn, could be exploited for the production of polyploids.

scholarly journals GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF METHANOLIC LEAF EXTRACT OF CASSIA ANGUSTIFOLIA VAHL.

2016 ◽  
Vol 9 (9) ◽  
pp. 111 ◽  
Author(s):  
Shahina Parveen ◽  
Anwar Shahzad ◽  
Anamica Upadhyay ◽  
Vikas Yadav
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Vitamin E ◽  
Leaf Extract ◽  
Methanolic Extract ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Spectrometry Analysis ◽  
Cassia Angustifolia ◽  
Chromatography Mass Spectrometry

ABSTRACTObjective: The leaves of Cassia angustifolia Vahl. are employed for the treatment of several human diseases. Therefore, the present study wasundertaken to determine the phytocomponents present in the methanolic extract of the leaves by gas chromatography-mass spectrometry (GC-MS)Methods: The collected leaf samples were dried and extracted in methanol. Screening of the extract was done by GC-MS which is an importanttechnique for the separation and identification of different phytochemicals.Results: The methanolic extract of the leaves of C. angustifolia revealed the presence of 45 different phytochemicals. The prevailing compounds were1 butanol, 3 methyl acetate (area % 7.47), 6, 6-dideutero-nonen-1-Ol-3 (area % 10.45), pentadecanoic acid (area % 9.22), and squalene (area %12.30). Vitamin E (area % 3.85) has also been found in the leaf extract. Some of the compounds possess biological activities.Conclusions: It can be concluded from the present study that some of the identified phytochemicals could be responsible for the medicinal value orbiological activity of the plant leaves.Keywords: Senna, Leaf extract, Methanol, Gas chromatography-mass spectrometry, Phytochemicals, Vitamin E.

scholarly journals MOSQUITO LARVICIDAL EFFICACY OF THE ACETONE LEAF EXTRACT OF SOLANUM TRILOBATUM AGAINST CULEX QUINQUEFASCIATUS AND AEDES AEGYPTI

2018 ◽  
Vol 11 (12) ◽  
pp. 273 ◽  
Author(s):  
Lalitha A ◽  
Thangapandiyan S
Keyword(s):  
Aedes Aegypti ◽  
Culex Quinquefasciatus ◽  
Leaf Extract ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Time Interval ◽  
Phytochemical Analysis ◽  
Spectrometry Analysis ◽  
Solanum Trilobatum ◽  
Larvicidal Activities

Objective: The main objective of our study is to control the vector-borne disease. It is one among the major disease burden in developing countries. There are lots of researches carried out using the various plant extracts against larvicidal activities of the Culex quinquefasciatus and Aedes aegypti. The present investigation was aimed to investigate the phytochemical analysis and mosquito larvicidal activities of Solanum trilobatum in acetone extract against the second instar larvae of C. quinquefasciatus and A. aegypti. Methods: The leaf extract of S. trilobatum was subjected to phytochemical analysis and Gas Chromatography–Mass Spectrometry analysis. The mortality rates of the second instar larvae were recorded after 24, 48, 72, and 96 h of exposure. The lethal concentration (LC)50 and LC90 were determined followed by probit analysis.Results: The LC50 values for C. quinquefasciatus were found to be 265.69 ppm, 227.59 ppm, 212.42 ppm, and 189.47 ppm at various time intervals, and the LC90 values were 558.27 ppm, 504.92 ppm, 479.09 ppm, and 444.28 ppm. Similarly, LC50 values for A. aegypti were noticed at 301.09 ppm, 256.01 ppm, 209.75 ppm, and 167.44 ppm and the LC90 values were 582.34 ppm, 477.52 ppm, 419.40 ppm, and 371.84 ppm for the time interval of 24 h, 48 h, 72 h, and 96 h.Conclusion: The result of the current work revealed that the leaf extract of the S. trilobatum has the potential to be acted as an alternative for the controlling of the mosquitoes.

scholarly journals IDENTIFICATION OF BIOACTIVE COMPOUNDS BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF SYZYGIUM JAMBOS (L.) COLLECTED FROM WESTERN GHATS REGION COIMBATORE, TAMIL NADU

2016 ◽  
Vol 10 (1) ◽  
pp. 364 ◽  
Author(s):  
Devakumar Devakumar Joseph ◽  
Keerthana Veerasamy ◽  
Sudha Siva Singaram
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Carboxylic Acid ◽  
Bioactive Compounds ◽  
Leaf Extract ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Spectrometry Analysis ◽  
Syzygium Jambos ◽  
Chromatography Mass Spectrometry

ABSTRACTObjective: The aim of this study was to investigate the presence of bioactive compounds in the methanolic leaf extract of Syzygium jambos.Methods: Collected leaves were shade dried and made into fine powder, extracted with methanol, and the methanolic extract was prepared andanalyzed for the presence of bioactive compounds by gas chromatography-mass spectrometry (GC-MS). The mass spectrum of the chromatographywas matched with NIST and WILEY Libraries.Results: The GC-MS analysis revealed the presence of 45 active compounds in the extract. From the GC-MS investigation, 1-Deoxy-d-mannitol3-methyl-2-methylsulfanyl-5-nitro-6-pyridin-4-ylpyrimidin-4-one, 3-Pentadecylphenol, 2-biphenylene carboxylic acid, Quinoline-3-carboxylic acid,and Stigmast-5-en-3-ol are important phytoconstituents which have antipyretic and antiparasitic activities.Conclusion: The present investigation revealed preliminary information on phytocompounds presented in S. jambos leaf extract which is very usefulfor the human community.Keywords: Syzygium jambos, Gas chromatography-mass spectrometry analysis, 1-Deoxy-d-mannitol, Phytoconstituents, Methanolic leaf extract.

scholarly journals GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF VIBURNUM OPULUS (L) EXTRACT AND ITS TOXICITY STUDIES IN RATS

2017 ◽  
Vol 10 (6) ◽  
pp. 383 ◽  
Author(s):  
Abiodun Humphrey Adebayo ◽  
Aristotle Balade ◽  
Omolara Faith Yakubu
Keyword(s):  
Gas Chromatography ◽  
Leaf Extract ◽  
Density Lipoprotein ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Water Control ◽  
Viburnum Opulus ◽  
Spectrometry Analysis ◽  
Water Fractions ◽  
Biochemical Analyses

Objectives: This study was aimed at establishing the antimicrobial and phytochemical profiles of Viburnum opulus (L) as well as the safety potential of the extract in albino Wistar rats.Methods: Ethanol, n-hexane, ethyl acetate, butanol and water fractions were prepared for both phytochemical assessment using gas chromatography-mass spectrum analysis (GC-MS). Five groups of seven rats were used for the study. Group A received distilled water (control), while groups B to E were treated respectively with 250, 500, 1000 and 1500 mg/kg body weight of V. opulus extract by abdominal canulisation for 28 days. Blood samples were obtained for biochemical analyses and the liver tissues were further processed for histological studies.Results: The GC-MS spectra revealed the existence of various phytoconstituents such as neophytadiene, germaciene, caryophyllene among others. High density lipoprotein and albumin were significantly (p<0.05) elevated in animals administered with 500, 1000 and 1500 mg/kg bw of the leaf extract. Ethanol, butanol and water fractions of the leaf of V. opulus showed antimicrobial action against most of the organisms used in this study.Conclusion: The result indicates the V. opulus leaf extract contains a wild range of fatty acids and heterocyclic compounds with antimicrobial efficacy and no hepatic damage.

scholarly journals Investigation of the In-Vivo Cytotoxicity and the In Silico-Prediction of MDM2-p53 Inhibitor Potential of Euphorbia peplus Methanolic Extract in Rats

Toxins ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 642 ◽  
Author(s):  
Yasmina M. Abd-Elhakim ◽  
Mohamed Abdo Nassan ◽  
Gamal A. Salem ◽  
Abdelkarim Sasi ◽  
Adil Aldhahrani ◽  
...  
Keyword(s):  
In Silico ◽  
Methanolic Extract ◽  
P53 Protein ◽  
Underlying Mechanism ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
P53 Expression ◽  
Spectrometry Analysis ◽  
Euphorbia Peplus

This study explored the probable in vivo cardiac and renal toxicities together with in silico approaches for predicting the apoptogenic potential of Euphorbia peplus methanolic extract (EPME) in rats. Cardiac and renal injury biomarkers were estimated with histopathological and immunohistochemical evaluations of both kidney and heart. The probable underlying mechanism of E. peplus compounds to potentiate p53 activity is examined using Molecular Operating Environment (MOE) docking software and validated experimentally by immunohistochemical localization of p53 protein in the kidney and heart tissues. The gas chromatography/mass spectrometry analysis of E. peplus revealed the presence of nine different compounds dominated by di-(2-ethylhexyl) phthalate (DEHP). Significant elevations of troponin, creatine phosphokinase, creatine kinase–myocardium bound, lactate dehydrogenase, aspartate transaminase, alkaline phosphatase, urea, creatinine, and uric acid were evident in the EPME treated rats. The EPME treated rats showed strong renal and cardiac p53 expression and moderate cardiac TNF-α expression. Further, our in silico results predicted the higher affinity and good inhibition of DEHP, glyceryl linolenate, and lucenin 2 to the MDM2-p53 interface compared to the standard reference 15 a compound. Conclusively, EPME long-term exposure could adversely affect the cardiac and renal tissues probably due to their inflammatory and apoptotic activity. Moreover, the in silico study hypothesizes that EPME inhibits MDM2-mediated degradation of p53 suggesting possible anticancer potentials which confirmed experimental by strong p53 expression in renal and cardiac tissues.

scholarly journals PHYTOCHEMICAL STUDIES AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF SARCOSTEMMA BREVISTIGMA, WIGHT & ARN.

2017 ◽  
Vol 10 (3) ◽  
pp. 462 ◽  
Author(s):  
Dhivya S M ◽  
Kalaichelvi K
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethanolic Extract ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Spectrometry Analysis ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Sarcostemma Brevistigma

ABSTRACTObjective: To explore the phytochemical constituents present in Sarcostemma brevistigma using gas chromatography-mass spectrometry (GC-MS).Methods: Preliminary phytochemical analysis of secondary metabolites was made by following standard procedures. GC-MS analysis of the plantextracts was performed by using GC-MS equipped with a DB-35MS capillary standard non-polar column and gas chromatograph interfaced to a MassSelective Detector (MS-DSQ-II) with Xcalibur software.Results: The qualitative phytochemical analysis of this species exhibited the presence of alkaloids, glycosides, flavonoids, phenols, steroids, aminoacids, tannins, terpenoids, quinones, and coumarin. The GC-MS analysis revealed the presence of 24 compounds in the ethanolic extract of aerial partof Sarcostemma brevistigma. The most prevailing compound was 14,17 bis(dimethylaminoamino)[3.3]paracyclophan-5,8-diacetonitrile (14.86%).Conclusion: The study concludes that the species Sarcostemma brevistigma is a potential source for bioactive compounds such as esters, alkanes,alcohols, alkenes, amide, amine, phenol, ketone, and so forth. This study justifies the traditional usage of this species.Keywords: Gas chromatography-mass spectrometry, Sarcostemma brevistigma, Phytochemical compounds, Medicinal plants.

scholarly journals Antioxidant, Antimicrobial and Cytotoxic Activities of Alcoholic Leaves Extracts of Spatholobus parviflorus (Roxb.Ex Dc.) Kuntze

2018 ◽  
Vol 9 (07) ◽  
Author(s):  
Jesy E. J. ◽  
Beena Jose
Keyword(s):  
Mass Spectrometry ◽  
Total Phenolics ◽  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Radical Scavenger ◽  
Cytotoxic Activities ◽  
Phytochemical Analysis ◽  
Spectrometry Analysis

Objective: To investigate the total phenolics, antioxidant , antimicrobial and cytotoxic, activities of ethanolic and methanolic leaves extracts of Spatholobus parviflorus (Roxb.ex Dc.) kuntze. Methods: The current study was focussed on broad parameters namely total phenolics, phytochemical analysis, gas chromatography-mass spectrometry analysis and antioxidant properties in order to characterize the alcoholic extracts of Spatholobus parviflorus as apotential free radical quencher. Results: The phytochemical screening of alcoholic extracts of Spaholobus parviflorus showed the presence of various secondary metabolites. Spatholobs parviflorus was proved to be an effective radical scavenger in all antioxidant assays. The gas chromatographic- mass spectrometry analysis confirmed the presence of bioactive compounds in varying percentage. Conclusion: These results suggest that the ethanolic extract of Spaholobus parviflorus has potential of antioxidant,antibacterial and cytotoxic activity that support the ethnopharmacological uses of this plant.The remarkable activity showed by the plant extract could be attributed to the synergic effect of the active compounds present in it.

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