scholarly journals VARIATIONS IN PHYTOCONSTITUENTS AND ANTIMICROBIAL ACTIVITIES IN ECOTYPES OF OXALIS CORNICULATA L. AND OXALIS DEBILIS KUNTH

2016 ◽  
Vol 8 (10) ◽  
pp. 270
Author(s):  
Eleena Panda ◽  
Chinmay Pradhan ◽  
Anath Bandhu Das
Keyword(s):  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Leaf Extracts ◽  
The Family ◽  
Zones Of Inhibition ◽  
New Compounds ◽  
First Time ◽  
Better Than

<p><strong>Objective: </strong>The present study aims at the comparative analysis of phytoconstituents and antimicrobial activities of four ecotypes of Odisha, India, each of <em>Oxalis corniculata </em>L. (OC-Eco-1, OC-Eco-2, OC-Eco-3, OC-Eco-4) and <em>Oxalis debilis</em> Kunth. (OD-Eco-1, OD-Eco-2, OD-Eco-3, OD-Eco-4) of the family Oxalidaceae.</p><p><strong>Methods: </strong>The ecotypes were collected from four districts (Balasore, Bhadrak, Jajpur, Khurda) of Odisha, India. The qualitative phytochemical screening was done to test glycosides, saponins, tannins and terpenoids. Further analysis of phytochemicals was also screened through TLC and HPTLC. Antimicrobial potentiality of leaf extract of various ecotypes was studied against <em>Bacillus subtilis</em>, <em>Pseudomonas aeruginosa</em> and <em>Streptococcus epidermis</em> by disc diffusion method.</p><p><strong>Results: </strong>Tannin and terpenoids estimation revealed that significantly high amount of phytoconstituents were present in OD-Eco-3 of <em>O. debilis</em>. The methanolic leaf extracts of each ecotype were screened for qualitative analysis of phytochemical through TLC and HPTLC that showed maximum 16 compounds in <em>O. debilis </em>(OD-Eco-3)<em> </em>a new report, as compared to <em>O. corniculata. </em>All the four ecotypes of <em>O. debilis</em><em> </em>showed some new HPTLC bands (Rf 0.96, 0.90, 0.90, 0.81) as compared to <em>O. corniculata </em>that occupied 7.70%, 22.38%, 23.79% and 34.42% of peak area respectively. Cluster analysis on the basis of HPTLC banding pattern showed a close affinity among ecotypes of each species. Crude extracts showed antibacterial activity against <em>B. subtilis</em>, <em>P. aeruginosa</em> and <em>S. epidermis</em>. Leaf extracts of ecotypes of <em>O. corniculata</em> showed zones of inhibition and MIC better than that of <em>O. debilis </em>against <em>S. epidermis</em>.<strong></strong></p><p><strong>Conclusion: </strong>The findings significantly reported some new compounds for the first time in <em>O. debilis. </em>The study also indicated a promising potential of antimicrobial activity of <em>O. debilis</em> which was the first report.</p>

scholarly journals Synthesis and Evaluation of Antimicrobial Activities of New Piperidine Derivatives

2020 ◽  
Vol 10 (6) ◽  
pp. 7177-7186
Keyword(s):  
Nitrogen Heterocycles ◽  
Bacterial Pathogen ◽  
Structural Features ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Gram Negative ◽  
Wittig Olefination ◽  
New Compounds

Nitrogen heterocycles with piperidine rings are the most prominent structural features and frequently utilized by pharmaceuticals. In this study, we have disclosed the synthesis of new compounds with piperidine motif. The synthesis of these derivatives was achieved using Wittig olefination, O-alkylation, and nucleophilic substitution reaction. The antimicrobial activity was performed by disc diffusion method utilizing Staphylococcus aureus as gram-positive and Escherichia coli as a gram-negative bacterial pathogen, respectively.

scholarly journals Antibacterial Activity of Leaf Extracts ofBaeckea frutescensagainst Methicillin-ResistantStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Somayeh Razmavar ◽  
Mahmood Ameen Abdulla ◽  
Salmah Binti Ismail ◽  
Pouya Hassandarvish
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Bioactive Constituents ◽  
Disc Diffusion Method ◽  
Leaf Extracts ◽  
Baeckea Frutescens ◽  
High Degree

This study was based on screening antibacterial activity of the ethanol extract ofBaeckea frutescensL. against MRSA clinical isolates, analyzes the potential antibacterial compound, and assesses the cytotoxicity effect of the extract in tissue culture. Leaves ofBaeckea frutescensL. were shade dried, powdered, and extracted using solvent ethanol. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, steroids, terpenoids, phenols, and carbohydrates. The presence of these bioactive constituents is related to the antibacterial activity of the plant. Disc diffusion method revealed a high degree of activity against microorganisms. The results confirm thatBaeckea frutescensL. can be used as a source of drugs to fight infections caused by susceptible bacteria.

scholarly journals LC-ESI/MS and GC-MS Methanol Extract Analysis, Phytochemical and Antimicrobial Activity Studies of Centella asiatica

2020 ◽  
pp. 32-51
Author(s):  
Daniel A. Ondeko ◽  
Bernard F. Juma ◽  
Lilechi D. Baraza ◽  
Peter K. Nyongesa
Keyword(s):  
Chemical Constituents ◽  
Centella Asiatica ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Disc Diffusion Method ◽  
Leaf Extracts ◽  
Methanolic Extracts ◽  
Antibacterial And Antifungal

Aims: To determine chemical constituents of the Leaf extracts of Centella asiatica using the LC-MS and GC-MS and their antimicrobial activities. Study Design: Structural determination of compounds from the leaf extracts was done using GC-MS and LC-MS analysis. The antimicrobial properties of the extracts were done using disc diffusion method. Place and Duration of Study:  Pure and Applied Chemistry Department, Masinde Muliro University of Science and Technology, Kenya: Between 2016-2019. Methodology: Plant materials of C. asiatica were sequentially extracted separately based on the polarity viz., hexane, ethyl acetate and methanol. Determination of chemical constituents was done using LC-MS and GC-MS analysis and phytochemical screening. The extracts were assayed against Staphylococcus aureus, Klebsiella pneumonia, Escherichia coli and Candida albicans. Standard antimicrobials viz. ampicillin and Nystatin were used as the control. Disc diffusion method was used and zones of inhibition, after respective incubation periods, were used to quantify antibacterial and antifungal activity. Results: Phytochemical screening of the hexane, ethyl acetate and methanolic extracts showed that terpenoids, flavonoids, saponins, alkaloids, steroids, amino acids and proteins, reducing sugars and carbohydrates were present. LC-MS and GC-MS analyses of the methanolic extracts identified 22 and 33 compounds, respectively, by use of the national institute of standards and technology (NIST) library. The extracts showed appreciable activity against common microbes tested. Conclusion: This study forms the basis for the biological characterization and significance of the compounds identified in the leaf and stem extracts of C. asiatica. These compounds are known to possess antibacterial and antifungal activities that could be established as potential candidates for future drug development. However, these extracts, need to be subjected to further chromatographic procedures to isolate the identified compounds and their bioactivities determined.

scholarly journals IN-VITROSTUDIES ON ANTIFUNGALACTIVITY OF SOMEMISTLETOE SPECIES

2021 ◽  
Vol 9 (06) ◽  
pp. 420-427
Author(s):  
R. Neelima ◽  
◽  
B. Sujatha ◽  
N.V. Ramana ◽  
B.V. Rao ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Phytophthora Infestans ◽  
Inhibitory Activity ◽  
Sclerotium Rolfsii ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Leaf Extracts

In vitro evaluation of antifungal activity by disc diffusion method was carried out on leaf extracts of D.falcata, D. falcata var pubescens, V. monoicum and V. orientale and stem extracts of D. falcata, D. falcata var pubescens, V. articulatum and V.orientale using three different solvents viz., methanol, n-hexane and ethyl acetate. The antifungal activity was tested on three fungal strains include Fusarium oxysporum, Phytophthora infestans, Sclerotium rolfsii. Overall leaf extracts exerted better inhibitory activity than stem counterparts. Among all, only V. orientale was effective against the three fungal species.Compared to stem extracts, leaf extracts of D. falcata var pubescens have shown higher antifungal activity.

scholarly journals Biogenetic Conversion of Wyerone and Dihydrowyerone into Wyerone Epoxide in Vicia faba Cotyledons and Screening of Antibacterial Activity

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Nedhal A. Al-Douri ◽  
Randa N. Haddadin ◽  
Ashok K. Shakya ◽  
Ghaleb A. Oriquat
Keyword(s):  
Antibacterial Activity ◽  
Vicia Faba ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Stress Factors ◽  
Gram Positive Bacteria ◽  
The Family ◽  
Zones Of Inhibition ◽  
Plant Produce ◽  
First Time

Background. Vicia faba is a plant that belongs to the family Fabaceae. Cotyledons of this plant produce compounds called phytoalexins as a result of fungal and bacterial infection or stress factors. The phytoalexins are furanoacetylenic compounds. They include wyerone, wyerol, wyerone acid, their dihydro derivatives, and wyerone epoxide. These compounds have antimicrobial activities mainly against fungi. Objective. The purpose of this study was to elucidate the biogenetic conversion of wyerone and dihydrowyerone to wyerone epoxide in V. faba and to investigate the antibacterial activities of some of these phytoalexins. Materials and Methods. Seeds of Vicia faba were used. Labelled wyerone and dihydrowyerone were obtained by treating CuCl2-induced cotyledons with sodium (2-14C) acetate and separated by TLC and HPLC. Labelled wyerone and dihydrowyerone were then applied to induced bean cotyledons to establish any possible interconversion to wyerone epoxide. Antibacterial activity of wyerone, wyerone acid, and wyerone epoxide was investigated by disc diffusion test against a panel of microorganisms. Zones of inhibition were reported. Results. The radiolabeling studies showed that 12.4% of 14C-wyerone, and 6.01% of 14C-dihydrowyerone were incorporated into wyerone epoxide. This indicates that wyerone epoxide was most probably derived from wyerone and dihydrowyerone. In addition, a new compound, 11-hydroxywyerone was isolated for the first time. Additionally, this study showed that wyerone, wyerone epoxide, and wyerone acid had no antibacterial activity against Gram-negative bacteria but were active against Gram-positive bacteria.

scholarly journals Antimicrobial Activities of Essential Oil and Methanol Extract ofTeucrium montanum

2007 ◽  
Vol 4 (s1) ◽  
pp. 17-20 ◽  
Author(s):  
Nenad Vukovic ◽  
Tanja Milosevic ◽  
Slobodan Sukdolak ◽  
Slavica Solujic
Keyword(s):  
Essential Oil ◽  
Methanol Extract ◽  
Antimicrobial Activities ◽  
Fungal Species ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Inhibitory Effects ◽  
Disc Diffusion Method ◽  
Antifungal Effect

This study was designed to examine the chemical composition of essential oil and thein vitroantimicrobial activities of essential oil and methanol extract ofTeucrium montanum. The inhibitory effects of essential oil and methanol extracts ofT. montanumwere tested against 13 bacterial and three fungal species by using disc-diffusion method. GC/MS analyses revealed that essential oil contains mainly δ-cadinene (17.19%), β-selinene (8.16%) α-calacorene (4.97%), 1,6-dimethyl-4-(1-methylethyl)-naphthalene (4.91%), caryophyllene (4.35%), copaene (4.23%), torreyol (3.91%), 4-terpineol (3.90%), cadina-1,4-diene (3.39%), β-sesquiphellandrene (3.34%), τ-cadinol (3.12%) and γ-curcumene (3.18%). The essential oil has antibacterial as well as antifungal effect.

scholarly journals Antioxidant and antimicrobial effect of alkaloid bulbs extract of Polianthes tuberosa L. (Amaryllidaceae) cultivated in Algeria

2020 ◽  
Vol 10 (4) ◽  
pp. 44-48
Author(s):  
Nihed BARGHOUT ◽  
Nada CHEBATA ◽  
Saida MOUMENE ◽  
Seddik KHENNOUF ◽  
Abdelaziz GHARBI ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Scavenging Effect ◽  
Alkaloid Extracts

Polianthes tuberosa L. (Amaryllidaceae) is an ornamental and medicinal plant. Its flowers and bulbs are used traditionally as a diuretic, emetic, against rashes and gonorrhea. The aim of this work was, to evaluate the antioxidant and antimicrobial activities of bulbs and bulbils alkaloid extracts of P. tuberosa. Antiradical effect was assessed against DPPH radical. However, antimicrobial activity was measured through the disc diffusion method against Escherichae coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Staphylococcus aureus resistant to Methicillin (MRSA) and Candida albicans ATCC 90028. The scavenging effect against DPPH showed that the bulbs and bulbils alkaloids extracts exhibited an antiradical effect with IC50 = 0.231±0.017 mg/mL and 0.233±0.093 mg/mL respectively, less than the effect of vitamin C with IC50 = 0.0194±0.0002 mg/mL. Antimicrobial activity results reveal that both alkaloid bulbs extracts at 50 mg/mL did not have any inhibitory effect against the studied strains using the disc diffusion method. According to this work, bulbs and bulbils alkaloid extracts show a moderate antioxidant effect; that could be recommended as a natural antioxidant. Although tuberose bulbs were used traditionally as a soap substitute; bulbs alkaloid extract has no antimicrobial effect. Keywords: Polianthes tuberosa L., bulbs, bulbils, alkaloids, antiradical activity, antimicrobial activity.

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