scholarly journals PHYSICOCHEMICAL PROPERTIES AND NUTRITION OF MORINGA OLEIFERA LAM. LEAF EXTRACT: A PRELIMINARY STUDY ON PREPARATION PHYTOSOMES AS HERBAL SUPPLEMENT FOR CHILDREN

2022 ◽  
pp. 281-287
Author(s):  
MUTIA SARI WARDANA ◽  
MAHDI JUFRI ◽  
ABDUL MUN’IM
Keyword(s):  
Amino Acids ◽  
Particle Size ◽  
Zeta Potential ◽  
Physicochemical Properties ◽  
Crude Protein ◽  
Leaf Extract ◽  
Precipitation Method ◽  
Herbal Supplement ◽  
Preliminary Study ◽  
Moringa Leaf Extract

Objective: The study aimed to evaluate the physicochemical properties and nutrition of Moringa leaf extract. In addition, the preliminary study for the preparation of Moringa leaf extract-loaded phytosomes for a supplement. Methods: Extraction of Moringa leaf made using microwave-assisted extraction, followed by evaluation of proximate analysis (water, total ash, acid-insoluble of ash contents, and residual n-hexane), phytochemical screening, and nutrition such as crude protein, amino acids, and minerals (iron, zinc, and calcium). The phytosomes were prepared by the anti-solvent precipitation method and assessed for the morphology, particle size, zeta potential, polydispersity index (PDI), entrapment efficiency (EE), and Fourier-transform infrared spectra. Results: The nutrition contents of crude protein, iron, zinc, and calcium were 19.61±0.07%, 3.47±0.00 mg/100g, 5.46±0.05 mg/100g, and 747.40±4.89 mg/100g, respectively. The amino acids with the highest concentrations were glutamic acid, phenylalanine, aspartic acid, alanine, and arginine in the extract. The best preparation using sonication 10 min by morphology was a spherical included particle size, PDI, zeta potential, and EE of arginine was 87.16±1.73 nm, 0.22±0.04, −23.07±0.76 mV, and 108.94±0.52%, respectively. Conclusion: These preliminary results provide evidence of the nutritional benefit of Moringa leaf extract-loaded phytosomes as a promising supplement to prevent stunting in children.

scholarly journals FABRICATION AND IN VITRO CHARACTERIZATION OF A NOVEL NANOSUSPENSION OF TELMISARTAN: A POORLY SOLUBLE DRUG PREPARED BY ANTISOLVENT PRECIPITATION TECHNIQUE USING 33 FACTORIAL DESIGN

2020 ◽  
pp. 286-294
Author(s):  
PRASANTA KUMAR MOHAPATRA ◽  
SIREESHA ◽  
VAIBHAV RATHORE ◽  
HARISH CHANDRA VERMA ◽  
BIBHUTI PRASAD RATH ◽  
...  
Keyword(s):  
Particle Size ◽  
Drug Release ◽  
Zeta Potential ◽  
Dissolution Rate ◽  
Release Kinetics ◽  
Precipitation Method ◽  
Lauryl Sulfate ◽  
Poorly Soluble ◽  
Poorly Soluble Drug

Objective: The motivation behind the current examination was to build the solvency and dissolution rate of an antihypertensive drug telmisartan by the planning of nanosuspension by precipitation method at the research facility scale. We researched the nanoparticle manufacture of telmisartan employing a 33 factorial experimental configuration considering the impacts of nanosuspension on the physical, morphological, and dissolution properties of telmisartan. Methods: To get ready, nanosuspension particles of an ineffectively dissolvable drug are moreover of a drug solution to the anti-solvent leads to abrupt supersaturation and precipitation the making of nanoparticles. The nanosuspension particles of a poorly soluble drug loaded with urea and surfactants (sodium lauryl sulfate (SLS), poloxamer 188, Tween 80) have been prepared by a precipitation method. The nanosuspension particles were characterized for particle size, zeta potential, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), in vitro drug release, and release kinetics. Results: The readily optimized batch nanosuspension particles evaluated and exhibited the particle size (750 nm), zeta potential (-24.33 mV), differential scanning calorimetry (DSC) drug exhibited a change in crystalline form to amorphous, in vitro dissolution (F12 was higher 95% within 5 min) and drug release kinetics. The formulation parameter of surfactant concentration is optimized. Conclusion: The formulation of the nanosuspension approach has been shown to substantial improvement in the dissolution rate, thereby enhancing the oral bioavailability with the future development of this technology.

scholarly journals Penetapan Total Flavonoid, Aktivitas Antioksidan dan Karakterisasi Nanopartikel Ekstrak Etanol Daun Kelor (Moringa pterygosperma Gaertn.)

2019 ◽  
Vol 11 (1) ◽  
pp. 49
Author(s):  
Tinta Julianawati ◽  
Hendy Hendarto ◽  
Widjiati Widjiati
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Herbal Medicine ◽  
Leaf Extract ◽  
Total Flavonoid ◽  
Peroxyl Radicals ◽  
Chemical Components ◽  
Dpph Method ◽  
Moringa Leaf Extract

Moringa pterygosperma is one of the plants in Indonesia that is often used in herbal medicine. Moringa leaves contain many phytochemical compounds, one of the compounds is flavonoids which function as antioxidants. Flavonoid compounds in vitro can inhibit lipid peroxidation by breaking the chain of peroxyl radicals. Moringa leaf benefits in the field of herbal medicine are formed with a variety of preparations. One of the preparations is in the form of extracts with the aim of attracting chemical components to natural materials The extraction process has a disadvantage because the level of solubility in water is still low. The nanoparticle technique is a strategy to increase the bioavalbicibility of herbal active compounds. This research was experimental in order to determine the total flavonoid levels contained in Moringa leaf extract. In addition, it was also to find out the antioxidant activity and particle size of the moringa leaf extract nanoparticles. Determination of flavonoid levels using spectrophotometric methods. The antioxidant activity of moringa leaf extract nanoparticles was tested using the DPPH method (2,2-diphenyl-picrylhdrazyl) and to determine the particle size used by using the Particle Size Analyzer (PSA). The results of the study showed total flavonoid levels in moringa leaf extract (1.97 ± 1.07)%. Antioxidant activity of 451.8 ppm with a reduction of 2.8% and particle size in the moringa leaf extract nanoparticles of ± 134.78 nm. Moringa leaf extract nanoparticles include low antioxidants. Keywords: moringa; nanoparticles; antioxidants

scholarly journals Preparation and Characterization of Betel Leaves (Piper betle Linn) Extract Nanoparticle with Ionic Gelation Method

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Dwi Saryanti ◽  
Dian Nugraheni ◽  
Nisa Sindi Astuti
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Leaf Extract ◽  
Particle Morphology ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
Piper Betle ◽  
Ionic Gelation ◽  
Betel Leaf

Nanoparticles are used in drug delivery which can increase mass transfer so increase the absorption and effectiveness of the drug. Therefore, its prospect to improve antibacterial and antioxidants activities of betel leaves. The research aimed to preparation and characterization of betel leaf extract using ionic gelation technique. The formulation of nanoparticles from betel leaf extract with ionic gelation method using alginate and CaCl2 with a ratio of 2.5: 1. The characterization of the nanoparticles includes particle size analysis, zeta potential, particle morphology and determination of flavonoid content. Particle size analysis demonstrated that the betel leaf extract nanoparticles had a particle size of 243,03 ± 1,48 nm, zeta potential of -23,0 ± 0,35 mV and morphology of particle showed that a flat shape. The betle leaf exctract nanoparticle positively contained flavonoid with Rf 0.7 equivalent to quercetin. The betel leaf extract can be made nanoparticles with ionic gelation method using alginate and CaCl2.

scholarly journals Effects of Gallic Acid by Ultrasound on Physicochemical Properties of Lateolabrax Japonicas Myofibrillar Protein

2021 ◽  
Vol 251 ◽  
pp. 02043
Author(s):  
Yingchang Li ◽  
Yuanyuan Li ◽  
Qiuying Li ◽  
Yanfang Lv ◽  
Shumin Yi
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Atpase Activity ◽  
Physicochemical Properties ◽  
Gallic Acid ◽  
Sulfhydryl Group ◽  
Myofibrillar Protein ◽  
Protein Solution ◽  
Solution System ◽  
Tyrosine Content

The effects of different concentrations of gallic acid (0 mg/g, 1 mg/g, 2 mg/g, 4 mg/g, and 6 mg/g) on the physicochemical properties of Lateolabrax japonicas myofibrillar protein were studied with 400 W ultrasound. The results showed that gallic acid decreased the particle size, total sulfhydryl group, carbonyl and dimer tyrosine content and Ca2+-ATPase activity (P<0.05) of myofibrillar protein, however, increased the zeta potential. Ultrasonic wave could cooperate with gallic acid to slow down protein oxidation and make the protein solution system more stable. When the concentration of gallic acid was 2 mg/g, the indicators of protein solution were most favorable, which improved the properties of protein.

scholarly journals Penetapan Total Flavonoid, Aktivitas Antioksidan dan Karakterisasi Nanopartikel Ekstrak Etanol Daun Kelor (Moringa pterygosperma Gaertn.)

2019 ◽  
Vol 11 (1) ◽  
pp. 49
Author(s):  
Tinta Julianawati ◽  
Hendy Hendarto ◽  
Widjiati Widjiati
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Herbal Medicine ◽  
Leaf Extract ◽  
Total Flavonoid ◽  
Peroxyl Radicals ◽  
Chemical Components ◽  
Dpph Method ◽  
Moringa Leaf Extract

Moringa pterygosperma is one of the plants in Indonesia that is often used in herbal medicine. Moringa leaves contain many phytochemical compounds, one of the compounds is flavonoids which function as antioxidants. Flavonoid compounds in vitro can inhibit lipid peroxidation by breaking the chain of peroxyl radicals. Moringa leaf benefits in the field of herbal medicine are formed with a variety of preparations. One of the preparations is in the form of extracts with the aim of attracting chemical components to natural materials The extraction process has a disadvantage because the level of solubility in water is still low. The nanoparticle technique is a strategy to increase the bioavalbicibility of herbal active compounds. This research was experimental in order to determine the total flavonoid levels contained in Moringa leaf extract. In addition, it was also to find out the antioxidant activity and particle size of the moringa leaf extract nanoparticles. Determination of flavonoid levels using spectrophotometric methods. The antioxidant activity of moringa leaf extract nanoparticles was tested using the DPPH method (2,2-diphenyl-picrylhdrazyl) and to determine the particle size used by using the Particle Size Analyzer (PSA). The results of the study showed total flavonoid levels in moringa leaf extract (1.97 ± 1.07)%. Antioxidant activity of 451.8 ppm with a reduction of 2.8% and particle size in the moringa leaf extract nanoparticles of ± 134.78 nm. Moringa leaf extract nanoparticles include low antioxidants. Keywords: moringa; nanoparticles; antioxidants

FORMULATION AND EVALUATION OF STABILIZED EPROSARTAN NANOSUSPENSION

2020 ◽  
pp. 83-87
Author(s):  
M. SANTHOSH RAJA ◽  
K. VENKATARAMANA
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Polymeric Nanoparticles ◽  
Precipitation Method ◽  
Dissolution Testing ◽  
Potential Drug ◽  
Dsc Analysis ◽  
Scanning Calorimetry ◽  
Bcs Class Ii ◽  
Pvp K30

Objective: The objective of the current study is to enhance the solubility of Eprosartan mesylate a BCS Class II drug by employing the nanoprecipitation technique. Methods: Polymeric nanoparticles of Eprosartan mesylate were prepared by precipitation technique with various polymers like PVP K30, HPMC K15M, and Eudragit L100 in various ratios. The incompatibility issues which may arise between the drug and polymers were tested by differential scanning calorimetry (DSC). The formed nanosuspensions were evaluated for various parameters like particle size, zeta potential, drug content, and dissolution testing. Results: Among all the nanosuspension formulations, E12 formulation prepared with Eudragit L 100 showed better evaluation characteristics. SEM and DSC analysis showed no major interactions with the excipients. The maximum drug release was showed at 12h. The formulation E12 showed the particle size of 81.5±5.5 nm and zeta potential of-55.1mv. Conclusion: The nano-precipitation method improved the dissolution as well as the bioavailability of Eprosartan mesylate nanosuspension.

Effect of Solid Lipid and Liquid Oil Ratios on Properties of Nanostructured Lipid Carriers for Oral Curcumin Delivery

2014 ◽  
Vol 1060 ◽  
pp. 62-65 ◽  
Author(s):  
Yaowaporn Sangsen ◽  
Punsupang Laochai ◽  
Pravara Chotsathidchai ◽  
Ruedeekorn Wiwattanapatapee
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Sustained Release ◽  
Physicochemical Properties ◽  
Nanostructured Lipid Carriers ◽  
Loading Capacity ◽  
High Shear ◽  
Solid Lipid ◽  
High Shear Homogenization ◽  
Xrd Techniques

In this study, three nanostructured lipid carriers (NLC) formulations comprised of varying ratios of lipid (Compritol® 888 ATO) and oil (Labrafac® CC) including 4:1, 3:2, and 2.5:2.5, were developed by high shear homogenization technique. The effect of different ratios on the physicochemical properties and release profiles of the formulations were investigated. Increasing the amount of liquid oil increased the particle size and zeta potential whereas decreased size distribution of the blank and curcumin loaded NLC. However, the entrapment efficacy and loading capacity of the NLC for curcumin were not increased following such ratios. The different ratios were not influence on the sequence of sustained release of curcumin from the NLC over 60 h. Moreover, the amorphous curcumin and crystalline behavior of the optimized NLC were characterized by DSC and XRD techniques. Thus, the effect of the proportions of solid lipid and liquid oil in the formulations should be considered for development of suitable NLC system for oral curcumin delivery.

scholarly journals Development of a Resveratrol Nanosuspension Using the Antisolvent Precipitation Method without Solvent Removal, Based on a Quality by Design (QbD) Approach

Pharmaceutics ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 688 ◽  
Author(s):  
Do-Hoon Kuk ◽  
Eun-Sol Ha ◽  
Dong-Hyun Ha ◽  
Woo-Yong Sim ◽  
Seon-Kwang Lee ◽  
...  
Keyword(s):  
Particle Size ◽  
Plasma Concentration ◽  
Zeta Potential ◽  
Oral Bioavailability ◽  
Quality By Design ◽  
Raw Material ◽  
Precipitation Method ◽  
Maximum Plasma ◽  
Lauryl Sulfate ◽  
Solvent Removal

The purpose of this study was to develop a resveratrol nanosuspension with enhanced oral bioavailability, based on an understanding of the formulation and process parameters of nanosuspensions and using a quality by design (QbD) approach. Particularly, the antisolvent method, which requires no solvent removal and no heating, is newly applied to prepare resveratrol nanosuspension. To ensure the quality of the resveratrol nanosuspensions, a quality target product profile (QTPP) was defined. The particle size (z-average, d90), zeta potential, and drug content parameters affecting the QTPP were selected as critical quality attributes (CQAs). The optimum composition obtained using a 3-factor, 3-level Box–Behnken design was as follows: polyvinylpyrrolidone vinyl acetate (10 mg/mL), polyvinylpyrrolidone K12 (5 mg/mL), sodium lauryl sulfate (1 mg/mL), and diethylene glycol monoethyl ether (DEGEE, 5% v/v) at a resveratrol concentration of 5 mg/mL. The initial particle size (z-average) was 46.3 nm and the zeta potential was −38.02 mV. The robustness of the antisolvent process using the optimized composition conditions was ensured by a full factorial design. The dissolution rate of the optimized resveratrol nanosuspension was significantly greater than that of the resveratrol raw material. An in vivo pharmacokinetic study in rats showed that the area under the plasma concentration versus time curve (AUC0–12h) and the maximum plasma concentration (Cmax) respectively, than those of the resveratrol raw material. Therefore, the prepara values of the resveratrol nanosuspension were approximately 1.6- and 5.7-fold higher,tion of a resveratrol nanosuspension using the QbD approach may be an effective strategy for the development of a new dosage form of resveratrol, with enhanced oral bioavailability.

scholarly journals EFFECTS OF GELATIN AND GLUTARALDEHYDE CONCENTRATIONS ON CHARACTERISTICS OF CANTIGI (Vaccinium Varingiaefolium Miq.) EXTRACT LOADED GELATIN NANOPARTICLES AS ANTIOXIDANT

2021 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Kosasih Kosasih ◽  
Wahono Sumaryono ◽  
Diky Mudhakir ◽  
Agus Supriyono ◽  
Yulius Evan Christian ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Zeta Potential ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Particle Sizes ◽  
Endemic Plant ◽  
Gelatin Nanoparticles ◽  
Nanoparticle Morphology

Cantigi is an endemic plant of sub-alpine area of Mount Tangkuban Parahu in Bandung, Indonesia. Previous study showed ethanol extract of young red leaves had antioxidant activity, however no information on this activity if changed into nanoparticles. The purpose of this study was to determine the effects of gelatin and glutaraldehyde concentrations on the characteristics of Cantigi extract loaded gelatin nanoparticles and to evaluate the antioxidant activity of nanoparticles. Cantigi leaves were extracted by maceration using n-hexane, ethyl acetate, and ethanol 96%. The ethanol extract was dried, made into nanoparticles by varying gelatin (0.1; 0.2; and 0.3 g) and glutaraldehyde (0.1; 0.2; and 0.3 mL) amounts, and conducted at 500 rpm and 40 °C for 3 hours. Nanoparticles were evaluated for particle size, zeta potential, morphology, and antioxidant activity. Nanoparticles with glutaraldehyde amount variation had particle sizes (PS) of 105.9±26.2; 37.1±8.7; and 32.5±7.4 nm; polydispersity indeces (PI) of 0.508; 0.717; and 0.563; zeta potential values (ZPV) of 0.55; 0.89; and 0.78 mV; and antioxidant activities (IC50) of 56.15±0.16; 53.67±0.10; and 51.57±0.39 ppm, respectively. Then, nanoparticles with gelatin amounts variation had PS of 22.5±5.1; 37.1±8.7; and 83.3±21 nm; PI of 0.604; 0.717; 0.326; ZPV of 1.27; 0.89; 0.18 mV; and antioxidant activities of 51.58±0.19; 53.67±0.12; and 55.46±0.04 ppm, respectively. Nanoparticle morphology was spherical. Cantigi leaf extract can be made into gelatin nanoparticles; the smaller the concentration of the polymer used and higher the concentration of the glutaraldehyde, the smaller the resulted particle size and increased antioxidant activity. Antioxidant activities of nanoparticles was lower than those of the extract (IC50 16.84±0.30 ppm).

scholarly journals OPTIMIZATION OF SODIUM DEOXYCHOLATE-BASED TRANSFERSOMES FOR PERCUTANEOUS DELIVERY OF PEPTIDES AND PROTEINS

2019 ◽  
pp. 329-332 ◽  
Author(s):  
ASTRIED LEONYZA ◽  
SILVIA SURINI
Keyword(s):  
Thin Film ◽  
Particle Size ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Zeta Potential ◽  
Sodium Deoxycholate ◽  
Polydispersity Index ◽  
Tem Analysis ◽  
Epidermal Growth ◽  
Preliminary Study

Objective: This study aims to formulate and characterize the transfersomes for percutaneous delivery of peptides and proteins. In particular, this study was a preliminary study for the transfersomes formulation of recombinant human epidermal growth factor (rhEGF) for topical delivery. Methods: The transfersomes was prepared by thin film hydration method using phosphatidylcholine and sodium deoxycholate as vesicle former. In this study, transfersomes formulas were optimized, namely TF1, TF2, TF3, and TF4 with several ratios of phospholipid and surfactant which were 90:10, 85:15, 80:20, and 75:25, respectively. Afterward, the transfersomes were characterized in terms of particle size distribution, polydispersity index, zeta potential, morphology of vesicles, and deformability index. Results: The results showed that the best formulation was TF3 with the ratio of 80:20 with a particle size of 118.6±1.33 nm, polydispersity index of 0.102±0.011, zeta potential of-30.9±0,46 mV, and deformability index of 1.182±0.08. TEM analysis also showed spherical and unilamellar vesicles of transfersomes. Conclusion: This work demonstrated that the sodium deoxycholate-based transfersomes could be potential to be further formulated with peptide and protein for percutaneous delivery.

Sign in / Sign up

Export Citation Format

Share Document