scholarly journals Antioxidant and antimicrobial effect of alkaloid bulbs extract of Polianthes tuberosa L. (Amaryllidaceae) cultivated in Algeria

2020 ◽  
Vol 10 (4) ◽  
pp. 44-48
Author(s):  
Nihed BARGHOUT ◽  
Nada CHEBATA ◽  
Saida MOUMENE ◽  
Seddik KHENNOUF ◽  
Abdelaziz GHARBI ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Scavenging Effect ◽  
Alkaloid Extracts

Polianthes tuberosa L. (Amaryllidaceae) is an ornamental and medicinal plant. Its flowers and bulbs are used traditionally as a diuretic, emetic, against rashes and gonorrhea. The aim of this work was, to evaluate the antioxidant and antimicrobial activities of bulbs and bulbils alkaloid extracts of P. tuberosa. Antiradical effect was assessed against DPPH radical. However, antimicrobial activity was measured through the disc diffusion method against Escherichae coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Staphylococcus aureus resistant to Methicillin (MRSA) and Candida albicans ATCC 90028. The scavenging effect against DPPH showed that the bulbs and bulbils alkaloids extracts exhibited an antiradical effect with IC50 = 0.231±0.017 mg/mL and 0.233±0.093 mg/mL respectively, less than the effect of vitamin C with IC50 = 0.0194±0.0002 mg/mL. Antimicrobial activity results reveal that both alkaloid bulbs extracts at 50 mg/mL did not have any inhibitory effect against the studied strains using the disc diffusion method. According to this work, bulbs and bulbils alkaloid extracts show a moderate antioxidant effect; that could be recommended as a natural antioxidant. Although tuberose bulbs were used traditionally as a soap substitute; bulbs alkaloid extract has no antimicrobial effect. Keywords: Polianthes tuberosa L., bulbs, bulbils, alkaloids, antiradical activity, antimicrobial activity.

scholarly journals Screening of the antimicrobial activity of marine sponge extracts from Martinique

2021 ◽  
Author(s):  
Julie Piron ◽  
Jessica Pastour ◽  
Niklas Tysklind ◽  
Juliette Smith-Ravin ◽  
Fabienne Priam
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Marine Sponges ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Bacterial Strains ◽  
Disc Diffusion Method ◽  
Staphylococcus Saprophyticus

AbstractMarine sponges are known for their antimicrobial, antifungal, and cytotoxic activity. In this study, the activity of aqueous and ethanoic extracts of 3 sponges from Martinique were tested on 5 bacterial strains: Bascillus cereus (CIP 783), Echerichia coli (CIP 54127), Pseudomonas aeruginosa (CIP A22), Staphylococcus aureus (CIP 67.8) and Staphylococcus saprophyticus (CIP 76125). The antimicrobial activity of Agelas clathrodes, Desmapsamma anchorata, and Verongula rigida, was demonstrated using the disc diffusion method and by determining the minimum inhibitory concentration and the minimum bactericidal concentration. The ethanoic extract of Agelas clathrodes had an inhibitory activity specifically on Staphylococcus aureus and Staphylococcus saprophyticus. No activity was observed for the other extracts. Further chemical analyses will be carried out in order to identify the active molecules of these sponges.

scholarly journals Antimicrobial Activity of some Honey Samples against Pathogenic Bacteria

2015 ◽  
Vol 34 ◽  
pp. 15-20 ◽  
Author(s):  
S. Mahendran ◽  
D. Kumarasamy
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Western Ghats ◽  
Pathogenic Bacteria ◽  
Research Work ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method

The aim of the present research work to investigate antimicrobial activity of some honey samples six winter honeys six summer honeys collected from different regions of Western Ghats. The microbes used in this study are Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis. Antibacterial activity of the honeys was assayed using the Disc diffusion method. Noticeable variations in the antibacterial activity of the different honey samples were observed. Among the microbes Staphylococcus aureus is the most sensitive against all honey samples shows the maximum inhibitor zone compare to summer honeys.

Note: Inhibition of Pathogenic Bacteria by Essential Oils at Different Concentrations

2003 ◽  
Vol 9 (2) ◽  
pp. 85-88 ◽  
Author(s):  
G. Özkan ◽  
O. Sağdiç ◽  
M. Özcan
Keyword(s):  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antibacterial Agents ◽  
Antimicrobial Effect ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
E Coli ◽  
Paper Disc

The antimicrobial effect of 11 selected Turkish spice essential oils was investigated against seventeen pathogenic bacteria. The antimicrobial activity of the essential oils of six spices (cumin, fennel, laurel, mint, marjoram, oregano, pickling herb, sage, savory, thyme (black) and thyme) was tested at four concentrations (0.2, 0.4, 1 and 2%) on various microorganisms ( E. aerogenes, E. coli, E. coli O157:H7, K. pneumoniae, P. vulgaris, S. enteritidis, S. gallinarum, S. typhimurium, S. aureus, Y. enterocolitica, A. hydrophila, C. xerosis, M. luteus, M. smegmatis, E. feacalis, P. aeruginosa and P. fluorescens). All preparations showed antibacterial activity against at least one or more bacteria. The inhibitory effect of the essential oils was evaluated through paper disc diffusion method. In general, the essential oils at 1 and 2% levels were effective. The most active essential oils were marjoram, thyme and oregano. According to the results, the studied essential oils potentially might be used as antibacterial agents to prevent the spoilage of food products, although further research is needed.

scholarly journals Antimicrobial Properties of 6-Bromoeugenol and Eugenol

2016 ◽  
Vol 53 ◽  
pp. 57-64
Author(s):  
Radia Mahboub ◽  
Faiza Memmou
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Inhibitory Concentration ◽  
Antimicrobial Properties ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Bacterial Strains ◽  
Disc Diffusion Method ◽  
Modest Activity

We have studied the antimicrobial properties of 6-bromoeugenol and eugenol by three strains:Pseudomonas aeruginosa(S1),Escherichia coli(S2) andStaphylococcus aureus(S3). We have determined the minimum inhibitory concentration (MIC) for a range of concentrations using the disc diffusion method. We note that all samples present an antimicrobial activity toward the tested bacterial strains at different concentrations (1, 0.5 and 0.25 mg/ml). The 6-bromoeugenol gives modest activity with (S1) and (S3). Eugenol reacts positively with thePseudomonas aeruginosa(S1) at all concentrations and with theEscherichiacoli(S2) at 0.5 mg/ml. We remark that thePseudomonas aeruginosa(S1) is the more sensitive strain thanEscherichiacoli(S2) andStaphylococcus aureus(S3). We have estimated the activity coefficient which has confirmed the antimicrobial activity of the different samples. So, 6-bromoeugenol has shown his efficiency as antimicrobial agent.

scholarly journals Study the Inhibitory Effect of Garlic Extract on S. aureus and E. coli of MultiResistance to Antibiotics.

2015 ◽  
Vol 9 (1) ◽  
pp. 57-61
Author(s):  
Batool Hazim Al-Daherie Al-Daherie ◽  
Oruba Khalid Abbas Abbas
Keyword(s):  
Staphylococcus Aureus ◽  
Multidrug Resistance ◽  
Antibacterial Effect ◽  
Inhibitory Effect ◽  
Garlic Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Ofstaphylococcus Aureus ◽  
Disc Diffusion Method ◽  
E Coli

This Study aimed to investigate the effect of garlic extract against multidrug resistance ofStaphylococcus aureus and E. coli isolated from urine. Isolates were identified by usingbiochemical tests. The susceptibility of these microorganisms towards the most effectiveantibiotics were studied by using disc diffusion method. The multidrug resistancemicroorganisms were challenged with garlic extract using serial dilutions and the technology ofELISA spectrophotometer. The results of this study showed that ten isolates of E. coli and eightisolates of Staphylococcus aureus out of sixty isolates were resistant to all antibiotics. Garlicextract significantly inhibits the growth of these bacteria in a concentration independent manner.This study showed that garlic extract exerts a promising antibacterial effect against multidrugresistance of Staphylococcus aureus and E. coli.

scholarly journals ANTIMICROBIAL EFFICACY OF CARABAO GRASS LEAVES ON STAPHYLOCOCCUS AUREUS

2019 ◽  
Vol 7 (1) ◽  
pp. 384-397
Author(s):  
Dani Aiko Perral Garduque ◽  
Kyrie Radiance Ganal Mateo ◽  
Shalom Moyinoluwa Adejumoke Oyinloye ◽  
Jo Anne kristine Lalas Lucero
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
The Philippines ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Treatment And Prevention ◽  
Testing Center

Antimicrobial Efficacy of Carabao Grass (Paspalum conjugatum) leaves on Staphylococcus aureus Dani Aiko Garduque1, Kyrie Radiance Mateo1, Shalom Moyinoluwa Oyinloye1, and Jo Anne Kristine Lalas-Lucero2   1Student, Adventist University of the Philippines 2Faculty, Adventist University of the Philippines   [email protected]   Abstract   Introduction: In the Philippines, the Paspalum conjugatum or known by its common name as “carabao grass” is usually consumed for livestock as well as for landscaping. Paspalum conjugatum has been known to have phytochemicals that kill microorganisms. This experimental research determined the antimicrobial efficacy of Paspalum conjugatum against Staphylococcus aureus.   Methods: Samples of the carabao grass was purchased, washed and then dried. An extract was obtained from the sample. The extract was then sent to an accredited testing center for phytochemical test to identify the different components of the plant extract. The antimicrobial activity of the paspalum conjugatum extract was then determined through the use of the disc diffusion method. The zone of inhibition of Paspalum Conjugatum was also compared to a control standard such as oxacillin.   Results: The results of the study revealed to have abundant constituents of sterols, flavonoids and tannins; moderate levels of triterpenes, saponins and glycosides; and traces of alkaloids. The antimicrobial testing showed results that Paspalum Conjugatum had 10mm zone of inhibition which means that it has a complete inhibitory activity with severe reactivity against the tested organism, staphylococcus aureus.   Discussion: The study concludes that Paspalum conjugatum can be used to eliminate staphylococcus aureus. It is recommended that further studies be conducted for the use of Paspalum conjugatum in the treatment and prevention of diseases caused by staphylococcus aureus. Keywords:  paspalum conjugatum, carabao grass, staphylococcus aureus  

scholarly journals Antimicrobial Activity of Some Medicinal Plants from East and Central Part of Nepal

2014 ◽  
Vol 2 (1) ◽  
pp. 88-92 ◽  
Author(s):  
Bimala Subba ◽  
Prakash Basnet
Keyword(s):  
Antimicrobial Activity ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Proteus Vulgaris ◽  
Disc Diffusion Method ◽  
E Coli ◽  
Ethanolic Extracts ◽  
Inhibitory Activities

To evaluate the antimicrobial activities of extract of eleven plants were examined against four common bacterials. The ethanolic extracts of various plants such as  Cissus repens, Hedyotis scandens, Jatropha curcas, Morus alba, Inula cappa, Equisetum ramosissimum, Osyris wightiana, Alternantheria sessilis and Hibiscus lampas  investigated individually for antimicrobial activity by disc diffusion method. These were investigated against selected species of Staphylococcus aureus, Escherichia coli, Proteus vulgaris and Klebsiella pneumoniae to find the inhibitory activities of the microbes. The ethanolic extract of C. repens showed considerably high activity against P. vulgaris, E. coli and S. aureus than other extracts.DOI: http://dx.doi.org/10.3126/ijasbt.v2i1.9697Int J Appl Sci Biotechnol, Vol. 2(1): 88-92

scholarly journals ISOLASI DAN AKTIVITAS ANTI MIKROBA BAKTERI ASAM LAKTAT DARI FERMENTASI NIRA KELAPA SAWIT

2018 ◽  
Vol 5 (1) ◽  
pp. 51 ◽  
Author(s):  
Edy Fachrial ◽  
Harmileni Harmileni
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Palm Oil ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method

<p><em>The aim of the study was to isolate lactic acid bacteria (LAB) from palm oil sap and to investigate the anti microbial activity of LAB against pathogen bacteria, Staphylococcus aureus and Escherichia coli using disc diffusion method. Palm oil sap were enriched using spesific medium MRS (Man Rogosa Sharpe) broth overnight, serial dilution were conducted and the culture were spread on the surface of MRS agar and then incubated for 48 hours. The result showed the total colonies of LAB were 1,4 x 107 CFU/mL. 6 colonies were randomly selected namely EHN1, EHN2, EHN3, EHN4, EHN5 and EHN6. The selected colonies are classified as Gram positive and bacilli in morphology. Antimicrobial activity against pathogenic bacteria was investigated using disc diffusion method. Antimicrobial activity of isolates against E.coli and S.aureus were 12,2 mm; 9mm; 11mm; 11mm; 10,5mm; 12 mm dan 13,5mm; 11mm; 12mm; 11,5mm; 12mm; 12,5mm respectively. from these result it can be concluded that LAB isolated from oil palm sap has potential as probiotic.</em></p>

scholarly journals Synthesis and Antimicrobial Activity of Piperine Analogues Containing 1,2,4-Triazole Ring

2019 ◽  
Vol 31 (5) ◽  
pp. 1077-1080
Author(s):  
Kottakki Naveen Kumar ◽  
Karteek Rao Amperayani ◽  
V. Ravi Sankar Ummdi ◽  
Uma Devi Parimi
Keyword(s):  
Antimicrobial Activity ◽  
Triazole Ring ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Antibacterial Study ◽  
Gram Negative ◽  
E Coli ◽  
Micro Organisms

A series 1,2,4-triazole piperine analogues (TP1-TP6) were designed and synthesized. The structures were confirmed using 1H NMR and 13C NMR. Antibacterial study was done using Gram-positive (Staphylococcus aureus and Bacillus cereus) and Gram-negative micro-organisms (E. coli and Pseudomonas aeruginosa) by disc diffusion method. Compound containing chloro substitution (TP6) showed the highest effect, while compound TP1, TP3, TP4, TP5 showed the moderate activity.

scholarly journals Chemical Composition and Antibacterial Activity of Essential Oil of Anvillea radiata Coss. & Dur

2007 ◽  
Vol 2 (5) ◽  
pp. 1934578X0700200
Author(s):  
Fadwa El Hanbali ◽  
Ahmed El Hakmaoui ◽  
Fouad Mellouki ◽  
Lahoussine El Rhaffari ◽  
Mohamed Akssira
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Aerial Parts

The essential oil of the aerial parts of Anvillea radiata Coss. & Dur. was examined by GC-MS. Twenty-nine constituents were identified, representing 88.8% of the total. 6-Oxocyclonerolidol (66.6%) and 6-hydroxycyclonerolidol (11.4%) were found to be the major components. The disc diffusion method, used for antimicrobial activity testing, showed that the essential oil had a significant antibacterial activity against all tested microorganisms.

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